TW200936177A - Method for extracting using glacial water and the extract thereof, and cosmetic composition comprising nanoemulsion particles having the extract encapsulated or the glacial water - Google Patents

Abstract

The present invention relates to a method for extracting an active ingredient by using glacier water, accumulated for a long period of time, as an extraction solvent, an extract thereof, and a cosmetic composition comprising nanoemulsion particles having the extract encapsulated by the nanoemulsion technology to improve transdermal permeation, in which the extracts contain various minerals or active ingredients. Also, the present invention relates to a cosmetic composition comprising glacier water as an active ingredient to supply moisture to the skin, to reduce loose keratin and to soften the skin texture.

Description

200936177 The glacial water has a structure that allows microorganisms or diseases to be M, although M is colonized, and has a proportion of hexagonal water. 1 , ; , , 3 also have 1 large mineral. Therefore, areas where ice/water can be produced are known as longevity towns. Minerals maintain the osmotic pressure and acid balance in the body, control the body's wounds and metabolic functions, and play a heavy (four) color in oxygen transmission and cells. Therefore, the study of _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ According to the conventional method, the extraction step comprises mixing the target material with water, an organic solvent such as ethanol, m, butanol, ethyl acetate and chloroform or a mixture of water and an organic solvent, and placing the mixture at room temperature for days. Subsequent to the extraction, at least two: Newton extracts are repeated. The extract was visualized and the resulting filtrate was concentrated in a vacuum concentrator to give a crude product. The primary product is added with water and an organic solvent such as ethanol, decyl alcohol, butanol, ether, and acetonitrile, and placed at room temperature for 2 hours to separate the phases. Remove the aqueous phase and add organic solvent. This step is repeated at least twice to achieve adequate cleaning followed by filtration. The resulting liquid was dried in a vacuum oven to obtain the desired extract. This conventional solvent extraction method has several problems, such as problems related to the safety of residual organic solvents and the problems associated with the formulation and stability of the cosmetic product combination, and therefore requires a novel solvent extraction method to solve these problems. It is also highly desirable to develop solvent extraction methods to increase the absorbency of active ingredients such as minerals while ensuring safety. SUMMARY OF THE INVENTION Accordingly, the inventors of the present invention conducted research to solve the problem of skin fullness caused by residual organic solvents in the past, and to find a solvent to replace an organic solvent. As a result, the inventors found that when glacial water is used instead of water or an organic solvent to extract the active ingredient, the skin is kept safe while increasing the absorbency of the active ingredient. Further, the nano-emulsified granules are prepared by encapsulating the extract prepared therefrom by nanotechnology, and a cosmetic composition having improved transdermal absorbability can be provided. The present invention has been completed based on this development. Furthermore, the inventors of the present invention found that the molecular structure of glacial water is similar to that of water molecules found in living tissues, and thus can be absorbed through the skin, resulting in an excellent moisturizing effect. The present invention has been completed based on this finding. Accordingly, it is an object of the present invention to provide an extraction method of an active ingredient which is safely applied to the skin and which is excellent in absorbability, and an extract obtained therefrom, and an extract containing the extract as an active ingredient Cosmetic composition. Still another object of the present invention is to provide a cosmetic composition for increasing skin moisturizing effect, reducing keratin formation, and smoothing skin texture. In order to achieve the above object, the present invention provides a method for extracting an active ingredient using an ice which has been accumulated for a long period of time as an extraction solvent, an extract thereof, and a makeup. The sputum, 'and the composition, contains nano emulsified particles having a nano-encapsulation technique for improving percutaneous penetration, wherein the extract contains various minerals or active ingredients. Further, the present invention provides a cosmetic composition containing glacial water as an active ingredient. According to the present invention, the extraction of the active ingredient is carried out using glacial water rich in natural minerals. As a result, safety can be provided when applied to the skin, increasing the effectiveness of the active ingredient. Further, the percutaneous composition of the composition of g is maximized by the inclusion of the money skin affinity emulsion through the encapsulation. Further, the composition containing the glacial water according to the present invention has an excellent skin spreading effect and an increase in skin water content, thereby reducing the degree of seizure and smoothness and smoothing the surface of the skin. The above and other objects, features, and advantages of the present invention will be apparent from the accompanying drawings & 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 。 The invention will be described in detail below. The glacial water used in accordance with the present invention may include Hmiza 〇f Himalaya from the Himalayas, vilcabamba 〇f Eeuad (^ or Abkhasia of Chamgasso). Any of the Caucasus), preferably the Himalayan glacial water produced in Husa, Umalaya. The Himalayan glacial water, also known as "Hunza water", has a unique nanometer size II. a colloidal structure of cerium oxide. Therefore, it has a low surface tension and thereby enables the active ingredient to be immediately absorbed into the human body or the skin. The Himalayan glacial water also has a bird-shaped molecular structure similar to a hemispherical dome in 200936177. Capturing a large amount of hydrogen. These water molecules, σ 八 八 has a small surface-potential (zeta p〇tential) colloidal inorganic compound particles. It is known here that the water molecules of Qingcheng are quite old in living tissue. Water molecules, but completely different from water molecules found in general mineral water and tap water. According to the present invention, there is provided a method for solvent extraction using glacial water. The method comprises the steps of: ❹ 1) practicing extraction steps The resulting filtrate is obtained by concentrating the concentrated filtrate at a concentration of at least H and obtaining the primary product, wherein the extraction step comprises immersing the plant in glacial water at room temperature and filtering; and 2) adding glacial water to the product, and stirring the mixture at room temperature At least 2 hours, followed by thorough washing, filtering the mixture and drying in a vacuum oven to give an extract. In the present invention, glacial water has undergone natural filtration devices such as granite, sand butterfly layer and fine sand when it is melted into water. The filtered glacial water is a subterranean Xinshui aquifer that is obtained thousands of meters deep underground. It is clean and pollution-free due to the protection of natural barriers in the granite layer. Therefore, unlike the conventional method using an organic solvent, the solvent extraction method using glacial water according to the present invention simplifies the filtration step, whereby the extract is easily produced. Contains effective minerals such as sodium, magnesium, potassium and calcium as well as zinc, copper, hunger and glacial water that is immediately absorbed through the skin. The glacial water is also effectively purified by removing toxic substances, so that microorganisms or bacteria cannot reproduce therein. Therefore, according to the present invention, a cosmetic composition can be used regardless of the type of skin of the user, and can even be safely used for infants or people with skin problems such as patients with atopic dermatitis. 9 200936177 Active ingredients which can be extracted according to the method of the invention include, but are not particularly limited to, β-1,3-«hetero, lignin n 4, glandular ridge, vitamin c (ascorbic acid), arbutin, Acid amine and ethyl glucosamine. The glucan extracted according to the method of the present invention is a glucan having at least a high purity, is extracted from Schizophyllum c〇mmune hyphae and is effective for preventing skin aging and curing injured skin. In addition to Schiz〇 phylhim commune, 'β-ΐ, 3_glucan can also be extracted from Ganoderma lucidum (Gan〇derma japonicum) and Brazilian mushroom (Agaricus❹ blazei Muill) according to the solvent extraction method of the present invention. , Lentinus edodes, sclerotinia sclerotiorum, yeast and barley. In addition, 'active ingredient papaya enzyme can be extracted from fruit or papaya tree, and polyphenols can be taken from red or purple flowers of green tea, coffee, strawberry, eggplant, red beans, etc., red wine, black beans or cocoa. Saponin can be extracted from beans, ginseng or hawthorn plants. Adenosine can be extracted from plants such as wild parsley, and vitamin C (ascorbic acid) can be extracted from Rubus Coreamis, persimmon leaves, Rosehip, grapefruit or red bell pepper (pimento), arbutin can be extracted from arborvitae (Arctostaphylos uva ursi), can be extracted from potato or avocado, and ethoxylated glucosamine can be extracted from jaundice [Phellodendron amurense BJJYK) anti) \ \ Dry bark of Phellodendnm chinense SCHNEID. Further, according to the present invention, the extract (active ingredient) extracted using glacial water can be used as a emulsification technique (4) Wei or micro-leathering, in order to increase the mineral content of the glacial water. The fine emulsified particles of the glacial hydrolysate are used to transfer the total weight of the granules to 5G wt%. When the amount is less than _咐It is not enough to express the effect of the active ingredient. The equivalent is more than %-%, and it is difficult to formulate. The nano-emulsified particles containing the extract according to the present n can be prepared by a conventional high-pressure emulsification method (for example, a nano-emulsification technique) at a pressure of 500 to a bar. It.

The emulsified particles of the root plate have a diameter of 1000 nm to 3 ,, preferably ', Ώ to 50 nm. The contact surface of the nanoemulsified granules or vesicles with the skin enhances and thus increases the area absorbed by the percutaneous. Because the interstitial space between the horny layer (sher mc_um) is about 5 〇 nm and the emulsion film of the emulsified particles is elastic, the emulsified particles according to the present electric ray can be immediately absorbed or diffused into the cells. The moon is fat. That is, the emulsified particles having an average diameter and fine to 5〇J11 prepared by the nano-emulsification technique increase the percutaneous penetration of the emulsified particles themselves, and increase the contact area of the emulsified particles with the skin, and penetrate and diffuse through the cells. Inter-fat, and extracts extracted using glacial water. Meanwhile, the emulsified granules according to the present invention may further contain an egg % lipid and an extract as an emulsifier, and the lecithin content is 〇.5 to $, preferably 2 to 4 wt%, based on the total weight of the emulsified particles. The lecithin content is effective in forming a stable formulation of the cosmetic composition. Egg granules contain unsaturated biliary compounds, such as phosPtoidylch〇iine, defatted and phlegm (lys〇ph〇spl ddylch), phosphatidylethanolamine and its hydrogenation 200936177 According to the present invention, the emulsified particles may also include various nonionic surfactants as an emulsification aid, preferably a fatty alcohol composite surfactant or a high molecular weight interface, and the weight ratio of the remaining lions is based on (4) fat. The weight and composition are from 1 to 5 times, preferably from 5 to 2 times, based on the weight of the selenium. [The present invention also provides a cosmetic composition containing an emulsified granule or an extract extracted from glacial water or The viscous granule or the micro granules in an amount of from 〇〇1 to 5 〇% by weight based on the total weight of the composition. When the content of the emulsified granules or vesicles is less than 〇 When 〇〇1 wt%, the active ingredient is recorded in the formula cap. When the content exceeds 5 Gwt%, it is difficult to formulate. Further, the 'cosmetic composition can be selected as the active ingredient. Further, 'a cosmetic composition containing glacial water is provided according to the present invention. The glacial water content is from 0 to 100% by weight based on the total weight of the composition. When the glacial water content is less than 0.1% by weight, the glacial water has poor efficacy. The cosmetic composition containing the glacial water according to the present invention can replenish moisture to the skin, reduce keratin in the skin, and reduce skin roughness, thereby softening the skin texture. 化妆品 The cosmetic composition according to the present invention can be formulated into various formulations without Special restrictions. For example, the formula includes skin softener, skintoner, skin lotion, nourishing cream, massage cream, essence, eye cream, eye serum, cleansing cream, cleansing Skin foam, cleansing lotion, cloth, powder, body lotion, body lotion, body lotion, body essence, make_up base, foundation, hair conditioner, shampoo Fine, moisturizing lotion, body cleansing agent, teeth 200936177 t and mouthwash (mouth gaggle), skin whitening makeup products or medical supplements such as car people cream and patch. Now with the following examples The present invention is described, but the scope of the present invention is not limited thereto. fMiQU吏 Preparation of β-u-dextran extract from glacial water Adding 1 kg of Scliizophylkmi commune dried hyphae to 4 L· Himalaya Glacier The water was stored at room temperature for 1 day and then extracted. This step was repeated at least twice to obtain an extract, and then the extract was filtered. The mash was concentrated in a vacuum concentrator to obtain 400 g of a primary product. 4 〇〇g of product was added. To 1 l of glacial water, stir at room temperature for at least 2 hours, thoroughly rinse and filter. The filtered product was dried in a vacuum oven to obtain about 30 g of a β-1,3-glucan extract containing 70% or more of Schizophyll's coimmme. Comparative Example 1

Step at least twice, ...W into the 1 h G main product, stir at room temperature at least 2 Knife. Remove the aqueous phase and add 丨L ethanol. Repeat this for full cleaning and Wei. In the product of Yi Bing_Lin Filter, the product was obtained from 200936177 to β-1,3-glucan extract. Example 2: _ Nano-Emulsifying 叙 匍 将 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 The pentanediol is mixed together and added to the solution of the β-1,3-glucan extract prepared in the example and dissolved in 1 〇g of ethanol, and then heated to 7 〇 to 75 C to complete the glutamate solution. . The resulting solution was mixed with water (65.95 g steamed crane water, 5 g glycerol and 0.05 g EDTA). The water fraction was preheated, pre-emulsified using a conventional homomixer (3 minutes, 3,000 to 6,000 rpm) and homogenized using a high pressure microjet. The microfluidizer was emulsified at 800 Bar/3 cycles. Comparative Example 2 4 g of hydrogenated lecithin, 4 g of PEG-5 rapeseed sterol, 5 g of polyethylene glycol, 5 g of pentanol and 10 g of ethanol were mixed and heated to 70 to 75 ° C To completely dissolve. The resulting solution was mixed with water (66.95 distilled water, 5 g glycerol and ED.5 g of EDTA), the water fraction was preheated, pre-emulsified using a conventional homomixer (3 minutes, 3,000 to 6,000 rpm) and high pressure microjets were used. The homogenizer was emulsified at 8 〇〇 Bar/3 cycle. Comparative Example 3 The β-1,3-glucose prepared in Comparative Example 1 200936177 was replaced by the β-1,3-glucan extract prepared only in Example 1. In addition to the polysaccharide extract, nanoemulsified granules were obtained. The same procedure as in Example 2 above was carried out to culture human fibroblasts in a 24-well plate. The medium was replaced with a medium containing the composition of the concentration described in the table. The cells were further cultured for 3 days. Each well was treated with (the special esoteric bovine serum should be treated with '3,4,5-3^^ aminic acid. After 24 hours, the medium and cells were collected from each well, with 5% trichloroacetic acid ( The TCA) solution was washed and dispensed into 2 tubes. Add i units/μΐ type t collagenase to the tube and incubate for 30 minutes at 3rc. The other tubes were stored at 4. Bu and then stored in 0.05 ml of 50% trichloroacetic acid in the tube. At 4t: 2 〇 minutes, centrifuge at 12, _ rpm for 1 。 minutes. Use the liquid scintillation counter (LSC) to separately measure the CPM (counts per minute) of the supernatant and the sink. Obtain the control group by the following equation i And the relative collagen biosynthesis (RCB) values of the treatment group. The results are shown in the following table i. [Equation 1] RCB [take, original protein CPM / { (total protein - collagen cpm) x5.4 + collagen Protein cm}]x 1〇〇200936177 Concentration (ppm) [Table 1] White biosynthesis (RCB) rate (%) 10 Control group 1 Example 1 ----, ------ Poor Example 2 ----- 100 —--- 120 "" Round ~--- 106 100 ---- 105 '--- 101 One, ... 丨 甘 甘 雒 雒 ; ; ;; 3_Portuguese ¥ Bio-sugar Preparation of extract Chennai hard material (concentration of compound at a final white gum increase Utah '' to 0

The skin lotion (unit: plus %) of the formula 丨 and the comparative formula} to 3 was prepared according to the conventional method using the ingredients described in Table 2 below. [Table 2] Ingredient Formulation 1 Comparative Formulation 1 Comparative Formulation 2 Comparative Formulation 3 Nanoemulsified particles prepared in Example 2 10.0..----Nano-emulsified particles prepared in Comparative Example 2 - 10.0 - - Comparative Example 3 Preparation of nano-emulsified particles - - 10.0 - Cetylethylhexanoate 5.0 5.0 5.0 —---- 5.0 16 200936177 ❹

Cetostearylalholhol 1.0 —----- »»-- 1.0 • One — 1.0 — 1.0 Lipophilic monostearate 0.8 ------ 0.8 0.8 ^―--- 0.8 Squalene 2.0 2.0 2.0 2.0 Polysorbate 60 1.5 „ --------- 1.5 1.5 —— 1.5 Sorbitan Sesquioleate 0.5 - --- 0.5 ~--'--- —'—— 0.5 0.5 Polyethylene glycol 5.0 5.0 5.0 ς Triethanolamine _______ 0.2 _____—^ —· — — 0-2 ----_ 0.2 0.2 Carboxyrene Polymer 0.2 — 0.2 — '—. —— 0.2 0.2 Preservative proper amount — Appropriate amount of appropriate amount of suitable coloring agent----" Appropriate amount~~~-- Appropriate amount of appropriate amount of spices-------- - Appropriate amount of purified water emperor 100 - - to 1 〇〇 - _ _ to 100 to 100 Optimistic Example 2: Effect of the scent of gold scented in animals _ To confirm the extraction with glacial water according to the present invention The effect of β-1,3-glucan on the biosynthesis of collagen was applied to the skin lotion prepared in Formulation 1 and Comparative Formulations 1 to 3 in the absence of 4^, rat back-week. Analyze the immunohistochemistry of collagen. Calculate the data from the comparison of 100 of Formulation 3, the data is untreated. The results are shown in Table 3. 17 200936177 [Table 3] Test Material Formula 1 Comparison Formula 1 Comparison Formula 2 " __________ Collagen biosynthesis (%) ------________ _ 135 105 ___________ _ 128 Comparison formula 3 ~~---- ------- _ 100

From the data in Table 3, the collagen biosynthesis in the group compared with the comparative formulas i and 2, the application formula was increased by σ 28 6% and 5 5%, respectively. Therefore, it was confirmed that the sugar extraction extract extracted from the Himamam glacial water according to the present invention increases the percutaneous absorption and increases the collagen biosynthesis. Appropriate Example 3: Measurement of Skin Elasticity Improvement on Skin The test was based on the skin elasticity improving effect of the skin lotion prepared in Table 2 above.

Twenty healthy women aged at least 30 were divided into two groups and were between 24 and 26. (: 75% with humidity. Each group of women applied Formula 1 or Comparative Formula 2 skin conditioning water to the face for 12 weeks. Then use the skin tester Cutometer SEM 575 (C+K

Electronic Co., Germany) measures skin elasticity. The data is the R8 value of the skin tester Cutometer SEM 575 [R8 (left) - R8 (right)], and the results are shown in Table 4 below. The R8 value represents the viscoelasticity of the skin. 18 200936177 [Table 4] Test material ------ Skin elasticity formula 1 ----- 0.33 Comparison formula 2 - circle --- 0.10 [Table 5] Test material responders are very good and generally poor formula 1 3 6 1 0 Comparative Formulation 2 1 3 3 3 Formulation 1 containing the β-1,3-glucan extract extracted from the Himalayan glacial water according to the present invention was also confirmed from the questionnaire to improve skin elasticity. Comparative Formulation 4: Preparation of Skin Conditioning Water The skin conditioning water (unit: wt%) of Formulation 2 and Comparative Formulation 4 was prepared according to the conventional method using the ingredients described in Table 6 below. The formula U, which contains the dextran extract extracted from the Himalayan glacial water according to the present invention, has a greater increase in elasticity. After the test, the participants were asked to answer the questionnaire separately for evaluation outside the mechanical evaluation. The results are shown in Table 5 below. Subjective skin subjective 200936177 [Table 6] Ingredient Formulation 2 Comparative Formulation 4 Glacial Water (Hansa Glacial Water) 3.00 - /3 from Example 1 - 1,3-Glucan Extract 1.10 - From Comparative Example 1 /3-1,3-Glucan Extract - 1.10 dinal EDTA 0.02 0.02 DL-vitamin B 5 (DL-pantenol) 0.05 0.05 butanediol 2.00 2.00 Glycereth-26 (6.00) 6.00 PEG .PPG-17.6 Copolymer 2.00 2.00 Propionic acid light ethyl s / propyl succinyl sulphate yellow copolymer 0.65 0.65 ethanol 5.00 5.00 bis-PEG-18 decyl ether decyl decane (didecyl)矽 烷 共聚 ) 0.2 0.2 0.20 0.20 Polyoxyethylene hydrogenated castor oil 0.30 0.30 Phenyltrimethicone 0.10 0.10 Preservatives appropriate amount of spices appropriate amount of purified water to 100 to 100 200936177 f mating Side 5: Cream Responsibility The ingredients of Formulation 3 and Comparative Formulation 5 (unit: wt〇/0) were prepared according to the conventional methods using the ingredients described in Table 7 below. [Table 7] Ingredient Formulation 3 Comparative Formulation 5 Glacial Water (Hansa Glacial Water) ----- 1.00 .. - β-1,3-glucan from Example 1 1.10 - β_1 from Comparative Example 1 , 3_glucan - 1.10 dinal EDTA 0.02 0.02 PEG-75 1.00 1.00 bis-PEG-18 mercapto dimethyl didecyl decane (dioxanoxane copolyol) 0.5 0.5 butanediol 5.00 5.00 glycerol (concentrated Glycerin) 2.00 ------ 2.00 Recording worm-based PEG.PPG-10.1 Diterpenoid Shixi Oxygen (Su*Mic-based bismuth-based oxy-copolymer) 0.500 0.500 PEG-7 ( 二 曱 石 氧 览 览 览 共聚 共聚 共聚 彳 彳 彳 彳 彳 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. 2. Base oxane) 4.00 4.00 Butanediol Dioctanoic acid / dicaprate 3.00 3.00 Preservative ~--- A proper amount of spices _______ Appropriate amount --------- Appropriate amount of purified water to 100 to 100 EXPERIMENTAL EXAMPLES Filling the skin to measure the application of a group containing glacial water (provided from the water of the Himalayas (Formula 2) or cream (Formulation 3)) And the skin of the skin-adjusted water (Comparative Formula 4) or cream (Comparative Formula 5) of the skin without ice cream, measure the moisture content of the skin and compare the results. Skin conditioning water and milk Creams are applied separately to different groups to test for increased moisture content caused by each product, and there is no interaction between products. Each group includes 20 healthy adult women. The forearm is cleaned and placed in the first visit. The skin was acclimated in a fixed temperature and humidity chamber (23 ° C ' RH 40%) for 30 minutes. After 30 minutes, the initial moisture content of the forearm skin was measured using a moisture tester (CM825, C+K Electronic Co., Germany). The instrument measures the moisture content of the skin by measuring the conductivity of the skin. The cosmetic composition of 2 μl/αι is then applied to the aforementioned forearm portion of 15 cn!. After 3 hours, 6 hours and 9 hours (including skin conditioning water) 12 hours), the skin part of each formula was measured for its moisture content. Compare the results obtained with the 22 200936177 group containing the glacial water product and the group using the glacial water-free product. As a result, the total moisturizing effect and water retention capacity of the product were determined. The results are shown in Table 8 (unit: AU) [Table 8] 〇 hours 3 hours 6 hours 9 hours 12 hours Formula 2 *". (Skin s Weekly water AD, including Glacial Water) 30 51 43 38 35 Compare Formula 4 (skin s weekly water, no glacial water) 31 46 38 35 33 Formula 3 (cream AD 'with glacial water) 26 68 55 51 - Compare Formula 5 — (Cream, Excluding glacial water) 26 58 50 46 - φ From the results of Table 8, it was found that the skin to which Formulations 2 and 3 were applied contained more moisture than the skin to which Comparative Formulas 4 and 5 were applied. EXPERIMENTAL EMBODIMENT 5: Lowering keratin by using the moisture of glacial water to reduce the oil of ginseng [As the amount of keratin is reduced, the skin conditioning water for applying glacial water (provided from the dry hills of the Himalayas) 2) or cream (Formulation 3) in the skin group, and application of skin conditioning water (Comparative Formula 4) or cream (Comparative Formula 5) 200936177 without glacial water's measurement in the skin by image analysis The amount of keratin was compared and the results were compared. Each group includes 20 healthy adult women. The forearm was cleaned during the first visit and was placed in a constant temperature and humidity chamber (23 C ‘ RH 40%) for 30 minutes to allow the skin to adapt. After 30 minutes, the keratin was removed using a tape to measure the initial keratin content of the forearm. Then, the composition of the forearm referred to in the above 15 ί is applied as a cosmetic composition such as "Μ. After 3 hours, 6 hours, and $ hours (including skin conditioning water for 12 hours), the skin part of the secret recipe was measured for keratin content. (4) Image analysis, recording the results obtained from the _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ Silky

And Figure 2 (unit: AU). / P [Table 9] 0 hours - 3 hours ----- 6 hours 9 hours 12 small days --~~~~'-- Formula 2 (skin conditioning water AD 'ice containing> water) —— 24 10 10 12 15 Compare Formula 4 (Skin Conditioning Water AD, excluding glacial water, 24 ----- 19 —'— 15 ------ 13 15 16 ---^. Formula 3 (cream AD, Containing glacial water) -—~~— 2 4 5 _ Comparative formula 5 (cream, no glacial water) '—~~~- 19 4 5 6 ❹ 24 200936177 From the above table 9 and the first and second figures As a result, it was found that Formulations 2 and 3 reduced the amount of keratin more than Comparative Formulas 4 and 5. Thus, the product containing glacial water was more effective in moisturizing and softening the skin. Experimental Example 6: Reducing skin roughness by using water supplement of glacial water Measurement of the application of skin conditioning water (formulation 2) or cream (formulation 3) containing glacial water (provided from Hunza in the Himalayas) to the skin group and application of skin conditioning water without glacial water (Comparative Formula 4) Or cream (Comparative Formula 5) skin roughness and compare the results. Each group includes 20 healthy adult women. The first visit will clean the forearm and be in place The skin was acclimated in a humidity chamber (23 ° C ' RH 40%) for 30 minutes. After 3 minutes, the skin roughness was measured using a 3D cat scanner, PRMOS compact (G.FM_, Germany), then 2μ1/αι彳The cosmetic composition was applied to the forearm portion referred to above in 15 cn. After 9 hours (including skin conditioning water for 12 hours), the skin roughness of the same portion of the skin was measured. Comparing the results and use of the group containing the glacial water product The results obtained from the group of glacial water products were not included, and it was confirmed that the product containing glacial water had an effect of smoothing the texture of the skin. The results are shown in Fig. 3 to Fig. 6. From the results of Fig. 3 to Fig. 6, the formula 2 was found. And Formula 3 reduces the skin roughness more than Comparative Formula 4 and Comparative Formula 5 'The product containing glacial water is more effective in smoothing the skin texture. 25 200936177

Applying skin conditioning water (Formulation 2) or cream (Formulation 3) to the skin group and applying skin conditioning water without glacial water (Comparative Formula 4) or from the application of skin containing glacial water (from Hunza in the Himalayas) Cream (Comparative Formula 5) Take a sample of the stratum corneum in the skin group and pre-treat. The roughness of the sample is then measured and the results are compared. Each group consists of 2 healthy adult women. The forearm was cleaned during the first visit and was placed in a fixed temperature and humidity room (23 C 'RH40%) for 30 minutes to allow the skin to adapt. After 3 minutes, take a sample of the stratum corneum (skin surface section). The forearm portion referred to before 15 was then applied with a cosmetic composition of 2 μΐ/Μ. After 9 hours (including skin conditioning water for 9 hours and 12 hours), the stratum corneum samples were taken in the same area, pretreated and the corneal layer was examined by electron microscopy. The results obtained by using the group containing the glacial water product by the car father and the result of using the group containing no hail water product, and confirming that the product containing the glacial water has the effect of smoothing the skin. The results are shown in Figures 7 through 1 . From the results of Fig. 7 to Fig. 10, it is found that Formulation 2 and Formulation 3 reduce the roughness of the stratum corneum more than Comparative Formulas 4 and 5. Thus, the product containing glacial water is more effective in smoothing the skin. The present invention has been described with respect to particular exemplary embodiments, but the invention is not limited to the specific examples and is limited only by the scope of the appended claims. It will be appreciated that those skilled in the art can change or modify the specific embodiments without departing from the scope and spirit of the invention. 26 200936177 [Simple description of the diagram] Figure 1 shows the amount of keratin after using Formula 2. Figure 2 shows the amount of keratin after using Formulation 4. Figure 3 shows the skin roughness after Formulation 2. Figure 4 shows the skin roughness after using Comparative Formula 4. Figure 5 shows the skin roughness after Formulation 3. ^ Figure 6 shows the skin roughness after using Formulation 5. Figure 7 is a photograph showing the stratum corneum roughness after Formulation 2. Figure 8 is a photograph showing the stratum corneum roughness after using Comparative Formula 4. Figure 9 is a photograph showing the stratum corneum roughness after Formulation 3. Figure 10 is a photograph showing the stratum corneum roughness after using Comparative Formula 5.

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Claims (1)

200936177 X. Patent application scope: 1. - A method for extracting active ingredients from plants using glacial water as an extraction solvent. 2. If you apply for a patent scope! The method of the present invention comprises the steps of: 1) repeating the stroke step at least twice and concentrating the obtained mash in a vacuum concentrator to obtain a primary product, the priming step comprising immersing the glacial water at room temperature! And filtration; and 2) adding glacial water to the product, stirring the mixture at room temperature for at least 2 hours, followed by a β wash, filtering the mixture and drying in a vacuum oven to obtain an extract. ❹ 3. For the method of claim 1, the glacial water is from Hmiza 〇f Himalaya in the Himalayas, Viicabamba 〇f Ecuador in Ecuador or Abel in the Caucasus. Abkhasia of Caucasus. 4. The method of claim 1, wherein the plant is at least one selected from the group consisting of Schizophyllumcommie 4^^(Ganodermajaponicum), C® Dust 4 (Agaricus blazei Muill), and Lentinus edodes. Sclerotinia sclerotiorum, yeast, barley, papaya, green tea, coffee, strawberry, eggplant, grape, red bean, black bean, cocoa, bean, ginseng, hawthorn, wild parsley, raspberry (Rubus Coreanus) , persimmon leaves, rosehip, grapefruit, red bell pepper (pimento), bearberry per (Arctostaphylos uva ursi), horse yam, pear and yellow ban (Phellodendronamurense Ruprecht) group. 5. The method of claim 1, wherein the active ingredient is at least one selected from the group consisting of: 28 200936177 β-1,3-glucan, papaya enzyme, polydatin, saponin, adenosine, vitamin C (ascorbic acid) Groups of arbutin, nicotinamide and ethionyl glucosamine. 6. A nano-emulsified granule comprising the active ingredient extracted according to the method of the scope of the patent application and lecithin' and having a diameter of from 1 to 3 〇 nm. 7. The nanoemulsified granule according to item 6 of the patent application, wherein the active ingredient content is 0.001 to 50 wt% based on the total weight of the emulsified granules. © 8· For example, the nano-emulsified granules of the sixth paragraph of the Chinese patent range have a phospholipid content of 0.5 to 5 wt0/〇 based on the total weight of the nano-emulsified particles. A cosmetic composition comprising as an active ingredient a nanoemulsified granule according to any one of claims 6 to 8. 10. For the nano-emulsified granules of claim 9 of the patent scope, the step further comprises glacial water as an active ingredient. ® u. A cosmetic composition containing glacial water as an active ingredient. 12. The cosmetic composition of claim U, wherein the glacial water content is from 0.1 to 〇〇 wt% based on the total weight of the composition rf*. η. A cosmetic composition according to claim 11 of the patent scope, wherein the glacial water is produced from Hunza of Himalaya in the Himalayas, VUcabamba 〇f Ecuador in Ecuador or Abel in the Caucasus. Abkhasia 〇f Caucasus ° 200936177 14. A cosmetic composition according to claim 11 for moisturizing the skin. 15. The cosmetic composition of claim 11 for use in the reduction of keratin. 16. The cosmetic composition of claim 11 which is used to alleviate the thick chain of the skin surface. 30 200936177 V. Abstract of the Invention: The present invention relates to a method for drawing an active ingredient using ice which has accumulated a long time of ice and water as an extraction solvent, and the extract thereof, as well as the seeding, are also produced and have Nano-emulsified granules are used to improve the transdermal permeation of nano-emulsified granules enclosed in s-heavy extracts, wherein the extract contains various minerals or active ingredients. The present invention also relates to a cosmetic composition containing glacial water as an active ingredient for replenishing skin moisture to reduce keratin looseness and soften skin texture. The present invention relates to a method for extracting an active ingredient by using glacier water, accumulated for a long period of time, as an extraction solvent, an extract thereof, and a cosmetic composition comprising nanoemulsion particles having the extract Encapsulated by the nanoemulsion technology to improve transdermal permeation, in which the extracts contain various minerals or active ingredients. Also, the present invention relates to a cosmetic composition comprising glacier water as an active ingredient to supply moisture to the skin, to reduce loose keratin and To soften the skin texture. VII. Designated representative map: None (1) The representative representative map of this case is: ( ). (2) A brief description of the symbol of the representative figure: 200936177 VIII. If there is a chemical formula, please solve the chemical formula of the most __ characteristics. Illustrated in the invention: [Technical field of invention] β #本发明是A method for extracting an active ingredient from a long-term glacial water as an extraction solvent, an extract thereof, and a cosmetic composition containing a nano-emulsified encapsulated with the nano-emulsification technique for improving percutaneous penetration Granules wherein the extract contains various minerals or active ingredients. The present invention also relates to a cosmetic composition containing glacial water as an active ingredient for replenishing skin moisture to reduce keratin looseness and soften skin texture. [Prior Art] Ο In the mountains or polar regions, snow has accumulated for a long time without melting to form permafrost, and its pressure is transformed into hard ice caps. The water produced in the text from the ice cap is called "glacier water." At present, glacial water is formed in the polar regions, which is one of the cleanest areas on the earth or high mountains. This means healthy and natural pure vitality. Representative glacial water production areas include Vilcabamba of Ecuador in Ecuador, Abkhasia of Caucasus in the Caucasus, or Hunza of Himalaya in the Himalayas. 5