KR101726410B1 - Composition preventing or treating andropause comprising dendropanax morbifera lev. fermentated extract - Google Patents

Abstract

The present invention relates to a composition for preventing and treating male menopausal period syndrome including a fermented extract of Fusarium oxysporum, morbifera LEV.); Adding a polar solvent to the dried woody wood to obtain a woodchuck extract; And a method for preventing, ameliorating or treating male menopausal symptoms which can increase testosterone secretion in the body by including the fermented extract of Fusarium oxysporum prepared by the method comprising lactic acid fermentation by inoculating lactic acid bacteria to the extract of Ganoderma lucidum .

Description

TECHNICAL FIELD The present invention relates to a composition for prevention and treatment of menopausal symptoms syndrome, which comprises fermented extract of Hwangchu-mung tree. FERMENTATED EXTRACT}

The present invention relates to a composition for the prevention and treatment of male menopausal syndrome which comprises fermented extract of Fusarium oxysporum, and more particularly to a composition for improving menopausal symptoms of Fusarium oxysulfurum fermented extract which increases body testosterone secretion, ≪ / RTI > pharmaceuticals and health supplements.

Women become menopausal and female hormones are rapidly decreased, resulting in several visible changes, which are called menopause. The term "normal menopausal period" has been used only in women, but in recent years, the concept of male menopausal syndrome is emerging as a result of the gradual decrease of the male hormone in the body due to aging. The symptoms of real male menopause are similar to those arising from chemical or surgical castration for the treatment of prostate adenocarcinoma. The terms termed "late-onset hypogonadism (LOH)", "age-induced sexual dysfunction syndrome (LOH)" and " testosterone deciency syndrome, TDS). Andropause is a clinical and biochemical syndrome associated with increased age, defined as a typical symptom and a deficiency of serum testosterone levels, which can negatively affect the function of various organs. Symptoms include decreased sexual desire and erectile function, nighttime depression, decreased cognitive ability, decreased fatigue, weight and muscle mass, and atrophy of the skin.

It is argued that the use of male hormone replacement therapy in a manner similar to the use of estrogen replacement therapy in postmenopausal women. According to one study, significant improvement in the effects of treating male menopausal patients with 40 x 2 mg / day of testosterone undecanoate has been reported. However, administration of male hormones has also been associated with the risk of adversely affecting liver, lipid conditions, cardiovascular and prostate diseases. In addition, in terms of adenomas and adenocarcinomas, it is known that about 30% of patients with symptoms attributable to male menopause can not receive male hormone replacement therapy. Therefore, there is a need for the development of new therapeutic agents useful in the prevention and treatment of male menopausal syndromes caused by aging, or chemical or surgical castration, which do not provide disadvantages of hormone replacement therapy.

The use of natural product-derived pharmaceutical compositions in the field of drug development is increasing worldwide. Dendropanax morbifera Lev.) Is an evergreen broad-leaved arboreous tree native to the southern coast of Korea and Jeju-do. It is a species that does not fall into winter when it blooms in the winter. When it bruises the bark, yellowish resinous liquid is called yellowish lacquer. Huangchil has been used as a rare paint for the emperor's armor, helmet, and other metal ornaments since the Three Kingdoms period. It has been used in the Goryeo period, which was written in the Goryeo period, the Guilin variety in China, And it is said that it was a special product of Baekje which was before it, and it remains in the passage of the book department of the Tang history book. It is said that it is effective for burning removal, eye quality and burn treatment, leprosy and harmless.

KR 1014432300000 B1

It is an object of the present invention to provide a composition for preventing, ameliorating or treating male menopausal syndrome, which comprises a fermented extract of Yellow Grass Tree.

It is another object of the present invention to provide a pharmaceutical preparation and a functional food for preventing, ameliorating or treating male menopausal syndrome, which comprises an extract of Fusarium oxysporum as an active ingredient.

According to one aspect of the present invention, there is provided a dendropanax morbifera A composition for preventing or treating male menopausal syndrome comprising a fermented extract of LEV. Adding a polar solvent to the dried woody wood to obtain a woodchuck extract; And a step of fermenting lactic acid bacteria by inoculating lactic acid bacterium to the extract of Hwigae mackerel. The composition for preventing or treating male menopausal syndrome is provided.

The whitish wood may be at least one selected from the group consisting of leaves and branches of perennials.

In addition, the lactic acid bacteria may be lactic acid bacteria belonging to the genus Lactobacillus .

In addition, the sugar concentration of the extract of Hwanyeong cherry tree which fermented with lactic acid may be 1 to 10 Brix.

The lactic acid fermentation may be carried out at 30 to 40 DEG C for 24 to 48 hours.

In addition, the Fusarium oxysulfurum fermented extract may increase testosterone secretion in the body.

According to another aspect of the present invention, there is provided a dendropanax morbifera The present invention relates to a pharmaceutical preparation for the prevention or treatment of male menopausal syndrome comprising a fermented extract, Adding a polar solvent to the dried woody wood to obtain a woodchuck extract; And a step of fermenting lactic acid bacteria by inoculating lactic acid bacterium to the extract of Hwigae mackerel. The pharmaceutical preparation for preventing or treating male menopausal syndrome is provided.

According to another aspect of the present invention, there is provided a dendropanax morbifera The present invention relates to a functional food for preventing or ameliorating male menopausal syndrome comprising a fermented extract, Adding a polar solvent to the dried woody wood to obtain a woodchuck extract; And a step of fermenting lactic acid bacteria by inoculating lactic acid bacteria in the extract of Hwigaechuki, and thereby producing a functional food for preventing or ameliorating male menopausal syndrome.

The composition comprising the Fusarium oxysulfurum L. extract according to one aspect of the present invention can increase the level of testosterone in the body. This can be used in the medical and food industries for the purpose of preventing, ameliorating and treating male menopausal disorders and male diseases, as it can alleviate the symptoms caused by menopausal symptoms, including muscle weakness and fatigue. have.

In addition, the composition comprising the Fusarium oxysporum fungus extract according to one aspect of the present invention does not induce apoptosis of normal cells and is thus safe for human body, and thus can be used as safe medicines or food additives.

In addition, the composition comprising the extract of Fusarium oxysporum according to one aspect of the present invention can reduce toxicity and side effects when compared with a composition containing an existing chemical substance by containing a natural plant-derived extract.

FIG. 1 is a graph showing that the fermented extract of Huangchuang tree of the present invention does not show cytotoxicity in TM3 cells,
FIG. 2 is a graph showing the effect of the extract of D. torrillum extract of the present invention on the increase of testosterone secretion in TM3 cells,
FIG. 3 is a graph showing that the extract of D. perilla of the present invention has an effect of increasing testosterone secretion in an oxidative stress environment induced by hydrogen peroxide in TM3 cells.

The invention hwangchil tree (Dendropanax morbifera LEV.); Adding a polar solvent to the dried woody wood to obtain a woodchuck extract; And a method of fermenting lactic acid bacteria by inoculating lactic acid bacteria to the extract of Hwigae mackerel. The composition for preventing, improving or treating male menopausal symptoms according to claim 1, Functional food.

Hereinafter, the present invention will be described in more detail.

The composition of the present invention contains a fermented extract of Hwangchujang as an active ingredient. The lactic acid fermented extract can be improved in functionality and flavonoid content by the lactic acid fermentation.

In order to prepare the fermented extract of Hwangchujang, the Hwangchujang is dried first. The drying method is not particularly limited, and a usual method can be used. By drying the yellowtail, it is possible to improve the weakness of the yellowtail. If desired, the woodblock can be cut and crushed before or after drying. It is preferable that at least one selected from the group consisting of leaves and branches of perennials is selected.

Next, a polar solvent is added to the dried woody wood to obtain a woodywood extract. There is no particular limitation on the method for separating the Hokutogi (Japanese White Lucy) extract, and a method commonly used in the art can be used for producing the extract. For example, a method using an extraction device such as hot water extraction, immersion extraction, reflux cooling extraction, supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction and ultrasonic extraction, or a method using an adsorption resin containing XAD and HP-20 Method, but the present invention is not limited thereto. Preferably, the extraction solvent can be treated to extract hot water.

In the present invention, a polar solvent is used as the extraction solvent. It is preferable that water is selected as the polar solvent. When extracting the extractant by treating the extracting solvent, the extracting solvent may be added at a ratio of 1 to 100 times the dry weight of the whole wood. Preferably, it is added in an amount of 5 to 20 times the dry weight of the whole wood.

The temperature at which the extract is separated by treating the extraction solvent can be adjusted according to the extraction method. For example, from 40 to 120 캜. The extract may be extracted at the above temperature for 1 to 72 hours to isolate the extract. Preferably, the extract is extracted at 100 DEG C for 2 to 12 hours to isolate the extract. More preferably, the extract is extracted at 100 DEG C for 3 to 7 hours to isolate the extract. The separated extract can then be adjusted in concentration by removing the solvent through filtration, concentration or drying. The method of filtration, concentration or drying is not particularly limited and can be carried out by a conventional method.

Next, the lactic acid bacteria are inoculated to the extract of Hwangchu-myeon to ferment lactic acid. Preferably, the lactic acid bacterium is selected from an edible lactic acid bacterium belonging to the genus Lactobacillus . More preferably, it is Lactobacillus brevis or Lactobacillus plantarum . The inoculation is carried out by culturing lactic acid bacteria in a solid medium and culturing the colonies in a liquid medium at 37 DEG C for 24 hours to obtain a culture medium. Then, the culture medium is added to the Hwigaechii extract at a concentration of 3 to 8% (v / v) .

The saccharide concentration of the woodchuck extract to be fermented with lactic acid may be 1 to 10 Brix. When the sugar concentration of the extract is less than 1 brix, the fermentation efficiency is not good due to the lack of glycyrrhizin and the yield of the fermented extract is higher than 10 brix. If the sugar concentration is more than 10 brix, the activity of lactic acid bacteria is lowered, There is a possibility that the yield of fermented extract of Huchilchia wood is reduced. The saccharide concentration of the Hwigae extract can be controlled by concentrating or diluting the Hwigae extract.

The lactic acid fermentation can be carried out at a growth temperature of the selected lactic acid bacteria at 30 to 40 DEG C for 24 to 48 hours. Preferably, when Lactobacillus brevis or Lactobacillus plantarum is used as lactic acid bacteria, it may be carried out at 37 DEG C for 24 to 48 hours. When the fermentation time of lactic acid is less than 24 hours, the yield of the fermented extract of Ganoderma lucidum is reduced, and the effect of the composition of the present invention containing the fermented extract is reduced. If the fermentation time exceeds 48 hours, over fermentation may occur.

If necessary, lactic acid fermented yellowtail oil fermented extracts can be post-processed. The post-processing may include concentration, drying, etc., and the fermented extract of Huangchu tree may be provided in the form of liquid or solid powder according to the post-processing. Preferably, in order to increase the concentration of the useful component and improve the shelf life, it is preferable to concentrate and freeze-dry to provide it in powder form.

The fermented extract of Fusarium oxysporum prepared according to the above-described production method can increase the secretion of testosterone in the body. The composition of the present invention can be used for preventing, ameliorating or treating male menopausal syndrome caused by decreased secretion of testosterone by including fermented extract of Huaxia tree prepared according to the above production method. In the present invention, male menopausal syndrome includes male-menopausal-induced syndromes, male-menopausal disorders, and male-menopausal-related diseases. Examples include, but are not limited to, mood changes due to hormonal changes, sleep disorders, weight and muscle mass loss, muscle weakness, visceral fat increase, sexual dysfunction, cognitive impairment, fatigue and depression. The male menopausal syndrome may be induced by aging, or induced by chemical or surgical castration.

The composition comprising the Fusarium oxysulfurum fermented extract of the present invention can be prepared as a pharmaceutical preparation. The pharmaceutical preparations of the present invention can be prepared by using at least one selected from the group consisting of pharmaceutically acceptable carriers, diluents and excipients according to a method which can be easily carried out by those having ordinary skill in the art to which the present invention belongs. Or by formulating it into a unit dose form or by inserting it into a multi-dose container. The formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules. The formulation may further comprise a dispersant or stabilizer.

If desired, the pharmaceutical preparation may further comprise a pharmaceutically acceptable carrier. Such pharmaceutically acceptable carriers are those conventionally used in the field of the present invention and include for example lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, But are not limited to, crystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

If desired, the pharmaceutical preparations of the present invention may further contain additives known in the art in addition to the above components. For example, it may further include at least one selected from the group consisting of a lubricant, a wetting agent, a sweetener, a flavoring agent, an emulsifying agent, a suspending agent, and a preservative agent, but is not limited thereto.

The pharmaceutical preparation of the present invention can be administered orally or parenterally. In case of parenteral administration, it can be administered by skin application, rectal injection, intravenous injection, subcutaneous injection, muscle injection, intraperitoneal injection and transdermal administration. A suitable dosage of the pharmaceutical composition of the present invention may be controlled by such factors as the formulation method, the manner of administration, the age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate and responsiveness of the patient And the ordinarily skilled physician can readily determine and prescribe dosages effective for the desired treatment or prophylaxis.

The composition of the present invention comprising the fermented extract of Fusarium oxysporum may be produced as a functional food. The functional food is a food prepared by adding a fermented extract of Huangchu tree to a food material or a fermented extract of Huangchu tree made of a capsule, a powder, a suspension, etc., and a food having the usual meaning Of healthy foods. Examples of the food include drink, meat, sausage, bread, biscuit, rice cake, chocolate, candy, snack, confection, pizza, noodle, gum, ice cream, soup, beverage, tea, vitamin complex But is not limited thereto.

If necessary, the functional food of the present invention may further comprise components that are ordinarily added in food production. For example, there may be mentioned nutrients, vitamins, minerals (electrolytes), flavorings, colorants, thickeners, pectic acids, pectate salts, alginic acid, alginates, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, , Carbonating agent, pulp, and the like, but is not limited thereto.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail with reference to the following examples. It will be apparent to those skilled in the art that these embodiments are illustrative of the present invention and are not intended to limit the scope of the appended claims and that various changes and modifications may be made to the embodiments within the scope and spirit of the invention , And it is natural that such variations and modifications fall within the scope of the appended claims.

Example

≪ Preparation of Fermented Extracts of Wild Mill Tree &

Leaves and branches of Hwangchil were separated and dried by natural drying. 20 times volume of water was added to the dried woody leaves and branches, and the mixture was subjected to hot extraction at 100 ° C for 4 hours. The extract was filtered through a filter paper and concentrated to a concentration of 30 Bricks. And then diluted with distilled water to a concentration of 3 to 6 Bricks per liter for use in lactic acid fermentation. Samples of the same amount of Ullung wood extract were prepared and fermented using lactobacillus Lactobacillus brevis and Lactobacillus plantarum , respectively. The culture broth obtained by culturing the single colony in an MRS liquid medium at 37 DEG C for 24 hours was added to the Hwacheon-mulberry extract at a concentration of 5% (v / v) ). After lactic acid fermentation at 37 ° C for 40 hours, the mixture was concentrated under reduced pressure using a large rotary evaporator and lyophilized to give a fermented extract of Hwangjak tree. In the following examples, the fermented extract of Fusarium oxysporum fungus fermented with Lactobacillus brevis was fermented by using DMB and Lactobacillus plantarum , and the fermented extract of Fusarium oxysporum was named DMP.

<Identification of Cytotoxicity of Fermented Extracts of Yellow Grass Tree>

Prior to confirming the efficacy of the Fusarium oxysporum fermentation extract of the present invention, a cytotoxicity test was carried out to determine an appropriate concentration that does not show toxicity to cells and can produce an effect without inducing apoptosis. Cytotoxicity of Hwangchu tree fermented extract was measured in TM3 cell line which is derived from rat testis and ladig cell which secretes testosterone which is male hormone. TM3 cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin / streptomycin at 37 ° C and 5% CO ₂ .

TM3 cells were cultured in a 96-well plate at a concentration of 1 × 10 ⁵ cells / ml at 37 ° C and 5% CO ₂ . Fermented extracts of Hwangchu - tree were treated at 1, 3, 10, 30 and 100 ㎍ / ㎖ in each well and incubated for 24 hours. MTT solution was added. After 3 hours, the supernatant was removed. 100 μl of DMSO was added to each well. Absorbance was measured at 540 nm using an ELISA reader. The results are shown in FIG. For reference, all the measured results shown in FIGS. 1 to 3 are expressed as mean and standard deviation, and the statistical significance test was performed using Student's t-test analysis, in which the p value was less than 0.05 (p <0.05) .

Referring to FIG. 1, it can be confirmed that the fermented extract of Huangchu tree does not show cytotoxicity to TM3 cells at all concentrations treated.

<Measurement of testosterone increase effect of fermented extract of Wuchul tree>

TM3 cells were cultured in DMEM supplemented with 10% FBS at 37 ° C and 5% CO ₂ at a concentration of 1 × 10 ⁵ cells / ml on a 24-well plate. Fermented extracts of Hwangchu - tree were treated at 1, 3, 10, 30 and 100 ㎍ / ㎖ in each well and incubated for 24 hours. Testosterone ELISA kit (ADI-900-065, ENZO Life Sciences, USA) was used to measure the testosterone content. The results are shown in FIG. As a control, TM3 cells cultured under the same conditions without any addition were used.

Referring to FIG. 2, it can be seen that the testosterone level was significantly increased in a concentration-dependent manner when the fermented extract of Fusarium oxysporum was treated. Especially, when DMP fermented with Lactobacillus plantarum was treated with 100 ㎍ / ㎖, testosterone level was significantly increased (p <0.01). These results indicate that the extract of Fermented Green mulberry tree increased the secretion of testosterone in TM3 cells. The fermented extract of Huangchu tree of the present invention can increase the testosterone secretion of TM3 cells and increase the level of testosterone in the body, thereby showing the effect of alleviating and treating male menopausal syndrome.

<Measurement of testosterone increase effect of Fermented Extract of Ganoderma lucidum in hydrogen peroxide induction condition>

TM3 cells were cultured in a DMEM medium supplemented with 10% FBS at 37 ° C and 5% CO ₂ at a concentration of 1 × 10 ⁵ cells / ml on a 24-well plate. The fermented extracts of Hwangjak tree were treated at 1, 3, 10, 30 and 100 ㎍ / ㎖ in each well and H ₂ O ₂ was treated at ₂₀₀ μM for 24 hours. Testosterone ELISA kit (ADI-900-065, ENZO Life Sciences, USA) was used to measure the testosterone content. The results are shown in FIG. TM3 cells cultured by treating with H ₂ O _{2 at a} concentration of ₂₀₀ μM were used as a control, and TM3 cells cultured under the same conditions without any addition or treatment were used as a comparative example. In FIG. 3, the comparative example is denoted by N.

Referring to FIG. 3, it can be seen that the testosterone level was significantly increased in a concentration-dependent manner even in the oxidative stress environment induced by hydrogen peroxide when the fermented extract of Hwanyeongsan was treated. When DMB fermented with Lactobacillus brevis was treated with DMB (p <0.05) and Lactobacillus plantarum fermented with DMP (p <0.01) Testosterone levels were significantly increased in a concentration-dependent manner compared to the control group. In particular, when DMP was treated at a concentration of 100 μg / ml, the testosterone level was increased compared to the comparative example. The ferulic extract of Huchulchia japonica of the present invention can increase the testosterone secretion of TM3 cells even in an environment where oxidative stress is induced, thereby increasing the level of testosterone in the body, thereby alleviating and treating male menopausal syndrome.

Claims (8)

A composition for prevention or treatment of male menopausal syndrome comprising a fermented extract of Dendropanax morbifera LEV.
The above yellowtail tree fermentation extract
Drying the perennial tree;
Adding a polar solvent to the dried woody wood to obtain a woodchuck extract; And
Which comprises the step of fermenting lactic acid bacteria by inoculating lactic acid bacteria in the extract of Hwigae et al.
Wherein the lactic acid bacterium is Lactobacillus brevis . 2. The composition according to claim 1, wherein the lactic acid bacterium is Lactobacillus brevis .
The composition for preventing or treating male menopausal symptoms according to claim 1, wherein the perennial tree is at least one selected from the group consisting of leaves and branches of perennial tree.
delete The composition for preventing or treating male menopausal symptoms according to claim 1, wherein the lactic acid extract has a sugar concentration of 1 to 10 Brix.
The composition for preventing or treating male menopausal symptoms according to claim 1, wherein the lactic acid fermentation is performed at 30 to 40 DEG C for 24 to 48 hours.
The composition for preventing or treating male menopausal symptoms according to claim 1, wherein the fermented extract is a testosterone secretagogue.
A pharmaceutical preparation for the prevention or treatment of male menopausal syndrome comprising a fermented extract of Dendropanax morbifera LEV.
The above yellowtail tree fermentation extract
Drying the perennial tree;
Adding a polar solvent to the dried woody wood to obtain a woodchuck extract; And
Which comprises the step of fermenting lactic acid bacteria by inoculating lactic acid bacteria in the extract of Hwigae et al.
Wherein the lactic acid bacterium is Lactobacillus brevis . 2. The pharmaceutical composition according to claim 1, wherein the lactic acid bacterium is Lactobacillus brevis .
A functional food for the prevention or amelioration of male menopausal syndrome including fermented extract of Dendropanax morbifera LEV.
The above yellowtail tree fermentation extract
Drying the perennial tree;
Adding a polar solvent to the dried woody wood to obtain a woodchuck extract; And
Which comprises the step of fermenting lactic acid bacteria by inoculating lactic acid bacteria in the extract of Hwigae et al.
Wherein the lactic acid bacterium is Lactobacillus brevis . 2. The functional food for preventing or ameliorating male menopausal symptoms according to claim 1, wherein the lactic acid bacterium is Lactobacillus brevis .

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