KR100716066B1 - Diet Food Comprising Eucommia-ulmoides Oliver Extract and Morus-alba Linne Extract - Google Patents

Abstract

The present invention relates to a method of inhibiting differentiation into adipocytes by treating the cedar leaf extract or the upper leaf extract on stem cells, and a food having an anti-obesity effect, including the former cedar leaf extract or the upper leaf extract. Foods exhibiting the anti-obesity effect of the present invention can be widely used as a food for suppressing obesity because it comprises a composition consisting of the extract of the cedar leaf and the upper lobe, which is most effective in suppressing the differentiation into adipocytes, but also excellent in palatability .

Cedar leaf extract, leaf extract, anti-obesity

Description

Diet Food Comprising Eucommia-ulmoides Oliver Extract and Morus-alba Linne Extract             

Figure 1a is a fluorescence micrograph showing the adipocytes differentiated by one differentiation induction process.

Figure 1b is a fluorescence micrograph showing the adipocytes differentiated by two differentiation induction process.

Figure 1c is a fluorescence micrograph showing the adipocytes differentiated by three differentiation induction process.

Figure 2a is a graph showing the quantitative change of fat contained in the differentiated adipocytes, according to the concentration change of the extract of the upper leaf.

Figure 2b is a graph showing the quantitative change of fat contained in the differentiated adipocytes, according to the concentration change of the cedar leaf extract.

Figure 2c is a graph showing the quantitative change of fat contained in the differentiated adipocytes, according to the concentration changes of the extract, green tea extract and tangerine peel extract.

Figure 3a is a photograph showing the two-dimensional electrophoresis results of cells not treated with the upper lobe and the cedar leaf extract.

Figure 3b is a photograph showing the results of two-dimensional electrophoresis of the cells treated with the upper leaf and the cedar leaf extract.

The present invention relates to a method of inhibiting differentiation into adipocytes by treating the cedar leaf extract or the upper leaf extract on stem cells, and a food having an anti-obesity effect, including the former cedar leaf extract or the upper leaf extract.

Obesity is a phenomenon in which most of the calories ingested are consumed and the remaining parts are converted into adipocytes and accumulate in various parts of the body, especially the subcutaneous tissue and the abdominal cavity. Since the size of the fat cells is limited, in order to store energy quickly when excess energy is ingested, an increase in the number of fat cells is required, thereby creating new fat cells in the human body. New adipocytes are differentiated from pro-adipocytes. Progenitor cells are formed from differentiation from stem cells. Hormones and growth factors are involved in the process of stem cells from adipocytes through adipocytes. And bioregulators such as cytokines are affected.

Recently, a lot of efforts have been made to prevent and treat obesity, and diet and exercise therapy such as calorie / energy and basic metabolic rate control have become popular. However, the root cause of obesity is an increase in the number of mast cells, and if the failure to suppress the excessive production of mast cells in the human body, obesity can recur at any time, so the popular therapy cannot fundamentally cure obesity . In particular, in the case of childhood obesity caused by an abnormally excessive increase in the number of mast cells in the human body, it is known that the possibility of treatment is very low, unless the increase of mast cells is suppressed.

Therefore, through various studies, it was intended to develop a method for preventing and treating obesity. For example, Korean Patent No. 52299 discloses an anti-obesity agent using dehydroepiandrosterone (DHA) as an active ingredient, and Korean Patent No. 181178 discloses a substituted 1,3-benzodioxol compound as an active ingredient. An anti-obesity agent is disclosed, and Korean Patent No. 149679 discloses an anti-obesity agent using an aromatic amino-alcohol derivative as an active ingredient, and Korean Patent No. 127504 discloses an anti-obesity yogurt, and Korean Patent No. 348818 An anti-obesity green tea and a manufacturing method thereof are disclosed, and Korean Patent Application No. 2003-63853 discloses a manufacturing method of an obesity prevention sandwich using eggplant extract as an active ingredient, and Korean Patent Application No. 2003-9941 discloses An anti-obesity composition comprising natural dietary fibers is disclosed.

However, various technologies developed so far include a substance that inhibits the absorption and biosynthesis of fats as an active ingredient, so that excessive fat accumulation in fat cells such as adult obesity can be partially suppressed, but fat cells increase. It is pointed out that there is no effect on the phenomenon, and efforts are being made to develop a substance that can suppress the increase of fat cells, but there are no results.

Therefore, there is a constant need to develop a method that can suppress the increase of fat cells in the body inherently.

Accordingly, the present inventors have made intensive studies to develop a method for inhibiting the increase of fat cells in the body, when stem cells are treated with the cedar leaf extract, the upper leaf extract or a mixture thereof, stem cells differentiate into adipocytes. By suppressing the process, as a result, it is possible to suppress the increase in the number of adipocytes, it was confirmed that the cedar leaf extract or the upper leaf extract can be ingested in the form of food, and completed the present invention.

As a result, the main object of the present invention is to provide a method of treating the cedar leaf or the upper leaf extract to inhibit differentiation into adipocytes.

It is another object of the present invention to provide an obesity inhibiting food comprising the above-mentioned cedar leaf and upper leaf extract.

The method of inhibiting differentiation into adipocytes of the present invention includes treating the stem cells with the upper leaf extract, the cedar leaf extract, or a mixture thereof: wherein the method for obtaining the upper leaf extract and the green leaf extract is particularly limited thereto. However, it is preferable that the upper lobe or the two larvae are obtained by hot water extraction or ethanol extraction.

The upper leaves are the leaves of the mulberry (Morus alba Linne.) Belonging to the genus Morracerae, and are broad oval or egg-shaped. The upper lobe is the hormones inocosterone, ecsterone, and triterpenoids β-sitosterol-β-glucoside, β-sitosterol, flavonoids rutin, Moracetin, isoquercitrin, coumarins umbeliferon and scopol It contains more than 20 kinds of substances including retin and the like, and is known to be effective in the treatment of anti-galvanic effect, hypoglycemic effect, blood pressure lowering effect, antibacterial action, anti-inflammatory effect, diabetes.

In describing the present invention, "upper leaf extract" means an extract obtained by hot water extraction or ethanol extraction of the upper leaf, specifically, the upper leaf is immersed in water so as to be 20 to 30% by weight, and 1 to 80 to 100 ° C. After heating for 5 hours, the hot water extract or upper leaf, which is a liquid component obtained by filtration, is immersed in ethanol to 20 to 30% by weight, left at a temperature of 40 to 60 ° C for 1 to 5 hours, and then obtained by filtration. It means an ethanol extract which is a liquid component, and in some cases, it may mean a dry powder obtained by vacuum drying each extract.

It is a deciduous tree (Eucommia ulmoides Oliver) belonging to the Eucommiaceae, which is oval or long oval and serrated. Both leaf lobes include chlorogenic acid, caffeic acid, acuvin, gum, glycoside, rogannine, flavonoid, alkaloid, organic acid, resin, vitamin C, etc., and anti-aging, anti-fatigue, glucosidase inhibition (glucosidase) It is known to have the effects of inhibition (inhibition), cardiac contractility, vasodilation, and anti-diabetic effect.

In describing the present invention, the term "cedar leaf extract" refers to an extract obtained by extracting hot water or ethanol extract of the mandarin leaf, specifically, the soybean leaf is immersed in water to be 20 to 30% by weight, and 80 to 100 ° C. After heating for 1 to 5 hours, immersed in ethanol to 20 to 30% by weight of the hot water extract or the upper leaf of the liquid component obtained by filtration, and left for 1 to 5 hours at a temperature of 40 to 60 ℃, It means an ethanol extract which is a liquid component obtained by filtration, and in some cases, it may mean a dry powder obtained by vacuum drying each extract.

The present inventors searched for substances that can safely and effectively suppress the increase of fat cells, which are the main cause of obesity, mainly on natural products. In other words, after culturing stem cells isolated from human bone marrow, inducing differentiation into adipocytes, the morphology of the cells changes to a round shape, the number of lipid vacuoles increases, and the synthesis of triglycerides. As the amount increases, it turns into fat cells. Thus, natural products extract was added to the differentiation induction process, and by analyzing the effects on the differentiation of adipocytes, the natural products to effectively inhibit the differentiation process of adipocytes were searched. As a result, it was confirmed that the cedar leaf extract and the upper leaf extract can effectively inhibit the differentiation of adipocytes. From this, it was found that the cedar leaf and the upper leaf extracts can exhibit an obesity inhibitory effect.

In addition, the present inventors studied a method that can exhibit the most effective anti-obesity effect using the electric two-leaf extract and the upper leaf extract, as a result of ingesting the electrical extract as it is or by adding the electrical extract to food, After adding the extract to the food to prepare a form of food that exhibits an anti-obesity effect, it was found that ingesting it is more effective.

Thus, the food exhibiting the anti-obesity effect of the present invention includes a composition of the two leaf extracts, the upper leaf extracts or the two leaf extracts and the upper leaf extracts composed of 3: 7 to 6: 4 (w / w): Is not particularly limited thereto, but may be prepared by addition in preparation of various foods including wire, noodles, confectionary, beverages, meat, fish, herbs, steamed rice or rice, and the content of the electrical composition included in the food Although not particularly limited thereto, when the powder is ingested in the form of a wire, the composition is preferably contained in an amount of 1 to 50% by weight based on the final weight of the food. In the case of ingestion in addition to foods such as meat, fish, herbs, steamed rice or rice, the composition comprises 1 to 10% by weight relative to the final weight of the food. It is preferred.

In addition, in order to most effectively suppress the differentiation into adipocytes, but to add to foods, to determine the mixing ratio of the cedar leaf extract and the upper leaf extract contained in the composition, which was excellent in palatability, as a result of various studies, the cedar leaf extract and the upper leaf extract When the composition consisting of 3: 7 to 6: 4 (w / w) was used, it was found that the most effective suppression of differentiation into adipocytes, but excellent taste when added to food.

Foods exhibiting the anti-obesity effect of the present invention can be widely used as a food for suppressing obesity because it comprises a composition consisting of the extract of the cedar leaf and the upper lobe, which is most effective in suppressing the differentiation into adipocytes, but also excellent in palatability .

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Example 1 Effects of Cerebral Foliar and Upper Leaf Extracts on Adipocyte Differentiation

In the process of differentiating human mesenchymal stem cells into adipocytes, cedar leaf extract, upper leaf extract, green tea extract, Macmundong extract, and tangerine peel extract were treated, respectively, and the degree of differentiation of adipocytes was measured.

Example 1-1 : Obtaining Extracts

Tobacco leaf, upper leaf, green tea, green tea, mackmundong and tangerine peel 300g immersed in 1L of water, and extracted by heating to 100 ℃ for 4 hours, and then filtered to take the liquid components, concentrated and dried to obtain a powder obtained as an extract .

Example 1-2 adipocyte differentiation

Human mesenchymal stem cells (hMSCs) isolated from human bone marrow were inoculated in DMEM (Sigma Chem. Co., USA) containing 10% (w / v) FBS, penicillin and streptomycin, pH Incubated for 3 days in a CO ₂ incubator at 7.2, 37 ° C., 5% (v / v) and passaged under the same conditions.

Subcultured hMSCs were transferred to 12 well plates, inoculated to 10 ⁴ cells per well, incubated for 7 days until confluent, and then differentiated induction medium (10% (w / v) FBS, DMEM, 10 μg). / Ml insulin, 1μM dexamethasone, 0.5mM methyl-isobutylxanthine, 100μM indomethacin for 72 hours, adipocyte maintenance (AM) medium (10% (w / v) FBS, DMEM, 10μg / ml insulin) , Differentiation induction process of 10 μg / ml d-biotin) for 24 hours was repeated one, two and three times to differentiate hMSCs into adipocytes.

When differentiated into adipocytes, lipid vacuole accumulates in the cytoplasm, and when the electric cells are dyed using Oil red O, a dye that can stain only fatty acids and cholesterol, the degree of staining is different from that of adipocytes. Since it is proportional to and treated with the oil red O dye to the differentiated adipocytes, the degree of differentiation was indirectly measured by fluorescence microscopy (see FIGS. 1A, 1B and 1C). Figure 1a is a fluorescence microscope picture showing the adipocytes differentiated by one differentiation induction process, Figure 1b is a fluorescence microscope picture showing the adipocytes differentiated by two differentiation induction process, Figure 1c is a differentiation induction process Fluorescence micrograph showing the adipocytes differentiated by three runs. As shown in Figure 1a to 1c, it can be seen that as the differentiation induction process is repeated hMSC is significantly differentiated into adipocytes.

Example 1-3 : Effect of Adipocyte Differentiation on Each Extract

Each extract obtained in Example 1-1 was added to the differentiation induction medium at concentrations of 0, 0.005, 0.01, 0.05, 0.1, 0.5, 1.0, 5.0, 10, 50 and 100 mg / ml, and each extract was included. After obtaining the differentiated differentiation medium, the differentiation was induced three times using the method of Example 1-2, and then differentiated into adipocytes, and cultured for 7 days. Differentiated fat cells were stained with an Oil red O dye, pulverized, and then absorbed at 500 nm to measure the amount of fat contained in each differentiated cell, thereby measuring the degree of differentiation of each cell (see 2A, 2B and 2C). Figure 2a is a graph showing the quantitative change in fat contained in the differentiated adipocytes, according to the change in the concentration of the upper leaf extract, Figure 2b is a quantitative change in the fat contained in the differentiated adipocytes, according to the change in concentration of the extracts Figure 2c is a graph showing the quantitative change of fat contained in the differentiated adipocytes, according to the concentration change of the makmundong extract (◆), green tea extract (▲) and tangerine peel extract (■). As shown in Figures 2a to 2c, green tea, gingham dong and tangerine peel extract did not inhibit the adipocyte differentiation, it was found that the extracts of the upper lobe and cedar leaf effectively inhibited the adipocyte differentiation.

For reference, in the case of treatment at a concentration of 100 mg / ㎖ in Figure 2c, the amount of fat was sharply reduced, but because it is an effect of apoptosis, it is not related to inhibition of differentiation.

Example 1-4 : Influence of Upper and Two-Legal Extracts on Adipocyte Differentiation

In Example 1-3, it was shown that the upper lobe and the two mesenchyme can inhibit the differentiation into adipocytes, which was specifically confirmed.

That is, differentiation-inducing medium containing no extract (control group), differentiation-inducing medium containing the upper leaf extract at a concentration of 1 mg / ml (experimental group 1), and differentiation-inducing medium containing the cedar leaf extract at a concentration of 1 mg / ml. (Experimental group 2) and differentiation-inducing medium (Experimental group 3) containing 1 mg / ml of upper leaf extract and 1 mg / ml of cedar leaf extract were prepared, and three differentiation induction was carried out using the method of Example 1-2 above. The cells were differentiated into adipocytes by culturing and cultured for 7 days. Then, the amount of fat contained in each of the differentiated cells was measured by the method of Examples 1-3 (see Table 1).

The amount of fat contained in each differentiated cell Experimental group Amount of fat (μg) ratio(%) Control 138.45 ± 0.12 100 Experimental group 1 35.22 ± 0.07 25.4 Experiment group 2 38.14 ± 0.05 27.5 Experiment group 3 12.45 ± 0.04 9.0

As shown in Table 1, the upper leaf extract and the cedar leaf extract significantly reduced the amount of fat, it can be seen that the upper leaf extract and the cedar leaf extract can effectively inhibit the differentiation into adipocytes. In particular, it was found that differentiation into adipocytes was hardly performed when the upper lobe and the two lobe were mixed.

Therefore, it was confirmed that the upper leaf extract and the cedar leaf extract showed the effect of inhibiting obesity.

Example 2 Determination of the Mixing Ratio of the Upper Leaf Extract and the Two Leaf Extract

As shown in Examples 1-4, the upper leaf extract and the cedar leaf extract exhibited the effect of inhibiting the differentiation into adipocytes, and when the upper leaf extract and the cedar leaf extract were used in combination, it was found that it effectively inhibited the differentiation into adipocytes. As a result, we tried to determine the mixing ratio of the upper leaf extract and the cedar leaf extract that can most effectively inhibit the differentiation into adipocytes.

That is, the two leaf extract and the upper leaf extract obtained in Example 1-1 were 1: 9, 2: 8, 3: 7, 4: 6, 5: 5, 6: 4, 7: 3, 8: 2 and Each composition was mixed at a ratio of 9: 1 (w / w), and was added to the differentiation-inducing medium at a concentration of 2 mg / ml to obtain the differentiation-inducing medium containing the respective extracts. Using the method of -2, three differentiation induction was performed to differentiate into adipocytes and cultured for 7 days. Differentiated fat cells were stained with an Oil red O dye, pulverized, and then absorbed at 500 nm to measure the amount of fat contained in each differentiated cell, thereby measuring the degree of differentiation of each cell (see Table 2). At this time, as a control group was used adipocytes differentiated into differentiation-inducing medium to which no cedar leaf extract and the upper leaf extract was added.

Quantitative Changes of Fat in Adipocytes According to Composition Ratio of Extracts Mixed ratio (head lobe: upper leaf, w / w) Amount of fat (μg) ratio(%) Control 123.45 ± 0.12 100 1: 9 36.49 ± 0.07 26.4 2: 8 34.71 ± 0.12 25.0 3: 7 15.42 ± 0.11 11.1 4: 6 14.16 ± 0.09 10.2 5: 5 12.45 ± 0.04 9.0 6: 4 11.24 ± 0.06 8.1 7: 3 11.06 ± 0.08 7.9 8: 2 10.97 ± 0.07 7.9 9: 1 10.88 ± 0.01 7.9

As shown in Table 2, when the mixing ratio of the cedar leaf extract and the upper leaf extract is 3: 7, 4: 6, 5: 5 and 6: 4 (w / w), the amount of fat contained in the differentiated adipocytes Significantly decreased, it was found that the amount of fat remained at a certain level at 7: 3, 8: 2 and 9: 1 (w / w). From this, when the mixture of the cedar leaf extract and the upper leaf extract with the mixing ratio of 3: 7, 4: 6, 5: 5 and 6: 4 (w / w) significantly reduced the number of adipocytes, the mixing ratio was significantly reduced. When admixture of the mixture of the two leaf extracts and the upper leaf extracts of 7: 3, 8: 2 and 9: 1 (w / w), it could be predicted that the adipocytes remained constant in number.

Example 3 Effect of Adipose Leaf Extract on Adipocyte Differentiation

Two-dimensional electrophoresis was performed on the control group and Experiment group 2 of Examples 1-4, and the difference was analyzed by MALDI-TOF analysis.

Example 3-1 two-dimensional electrophoresis

Cell lysis buffer solution (8M urea, 4% CHAPS (3-[(3-cholamidopropyl) dimethylammonio-1-propanesulphonate), 0.0016 g / Ml protein inhibitor coktail) was used to obtain cell extracts, which were then applied to an immobilized pH gradient strip (pH gradient dry strip, Amersham Pharmacia Biotech, Sweden) with a length of 13 cm, pH 3.0 to 10.0, at 200 V for 1 hour, Isoelectric focusing (IEF) was performed under electrical conditions of 1 hour at 500 V, 1 hour at 2000 V, and 8000 to 96,000 Vhr.

Subsequently, the solution was equilibrated by immersion in TBP equilibration buffer (6M urea, 2% SDS, Tris / HCl gel buffer, 20% glycerol, 5 mM TBP, 2.5% acrylamide, pH 8.8), and then a concentration gradient SDS of 10% to 12.5%. Electrophoresis gel (5X Tris (pH 8.8), 40% acrylamide, 50% glycerol, 10% SDS, 100 mM sodium thiosulfate, 16% ammonium persulfate, TEMED), followed by 10 mA electrophoresis for 25 minutes, followed by 30 mA After electrophoresis with electric current, nitric acid was stained by staining method, it was compared and analyzed using a 2-D analysis program (Elite software, Amersham Pharmacia Biotech. Co., Sweden) (see FIGS. 3A and 3B). Figure 3a is a photograph showing the two-dimensional electrophoresis results of cells not treated with the upper lobe and the cedar leaf extract, Figure 3b is a photograph showing the two-dimensional electrophoresis results of cells treated with the extract of the upper lobe and the two leaves. As shown in Figure 3a and 3b, it can be seen that there is a difference between the control group and the experimental group 2 for many proteins.

Example 3-2 Analysis of Proteins Using the MALDI-TOF Assay

As shown in the results of the previous Example 2-1, there are differences between the control group and the experimental group 2 for many proteins, but to specifically identify the effect of the cedar leaf extract with only the results of the newly appearing protein, or decrease / increase it's difficult. That is, since the role of the two lobes or the effects of the two lobes are not known unless the properties of each protein are known, the proteins significantly increased after the administration of the two lobe extracts were analyzed using the MALDI-TOF assay.

First, the desired protein of Experimental Group 2 not found in the control group was separated from the electrophoretic gel of Example 2-1, decolorized, treated with 70% (v / v) acetonitrile for 5 minutes, and 100% aceto. The gel was dehydrated and dried by treatment with nitrile for 5 minutes. Subsequently, the dried gel was immersed in proteolysis buffer (25 mM ammonium carbonate, trypsin, pH 7.8), reacted at 4 ° C. for 30 minutes, and then reacted with fresh proteolysis buffer solution at 37 ° C. for 16 hours, followed by centrifugation. The supernatant was separated and then lyophilized to obtain a sample for MALDI-TOF analysis.

Add 8 μl of Buffer A (50% acetonitrile, 0.5% trifluoroacetic acid) to the sample, mix 8 μl of matrix solution (50% acetonitrile, 0.5% trifluoroacetic acid, α-cyano-4-hydroxy cinnamic acid) 0.4 μl was injected into the MALDI-TOF analyzer (Ettan MALDI-TOF pro system, Amersham Pharmacia Biotech, Sweden) and analyzed. At this time, the mass range was set to 800 to 3000.

As a result, tropomyosin (tropomyosin) was measured as a protein that does not appear in the control group, but appears only in Experimental Group 2. Since this protein is mainly expressed in differentiated muscle cells, It was confirmed once again that differentiation was not performed normally.

Example 4 Preparation of Foods Having an Obesity Inhibitory Effect

A composition consisting of the upper leaf extract and the two leaf extracts was added to prepare a normal food, and a food having an anti-obesity effect was prepared as follows.

Example 4-1 Preparation of Diet Noodles

Dough was prepared by mixing 10 g of lettuce extract, 10 g of cedar leaf extract, 700 mg of wheat flour, 80 g of eggs, and 200 g of water, and a noodles were prepared by a conventional noodle manufacturing method, thereby preparing a diet noodle having an anti-obesity effect.

Example 4-2 Preparation of Diet Biscuits

A dough was prepared by mixing 10 g of lettuce extract, 10 g of cedar leaf extract, 700 mg of wheat flour, 200 g of milk, and 80 g of eggs, and a diet biscuit having an anti-obesity effect was prepared by a conventional biscuit manufacturing method.

Example 4-3 Preparation of Diet Drinks

10 g of lettuce extract, 10 g of cedar leaf extract, 10 mg of fruit flavor, 100 g of fruit juice, 10 mg of sodium benzoate and 900 ml of water were mixed to prepare a diet beverage having an anti-obesity effect.

Example 4-4 Preparation of Meat Diet Spices

10 g of lettuce extract, 10 g of cedar leaf extract, 100 g of pear juice, 100 g of radish juice, 300 g of red pepper paste, a small amount of salt, garlic, pepper, onion and ginger were mixed to prepare a meat seasoning.

Example 4-5 Preparation of Diet Bean Sprouts

10 g of lettuce extract, 10 g of cedar leaf extract, 150 g of red pepper powder, a small amount of salt, garlic, pepper, onion, and ginger are mixed to prepare a seasoning for bean sprouts, 200 g of the previously prepared seasoning and 500 g of bean sprouts are mixed. By the method, diet sprouts toppings were prepared.

Example 4-6 Preparation of Diet Miso Stew

10 g of lettuce extract, 10 g of cedar leaf extract, 600 g of soybean paste, a small amount of salt, garlic, pepper, onion, and ginger were mixed, put into 500 ml of water, and cooked by a conventional method to prepare a diet miso crab.

Example 4-6 Preparation of Diet Grain Rice

10 g of upper leaf extract, 10 g of cedar leaf extract, 50 g of black soybean, 50 g of sorghum, 50 g of millet and 300 g of white rice were mixed and cooked in a conventional manner to prepare a diet grain rice.

Example 5 sensory evaluation

Each diet drink was prepared by varying the mixing ratio of the upper leaf extract and the two leaf extracts contained in the diet drink prepared in Example 4-3, and then the sensory test was performed.

In other words, the two leaf extracts and the upper leaf extracts of 1: 9, 2: 8, 3: 7, 4: 6, 5: 5, 6: 4, 7: 3, 8: 2 and 9: 1 (w / w) 20 g of each composition mixed in the ratio was obtained, and each diet drink was mixed with 10 mg of fruit flavor, 100 g of fruit juice, 10 mg of sodium benzoate, and 900 ml of water to prepare each diet beverage having an anti-obesity effect.

Then, each of the electric beverages prepared by each of them was tasted by 15 inspectors, and the taste, flavor, color, and general taste were divided into 5 grades according to good or bad, and very good 5 points, good 4 points, and normal 3 points. It was bad, 2 points | pieces, and very bad. It evaluated by 1 point | piece and averaged it (refer Table 3).

Sensory test result of diet drink Mixed ratio (head lobe: upper leaf, w / w) flavor Flavor Color General Symbol 1: 9 3.4 3.7 3.4 3.6 2: 8 3.6 3.6 3.5 3.6 3: 7 3.7 3.9 3.7 3.7 4: 6 3.9 3.8 3.8 3.8 5: 5 4.1 3.7 3.9 4.1 6: 4 4.0 3.9 3.8 4.0 7: 3 3.8 3.8 3.6 3.8 8: 2 3.2 3.9 3.3 3.5 9: 1 2.9 3.6 3.2 3.1

As shown in Table 3, the diet drink containing the composition of the two leaf extract and the upper leaf extract in a ratio of 3: 7, 4: 6, 5: 5, 6: 4 and 7: 3 (w / w) Although relatively high palatability, diet beverages comprising a composition in which the cedar leaf extract and the upper leaf extract were mixed at a ratio of 1: 9, 2: 8, 8: 2, and 9: 1 (w / w) had a relatively low palatability. It could be seen that.

Combining the results of Example 2 and Example 5, when using the composition consisting of the two leaf extract and the upper leaf extract 3: 7 to 6: 4 (w / w), not only effectively inhibits the differentiation into adipocytes When added to the food it was found that excellent palatability.

As described and demonstrated in detail above, the present invention provides a method for inhibiting the differentiation into adipocytes by treating the cedar leaf extract or the upper leaf extract to stem cells and foods exhibiting the anti-obesity effect, including the former cedar leaf extract or upper leaf extract do. Foods exhibiting the anti-obesity effect of the present invention can be widely used as a food for suppressing obesity because it comprises a composition consisting of the extract of the cedar leaf and the upper lobe, which is most effective in suppressing the differentiation into adipocytes, but also excellent in palatability .

Claims (5)

delete delete delete Two leaf lobe extracts obtained by hydrothermal extraction or ethanol extraction of the cedar leaf and the upper leaf extract obtained by hot water extraction or ethanol extraction of the upper leaf comprises a composition consisting of 3: 7 to 6: 4 (w / w), Food that has anti-obesity effect by preventing differentiation into fat cells. The method of claim 4, wherein Characterized in the form of wire, noodles, sweets, beverages, meat, fish, herbs, steamed rice or rice Foods that show the effects of obesity.

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