KR20220056539A - A novel biological network mimic method for extraction of useful ingredients from biological raw material - Google Patents
A novel biological network mimic method for extraction of useful ingredients from biological raw material Download PDFInfo
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- KR20220056539A KR20220056539A KR1020200141222A KR20200141222A KR20220056539A KR 20220056539 A KR20220056539 A KR 20220056539A KR 1020200141222 A KR1020200141222 A KR 1020200141222A KR 20200141222 A KR20200141222 A KR 20200141222A KR 20220056539 A KR20220056539 A KR 20220056539A
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- extraction
- water
- extract
- present
- phospholipids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/208—Fungi extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Abstract
Description
본 발명은 생물학적 원료로부터 유용성분을 추출하기 위한 신규한 생체 네트워크 모방 추출 방법에 관한 것이다. 보다 구체적으로, 본 발명은 추출 원료의 수용성 및 유용성 효능 성분을 동시에 추출하면서 물질간 네트워크가 생체에서와 유사하게 유지될 수 있도록 막 구조를 생성시키는 원리를 이용한 새로운 추출 방법에 관한 것이다.The present invention relates to a novel biological network mimic extraction method for extracting useful components from biological raw materials. More specifically, the present invention relates to a new extraction method using the principle of generating a membrane structure so that the network between substances can be maintained similarly to that in the living body while simultaneously extracting the water-soluble and oil-soluble effective components of the extraction raw material.
식물, 동물, 미생물 등 생물학적 원료로부터 유용성분을 추출하여 화장료 조성물, 식품 조성물 또는 약학 조성물에 이용하고자 하는 노력이 지속되고 있으나, 이에 대한 연구는 수세기 동안 원료 내 하나 또는 몇몇의 활성 성분이 생리활성을 결정한다는 가정 하에 주로 하나의 활성성분을 찾으려는 방향으로 이루어지고 있었다. 그러나 최근 연구 보고에 의하면 인체에 유효하다고 알려져 있는 비타민 및 항산화물질에 대한 임상시험 연구 결과를 종합하여 분석하는 메타분석 연구 결과, 비타민 단독 또는 항산화 물질 단독으로는 생물학적 효과를 낼 수 없다는 것이 밝혀지고 있다. 이에 반해 채소나 과일을 그대로 섭취한 임상시험에 대한 메타분석에서는 유효성이 나타나는데, 이는 비타민과 항산화 물질이 생체에서는 단독으로 작용하는 것이 아니라 복합체의 형태로 네트워크를 이루어 작용하기 때문인 것으로 해석되고 있다. 또 다른 연구들에 의하면 비타민 복합체도 그 자체로는 유효성을 나타내지 못하는데, 이는 비타민 복합체도 긍정적인 효능을 위해서는 과일과 채소의 다른 영양성분이 함께 작용해야 한다는 것을 제시한다. 원료 자체가 분리된 각각의 유용성분 보다 우수한 효능을 갖는 이유는 원료 내에 존재하는 여러 가지 성분들, 예를 들어 세포막에서 발견되는 지용성 물질들, 세포의 수용성 부분들로부터 나온 극성 물질들, 산성/염기성 이온들, 안정한 구조물과 산화된 구조물 등이 복합적으로 작용한 결과라고 할 수 있다. 따라서 원료의 유용성분을 포함하는 조성물에서 기대하는 효과를 얻기 위해서는 생물학적 원료로부터 얻어지는 성분의 총체적 데이터가 유용성분으로서 반영되어야 할 것이다.Efforts have been made to extract useful ingredients from biological raw materials such as plants, animals, and microorganisms and use them in cosmetic compositions, food compositions, or pharmaceutical compositions, but research on this has been ongoing for centuries. Under the assumption that the decision was made, it was mainly done in the direction of finding one active ingredient. However, according to a recent research report, as a result of a meta-analysis study that synthesizes and analyzes the results of clinical trial studies on vitamins and antioxidants known to be effective in the human body, it is revealed that vitamins alone or antioxidants alone cannot produce biological effects. . On the other hand, the meta-analysis of clinical trials in which vegetables or fruits were consumed as they were showed efficacy. Other studies have also shown that vitamin complexes are not effective on their own, suggesting that vitamin complexes must work together with other nutrients in fruits and vegetables for positive efficacy. The reason that the raw material itself has superior efficacy than each isolated useful ingredient is that various ingredients present in the raw material, for example, fat-soluble substances found in cell membranes, polar substances from water-soluble parts of cells, acidic/basic It can be said that it is the result of a complex action of ions, stable structures and oxidized structures. Therefore, in order to obtain the expected effect from the composition including the useful component of the raw material, the total data of the ingredient obtained from the biological raw material should be reflected as the useful ingredient.
한편, 종래 생물학적 원료로부터의 유용성분 추출 방법은 단일 용매 추출 또는 2 내지 3종의 용매를 혼합한 단일계 혼합 용매 추출로서 원료의 효능성분이 통합적으로 추출되지 않고 추출용매에 대한 용해도에 의해 단순 분획 추출될 뿐이므로, 세포에서와 같은 물질 간 네트워크가 반영된 추출물이 제조되지 않는다는 문제점이 있었다. 이에 원료로부터 보다 효과적으로 유용성분을 추출하기 위해서는 원료의 수용성 및 지용성 효능 성분을 동시에 추출하면서 물질 간 네트워크가 생체에서와 유사하게 유지될 수 있도록 하는 새로운 추출 기술이 요구된다.On the other hand, the conventional method for extracting useful components from biological raw materials is a single solvent extraction or a single-system mixed solvent extraction in which two to three solvents are mixed, so that the effective components of the raw materials are not extracted integrally, but simple fractionation by solubility in the extraction solvent Since it is only extracted, there is a problem in that an extract reflecting the network between substances such as in cells is not prepared. Accordingly, in order to more effectively extract useful components from raw materials, a new extraction technology is required to simultaneously extract water-soluble and fat-soluble active ingredients of raw materials while maintaining the network between substances similar to those in the living body.
따라서 상기 제기된 문제를 해결하기 위하여 각고의 노력을 지속한 끝에 본 발명을 완성하였다. 본 발명은 세포에서의 물질 간 네트워크 등 식물, 동물, 미생물 등과 같은 생체를 복합적으로 반영 가능한 신규 생체 네트워크 모방 추출방법에 관한 것으로, 본 발명의 추출 방법으로 추출된 유용성분은 의학, 바이오, 미용, 식품 등의 다양한 분야에서 활발하게 이용될 것으로 기대된다.Therefore, the present invention was completed after continuous efforts to solve the problems raised above. The present invention relates to a novel biological network imitation extraction method capable of complexly reflecting living organisms such as plants, animals, microorganisms, etc., such as networks between substances in cells, and useful ingredients extracted by the extraction method of the present invention include medicine, bio, beauty, It is expected to be actively used in various fields such as food.
따라서 본 발명의 주된 목적은 생체 내에서의 물질 간 네트워크가 반영될 수 있도록 관련 성분들을 추출함으로써 이들 성분들의 네트워크를 통해 추출물에서 우수한 생리활성을 나타낼 수 있도록 하는 새로운 추출 방법을 제공하는데 있다.Therefore, the main object of the present invention is to provide a new extraction method that enables the extract to exhibit excellent physiological activity through the network of these components by extracting the relevant components so that the network between substances in the living body can be reflected.
본 발명의 다른 목적은 상기 추출 방법을 통해 얻어지는 우수한 생리활성을 나타낼 수 있는 추출물을 제공하는데 있다.Another object of the present invention is to provide an extract capable of exhibiting excellent physiological activity obtained through the extraction method.
본 발명의 또 다른 목적은 상기 추출물을 포함하여 우수한 생리활성을 나타낼 수 있는 화장료 조성물 또는 식품 조성물을 제공하는데 있다.Another object of the present invention is to provide a cosmetic composition or a food composition that can exhibit excellent physiological activity, including the extract.
본 발명의 한 양태에 따르면, 본 발명은 추출 원료에 인지질, 계면활성제, 인지질 이외의 지질, 수용성 용매 및 물을 가하고 교반하는 단계를 포함하는 추출 방법을 제공한다.According to one aspect of the present invention, the present invention provides an extraction method comprising the step of adding and stirring phospholipids, surfactants, lipids other than phospholipids, water-soluble solvents and water to the extraction raw material.
본 발명의 추출 방법에 있어서, 상기 단계는 상기 인지질, 계면활성제 및 인지질 이외의 지질을 혼합하여 유상부를 제조하는 단계; 상기 수용성 용매 및 물을 혼합하여 수상부를 제조하는 단계; 및 상기 추출 원료에 상기 유상부 및 수상부를 가하고 교반하는 단계;를 포함하는 것이 바람직하다.In the extraction method of the present invention, the step comprises the steps of preparing an oil phase by mixing lipids other than the phospholipids, surfactants and phospholipids; preparing an aqueous phase by mixing the water-soluble solvent and water; and adding the oil phase part and the aqueous phase part to the extraction raw material and stirring.
본 발명의 추출 방법에 있어서, 상기 계면활성제는 스팬(span)형 계면활성제, 트윈(tween)형 계면활성제 및 비이온성 계면활성제로 이루어진 군으로부터 선택되는 하나 이상인 것이 바람직하다.In the extraction method of the present invention, the surfactant is preferably at least one selected from the group consisting of span-type surfactants, twin-type surfactants and nonionic surfactants.
본 발명의 추출 방법에 있어서, 상기 인지질 이외의 지질은 탄소수 12 이상의 지질인 것이 바람직하다.In the extraction method of the present invention, lipids other than the phospholipids are preferably lipids having 12 or more carbon atoms.
본 발명의 추출 방법에 있어서, 상기 인지질 이외의 지질은 지방산 에스테르 및 식물성 오일로 이루어진 군으로부터 선택되는 하나 이상인 것이 바람직하다.In the extraction method of the present invention, the lipid other than the phospholipid is preferably at least one selected from the group consisting of fatty acid esters and vegetable oils.
본 발명의 추출 방법에 있어서, 상기 수용성 용매는 탄소수 2 내지 6의 알코올인 것이 바람직하다.In the extraction method of the present invention, the water-soluble solvent is preferably an alcohol having 2 to 6 carbon atoms.
본 발명의 다른 양태에 있어서, 본 발명은 상기 추출 방법으로 추출된 추출물을 제공한다.In another aspect of the present invention, the present invention provides an extract extracted by the above extraction method.
본 발명의 또 다른 양태에 있어서, 본 발명은 상기 추출물을 포함하는 화장료 조성물을 제공한다.In another aspect of the present invention, the present invention provides a cosmetic composition comprising the extract.
본 발명의 또 다른 양태에 있어서, 본 발명은 상기 추출물을 포함하는 식품 조성물을 제공한다.In another aspect of the present invention, the present invention provides a food composition comprising the extract.
본 발명에 따르면 기존의 용매 추출 방법으로 유효성분을 추출하여 수득한 추출물 또는 용매 추출 이후 유효성분을 리포솜 또는 나노에멀션 입자에 포집하여 수득한 물질과 비교하여 보다 우수한 생리활성을 갖는 추출물을 제조할 수 있다. 이는 본 발명의 추출 방법이 추출과 유화, 미세이중상 입자 형성이 함께 이루어지도록 하여 추출 원료로부터 수용성 및 지용성 성분, 뿐만 아니라 세포막에 존재하는 성분들도 추출되도록 하고, 각 성분들이 추출 원료에서 존재하는 것과 유사한 상(phase)에 안정적으로 존재하도록 하여 이들 성분들의 네트워크를 발휘할 수 있도록 하기 때문인 것으로 생각된다. 본 발명의 추출 방법으로 수득되는 추출물은 이와 같이 우수한 생리활성을 가지므로 의학, 바이오, 미용 및 식품 등의 다양한 분야에서 유용할 것으로 기대된다.According to the present invention, it is possible to prepare an extract having better physiological activity compared to an extract obtained by extracting the active ingredient by a conventional solvent extraction method or a substance obtained by collecting the active ingredient in liposomes or nanoemulsion particles after solvent extraction. there is. This is because the extraction method of the present invention enables extraction, emulsification, and formation of fine double phase particles together, so that water-soluble and fat-soluble components, as well as components present in the cell membrane, are extracted from the extraction raw material, and each component is It is thought that this is because the network of these components can be exhibited by stably existing in a similar phase. Since the extract obtained by the extraction method of the present invention has such excellent physiological activity, it is expected to be useful in various fields such as medicine, bio, beauty and food.
도 1은 추출 원료에 존재하는 성분들의 상태와 본 발명 추출 방법에 따른 추출물에 존재하는 성분들의 상태를 예시적으로 나타낸 것이다.
도 2는 본 발명의 일실시예에 따른 추출물(실시예 1e)과 기존 추출 방법(단일 용매를 사용한 추출 방법)에 따른 추출물(비교예 1e 및 비교예 2e)의 HPLC 분석 결과를 나타낸 것이다.Figure 1 exemplarily shows the state of the components present in the extraction raw material and the state of the components present in the extract according to the extraction method of the present invention.
Figure 2 shows the HPLC analysis results of the extract (Example 1e) according to an embodiment of the present invention and the extract (Comparative Example 1e and Comparative Example 2e) according to the existing extraction method (extraction method using a single solvent).
본 발명의 추출 방법은 추출 원료에 인지질, 계면활성제, 인지질 이외의 지질, 수용성 용매 및 물을 가하고 교반함으로써, 추출 매개체의 유화(emulsification)가 이루어지면서 유용성분이 추출되도록 하여 추출 원료의 수용성 성분과 지용성 성분이 함께 추출될 수 있도록 하며, 또한 리포솜(liposome)과 같은 형태의 미세이중상 입자가 생성되도록 하여 예를 들어 세포나 세포소기관의 막(membrane)에 존재하는 성분도 효율적으로 추출될 수 있도록 하는 것을 특징으로 한다.In the extraction method of the present invention, phospholipids, surfactants, lipids other than phospholipids, water-soluble solvents and water are added to the extraction raw material and stirred, so that the useful components are extracted while emulsifying the extraction medium, so that the water-soluble component and the fat-soluble component of the extraction raw material It allows the components to be extracted together, and also to generate micro-dual-phase particles in the form of liposomes so that, for example, components present in the membrane of cells or organelles can be efficiently extracted. do it with
이러한 본 발명의 추출 방법에 따르면, 추출 원료의 수용성 성분과 지용성 성분 그리고 막에 존재하는 성분들을 함께 추출할 수 있을 뿐만 아니라, 이들 성분이 추출 조성물(추출로 얻어지는 조성물) 중에 안정적으로(예를 들어 수용성 성분은 에멀션 상태의 추출 조성물에서 수상부에, 지용성 성분은 유상부에, 막에 존재하는 성분은 미세이중상 입자의 인지질 이중층 사이에) 존재하도록 할 수 있다.According to the extraction method of the present invention, it is possible to extract the water-soluble component, the fat-soluble component of the extraction raw material, and the components present in the membrane together, and these components are stably (for example, The water-soluble component may be present in the aqueous phase of the extract composition in the emulsion state, the oil-soluble component may be present in the oil phase, and the component present in the membrane (between the phospholipid bilayers of the microbiphasic particles).
이에 생리활성(예를 들어 항산화 효과)의 발휘와 관련된 수용성 성분, 지용성 성분 및 막 성분이 추출 조성물 내에 안정적으로 존재할 수 있게 되므로, 이들 성분들의 네트워크를 통해 생체 내에서 발휘되는 것과 유사한 우수한 생리활성이 추출 조성물을 통해 발휘될 수 있게 된다.Accordingly, water-soluble components, fat-soluble components, and membrane components related to the exertion of physiological activity (for example, antioxidant effect) can be stably present in the extract composition, so excellent physiological activity similar to that exhibited in vivo through the network of these components is achieved. It can be exerted through the extract composition.
본 발명의 추출 방법은 상기와 같은 미세이중상 입자가 생성되도록 하여 생체에서 세포막, 세포막에 둘러 쌓인 세포질 및 세포 외부로 이루어지는 것과 유사한 환경을 제공하며, 각 부위(세포막, 세포질, 세포 외부)에 존재하던 유용성분을 추출 조성물 중에서도 유사한 환경 하에 위치시킬 수 있다는 점에서 생체 내 물질 간 네트워크가 반영될 수 있는 추출 방법이라고 할 수 있다.The extraction method of the present invention provides an environment similar to that of the cell membrane, the cytoplasm surrounded by the cell membrane, and the outside of the cell in a living body by generating the fine double-phase particles as described above, and is present in each site (cell membrane, cytoplasm, outside the cell). It can be said that it is an extraction method that can reflect the network between substances in a living body in that useful components can be located in a similar environment in the extraction composition.
이와 같이 본 발명의 추출 방법은 기존의 일반적인 추출 방법, 예를 들어 단일 용매 또는 단일계의 혼합용매를 사용하여 특정 성분만을 주로 추출하기 위한 방법들과 비교하여 그 구성이나 효과에 있어서 큰 차이가 있다.As such, the extraction method of the present invention has a large difference in composition or effect compared to conventional extraction methods, for example, methods for mainly extracting only a specific component using a single solvent or a single solvent mixture. .
또한, 본 발명의 추출 방법은 기존의 리포솜 제조기술 또는 나노에멀션 제조기술을 응용한다는 면에서 기존의 리포솜 또는 나노에멀션에 유용성분을 포함시키는 기술과 유사성이 있으나, 기존의 기술은 추출 원료가 아닌 추출물에 에멀션을 위한 성분을 가하고 교반하여 이미 추출된 성분을 리포솜 또는 나노에멀션 입자에 단순히 포집함으로써 생체 이용성을 높이는 기술인 것인 반면, 본 발명의 추출 방법은 추출물이 아닌 추출 원료에 에멀션을 위한 성분을 가하며 인지질을 사용함으로써 추출과 유화, 미세이중상 입자 형성이 함께 이루어지도록 한다는 점에서 차이가 있으며 이를 통해 상기와 같은 효과를 발휘할 수 있도록 한다는 점에서 기술적인 진보성이 있다.In addition, the extraction method of the present invention is similar to the existing technology for including useful ingredients in liposomes or nanoemulsions in terms of applying the existing liposome manufacturing technology or nanoemulsion manufacturing technology, but the existing technology is an extract that is not an extraction raw material While it is a technique to increase bioavailability by simply collecting the already extracted ingredients in liposomes or nanoemulsion particles by adding and stirring the ingredients for the emulsion to the emulsion, the extraction method of the present invention adds the ingredients for the emulsion to the extraction raw material, not the extract, There is a difference in that extraction, emulsification, and formation of fine double-phase particles are performed together by using phospholipids, and there is technological progress in that it enables the above effects to be exerted.
본 발명의 추출 방법에서 추출 원료에 가하는 인지질, 계면활성제, 인지질 이외의 지질, 수용성 용매 및 물은 추출 용매와 같은 추출 매개체의 역할을 하는 것으로, 이들의 교반을 통해 유화가 이루어지도록 하면서, 세포막 구조와 유사한 인지질 이중층의 입자가 형성되도록 할 수 있다.In the extraction method of the present invention, phospholipids, surfactants, lipids other than phospholipids, water-soluble solvents, and water added to the extraction raw material serve as extraction media such as extraction solvents, and while allowing emulsification through their stirring, cell membrane structure It is possible to form particles of a phospholipid bilayer similar to
본 발명의 추출 방법에서 추출 원료는 식물, 동물 또는 미생물 유래의 생물학적 원료일 수 있다. 예를 들어, 식물의 경우 과일(과육과 과일껍질을 함께 또는 별도로), 꽃, 잎, 뿌리, 줄기, 수피 등 식물의 일부분, 동물(인간을 제외한)의 경우 피부, 가죽, 내장, 혈액, 배설물, 타액 등 동물의 일부분, 미생물의 경우 세포, 세포군집, 포자 등일 수 있으며, 상기 식물 및 동물 유래의 생물학적 원료는 분리되거나 배양된 조직 또는 세포일 수도 있다.In the extraction method of the present invention, the extraction raw material may be a biological raw material derived from plants, animals or microorganisms. For example, in the case of plants, fruits (the flesh and the peel together or separately), flowers, leaves, roots, stems, parts of plants, such as bark, etc. In the case of animals (except humans), skin, hide, intestines, blood, excreta , parts of animals such as saliva, and in the case of microorganisms, cells, cell populations, spores, etc., and the biological raw materials derived from plants and animals may be isolated or cultured tissues or cells.
본 발명의 추출 방법에서 인지질은 바람직하게는 포스파티딜콜린(phosphatidylcholine), 포스파티딜세린(phosphatidylserine), 포스파티딜에탄올아민(phosphatidylethanolamine), 포스파티딜이노시톨(phosphatidylinositol), 레시틴(lecithin) 및 이들의 수소첨가물로 이루어진 군으로부터 선택된 하나 이상이다.In the extraction method of the present invention, the phospholipid is preferably selected from the group consisting of phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylinositol, lecithin, and hydrogenated products thereof. More than that.
본 발명의 추출 방법에서 계면활성제는 바람직하게는 스팬(span)형 계면활성제, 트윈(tween)형 계면활성제 및 비이온성 계면활성제로 이루어진 군으로부터 선택되는 하나 이상이다. 보다 구체적으로 상기 스팬(span)형 계면활성제는 바람직하게는 소르비탄 라우레이트(sorbitan laurate), 소르비탄 팔미테이트(sorbitan palmirate), 소르비탄 스테아레이트(sorbitan stearate) 및 소르비탄 올레이트(sorbitan oleate)로 이루어진 군으로부터 선택할 수 있으며, 상기 트윈(tween)형 계면활성제는 바람직하게는 폴리소르베이트(polysorbate), 폴리소르베이트 20(polysorbate 20), 폴리소르베이트 60(polysorbate 60) 및 폴리소르베이트 80(polysorbate 80)으로 이루어진 군으로부터 선택할 수 있으며, 상기 비이온성 계면활성제는 바람직하게는 PEG-8 카프릴릭/카프릭 글리세라이드(PEG-8 caprylic/capric glycerides), 폴록사머 188(poloxamer 188) 및 폴록사머 407(poloxamer 407)로 이루어진 군으로부터 선택할 수 있다.In the extraction method of the present invention, the surfactant is preferably at least one selected from the group consisting of span-type surfactants, twin-type surfactants and nonionic surfactants. More specifically, the span type surfactant is preferably sorbitan laurate, sorbitan palmirate, sorbitan stearate and sorbitan oleate. It can be selected from the group consisting of, and the tween surfactant is preferably polysorbate, polysorbate 20, polysorbate 60, and polysorbate 80 ( polysorbate 80), and the nonionic surfactant is preferably PEG-8 caprylic/capric glycerides, poloxamer 188 and poloxamer 188. It can be selected from the group consisting of poloxamer 407.
본 발명의 추출 방법에서 인지질 이외의 지질은 바람직하게는 탄소수 12 이상의 지질이다. 보다 구체적으로 바람직하게는 지방산 에스테르 및 식물성 오일로 이루어진 군으로부터 선택되는 하나 이상이다. 보다 구체적으로 상기 지방산 에스테르는 바람직하게는 세틸 카프레이트(cetyl caprate), 세틸 팔미테이트(cetyl palmitate) 및 세틸 에틸헥사노에이트(cetyl ethylhexanoate)로 이루어지는 군으로부터 선택할 수 있으며, 상기 식물성 오일은 바람직하게는 아르간 오일(argan oil), 아보카도 오일(avocado oil), 포도씨유(grape seed oil), 마카다미아 너트유(macadamia nut oil) 및 올리브유(olive oil)로 이루어진 군으로부터 선택할 수 있다.In the extraction method of the present invention, lipids other than phospholipids are preferably lipids having 12 or more carbon atoms. More specifically, it is preferably at least one selected from the group consisting of fatty acid esters and vegetable oils. More specifically, the fatty acid ester may be selected from the group consisting of cetyl caprate, cetyl palmitate and cetyl ethylhexanoate, and the vegetable oil is preferably It may be selected from the group consisting of argan oil, avocado oil, grape seed oil, macadamia nut oil and olive oil.
본 발명의 추출 방법에서 수용성 용매는 바람직하게는 탄소수 2 내지 6의 알코올이다. 보다 구체적으로 바람직하게는 에탄올(ethyl alcohol), 프로판올(propyl alcohol), 이소프로판올(isopropyl alcohol), 프로필렌 글라이콜(propylene glycol), 이소프로필렌 글라이콜(isopropylene glycol), 디플로필렌 글라이콜(dipropylene glycol), 부틸렌 글라이콜(butylene glycol), 에톡시디글라이콜(ethoxydiglycol), 헥산디올(hexanediol), 글리세린(glycerin) 및 메틸피롤리돈(methylpyrrolidone)으로 이루어진 군으로부터 선택되는 하나 이상이다.In the extraction method of the present invention, the water-soluble solvent is preferably an alcohol having 2 to 6 carbon atoms. More specifically, preferably ethanol (ethyl alcohol), propanol (propyl alcohol), isopropanol (isopropyl alcohol), propylene glycol (propylene glycol), isopropylene glycol (isopropylene glycol), dipropylene glycol ( dipropylene glycol), butylene glycol, ethoxydiglycol, hexanediol, glycerin, and methylpyrrolidone is at least one selected from the group consisting of .
본 발명의 추출 방법에서 추출 원료에 인지질, 계면활성제, 인지질 이외의 지질, 수용성 용매 및 물을 가하는 것은 바람직하게는 1시간 이내에 이루어지도록 한다. 즉, 추출 원료에 상기 5가지의 추출 매개체 성분(인지질, 계면활성제, 인지질 이외의 지질, 수용성 용매 및 물) 모두를 가하는데 걸리는 시간을 1시간 이내로 하는 것이 바람직하다. 예를 들어, 상기 5가지의 추출 매개체 성분을 각각 순서대로 가하는 경우, 첫 번째 투입하는 추출 매개체 성분의 투입을 시작하는 시점부터 마지막 다섯 번째 투입하는 추출 매개체 성분의 투입이 끝나는 시점까지의 시간을 1시간 이내로 하는 것이 바람직하다. 보다 바람직하게는 이 시간을 30분 이내로 하며, 더욱 바람직하게는 20분, 더욱 바람직하게는 15분, 더욱 바람직하게는 10분, 더욱 바람직하게는 5분, 더욱 바람직하게는 1분 이내로 하며, 더욱 바람직하게는 동시에 가한다.In the extraction method of the present invention, the addition of phospholipids, surfactants, lipids other than phospholipids, water-soluble solvents and water to the extraction raw material is preferably performed within 1 hour. That is, it is preferable that the time it takes to add all of the five extraction medium components (phospholipids, surfactants, lipids other than phospholipids, water-soluble solvents and water) to the extraction raw material is less than 1 hour. For example, when each of the five extraction medium components is added in order, the time from the start of the first input of the extraction medium component to the end of the fifth input of the extraction medium component is 1 It is preferable to do it within an hour. More preferably, this time is set to 30 minutes or less, more preferably 20 minutes, more preferably 15 minutes, still more preferably 10 minutes, still more preferably 5 minutes, still more preferably 1 minute or less, further Preferably at the same time.
본 발명의 추출 방법에서 인지질, 계면활성제, 인지질 이외의 지질, 수용성 용매 및 물의 양은 바람직하게는 상기 5가지의 추출 매개체 성분을 모두 합한 중량(총 추출 매개체 성분의 양)을 기준으로 인지질 0.1 내지 30중량%, 계면활성제 0.1 내지 30중량%, 인지질 이외의 지질 0.1 내지 70중량%, 수용성 용매 0.1 내지 70중량%, 나머지는 물로 한다. 보다 바람직하게는 상기 5가지의 추출 매개체 성분을 모두 합한 중량을 기준으로 인지질 0.5 내지 20중량%, 계면활성제 2 내지 30중량%, 인지질 이외의 지질 0.1 내지 20중량%, 수용성 용매 15 내지 50중량%, 나머지는 물로 한다. 보다 바람직하게는 상기 5가지의 추출 매개체 성분을 모두 합한 중량을 기준으로 인지질 2 내지 6중량%, 계면활성제 3 내지 20중량%, 인지질 이외의 지질 1 내지 5중량%, 수용성 용매 15 내지 40중량%, 나머지는 물로 한다.In the extraction method of the present invention, the amounts of phospholipids, surfactants, lipids other than phospholipids, water-soluble solvents and water are preferably 0.1 to 30 of phospholipids based on the combined weight of all five extraction medium components (the amount of total extraction medium components). % by weight, surfactant 0.1 to 30% by weight, lipids other than phospholipids 0.1 to 70% by weight, water-soluble solvent 0.1 to 70% by weight, the remainder being water. More preferably, based on the total weight of the five extraction medium components, 0.5 to 20% by weight of phospholipids, 2 to 30% by weight of surfactant, 0.1 to 20% by weight of lipids other than phospholipids, 15 to 50% by weight of water-soluble solvent , and do the rest with water. More preferably, based on the total weight of the five extraction medium components, 2 to 6% by weight of phospholipids, 3 to 20% by weight of surfactant, 1 to 5% by weight of lipids other than phospholipids, 15 to 40% by weight of water-soluble solvent , and do the rest with water.
본 발명의 추출 방법에서 인지질, 계면활성제, 인지질 이외의 지질, 수용성 용매 및 물을 유상부와 수상부로 구분하여 별도로 준비한 다음 이들을 추출 원료에 가하는 방식을 사용할 수 있다. 예를 들어, 인지질, 계면활성제 및 인지질 이외의 지질을 혼합하여 유상부를 준비하고, 수용성 용매 및 물을 혼합하여 수상부를 준비한 다음, 추출 원료에 상기 유상부 및 수상부를 가하는 방식을 사용할 수 있다. 이에 따르면 상기와 같은 본 발명의 추출 방법에 따른 기대 효과를 높일 수 있다.In the extraction method of the present invention, a method in which phospholipids, surfactants, lipids other than phospholipids, water-soluble solvents and water are divided into an oil phase and an aqueous phase and separately prepared, and then added to the extraction raw material may be used. For example, a method of preparing an oil phase by mixing phospholipids, surfactants and lipids other than phospholipids, preparing an aqueous phase by mixing a water-soluble solvent and water, and then adding the oil phase and aqueous phase to the extraction raw material may be used. According to this, it is possible to increase the expected effect according to the extraction method of the present invention as described above.
이때 추출 원료에 상기 유상부 및 수상부를 가하는 것은 바람직하게는 1시간 이내에 이루어지도록 한다. 즉, 추출 원료에 상기 유상부 및 수상부 모두를 가하는데 걸리는 시간을 1시간 이내로 하는 것이 바람직하다. 예를 들어, 상기 유상부 및 수상부를 순서대로 가하는 경우, 먼저 투입하는 수상부(또는 유상부)의 투입을 시작하는 시점부터 다음 유상부(또는 수상부)의 투입이 끝나는 시점까지의 시간을 1시간 이내로 하는 것이 바람직하다. 보다 바람직하게는 이 시간을 30분 이내로 하며, 더욱 바람직하게는 20분, 더욱 바람직하게는 15분, 더욱 바람직하게는 10분, 더욱 바람직하게는 5분, 더욱 바람직하게는 1분 이내로 하며, 더욱 바람직하게는 동시에 가한다.At this time, the addition of the oil phase part and the aqueous phase part to the extraction raw material is preferably made within 1 hour. That is, it is preferable that the time it takes to add both the oil phase part and the aqueous phase part to the extraction raw material is less than 1 hour. For example, when the oil phase part and the water phase part are added in order, the time from the start of the input of the first water phase part (or the oil phase part) to the point where the input of the next oil phase part (or the water phase part) ends is less than 1 hour it is preferable More preferably, this time is set to 30 minutes or less, more preferably 20 minutes, more preferably 15 minutes, still more preferably 10 minutes, still more preferably 5 minutes, still more preferably 1 minute or less, further Preferably at the same time.
본 발명의 추출 방법에서 추출 원료와 추출 매개체의 비율은 바람직하게는 추출 원료 1중량부를 기준으로 추출 매개체(상기 5가지의 추출 매개체 성분을 모두 합한 중량 기준) 1 내지 1000중량부로 하며, 보다 바람직하게는 2 내지 100중량부로 하고, 보다 바람직하게는 5 내지 50중량부로 한다.In the extraction method of the present invention, the ratio of the extraction material and the extraction medium is preferably 1 to 1000 parts by weight of the extraction medium (based on the weight of the sum of the five extraction medium components) based on 1 part by weight of the extraction material, more preferably is 2 to 100 parts by weight, more preferably 5 to 50 parts by weight.
본 발명의 추출 방법에서 교반은 교반기를 사용하여 수행할 수 있다. 교반기의 종류는 특별히 제한되지 않으며, 예를 들어 일반적인 교반기, 이중나선 교반기, 고속 유화기, 균질기, 혼합기 또는 초음파 균질기 등을 사용할 수 있다.In the extraction method of the present invention, stirring may be performed using a stirrer. The type of the stirrer is not particularly limited, and for example, a general stirrer, a double helix stirrer, a high-speed emulsifier, a homogenizer, a mixer, or an ultrasonic homogenizer may be used.
본 발명의 추출 방법에서 교반 시 적용하는 온도는 바람직하게는 혼합물, 즉 추출 원료와 인지질, 계면활성제, 인지질 이외의 지질, 수용성 용매 및 물의 혼합물의 중심 온도를 기준으로 10 내지 80℃로 하며, 보다 바람직하게는 20 내지 80℃, 보다 바람직하게는 20 내지 60℃로 한다. 교반 시간은 바람직하게는 10분 내지 30일로 하며, 보다 바람직하게는 30분 내지 72시간으로 한다. 교반체를 사용하거나 진탕기를 사용하는 등 회전하는 방식으로 교반하는 경우, 교반 분당 회전수(rpm)는 10 내지 3000rpm, 보다 바람직하게는 50 내지 1500rpm으로 한다.The temperature applied during stirring in the extraction method of the present invention is preferably 10 to 80 ° C. based on the central temperature of the mixture, that is, the mixture of the extraction raw material, phospholipids, surfactants, lipids other than phospholipids, water-soluble solvents, and water, and more Preferably it is 20-80 degreeC, More preferably, it is set as 20-60 degreeC. The stirring time is preferably 10 minutes to 30 days, more preferably 30 minutes to 72 hours. When stirring in a rotating manner such as using a stirring body or a shaker, the number of rotations per minute (rpm) of stirring is 10 to 3000 rpm, more preferably 50 to 1500 rpm.
본 발명의 추출 방법에서 상기 교반하는 단계 이후 추출 잔사를 제거하는 단계를 더 포함할 수 있다.In the extraction method of the present invention, the method may further include removing the extraction residue after the stirring.
추출 잔사를 제거하는 단계는 상기 교반을 통해 추출 및 유화가 충분히 이루어진 다음 원심분리, 여과 등의 방법을 사용하여 수행할 수 있다.The step of removing the extraction residue may be performed using a method such as centrifugation or filtration after extraction and emulsification are sufficiently performed through the stirring.
상기와 같은 본 발명의 추출 방법은 항산화, 항노화, 항염증, 피부미백, 피부주름개선, 항암, 항당뇨, 항박테리아, 항바이러스 등의 다양한 생리활성 중에서 하나 이상의 특정 생리활성을 나타내는 추출물을 얻기 위한 용도로 사용될 수 있다. 특히, 본 발명에 따르면 버섯류, 식물류 및 조류(algae)로 이루어진 군으로부터 선택된 하나 이상의 추출 원료로부터 우수한 항산화 활성을 갖는 추출물을 얻는데 유용하다.The extraction method of the present invention as described above obtains an extract exhibiting at least one specific physiological activity among various physiological activities such as antioxidant, anti-aging, anti-inflammatory, skin whitening, skin wrinkle improvement, anti-cancer, anti-diabetic, anti-bacterial, anti-viral, etc. can be used for In particular, according to the present invention, it is useful to obtain an extract having excellent antioxidant activity from one or more extraction raw materials selected from the group consisting of mushrooms, plants and algae.
상기와 같은 본 발명의 추출 방법으로 추출된 추출물은 생물 원료의 다양한 유용성분들, 예를 들어 세포막에서 발견되는 지용성 물질들, 세포의 수용성 부분들로부터 나온 극성 물질들, 산성/염기성 이온들, 안정한 구조물과 산화된 구조물 등을 복합적으로 함유할 수 있으며, 이들 성분들을 생체 내에서와 유사한 환경 하에 존재하는 형태로 안정적으로 함유할 수 있어, 다양한 유용성분들 간의 네트워크를 통해 우수한 생리활성을 나타낼 수 있다.The extract extracted by the extraction method of the present invention as described above includes various useful components of biological raw materials, for example, fat-soluble substances found in cell membranes, polar substances from water-soluble parts of cells, acidic/basic ions, stable structures It can contain a complex of peroxidized structures, and these components can be stably contained in a form that exists under an environment similar to in vivo, so that excellent physiological activity can be exhibited through a network between various useful components.
따라서 본 발명의 추출 방법으로 추출된 추출물은 그 자체로도 화장료나 식품, 특히 기능성 화장료나 기능성 식품으로 이용할 수 있다.Therefore, the extract extracted by the extraction method of the present invention can be used as a cosmetic or food, in particular, a functional cosmetic or functional food.
본 발명의 추출물은 또한 다른 물질들과 혼합된 조성물로 제공될 수 있다. 예를 들어, 화장품학적 또는 식품학적으로 허용되는 물질들과 혼합된 형태로 제형화된 화장료 조성물 또는 식품 조성물의 형태로 제공될 수 있다.The extract of the present invention may also be provided in a composition admixed with other substances. For example, it may be provided in the form of a cosmetic composition or a food composition formulated in a form mixed with cosmetically or foodologically acceptable substances.
이때 조성물 중 상기 추출물의 함량에 대한 특별한 제한은 없으며, 예를 들어 조성물 총 중량의 0.01 내지 99%일 수 있다.At this time, there is no particular limitation on the content of the extract in the composition, for example, may be 0.01 to 99% of the total weight of the composition.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .
[실시예][Example]
추출 원료로 사용할 상황버섯, 아로니아 열매, 레몬밤잎 및 복분자는 각각 파쇄하여 준비하고, 스피룰리나는 건조원말을 그대로 이용하였다.Sanghwang mushroom, aronia fruit, lemon balm leaf and bokbunja to be used as extraction raw materials were prepared by crushing each, and dried raw spirulina was used as it is.
인지질로 레시틴을 사용하였고, 계면활성제로 PEG-8 카프릴릭/카프릭 글리세라이드(PEG-8 caprylic/capric glycerides) 및 폴록사머 407(poloxamer 407)을 사용하였고, 인지질 이외의 지질로 세틸 에틸헥사노에이트(cetyl ethylhexanoate)를 사용하였고, 수용성 용매로 부틸렌 글라이콜(butylene glycol) 및 헥산디올(hexanediol)을 사용하였다.Lecithin was used as a phospholipid, PEG-8 caprylic/capric glycerides and poloxamer 407 were used as surfactants, and cetyl ethylhexa Noate (cetyl ethylhexanoate) was used, and butylene glycol (butylene glycol) and hexanediol (hexanediol) were used as water-soluble solvents.
유상부 준비 : 레시틴, PEG-8 카프릴릭/카프릭 글리세라이드, 폴록사머 407, 세틸 에틸헥사노에이트, 부틸렌 글라이콜 및 물을 모두 합한 중량을 기준으로, 레시틴 5중량%, PEG-8 카프릴릭/카프릭 글리세라이드 5중량%, 폴록사머 407 1중량% 및 세틸 에틸헥사노에이트 3중량%를 혼합하고 80℃에서 교반하여 유상부를 제조하였다.Preparation of the oil phase: Lecithin 5% by weight, PEG-8 based on the combined weight of lecithin, PEG-8 caprylic/capric glyceride, poloxamer 407, cetyl ethylhexanoate, butylene glycol and water An oil phase was prepared by mixing 5% by weight of caprylic/capric glyceride, 1% by weight of poloxamer 407 and 3% by weight of cetyl ethylhexanoate and stirring at 80°C.
수상부 준비 : 레시틴, PEG-8 카프릴릭/카프릭 글리세라이드, 폴록사머 407, 세틸 에틸헥사노에이트, 부틸렌 글라이콜 및 물을 모두 합한 중량을 기준으로, 부틸렌 글라이콜 30중량%, 헥산디올 2.5중량% 및 정제수 53.5중량%를 혼합하고 30℃에서 교반하여 수상부를 제조하였다.Preparation of aqueous phase: 30 weight of butylene glycol based on the combined weight of lecithin, PEG-8 caprylic/capric glyceride, poloxamer 407, cetyl ethylhexanoate, butylene glycol and water %, 2.5% by weight of hexanediol and 53.5% by weight of purified water were mixed and stirred at 30° C. to prepare an aqueous phase.
실시예 1. 유용성분의 추출Example 1. Extraction of useful ingredients
실시예 1a. 상황버섯 추출물 제조Example la. Manufacture of Sanghwang mushroom extract
상황버섯 파쇄물 5g을 용기에 담고 상기 수상부 86g 및 유상부 14g을 동시에 첨가하면서 교반하였다. 교반은 상온에서 500rpm으로 수행하였으며, 수상부 및 유상부를 첨가하면서 교반하기 시작하여 24시간 동안 교반하였다. 교반 이후 생성된 추출혼합물을 4,000rpm으로 원심분리하고 0.2㎛ 기공의 막 필터로 여과하여 잔사가 제거된 추출물을 수득하였다.5 g of shredded mushrooms of the Sangha mushroom were placed in a container and stirred while simultaneously adding 86 g of the aqueous phase and 14 g of the oil phase. Stirring was performed at 500 rpm at room temperature, and stirring was started while adding an aqueous phase and an oil phase, followed by stirring for 24 hours. After stirring, the resulting extract mixture was centrifuged at 4,000 rpm and filtered through a 0.2 μm pore membrane filter to obtain an extract from which residues were removed.
실시예 1b. 아로니아 추출물 제조Example 1b. Aronia Extract Preparation
추출 대상으로 상황버섯 파쇄물 대신 아로니아 열매 파쇄물을 적용한 것을 제외하고는 상기 실시예 1a와 동일하게 수행하였다.It was carried out in the same manner as in Example 1a, except that the aronia fruit lysate was applied instead of the chrysanthemum mushroom lysate as an extraction target.
실시예 1c. 레몬밤잎 추출물 제조Example 1c. Preparation of lemon balm leaf extract
추출 대상으로 상황버섯 파쇄물 대신 레몬밤잎 파쇄물을 적용한 것을 제외하고는 상기 실시예 1a와 동일하게 수행하였다.It was carried out in the same manner as in Example 1a, except that the lemon balm leaf lysate was applied as an extraction target instead of the shredded sanghaeng mushroom.
실시예 1d. 스피룰리나 추출물 제조Example 1d. Spirulina Extract Preparation
추출 대상으로 상황버섯 파쇄물 대신 스피룰리나 건조원말을 적용한 것을 제외하고는 상기 실시예 1a와 동일하게 수행하였다.It was carried out in the same manner as in Example 1a, except that spirulina dried raw powder was applied instead of the shredded Sanghwang mushroom as an extraction target.
실시예 1e. 복분자 추출물 제조Example 1e. Preparation of bokbunja extract
추출 대상으로 상황버섯 파쇄물 대신 복분자 파쇄물을 적용한 것을 제외하고는 상기 실시예 1a와 동일하게 수행하였다.It was carried out in the same manner as in Example 1a, except that bokbunja lysate was applied as an extraction target instead of mulberry lysate.
비교예 1. 물 추출물 제조Comparative Example 1. Preparation of water extract
비교예 1a. 상황버섯 물 추출물 제조Comparative Example 1a. Manufacture of Sanghwang mushroom water extract
상황버섯 파쇄물 5g을 용기에 담고 헥산디올 2.5%를 포함하는 1차 정제수 100㎖를 가하고 상온에서 500rpm으로 24시간 동안 교반하면서 추출한 다음, 4000rpm으로 원심분리하고 0.2㎛ 기공의 막 필터로 여과하여 잔사가 제거된 물 추출물을 수득하였다.Put 5 g of shredded mushrooms in a container, add 100 ml of primary purified water containing 2.5% of hexanediol, and extract while stirring at 500 rpm at room temperature for 24 hours, centrifuge at 4000 rpm, and filter with a 0.2 μm pore membrane filter to remove the residue. The removed water extract was obtained.
비교예 1b. 아로니아 물 추출물 제조Comparative Example 1b. Aronia Water Extract Preparation
추출 대상으로 상황버섯 파쇄물 대신 아로니아 열매 파쇄물을 적용한 것을 제외하고는 상기 비교예 1a와 동일하게 수행하였다.It was carried out in the same manner as in Comparative Example 1a, except that the crushed Aronia fruit was applied as an extraction target instead of the crushed Sangha mushroom.
비교예 1c. 레몬밤잎 물 추출물 제조Comparative Example 1c. Preparation of lemon balm leaf water extract
추출 대상으로 상황버섯 파쇄물 대신 레몬밤잎 파쇄물을 적용한 것을 제외하고는 상기 비교예 1a와 동일하게 수행하였다.It was carried out in the same manner as in Comparative Example 1a, except that the lemon balm leaf lysate was applied as an extraction target instead of the shredded Sanghwang mushroom.
비교예 1d. 스피룰리나 물 추출물 제조Comparative Example 1d. Preparation of Spirulina Water Extract
추출 대상으로 상황버섯 파쇄물 대신 스피룰리나 건조원말을 적용한 것을 제외하고는 상기 비교예 1a와 동일하게 수행하였다.It was carried out in the same manner as in Comparative Example 1a, except that spirulina dried raw powder was applied as an extraction target instead of shredded mushrooms.
비교예 1e. 복분자 물 추출물 제조Comparative Example 1e. Preparation of bokbunja water extract
추출 대상으로 상황버섯 파쇄물 대신 복분자 파쇄물을 적용한 것을 제외하고는 상기 비교예 1a와 동일하게 수행하였다.It was carried out in the same manner as in Comparative Example 1a, except that bokbunja lysate was applied as an extraction target instead of mulberry lysate.
비교예 2. 에탄올 추출물 제조Comparative Example 2. Preparation of ethanol extract
비교예 2a. 상황버섯 에탄올 추출물 제조Comparative Example 2a. Manufacture of Sanghwang mushroom ethanol extract
상황버섯 파쇄물 5g을 용기에 담고 에탄올 100㎖를 가하고 상온에서 500rpm으로 24시간 동안 교반하면서 추출한 다음, 4000rpm으로 원심분리하고 0.2㎛ 기공의 막 필터로 여과하여 잔사가 제거된 에탄올 추출물을 수득하였다.5 g of lysate lysate was placed in a container, 100 ml of ethanol was added, and extracted while stirring at 500 rpm at room temperature for 24 hours, then centrifuged at 4000 rpm and filtered through a 0.2 μm pore membrane filter to obtain an ethanol extract from which residues were removed.
비교예 2b. 아로니아 에탄올 추출물 제조Comparative Example 2b. Aronia Ethanol Extract Preparation
추출 대상으로 상황버섯 파쇄물 대신 아로니아 열매 파쇄물을 적용한 것을 제외하고는 상기 비교예 2a와 동일하게 수행하였다.It was carried out in the same manner as in Comparative Example 2a, except that the crushed Aronia fruit was applied as an extraction target instead of the crushed Sangha mushroom.
비교예 2c. 레몬밤잎 에탄올 추출물 제조Comparative Example 2c. Preparation of lemon balm leaf ethanol extract
추출 대상으로 상황버섯 파쇄물 대신 레몬밤잎 파쇄물을 적용한 것을 제외하고는 상기 비교예 2a와 동일하게 수행하였다.It was carried out in the same manner as in Comparative Example 2a, except that the lemon balm leaf lysate was applied as an extraction target instead of the shredded Sanghwang mushroom.
비교예 2d. 스피룰리나 에탄올 추출물 제조Comparative Example 2d. Preparation of spirulina ethanol extract
추출 대상으로 상황버섯 파쇄물 대신 스피룰리나 건조원말을 적용한 것을 제외하고는 상기 비교예 2a와 동일하게 수행하였다.It was carried out in the same manner as in Comparative Example 2a, except that spirulina dried raw powder was applied as an extraction target instead of shredded mushrooms.
비교예 2e. 복분자 에탄올 추출물 제조Comparative Example 2e. Bokbunja ethanol extract production
추출 대상으로 상황버섯 파쇄물 대신 복분자 파쇄물을 적용한 것을 제외하고는 상기 비교예 2a와 동일하게 수행하였다.It was carried out in the same manner as in Comparative Example 2a, except that bokbunja lysate was applied as an extraction target instead of mulberry lysate.
비교예 3. 물 추출물 포집 베지클 함유 유화액 제조Comparative Example 3. Preparation of emulsion containing water extract collecting vesicles
상기 비교예 1a의 상황버섯 물 추출물(상황버섯 5g을 사용하여 수득한 추출물 전부)을 본래 추출물 중량의 약 40%가 되도록 농축하였다. 이후 이 농축물 전부가 상기 수상부에서 헥산디올 대신 정제수로 대체한 경우의 정제수 부분에 포함되도록 부틸렌 글라이콜 및 정제수를 첨가, 즉 농축물 전부, 부틸렌 글라이콜 및 추가로 첨가하는 정제수를 합한 무게가 86g이 되도록 맞춘 다음, 여기에 유상부 14g을 첨가하면서 교반하였다. 교반은 상온에서 500rpm으로 수행하였으며, 수상부 및 유상부를 첨가하면서 교반하기 시작하여 24시간 동안 교반하였다. 교반 이후 생성된 추출혼합물을 4,000rpm으로 원심분리하고 0.2㎛ 기공의 막 필터로 여과하여 비교예 1a의 상황버섯 물 추출물이 단순 포집된 베지클(vesicle) 함유 유화액을 수득하였다.The Sanghwang mushroom water extract of Comparative Example 1a (all extracts obtained using 5 g of Sanghwang mushroom) was concentrated to about 40% of the original extract weight. Thereafter, butylene glycol and purified water are added so that all of this concentrate is included in the purified water part when purified water is replaced with hexanediol in the aqueous phase, that is, all of the concentrate, butylene glycol, and purified water to be additionally added The combined weight was adjusted to be 86 g, and then, 14 g of the oil phase was added thereto while stirring. Stirring was performed at 500 rpm at room temperature, and stirring was started while adding an aqueous phase and an oil phase, followed by stirring for 24 hours. After stirring, the resulting extract mixture was centrifuged at 4,000 rpm and filtered through a 0.2 μm pore membrane filter to obtain an emulsion containing vesicles in which the Sanghwang mushroom water extract of Comparative Example 1a was simply collected.
비교예 4. 에탄올 추출물 포집 베지클 함유 유화액 제조Comparative Example 4. Preparation of emulsion containing ethanol extract collecting vesicles
상기 비교예 2a의 상황버섯 에탄올 추출물(상황버섯 5g을 사용하여 수득한 추출물 전부)을 감압농축 분말화한 다음 용기에 담고 상기 수상부 86g 및 유상부 14g을 동시에 첨가하면서 교반하였다. 교반은 상온에서 500rpm으로 수행하였으며, 수상부 및 유상부를 첨가하면서 교반하기 시작하여 24시간 동안 교반하였다. 교반 이후 생성된 추출혼합물을 4,000rpm으로 원심분리하고 0.2㎛ 기공의 막 필터로 여과하여 비교예 2a의 상황버섯 에탄올 추출물이 단순 포집된 베지클(vesicle) 함유 유화액을 수득하였다.The ethanol extract of Sanghuang mushroom of Comparative Example 2a (all extracts obtained by using 5g of Sanghwang mushroom) was concentrated under reduced pressure and powdered, placed in a container, and stirred while simultaneously adding 86 g of the aqueous phase and 14 g of the oil phase. Stirring was performed at 500 rpm at room temperature, and stirring was started while adding an aqueous phase and an oil phase, followed by stirring for 24 hours. After stirring, the resulting extract mixture was centrifuged at 4,000 rpm and filtered through a 0.2 μm pore membrane filter to obtain an emulsion containing vesicles in which the ethanol extract of the Sangha mushroom of Comparative Example 2a was simply collected.
비교예 5. 물 추출물 및 에탄올 추출물 포집 베지클 함유 유화액 제조Comparative Example 5. Preparation of emulsion containing vesicles containing water extract and ethanol extract
상기 비교예 2a의 상황버섯 에탄올 추출물(상황버섯 5g을 사용하여 수득한 추출물 전부)을 감압농축 분말화한 다음 용기에 담고 상기 비교예 3의 수상부, 즉 비교예 1a의 상황버섯 물 추출물이 함유된 상태의 수상부(86g) 및 유상부 14g을 동시에 첨가하면서 교반하였다. 교반은 상온에서 500rpm으로 수행하였으며, 수상부 및 유상부를 첨가하면서 교반하기 시작하여 24시간 동안 교반하였다. 교반 이후 생성된 추출혼합물을 4,000rpm으로 원심분리하고 0.2㎛ 기공의 막 필터로 여과하여 비교예 1a의 상황버섯 물 추출물 및 비교예 2a의 상황버섯 에탄올 추출물이 단순 포집된 베지클(vesicle) 함유 유화액을 수득하였다.The Sanghwang mushroom ethanol extract of Comparative Example 2a (all extracts obtained using 5 g of Sanghwang mushroom) was concentrated and powdered under reduced pressure, put in a container, and the aqueous phase part of Comparative Example 3, that is, the Sanghwang mushroom water extract of Comparative Example 1a was contained. The aqueous phase (86 g) and 14 g of the oil phase were stirred while simultaneously adding them. Stirring was performed at 500 rpm at room temperature, and stirring was started while adding an aqueous phase and an oil phase, followed by stirring for 24 hours. After stirring, the resulting extract mixture was centrifuged at 4,000 rpm and filtered through a membrane filter with a 0.2 μm pore. was obtained.
실시예 2. 생리활성 확인Example 2. Confirmation of physiological activity
상기 실시예 1a 내지 1e, 비교예 1a 내지 1e, 비교예 2a 내지 2e, 비교예 3, 비교예 4 및 비교예 5의 추출물에 대한 항산화 효과를 확인하였다. 각 시료를 DMSO를 사용하여 0.16 내지 5%(w/w)의 농도가 되도록 희석하여 농도별 시료를 준비하였다.The antioxidant effect on the extracts of Examples 1a to 1e, Comparative Examples 1a to 1e, Comparative Examples 2a to 2e, Comparative Example 3, Comparative Example 4 and Comparative Example 5 was confirmed. Each sample was diluted to a concentration of 0.16 to 5% (w/w) using DMSO to prepare a sample for each concentration.
DPPH(2,2-diphenyl-1-picrylhydrazyl)는 화학적으로 안정화된 수용성 자유 라디칼(free radical)로서 방향족 화합물, 방향족 아민류에 의해 환원, 라디칼이 소거되어 기존 보라색에서 노란색으로 탈색된다. 즉, 환원력이 클수록 항산화 능력이 크다고 평가되며, 517㎚ 흡광도에서 그 효과를 단시간에 확인할 수 있는 장점이 있다. 이에 상기 실시예 및 비교예 추출물의 항산화 효과를 DPPH 라디칼 소거능을 흡광도로 측정하여 평가하였다.DPPH (2,2-diphenyl-1-picrylhydrazyl) is a chemically stabilized water-soluble free radical, which is reduced by aromatic compounds and aromatic amines and the radicals are removed, so that the existing purple to yellow color is discolored. That is, the greater the reducing power, the greater the antioxidant ability is evaluated, and there is an advantage in that the effect can be confirmed in a short time at the absorbance at 517 nm. Accordingly, the antioxidant effect of the extracts of Examples and Comparative Examples was evaluated by measuring the absorbance of the DPPH radical scavenging ability.
이를 위해, 상기 0.2mM 농도의 DPPH 용액 80㎕에 0.16 내지 5%(w/w) 농도의 시료 용액 20㎕를 첨가하여 30분간 실온에서 반응시킨 후 25% 에탄올 200㎕를 첨가한 후 517㎚에서 흡광도(SpectraMax i3, Molecular devices, CA, USA)를 측정하였다. 항산화 능력(scavenging activity)은 하기 수학식 1에 의하여 산출하였으며, 그 결과를 하기 표 1에 나타내었다.To this end, 20 μl of a sample solution of 0.16 to 5% (w/w) concentration was added to 80 μl of the 0.2 mM DPPH solution and reacted at room temperature for 30 minutes, after which 200 μl of 25% ethanol was added, and then at 517 nm. Absorbance (SpectraMax i3, Molecular devices, CA, USA) was measured. Antioxidant activity (scavenging activity) was calculated by Equation 1 below, and the results are shown in Table 1 below.
[수학식 1][Equation 1]
항산화능(%) = (1-시료처리군의 흡광도/시료무처리군의 흡광도)*100Antioxidant activity (%) = (1-absorbance in the sample treated group / absorbance in the untreated group) * 100
실험 결과, 시험된 모든 농도에서 비교예 1 내지 5에 비해서 실시예 1의 항산화 능력이 매우 우수함을 확인하였다.As a result of the experiment, it was confirmed that the antioxidant ability of Example 1 was very excellent compared to Comparative Examples 1 to 5 at all concentrations tested.
실시예 3. HPLC 분석Example 3. HPLC analysis
상기 실시예 1e, 비교예 1e 및 비교예 2e 각 추출물을 HPLC로 분석하였다.Each extract of Example 1e, Comparative Example 1e and Comparative Example 2e was analyzed by HPLC.
HPLC는 C18 컬럼을 이용하고 이동상 A(0.1% phosphoric acid)와 이동상 B(methanol)를 70 : 30에서부터 30 : 70까지의 구배(gradient)로 적용하며, UV 280nm에서 검출하는 방법을 사용하였다.For HPLC, a C18 column was used, and mobile phase A (0.1% phosphoric acid) and mobile phase B (methanol) were applied in a gradient from 70:30 to 30:70, and detection at UV 280nm was used.
동등한 조건에서 각 추출물을 HPLC로 분석한 결과, 도 2에서와 같이, 실시예 1e의 피크(peak)가 비교예 1e 및 비교예 2e의 피크를 모두 포함할 뿐만 아니라 함량도 높은 것으로 나타났다. 이는 본 발명의 추출 방법에 따르면 수용성 및 유용성 효능 성분을 모두 추출할 수 있으며 그 효율 또한 매우 우수하다는 것을 나타낸다.As a result of analyzing each extract by HPLC under equivalent conditions, as shown in FIG. 2 , the peak of Example 1e contained both the peaks of Comparative Example 1e and Comparative Example 2e, and it was found that the content was high. This indicates that, according to the extraction method of the present invention, both water-soluble and oil-soluble effective ingredients can be extracted, and the efficiency is also very excellent.
실시예 4. 화장료 조성물의 제조Example 4. Preparation of a cosmetic composition
실시예 4-1. 유연 화장수(스킨) 조성Example 4-1. Composition of flexible lotion (skin)
실시예 1a의 추출물을 사용한 유연 화장수(스킨) 조성의 예시는 다음과 같다.An example of the composition of a softening lotion (skin) using the extract of Example 1a is as follows.
실시예 4-2. 영양화장수(로숀) 조성Example 4-2. Nutrient lotion (lotion) composition
실시예 1a의 추출물을 사용한 영양화장수(로숀) 조성의 예시는 다음과 같다.An example of the composition of a nutrient lotion (lotion) using the extract of Example 1a is as follows.
실시예 4-3. 영양크림 조성Example 4-3. Nourishing cream composition
실시예 1a의 추출물을 사용한 영양크림 조성의 예시는 다음과 같다.An example of the composition of the nourishing cream using the extract of Example 1a is as follows.
실시예 4-4. 팩 조성Example 4-4. pack composition
실시예 1a의 추출물을 사용한 팩 조성의 예시는 다음과 같다.An example of the pack composition using the extract of Example 1a is as follows.
실시예 4-5. 맛사지 크림 조성Example 4-5. Massage cream composition
실시예 1a의 추출물을 사용한 맛사지 크림 조성의 예시는 다음과 같다.Examples of the composition of the massage cream using the extract of Example 1a are as follows.
실험 결과, 실시예 1의 추출물이 종래 통상적인 화장료 성분과 잘 융화되어, 에센스, 유연화장수, 영양유액, 영양크림 등의 화장료 제형이 잘 제조되는 것을 확인하였다.As a result of the experiment, it was confirmed that the extract of Example 1 was well compatible with conventional cosmetic ingredients, and cosmetic formulations such as essence, softening lotion, nutrient emulsion, and nutrient cream were well prepared.
Claims (9)
상기 단계는
상기 인지질, 계면활성제 및 인지질 이외의 지질을 혼합하여 유상부를 제조하는 단계;
상기 수용성 용매 및 물을 혼합하여 수상부를 제조하는 단계 및
상기 추출 원료에 상기 유상부 및 수상부를 가하고 교반하는 단계를 포함하는, 추출 방법.The method of claim 1,
The step is
preparing an oil phase by mixing lipids other than the phospholipids, surfactants and phospholipids;
preparing an aqueous phase by mixing the water-soluble solvent and water; and
An extraction method comprising adding and stirring the oil phase part and the aqueous phase part to the extraction raw material.
상기 계면활성제는 스팬(span)형 계면활성제, 트윈(tween)형 계면활성제 및 비이온성 계면활성제로 이루어진 군으로부터 선택되는 하나 이상인, 추출 방법.The method of claim 1,
The surfactant is at least one selected from the group consisting of span type surfactants, twin type surfactants and nonionic surfactants, extraction method.
상기 인지질 이외의 지질은 탄소수 12 이상의 지질인, 추출 방법.The method of claim 1,
The extraction method, wherein the lipid other than the phospholipid is a lipid having 12 or more carbon atoms.
상기 인지질 이외의 지질은 지방산 에스테르 및 식물성 오일로 이루어진 군으로부터 선택되는 하나 이상인, 추출 방법.5. The method of claim 4,
The extraction method, wherein the lipids other than the phospholipids are at least one selected from the group consisting of fatty acid esters and vegetable oils.
상기 수용성 용매는 탄소수 2 내지 6의 알코올인, 추출 방법.The method of claim 1,
The water-soluble solvent is an alcohol having 2 to 6 carbon atoms, extraction method.
A food composition comprising the extract of claim 7.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20030084120A (en) * | 2002-04-25 | 2003-11-01 | 한불화장품주식회사 | Nanovesicle and cosmetics including same |
| KR100963093B1 (en) | 2008-01-15 | 2010-06-14 | (주)아모레퍼시픽 | Method for extracting using Himalayan Glacial Water and the extracts thereof, and cosmetic composition containing nanoparticles encapsulated the extracts |
| KR101526266B1 (en) | 2008-02-04 | 2015-06-05 | (주)아모레퍼시픽 | Extraction method using deep sea water and the extracts thereby, and the cosmetic composition containing nanoparticles encapsulated the extracts |
| KR20170099423A (en) * | 2016-02-23 | 2017-09-01 | 신성대학 산학협력단 | Extraction method of vegetable effective components using vesicles |
| JP2017171649A (en) * | 2016-03-18 | 2017-09-28 | 日光ケミカルズ株式会社 | Having liposome compositions with high percutaneous absorption properties, and cosmetics or skin external preparations containing the same |
| KR20190041738A (en) * | 2017-10-13 | 2019-04-23 | 김동명 | Vehicle for cosmetic composition having loading capacity of high-content pooly-soluble material and composition for cosmetics comprising the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20030084120A (en) * | 2002-04-25 | 2003-11-01 | 한불화장품주식회사 | Nanovesicle and cosmetics including same |
| KR100963093B1 (en) | 2008-01-15 | 2010-06-14 | (주)아모레퍼시픽 | Method for extracting using Himalayan Glacial Water and the extracts thereof, and cosmetic composition containing nanoparticles encapsulated the extracts |
| KR101526266B1 (en) | 2008-02-04 | 2015-06-05 | (주)아모레퍼시픽 | Extraction method using deep sea water and the extracts thereby, and the cosmetic composition containing nanoparticles encapsulated the extracts |
| KR20170099423A (en) * | 2016-02-23 | 2017-09-01 | 신성대학 산학협력단 | Extraction method of vegetable effective components using vesicles |
| JP2017171649A (en) * | 2016-03-18 | 2017-09-28 | 日光ケミカルズ株式会社 | Having liposome compositions with high percutaneous absorption properties, and cosmetics or skin external preparations containing the same |
| KR20190041738A (en) * | 2017-10-13 | 2019-04-23 | 김동명 | Vehicle for cosmetic composition having loading capacity of high-content pooly-soluble material and composition for cosmetics comprising the same |
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