KR20180062930A - Food composition comprising dendropanax morbifera lev. extract for preventing or improving tiredness, muscle weakness and reduced exercise capacity due to andropause - Google Patents

Abstract

The present invention relates to a food composition comprising a Dendropanax morbifera extract for reducing symptoms due to andropause selected from tiredness, muscle weakness and reduced exercise capacity, and more specifically, to a food composition for preventing and alleviating andropause diseases and andropathy, which comprises a hot water extract of the Dendropanax morbifera or fractions from the hot water extract of the Dendropanax morbifera as an active ingredient to be able to reduce symptoms caused by the andropause including tiredness, muscle weakness and the like.

Description

TECHNICAL FIELD [0001] The present invention relates to a functional food composition containing an extract of Euphorbiaceae extract which improves fatigue, muscle weakness and lowering of exercise performance due to menopausal period. [0002] FOOD COMPOSITION COMPRISING DENDROPANAX MORBIFERA LEV. EXTRACT FOR PREVENTING OR IMPROVING TIREDNESS, MUSCLE WEAKNESS AND REDUCED EXERCISE CAPACITY DUE TO ANDROPAUSE}

The present invention relates to a composition for preventing and ameliorating male menopausal symptoms, which comprises hot-water extract of Hwangchu-wood. More particularly, the present invention relates to a composition for preventing and ameliorating male menopausal disorders, Lt; / RTI >

Women become menopausal and female hormones are rapidly decreased, resulting in several visible changes, which are called menopause. The term "normal menopausal period" has been used only in women, but in recent years, the concept of male menopausal syndrome is emerging as a result of the gradual decrease of the male hormone in the body due to aging.

Male hormones are steroid hormones, testosterone is a representative male hormone. Testosterone is mainly produced in Leydig cells of the testis and exists in a free form or in a form associated with blood proteins, albumin and sex hormone binding globulin (SHBG) . When testosterone binds to SHBG, it loses its activity, and when separated, it acts as a hormone. The testes are regulated by the follicle stimulating hormone (FSH) and the luteinizing hormone (LH) secreted from the pituitary gland. FSH and LH stimulate the gonads to differentiate and mature To promote spermatogenesis. Lydia cells have LH receptors, and LH is known to promote the synthesis and secretion of testosterone in Lydia cells.

The term "menopausal menopause" refers to the term "late-onset hypogonadism (LOH)" as determined by the International Society for the Study of the Aging Male in 2004, Age-associated testosterone deciency syndrome (TDS). Andropause is a clinical and biochemical syndrome associated with increased age, defined as a typical symptom and a deficiency of serum testosterone levels, which can negatively affect the function of various organs. Concurrent symptoms include decreased sexual desire and erectile function, nighttime depression, decreased cognitive ability, decreased fatigue, weight and muscle mass, decreased muscle strength, increased visceral fat, and skin atrophy.

It is argued that the use of male hormone replacement therapy in a manner similar to the use of estrogen replacement therapy in postmenopausal women. According to one study, significant improvement in the effects of treating male menopausal patients with 40 x 2 mg / day of testosterone undecanoate has been reported. However, administration of male hormones has also been associated with the risk of adversely affecting liver, lipid conditions, cardiovascular and prostate diseases. In addition, in terms of adenomas and adenocarcinomas, it is known that about 30% of patients with symptoms attributable to male menopause can not receive male hormone replacement therapy. Therefore, there is a need for the development of new therapeutic agents useful in the prevention and treatment of male menopausal syndromes caused by aging, or chemical or surgical castration, which do not provide disadvantages of hormone replacement therapy.

In order to overcome the problems caused by the conventional hormone replacement therapy in relation to the menopausal disease, it is desired to improve the male menopausal syndrome by using a composition containing the natural herbal medicine as the main ingredient, An attempt is being made. Dendropanax morbifera Lev . ) Is an evergreen broad-leaved arboreous tree native to the southern coast of Korea and Jeju-do. It is a species that does not fall into winter when it blooms in the winter. When it bruises the bark, yellowish resinous liquor is called 黄 漆. Huangchil has been used as a rare paint for the emperor's armor, helmet, and other metal ornaments since the Three Kingdoms period. It has been used in the Goryeo period, which was written in the Goryeo period, the Guilin variety in China, And it is said that it was a special product of Baekje which was before it, and it remains in the passage of the book department of the Tang history book. It is said that it is effective for burning removal, eye quality, burn treatment, leprosy, harmlessness, and it is used recently for tea and food.

KR 1014432300000 B1

It is an object of the present invention to provide a food composition for preventing, ameliorating, or treating male menopausal syndrome, which comprises an extract of Hwangbuk tree as an active ingredient.

According to one aspect of the present invention, there is provided a dendropanax morbifera Lev . ) A functional food composition for improving male menopausal symptoms selected from fatigue, muscular weakness, or impaired exercise capacity containing hot water or hot pepper extract fraction as an active ingredient is provided.

In addition, the Hwangbok tree hydrothermal extract can be prepared by adding 5 to 30 times as much volume of water to woodweed and extracting it at 100 to 130 ° C for 3 to 6 hours.

The fragrant woody fraction can be obtained by fractionating at least one member selected from the group consisting of hexane, chloroform, ethyl acetate and butanol with a fraction solvent.

In addition, the above-described Wuchu-cuticle fraction may be an ethylacetate fraction obtained by successively fractionating the Hwangchu-jatu extract in hot water with hexane, chloroform and ethyl acetate.

In addition, the functional food composition may further include at least one selected from the group consisting of food-acceptable food-aid additives, carriers, excipients and diluents.

In addition, the functional food composition may be prepared in any one form selected from the group consisting of granules, powders, tablets, capsules, liquid solutions and rings.

According to another aspect of the present invention, there is provided a functional food additive for improving menopausal symptoms, which comprises the functional food composition.

According to another aspect of the present invention, there is provided a health functional food for improving male menopausal symptoms, which comprises the functional food composition.

The food composition containing the extract of Huangchuang as an active ingredient according to one aspect of the present invention can promote the secretion of testosterone in the body and enhance the activity. This can be used in the food industry for the purpose of preventing and ameliorating menopausal disorders and male diseases because it can alleviate symptoms caused by menopausal symptoms, including muscle weakness and fatigue.

In addition, the composition comprising the hydrothermal extract of Huangchu tree according to one aspect of the present invention does not induce apoptosis of normal cells, so that it is safe for human body and can be used as a safe food additive.

In addition, the composition comprising the extract of Hwangbuk tree according to one aspect of the present invention can reduce the toxicity and side effects due to the natural plant-derived extract.

Brief Description of the Drawings Fig. 1 is a graph showing the effect of the extract of Huangchu tree hydrothermal extract of the present invention on the blood testosterone secretion in an experimental model of male rats in the old age,
FIG. 2 is a graph showing that the extract of Huangchu tree hydrothermal extract of the present invention has an effect of decreasing SHBG concentration in blood in an old male rat experimental model,
FIG. 3 is a graph showing that the extract of Huangchu tree hydrothermal extract of the present invention has an effect of increasing the secretion of LH and FSH in blood in an old male rat experimental model,
FIG. 4 is a graph showing that DHWH extract of the present invention has an effect of increasing DHEA-s secretion in blood in an experimental male rat model,
FIG. 5 is a graph showing that the extract of Huangchu tree hydrothermal extract of the present invention shows an effect of improving fatigue, muscle strength and athletic performance in an experimental male rat model,
FIG. 6 is a graph showing that the hydrothermal extract of P. japonicus does not show cytotoxicity in TM3 cells,
FIG. 7 is a graph showing the effect of the hydrothermal extract of Hwangcholga extract of the present invention on the increase of testosterone secretion in TM3 cells,
FIG. 8 is a graph showing the effect of the hydrothermal extract of P. japonicus on the increase of testosterone secretion under oxidative stress environment induced by hydrogen peroxide in TM3 cells,
FIG. 9 is a graph showing the effect of the present invention fraction of quercitin on the increase of testosterone secretion under an oxidative stress environment induced by hydrogen peroxide in TM3 cells. FIG.

The invention hwangchil tree (Dendropanax morbifera Lev . ) Functional food composition for prevention or amelioration of male menopausal syndrome which comprises, as an active ingredient, fragrant wood fractions fractionated from a hot-water extract or a hot-water extract of Hwangchu-wood, a health-functional food and a food additive comprising the same.

Hereinafter, the present invention will be described in more detail.

The composition of the present invention contains as an active ingredient an extract of Hwangchujanggae or a fraction of Hwangchujangchae which is fractionated from a hydrothermal extract of Hwangchuok tree.

In order to prepare the hot-water extract or the fraction of the Hwigae-gil, the Hwigae can be prepared by drying.

In the present invention, the portion of the Hwigae-jinja that can separate the Hwangchu-kogyo hot-water extract is not particularly limited, and at least one selected from the group consisting of leaves, branches, roots and fruits of Huaculi can be used. Preferably, at least one selected from the group consisting of leaves, branches and roots of Hwigae-gil. This can be cut, dried and pulverized by a conventional method. It is possible to improve the functionality of Uchihaku by drying it. The drying method is not particularly limited, and for example, natural drying or freeze-drying may be used. If necessary, the wood can be cut or crushed before or after drying.

In the present invention, the above-mentioned hot-water extract of Huangchuang can be obtained by treating the Huangchu-wood raw material with an extraction solvent, subjecting it to hot water extraction, and then concentrating under reduced pressure. In the present invention, water is preferably selected as the extraction solvent. When extracting the extractant by treating the extracting solvent, the extracting solvent may be added at 5 to 30 times the dry weight of the whole wood. If the extraction solvent is less than 5 times or more than 30 times the dry weight of the whole wood, the extraction efficiency may be lowered and the manufacturing cost may increase. Preferably, the extraction solvent is added in an amount of 10 to 20 times the dry weight of the whole wood.

The temperature at which the extract is separated by treating the extraction solvent can be adjusted according to the extraction method. For example, from 80 to 140 캜. The extract may be extracted at the above temperature for 2 to 10 hours to isolate the extract of Fusarium oxysporum; Preferably, the extract is extracted with an extracting machine at 100 to 130 ° C for 3 to 6 hours to isolate the extract of Hwangcholgak tree. More preferably, the extract is extracted at 100 to 120 ° C for 4 to 5 hours to isolate the extract of Hwangjak tree. The separated extract may be provided in the form of a liquid or solid powder having a controlled concentration by removing the solvent through filtration, concentration or drying. Preferably, in order to increase the concentration of the useful component and improve the shelf life, it is preferable to concentrate and freeze-dry to provide it in powder form. The method of filtration, concentration or drying is not particularly limited and can be carried out by a conventional method.

The fragrant wood fractions can be isolated from the above-mentioned hot-water extract of Hwangchu-kaku. The fractions can be obtained by fractionating the extract of Hwangjak tree using fractional solvent of at least one selected from the group consisting of hexane, chloroform, ethyl acetate and butanol. Preferably, the Fusarium oxysaccharide fraction of the present invention may be a fraction obtained by sequentially transferring the Fusarium oxysporum extract to solvent using hexane, chloroform, ethyl acetate and butanol. In detail, the hot water extract of Huangchu tree was suspended in water, transferred to a primary solvent with hexane, separated into a water layer and a hexane layer, a secondary solvent was transferred to a water fraction from which the hexane soluble component had been removed with chloroform to obtain a water layer and a chloroform layer , And the water fraction from which chloroform soluble component was removed was subjected to a tertiary solvent transfer with ethyl acetate to separate into a water layer and an ethyl acetate layer. To the water fraction from which the ethyl acetate soluble component had been removed, a fourth solvent was passed through n-butanol And fractions having different degrees of polarity can be obtained. The fragments of the woody wood of the present invention can contain fractions of hexane, chloroform, ethyl acetate, butanol and water obtained by transferring primary, secondary, tertiary and quaternary solvents have. More preferably, the Fusarium oxysulfur fraction of the present invention may be an ethyl acetate fraction obtained by sequentially subjecting a Fusarium oxysporum extract to a solvent using hexane, chloroform, and ethyl acetate as a fraction solvent. The separated fraction may be provided in the form of a liquid or solid powder having a controlled concentration by removing the solvent through filtration, concentration or drying. Preferably, in order to increase the concentration of the useful component and improve the shelf life, it is preferable to concentrate and freeze-dry to provide it in powder form. The method of filtration, concentration or drying is not particularly limited and can be carried out by a conventional method.

The hydrothermal extract or the fractions of Fusarium oxyspora prepared according to the above production method can increase or enhance the secretion and activity of the male hormone testosterone. The food composition of the present invention can be used to prevent or ameliorate male menopausal syndrome caused by decreased secretion of testosterone by containing hot water extract of Hwangchuok tree or fragrant woody fraction prepared according to the above production method. In the present invention, male menopausal syndrome includes male-menopausal-induced syndromes, male-menopausal disorders, and male-menopausal-related diseases. For example, there are hypothyroidism, mood changes due to hormonal changes, sleep disturbances, fatigue, muscle weakness, decreased exercise capacity, decreased strength, decreased body weight and muscle mass, increased visceral fat, sexual dysfunction, decreased cognitive abilities, and depression But is not limited thereto. The male menopausal syndrome may be induced by aging, or induced by chemical or surgical castration. The male menopausal syndrome may be one induced by decreased secretion of male hormone.

If desired, the food composition of the present invention may further comprise ingredients that are conventionally added in food preparation. The ingredients commonly added during the manufacture of the food may be at least one selected from the group consisting of pharmaceutically acceptable food-aid additives, carriers, excipients and diluents, but are not limited thereto. For example, there may be mentioned nutrients, vitamins, minerals (electrolytes), flavors, coloring agents, thickening agents, pectic acid, pectate salts, alginic acid, alginates, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, , Carbonating agent, pulp, and the like, but is not limited thereto. If desired, the food composition of the present invention may be prepared into any one of formulations selected from the group consisting of granules, powders, tablets, capsules, liquid solutions and rings. Through this, convenience for ingestion can be improved.

The composition of the present invention comprising hot-water extract of Huangchu tree or fractions of Huangchu wood can be provided as a food additive. The form of the food additive may be in the form of a granule, a powder, a tablet, a solution in a capsule liquid phase, and a ring form.

The composition of the present invention comprising hot-water extract of Hwangbuk tree or fragrant wood fractions can be produced as a functional food. The functional food refers to a food prepared by adding hot water extract of Huangchu tree or fractions of Huangchu tree to a food material, or a food prepared by capsules, powder or suspension of Huangchu hot water extract or Huangchu wood fraction, And include health foods in the usual sense that bring about certain effects. Examples of the food include drink, meat, sausage, bread, biscuit, rice cake, chocolate, candy, snack, confection, pizza, noodle, gum, ice cream, soup, beverage, tea, vitamin complex But is not limited thereto. The form of the food may be in the form of a powder, a granule, a tablet, a capsule, a liquid or a drink.

In the present invention, the content of the hotchprimer extract or the yellowish chestnut fraction may be 1 to 99% by weight, preferably 10 to 50% by weight, . In the present invention, the effective amount of the extract of Hwangbok tree or the extract of Hwacheon-shin wood, which is expected to prevent or ameliorate menopausal symptoms, may be 0.01 mg / kg to 10 g / kg, preferably 1 mg / kg to 1 g / kg.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail with reference to the following examples. It will be apparent to those skilled in the art that these embodiments are illustrative of the present invention and are not intended to limit the scope of the appended claims and that various changes and modifications may be made to the embodiments within the scope and spirit of the invention , And it is natural that such variations and modifications fall within the scope of the appended claims.

Example

≪ Preparation of Hot Water Extract of Yellowtail &

The leaves, branches and roots were separated and dried to remove moisture. Each part of the water - removed Hwangchu - wood was uniformly pulverized and powdered, and water was added to the Hwangchu - wood powder at 20 times volume and extracted at 120 ℃ for 4 hours. After filtration using a filter paper, the supernatant was decompressed and concentrated, and then lyophilized to obtain a hydrothermal extract.

≪ Preparation of fragrant wood fractions >

Distilled water and hexane were added in a volume ratio of 1: 1 by volume to 200 g of the dried Hwangchu tree hydrothermal extract, and the water layer and the hexane layer were separated into a first fraction and a hexane layer. The hexane layer was separated and chloroform was added in a volume ratio of 1: 1 to the remaining water layer. When the water layer and the chloroform layer were separated, a second fraction was obtained by a method of obtaining a chloroform layer. The chloroform layer was separated, and ethyl acetate was added to the remaining water layer in a volume ratio of 1: 1. When the water layer and ethyl acetate layer were separated, a third fraction was obtained by a method of obtaining an ethyl acetate layer. The ethylacetate layer was separated, and butanol was added to the remaining water layer in a volume ratio of 1: 1, followed by separation into a water layer and a butanol layer, followed by the fourth step of obtaining each layer. Each of the fractions obtained above was evaporated and concentrated to obtain hexane fraction, chloroform fraction, ethyl acetate fraction, butanol fraction and water fraction of Hwanghaek tree having different degrees of polarity.

<Measurement of the increase of testosterone secretion in the blood of Hwangchulchae hydrothermal extract>

The increase of male hormone secretion in the in vivo level of hydrothermal extract of P. japonicus was confirmed by using male rats of old age. The experimental group was divided into two groups. One group was Sprague-Dawley male rats aged 6 months, and the other group was a control group. Respectively. The experimental animals were kept in a temperature of 23 ± 1 ℃, humidity of 45 ± 5%, 12 hours of light intensity, and water and diet were freely consumed.

The experimental group was divided into three groups, and each group was orally administered the extract of Hwangchu - kogyo with different concentrations. In the experimental group, the extracts of Hwangjak tree were dissolved in distilled water at a concentration of 50, 100 or 200 mg / kg and then administered orally at the same time of day. Oral administration of the same amount of distilled water was performed in the control group. After oral administration and fasting for 4 weeks (28 days), blood was collected for the last oral administration. The collected blood was centrifuged at 4000 rpm for 10 minutes to separate the serum. Testosterone ELISA kit (ADI-900-065, ENZO Life Sciences, U.S.A) was used to measure the testosterone content in the separated serum. The results are shown in FIG.

In Fig. 1, the measured results were expressed as mean and standard error. Statistical significance was evaluated statistically by using Student's t-test for p <0.05 (p <0.05). This is also applied to Figs. 2 to 9 described below. In FIG. 1, the control group was denoted by control, and the experimental group was denoted by DM 50, DM100, and DM200, respectively. The number of each test group means the concentration (unit mg / kg) This is also applied to FIGS. 2 to 5 as follows.

Referring to FIG. 1, blood testosterone levels were significantly increased in all the experimental groups administered orally for 4 weeks (p> 0.05, p <0.01) compared to the control group. Through this, it can be confirmed that the hydrothermal extract of Huangchu tree increased testosterone secretion in the body.

<Measurement of inhibitory effect of SHBG on hydrothermal extract of Hwangchulchae>

The SHBG kit (Cloud-Clone Corp., USA) was used for the control and experimental group sera from the experiment of measuring the effect of increasing the testosterone secretion amount of the hot-water extract of Hwangchulchae, Was used to measure the SHBG content in blood and the results are shown in Fig.

Referring to FIG. 2, the SHBG content in blood was decreased in all the experimental groups administered orally for 4 weeks, compared with the control group (p <0.05 and p <0.01). Hydrothermal extract of Hwangchuang can increase the testosterone activity by decreasing SHBG concentration in the body.

<Determination of FSH and LH secretion increase effect of DHW extract>

In order to confirm the effect of the hydrothermal extract of Hwangchulchae on the increase and decrease of the gonadotropin, serum FSH and LH contents of the control and experimental groups isolated from the above test of " And the results are shown in Fig.

3, the serum FSH and LH contents were increased in a dose-dependent manner in all the experimental groups administered orally for 4 weeks, compared with the control group. Hwangchu tree hydrothermal extract promotes the secretion of gonadotropin in the body, induces differentiation and maturation of sperm, promotes testosterone secretion, and alleviates symptoms of diminished vigor due to male menopausal syndrome.

<Measurement of DHEA-s secretion increase effect of DHW extract>

In order to confirm the effect of DHEA-s on DHEA-s secretion, DHEA-s kit (Fine Biological Technology) was used for the control and experimental group sera isolated from the experiment of measuring the increase of testosterone secretion amount of the DHEA extract. , Hubei), and the results are shown in FIG.

Referring to FIG. 4, DHEA-s content in blood was increased in a dose-dependent manner in all experimental groups administered orally for 4 weeks, compared with the control group (p <0.05).

<Measurement of fatigue, muscle weakness and exercise capacity improvement effect of hot water extract of Hwangchil tree>

The fatigue, muscle weakness and exercise capacity of Hwangchilga were investigated using a treadmill exercise machine. Control and experimental groups were orally administered with distilled water or hot water extract of Hwangchuok tree in the same manner as in the experiment for measuring the increase of testosterone secretion amount of the hot water extract of Hwangchuok tree. The rotational speed of the rotating roll of the treadmill exercise device was set to 20 rpm, and the angle was inclined toward the electric stimulating plate at an angle of 20 degrees with the ground. On the last day of the administration, adaptive training was given for 10 minutes. After 24 hours, the rats were put on a treadmill exercise machine rotating at the same speed, and the exercise time was set up to the limit point where the rats could not run anymore, The anti - fatigue activity of the hot - water extract was measured. The measured results are shown in Fig.

5, the treadmill exercise time was significantly increased in all experimental groups administered orally for 4 weeks, compared with the control group (p <0.05). The results showed that the extract of Hwangchulchae hydrothermal extract had an improvement effect on the fatigue, muscle weakness and decrease in exercise capacity due to menopausal period.

<Cytotoxicity measurement of hot-water extract of Hwangchil tree>

The cytotoxicity test was conducted in order to confirm that the extract of Huangchu tree hydrothermal extract of the present invention does not show toxicity to cells and to determine an appropriate treatment concentration capable of effecting induction without inducing apoptosis. The cytotoxicity of the extract of Hwangchulchae was determined in TM3 cell line, which is a Lydia cell which secretes testosterone derived from rat testis and a male hormone. TM3 cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin / streptomycin at 37 ° C and 5% CO ₂ .

TM3 cells were cultured in a 96-well plate at a concentration of 1 × 10 ⁵ cells / mL at 37 ° C and 5% CO ₂ . The extracts were treated at 0, 1, 3, 10, 30 and 100 μg / mL for 24 h. MTT solution was added. After 3 hours, the supernatant was removed, and 100 μL of DMSO was added to each well. Absorbance was measured at 540 nm using an ELISA reader. The results are shown in FIG.

Referring to FIG. 6, it can be confirmed that the extract of Hwangcholga extract does not show cytotoxicity against TM3 cells at all concentrations treated.

<Effect of hydrothermal extract of Hwangchu tree on the increase of testosterone secretion in TM3 cells>

The increase of male hormone secretion at the in vitro level of hydrothermal extract of Hwangchulchae was confirmed by using TM3 cells, Lydia cells of mouse. TM3 cells were inoculated into a 24-well plate at a concentration of 1 × 10 ⁵ cells / mL and cultured in DMEM supplemented with 10% FBS at 37 ° C and 5% CO ₂ . The testosterone ELISA kit (ADI-900-065, ENZO Life Sciences, Inc.) was used to collect the supernatant after 24 hours of incubation at 0, 1, 3, 10, 30 and 100 μg / , USA) was used to measure the testosterone content, and the results are shown in FIG.

Referring to FIG. 7, it can be seen that testosterone secretion was significantly increased in all the experimental groups treated with the extract of Hwangchu-kogyo (FIG. 7) in a concentration-dependent manner (p <0.05).

<Determination of the effect of hydrothermal extract of Hwangchulchae on the increase of testosterone secretion in TM3 cells under hydrogen peroxide induction condition>

TM3 cells were inoculated into a 24-well plate at a concentration of 1 × 10 ⁵ cells / mL and cultured in DMEM supplemented with 10% FBS at 37 ° C and 5% CO ₂ . The extracts were treated at 0, 1, 3, 10, 30, and 100 μg / mL concentration, and H ₂ O ₂ was treated at ₂₀₀ μM for 24 hours. The testosterone content was measured using a testosterone ELISA kit (ADI-900-065, ENZO Life Sciences, USA) and the results are shown in FIG.

Referring to FIG. 8, the hydrogen peroxide testosterone concentration of TM3 cell cytotoxicity induced was decreased testosterone secretion is significant compared to the group (control) was not treated _{H 2 O 2 (p <0.05} ), H 2 O 2 and hwangchil (P <0.05, p <0.01 and p <0.001), respectively, in the case of simultaneous treatment of hot water extracts of trees.

<Determination of Effect of Foliar Trees on Increase of Testosterone Secretion in TM3 Cells under Hydrogen Peroxide Induction Condition>

TM3 cells were inoculated into a 24-well plate at a concentration of 1 × 10 ⁵ cells / mL and cultured in DMEM supplemented with 10% FBS at 37 ° C and 5% CO ₂ . The hexane fraction, the chloroform fraction, the ethylacetate fraction, the butanol fraction and the water fraction of Hwangchulchu were classified into 0, 1, 3 and 10 μg / mL by each fraction and concentration and treated with ₂₀₀ μM H ₂ O ₂ , And then the supernatant was taken and the testosterone content was measured using a Testosterone ELISA kit (ADI-900-065, ENZO Life Sciences, USA). The results are shown in FIG. 9 . For reference, in FIG. 9, each fraction was indicated as a corresponding solvent layer. For example, the hexane layer in Figure 9 refers to the hexane fraction and the water layer refers to the water fraction remaining after the fourth fraction.

9, the testosterone concentration of TM3 cells induced by H ₂ O ₂ cytotoxicity was significantly lower than that of control without H ₂ O ₂ , and the testosterone secretion was significantly decreased, while the H ₂ O ₂ and Houttuynia chinensis fractions were simultaneously treated (P <0.01 and p <0.001), respectively. Ethyl acetate (EtOAc) fractions were found to be more effective than other fractions of Hwanyeong tree fractions.

Claims (8)

Dendropanax morbifera Lev . ) A functional food composition for improving male menopausal symptoms selected from fatigue, muscle weakness or athletic ability deprivation containing hot water or hot pepper extract fraction as an active ingredient.
The method according to claim 1,
Wherein the water extract of Huangchu tree is prepared by adding 5 to 30 times volume of water to Huaculi tree and extracting at 100 to 130 ° C for 3 to 6 hours.
The method according to claim 1,
Wherein the extract is obtained by fractionating at least one member selected from the group consisting of hexane, chloroform, ethyl acetate and butanol with a fraction solvent to obtain a functional food composition for improving the menopausal symptom.
The method according to claim 1,
Wherein the fragrant woody fraction is an ethyl acetate fraction obtained by successively fractionating the extract of Fusarium oxysporum horticulture with hexane, chloroform and ethyl acetate.
The method according to claim 1,
Wherein the composition further comprises at least one selected from the group consisting of a pharmaceutically acceptable food-aid additive, a carrier, an excipient and a diluent.
The method according to claim 1,
Wherein the active ingredient is at least one selected from the group consisting of granules, powders, tablets, capsules, liquid solutions and rings.
A functional food additive for improving menopausal symptom, which comprises the functional food composition according to any one of claims 1 to 6.
A health functional food for improving menopausal symptoms characterized by comprising the functional food composition of any one of claims 1 to 6.

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