KR100727396B1 - Composition comprising extract of Citrullus vulgaris bark and octacosanol - Google Patents

Abstract

The present invention relates to a composition containing a mixture of watermelon peel extract and octacosanol, the composition is effective in improving the liver function by reducing the cholesterol and triglyceride content in the body, increasing the concentration of creatine kinase in the blood and the concentration of lactic acid in the blood It has a stamina enhancing effect, and also has a blood sugar enhancing effect.

Description

Composition containing extract of Citrullus vulgaris bark and octacosanol}

1 shows a chromatogram of standard citrulline.

2 is a diagram showing a calibration curve showing the content of citrulline to the area of HPLC on standard citrulline.

Figure 3 shows a chromatogram of citrulline isolated from 80% ethanol extract of watermelon peel.

Figure 4 is a diagram showing the weight change of the rat ingested the composition of the present invention.

5 is a diagram showing the results of swimming experiments of rats ingesting the composition of the present invention for 2 weeks.

Figure 6 is a diagram showing the total cholesterol level between each group (group).

7 is a diagram showing triglyceride levels between groups.

8 is a diagram showing HDL-cholesterol levels between groups.

9 is a diagram illustrating a GOT value between groups.

10 is a diagram illustrating GPT values between groups.

11 is a diagram showing the total cholesterol level of serum in each group.

12 is a diagram showing triglyceride levels of serum of each group.

Figure 13 is a diagram showing the HDL-cholesterol level of serum in each group.

14 is a diagram showing the GOT level of serum of each group.

15 is a diagram showing the GPT level of serum of each group.

16 is a diagram showing creatine kinase levels in serum of each group.

17 is a diagram showing the inorganic phosphoric acid level of serum in each group.

18 is a diagram showing the lactic acid level of serum in each group.

19 is a diagram showing the glucose level of serum in each group.

The present invention relates to a composition containing a mixture of watermelon peel extract and octacosanol, and more particularly, to a composition for enhancing stamina, a composition for improving liver function, and a composition for lowering blood sugar.

The term stamina means stamina, stamina, endurance, and the like.

As the industry develops and becomes complex, many outsiders complain of fatigue. Fatigue refers to a state of deterioration of physical and mental functioning, physical or mental exhaustion or weakness that occurs with continuous and repeated mental and physical work. The case is called chronic fatigue. Frequently, when you feel tired, you may feel weak, tired, weak, and indifferent to other things.

About 80% of the causes of fatigue occur in a mental state called depression and anxiety, and 20% is known to be caused by physical abnormalities.

When fatigue occurs, the adrenal gland is stimulated by adrenal cortical stimulating hormones to increase weight, increase lipids, and reduce the spleen's weight due to a decrease in immune function. The adrenal glands secrete other steroid hormones in the blood, causing cholesterol, blood sugar, alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and aspartate aminotransferase (AST or GOT). Alanine aminotransferase (ALT or GPT).

In addition, fatigue occurs in the muscle itself, three reasons are being mentioned. The process of muscle contraction is seen as a process of excitation-contraction, which involves many ions in the cell membrane. Tired muscles have the first opinion that this excitement process is not done properly and the excitation is delayed, and as a result, calcium secretion is delayed so that sufficient contraction does not occur. Second, the lack of ATP causes muscle fatigue. Third, there is an opinion that an increase in hydrogen ions, inorganic phosphate (IP) and ADP generated during muscle contraction is one cause. The increase in hydrogen ions affects the acid-base equilibrium in the cell, slows glycolysis, interferes with the contraction process because it tries to bind troponin competitively with calcium, and calcium morphology This impedes resorption of the furnace and consequently lowers the secretion of calcium.

In general, physical methods such as sleep, rest, and exercise are used for prevention and treatment of fatigue, but they cannot be fundamentally treated.

In ancient times, such as Korea, China, etc., oriental medicines such as red ginseng, ginseng, honey, tofu, jujube, gojija and hong kyung cheon are used as herbal or folk remedies as ingredients for nourishing tonic to cure fatigue or weakness. In recent years, various types of health foods and medicines based on these ingredients have been developed and marketed as fatigue recovery and nourishing tonic drinks.

However, these herbal medicines and products using them can not find a big characteristic technology in terms of simply using the herbal extract that has been used as a traditional herbal medicine in the Orient. Therefore, there is a need for the development of a new fatigue recovery material which is low in cost.

Watermelon ( Citrullus vulgaris ) belongs to the family gourd and is native to Africa. It is also known as western, aquatic, Korean, and poems, and stems grow long, branching on the ground.

The flesh of the watermelon is more than 90% water, 7.9% of the juice contains sugar, a small amount of uric acid and arginine, the red pigment is lycopine and carotene. The pulp is yellow in addition to red. Seeds contain about 20% fat and more than 50% protein, which are then roasted and sprinkled with salt and used as an appetizer for Chinese cuisine.

 Watermelon has been used in folk medicine for systemic or other edema. The protein we eat breaks down into the body and becomes a urea, which is once again converted into urine. Watermelon has a special ingredient called citrulline, an amino acid, which helps diuretics because it helps the protein turn into urea and is excreted in the urine. This is why it is effective in kidney disease.

Citrulline (citrulline) is an amino acid represented by the following formula (1), which is present in large amounts in the seeds of gourd plants, and plays an important role as an intermediate in the ornithine circuit of urea production together with ornithine and arginine in vivo. do.

<Formula 1>

Octacosanol (octacosanol) is isolated from extracted, concentrated and purified oil from rice bran, tangerine, wheat germ, etc. and is a straight-chain aliphatic higher alcohol having a molecular formula of CH ₃ (CH ₂ ) ₂₆ CH ₂ OH.

It is known that octacosanol has the effect of improving physical strength, improving muscle function, and improving basic metabolism, and has already been announced by the Ministry of Health and Welfare as 24 health supplements, and its effects and effects are already generalized. It is ingredient listed.

The recommended daily intake of octacosanol is 4 mg, but it has been found to be stable at over 1200 mg / kg, the limit administered in acute toxicity studies in animals. In addition, it improves resistance to stress and improves metabolic rate, that is, energy burning rate, so it is good for relieving obesity by burning excess nutrients in the body, and relieves coldness due to heat generated by energy burning.

Therefore, the inventors of the present invention, while researching natural products to find a new fatigue recovery material, watermelon peel extract and octacosanol mixture reduces the body's cholesterol and triglyceride content to improve liver function, increase the concentration of creatine kinase in the blood and The present invention was completed by confirming that there is a stamina enhancer effect by decreasing the concentration, and that there is a hypoglycemic effect.

The present invention is to provide a health functional food composition for stamina enhancement containing a mixture of watermelon peel extract and octacosanol.

In addition, the present invention is to provide a composition for improving liver function containing a mixture of watermelon peel extract and octacosanol.

In addition, the present invention is to provide a blood sugar lowering composition containing a mixture of watermelon peel extract and octacosanol.

The present invention provides a health functional food composition for enhancing stamina containing a mixture of watermelon peel extract and octacosanol.

In addition, the present invention provides a composition for improving liver function, which contains a mixture of watermelon peel extract and octacosanol.

The present invention also provides a composition for lowering blood sugar, which contains a mixture of watermelon peel extract and octacosanol.

More preferably, the watermelon peel extract is extracted with ethanol, octacosanol is a mixture of 1% content, most preferably, the watermelon peel extract extracted with 80% ethanol and 1% content of octacosanol in a weight ratio of 4: 6 It is characterized by that.

Watermelon peel extract according to the present invention can be prepared as follows.

Remove the flesh of the watermelon, wash the skin thoroughly with water, and dry in the shade. The dried watermelon rind is placed in an extraction vessel, immersed in an appropriate amount of extraction solvent and extracted for a certain time at an appropriate temperature. The extract can be filtered and concentrated.

As the extraction solvent, a solvent selected from water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof may be used, preferably ethanol, and most preferably 80% ethanol.

The extraction method may be an extraction method such as bath extraction, reflux cooling extraction, ultrasonic extraction.

After the watermelon peel extract is obtained, solids may be removed using a filter paper, the suspension may be centrifuged, and the supernatant may be filtered under reduced pressure.

The filtered extract can be used by concentrating with a vacuum rotary concentrator.

The mixture of watermelon peel extract and octacosanol according to the present invention can be prepared by mixing watermelon peel extract prepared by the above method and octacosanol commercially available.

More preferably, the watermelon peel extract is extracted with 80% ethanol, the octacosanol is 1% content, watermelon peel extract and 1% octacosanol are mixed in a weight ratio of 4: 6.

The mixture of watermelon peel extract and octacosanol according to the present invention has superior stamina enhancing effect than when octacosanol, watermelon citrate or watermelon peel extract contained in watermelon extract was administered alone in an in vivo experiment using a rat as an experimental animal, Hepatic function and blood sugar lowering effect are shown. Specifically, it reduces liver and blood total cholesterol content and triglyceride content, increases the concentration of creatine kinase in the blood, reduces the concentration of lactic acid in the blood and decreases the blood glucose concentration.

The composition containing the mixture of watermelon peel extract and octacosanol of the present invention can be prepared as follows.

In the composition of the present invention, the mixture of watermelon peel extract and octacosanol is preferably contained in an amount of 10 to 100% by weight based on the total weight of the composition, but has a stamina enhancement effect, a liver function improvement effect and a blood sugar enhancing effect, and no toxicity, in the composition. It may be used in the above or below the range depending on the intended use.

The composition of the present invention may further contain at least one active ingredient exhibiting the same or similar function in addition to the mixture of the watermelon peel extract and octacosanol.

The composition of the present invention may be used in various forms such as tablets, drugs, powders, and when used as a beverage, may contain various flavors or natural carbohydrates as additional components, such as conventional drinks. The above-mentioned natural carbohydrates are glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin and aspartame, and the like can be used.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, And a carbonation agent used for the carbonated beverage. The proportion of such additives is not critical but is usually selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The composition of the present invention may be administered once to several times a day at a dose of 0.1 to 10 g / kg, which dose can be added or subtracted as necessary.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

Example: Sample Preparation

1-1: Preparation of Watermelon Peel Extract

Watermelon was purchased commercially and used. After removing the watermelon pulp, the watermelon was washed with water, dried in the shade, and then immersed in 80% ethanol and extracted at room temperature in an extraction container. Filtration was used to remove solids in the extract and used as a sample.

1-2: Separation of Citrulline in Watermelon Peel Extract

The following experiment was conducted to determine the content of citrulline in the watermelon peel extract extracted with 80% ethanol.

100 ppm of standard citrulline was prepared, and the solution was diluted to concentrations of 25, 50, and 100 pm, and 20 µl of each solution was applied to HPLC and detected at 254 nm. At this time, the column was YMC J 'sphere ODS-H80 (YMC J'sphere ODS-H80, 150 × 4.6 mm, S-4 ㎛, 80A), the mobile phase is CH ₃ CN and pH 6.4 sodium citrate buffer A mixed solvent in which a solution (sodium citrate buffer) was mixed at 60:40 was used, and the flow rate was performed at room temperature at 1.0 mL / min. The results regarding the standard citrulline are shown in FIGS. 1 and 2, and the equation obtained using the calibration curve is shown in Equation 1 below.

Watermelon peel extract extracted with 80% ethanol according to the present invention under the same conditions and methods as standard citrulline was also isolated by HPLC, and the citrulline content in watermelon peel extract was calculated using the obtained chromatogram. Results for watermelon peel extract according to the present invention is shown in Figure 3 and Table 1.

y = 0.0411x + 6.2151, R ² = 0.9917

RT (retention time, minutes) Peak Area (mV × sec) content(%) Citrulline 1.665 555.520 0.29

As shown in FIG. 3 and Table 1, citrulline in watermelon peel extract was detected after 1.665 minutes, and citrulline content in watermelon peel extract was found to be 0.29%.

1-3: Mixture preparation of watermelon peel extract and octacosanol

Watermelon peel extract was used as prepared in Example 1-1, octacosanol was used to purchase commercially available, and used as a sample to mix the watermelon peel extract and 1% octacosanol to 4: 6 weight ratio.

Experimental Example 1: Weight change and swimming experiment

1-1: Experiment Animal Culture and Sample Administration

Six-week-old Spargue-Dawley rats were purchased for in vivo experiments with a mixture of watermelon peel extract and octacosanol, with a temperature of 23 ± 1 ° C, 40-60% humidity, and 12 hours of contrast. It was adapted to the environment of the nursery in the cycle condition.

Control group (I) administered only distilled water, test group (II) administered 1% octacosanol at 30 mg / kg, test group (III) administered citrulline at 30 mg / kg, watermelon peel extract at 3 g / kg ( 10 rats for each group were divided into a test group (IV) administered with a mixture of 40%) and 1% octacosanol (60%), and a test group administered with a watermelon peel extract at 3 g / kg (V). Was administered orally once a day for 2 weeks. At this time, the watermelon peel extract used was prepared in Example 1-1, citrulline was used in Example 1-2 separated from the watermelon peel extract.

The weight change of each subject in each test group was measured and averaged for each test group at intervals of 4 days for two weeks from the time of adaptation. The results are shown in FIG.

As shown in FIG. 4, each As a result of measuring oral administration of samples for two weeks for each test group, the weight change of the rats showed a constant increase in all experimental groups including the control group (I), and there were no dead individuals among the test animals. All of the compositions were judged to have no side effects and toxicity.

1-2: swimming test

 In order to measure the change in endurance of each test group after two weeks of sample administration, a swimming test was conducted as follows.

A weight of 8-10% of the weight of the subjects of each test group was attached to the vessel and floated in a water bath maintained at 24 ± 1 ° C. The time from the start to the time when the test animal completely submerged in water and did not rise for more than 10 seconds was measured. The results are shown in Table 2 and FIG.

I Ⅱ Ⅲ Ⅳ Ⅴ Average (seconds) 285.1 302 371.1 360 267.9 Standard Deviation 8.08 8.6 8.16 8.4 8.52

As shown in Table 2 and Figure 5, compared to the control group (I), in the test group (III) administered citrulline, the floating time in water increased by about 30%, watermelon peel extract and octacosanol of the present invention In the test group (IV) administered with the mixture of, the increase was about 26%, and in the test group (II) administered 1% octacosanol, the increase was about 6%. ) Showed a slight reduction effect, indicating that the endurance was significantly improved in the test group (III) and the test group (IV).

Experimental Example 2: Biochemical Analysis

The following experiment was performed to measure the degree of improvement in liver function and the endurance of the rats of each test group administered orally with the sample for 2 weeks.

After the swimming test was performed on the rats in each group, serum was taken from the experimental animals and the livers were extracted.

2-1: Measurement of liver function improvement

Liver weight was measured for liver analysis to determine the degree of improvement in liver function of each test group. C), GOT (glutamate oxaloacetate transaminase), GPT (glutamate pyruvate transaminase) were measured using an assay kit. Assay kits used were total TC content of AM 202-K (Asan Pharmaceutical), TG content of AM 157S-K (Asan Pharmaceutical), HDL-C content of AM 203-K (Asan Pharmaceutical), GOT and GPT AM 101-K (Asan Pharmaceuticals) was used respectively. Each analysis result is shown to Table 3-Table 8, and FIGS. 6-10.

Average liver fresh weight of each test group subject Test group Liver Live Weight (g) I 7.54 ± 0.237 Ⅱ 7.41 ± 0.404 Ⅲ 7.82 ± 0.477 Ⅳ 7.57 ± 0.25 Ⅴ 7.41 ± 0.341

As shown in Table 3, the liver liver weight extracted after the swimming test was measured to have almost the same liver weight of all test groups as compared to the weight of the control group (I).

Average TC value in the liver of each test subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 28.93175 30.49852 23.67953 23.36795 23.5095 Standard Deviation 17.2106825 5.78085504 12.602003 14.5198673 20.5928662

As shown in Table 4 and FIG. 6, the TC values of the test group (III) and the test group (IV) were lower than those of the control group (I), and the test group (II) and the control group (II). Almost the same value.

Average TG levels in the liver of each test subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 676.6086 637.8016 625.5075 629.1842 587.6676 Standard Deviation 136.2418 115.7502 154.1881 127.8012 52.34584

As shown in Table 5 and FIG. 7, the TG levels of all the test groups decreased compared to the TG levels of the control group (I), and a significant TG reduction effect was observed in the test group (III) and the test group (IV).

Average HDL-C levels in the liver of each test subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 31 29.22449 34.33107 38.95692 28.85204 Standard Deviation 5.76533 5.44229 6.33107 6.95692 5.86508

As shown in Table 6 and FIG. 8, there was a slight increase in test group (IV) and test group (III) compared to HDL-C levels of control group (I).

Average GOT levels in the liver of each test subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 1.201898 1.172728 1.234804 1.303429 1.302361 Standard Deviation 0.096051 0.068495 0.0849319 0.038829 0.047998

As shown in Table 7 and FIG. 9, all test groups showed measurements almost similar to the GOT values of the control group (I).

Average GPT levels in liver of each test group I Ⅱ Ⅲ Ⅳ Ⅴ Average 1.227922 1.195463 1.14393 1.224522 1.14878 Standard Deviation 0.0255427 0.05166497 0.16865601 0.1493284 0.02571752

As shown in Table 8 and FIG. 10, the measurements were similar to the GPT values of the control group (I) in all test groups similar to the GOT values.

 From the above results, it can be seen that citrulline alone has an effect on improving liver function. In addition, in the case of taking into consideration the effect of improving liver function similar to that of the test group (III), even in the test group (IV), the citrulline dosage of the composition of the present invention was 3.48 mg / kg, which was only 1/10 times that of the test group (III). In the watermelon peel extract, citrulline and octacosanol showed a synergistic effect.

2-2: Measurement of muscle endurance improvement and blood sugar lowering effect

Serum TC, TG, HDL-C, GOT, GPT, lactic acid, inorganic phosphate (IP), creatine kinase, for serum analysis to determine the endurance of each test group CK) activity and glucose were measured using an assay kit. The kit used was the same as that used in Example 2-1 for TC, TG, HDL-C, GOT and GPT, lactic acid was lactate (Roch), IP was inorganic phosphorous reagents (Rayer). CK used Creatine kinase reagent, and blood glucose used Gluco-quant (Roche), respectively. Each analysis result is shown to Tables 9-17, and FIGS. 11-19.

Mean TC Value in Serum of Each Test Group Subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 76.11111 77.7 75.9 66 72.4 Standard Deviation 17.149668 14.7576722 18.6097346 5.83095189 17.4877735

As shown in Table 9 and Figure 11, it can be seen that the TC value is significantly reduced in the test group (IV) compared to the control (I).

Average TG levels in serum of each test group subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 63.14286 60.71429 58.42857 58.57143 61 Standard Deviation 15.8264396 19.1982142 15.5441001 7.87098348 12.780193

As shown in Table 10 and FIG. 12, TG levels were slightly decreased in the test group (III) and the test group (IV) compared to the control group (I).

Average HDL-C levels in serum of each test group subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 25.57143 25.125 27.44444 27.22222 24.44444 Standard Deviation 4.42933941 3.13676357 4.77260702 2.43812314 4.05517502

As shown in Table 11 and FIG. 13, HDL-C levels showed nearly similar measurements compared to Control (I) in all test groups.

Mean GOT Level in Serum of Each Test Group Subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 117.1111 108.7 115 100.32527 108.8 Standard Deviation 18.1620789 13.2669179 19.3792558 10.3252657 20.6225335

As shown in Table 12 and FIG. 14, the GOT value was the lowest in the test group (IV) compared to the control group (I).

Mean GPT Levels in Serum of Each Test Group Subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 42.85714 42.77778 40.33333 39.66667 42.33333 Standard Deviation 8.11230694 6.49572509 7.58287544 3.31662479 4.09267639

As shown in Table 13 and FIG. 15, GPT values were the lowest in the test group (IV) compared to the control group (I).

Average CK concentration in serum of each test group subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 549.1111 573.7143 659.6 668.7778 514.7778 Standard Deviation 68.15872 42.558643 65.8351477 69.2744141 65.459487

As shown in Table 14 and FIG. 16, creatine kinase concentrations increased by about 21% in test group (IV) and about 20% in test group (III) compared to control group (I).

Average Inorganic Phosphate Concentration in Serum of Each Test Group I Ⅱ Ⅲ Ⅳ Ⅴ Average 12.01111 13.5 13.03 12.94444 14.55714 Standard Deviation 2.51666667 1.75689119 3.21387616 1.51005887 0.87722506

As shown in Table 15 and FIG. 17, the values of the test group (V) slightly increased compared to the control group (I), and the measured values of the remaining (II), (III), and (IV) groups showed little change.

Average Lactic Acid Concentration in Serum of Each Test Group Subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 156.6625 130.9 137.7625 125.525 144.8 Standard Deviation 40.5837736 88.3828462 13.3383376 34.4289641 41.9288445

As shown in Table 16 and FIG. 18, the value of the test group (IV) group was about 19%, about 16% in the test group (II), about 12% in the test group (III), as compared to the control group (I). In addition, the concentration of about 7% decreased in the test group (V).

Average glucose concentration in serum of each test group subject I Ⅱ Ⅲ Ⅳ Ⅴ Average 105.7 87.4 84.5 80.33333 92 Standard Deviation 25.1663355 16.8338284 13.721435 14.39618 19.4193489

As shown in Table 17 and FIG. 19, the value of the test group (IV) was about 24%, about 20% in the test group (III), about 17% in the test group (II), and also compared with the control group (I). The concentration of about 12% was decreased in the test group (V).

From the above serum analysis results, it was confirmed that the watermelon peel extract of the present invention and the octacosanol mixture reduce serum total cholesterol content, triglyceride content, and reduce GOT and GPT to improve liver function.

In addition, the concentration of creatine kinase, which catalyzes the synthesis of ATP, and the concentration of inorganic phosphate, which is involved in the production of muscle fibers, were increased, and the level of lactic acid and blood glucose, which is a kind of fatigue substance accumulated in the muscle cytoplasm, was reduced. It became. This effect was observed even when only citrulline was administered. In the test group (IV), the citrulline dosage of the composition of the present invention was 3.48 mg / kg, which was only 1/10 times that of the test group (III). Considering similar stamina enhancement and hypoglycemic effects, citrullin and octacosanol in watermelon peel extracts showed synergistic effects.

Examples of preparations for the compositions of the present invention are illustrated below.

Formulation Example 1 Preparation of Health Food

A composition comprising a mixture of watermelon peel extract and octacosanol of the present invention was prepared as follows.

1-1: Preparation of Beverages

Instant sterilization by homogeneously mixing a mixture of subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and watermelon peel extract and octacosanol After that, it was packaged in small packaging containers such as glass bottles and plastic bottles to prepare a healthy beverage.

1-2: Preparation of Powder

       2 g of a mixture of watermelon peel extract and octacosanol of the present invention

1g lactose

The above ingredients were mixed and filled in airtight cloth to prepare a powder.

1-3: Preparation of Flour Foods

0.5 to 5.0 parts by weight of a mixture of watermelon peel extract and octacosanol of the present invention was added to flour, and bread, cake, cookies, crackers, and noodles were used to prepare health-promoting foods.

1-4: Manufacturing of Wire

Brown rice, barley, glutinous rice, and yulmu were alphad by a known method, and then dried and roasted to prepare a powder having a particle size of 60 mesh.

Black beans, black sesame seeds, and perilla were also steamed and dried by a known method, and then ground to a powder having a particle size of 60 mesh.

The mixture of watermelon peel extract and octacosanol of the present invention was concentrated under reduced pressure in a vacuum concentrator, and the dried product obtained by drying with a sprayer and a hot air dryer was pulverized with a particle size of 60 mesh to obtain a dry powder.

The dry powder of the above-mentioned grains, seeds and watermelon peel extract and octacosanol mixture was prepared in the following ratio.

Cereals (30 parts by weight brown rice, 15 parts by weight of radish, 20 parts by weight of barley),

Seeds (7 parts by weight perilla, 8 parts by weight black beans, 7 parts by weight black sesame seeds),

       Dry powder (3 parts by weight) of the mixture of watermelon peel extract and octacosanol of the present invention,

Ganoderma lucidum (0.5 parts by weight),

Foxglove (0.5 weight part)

As described above, the composition containing the mixture of watermelon peel extract and octacosanol of the present invention has an effect of improving liver function by reducing cholesterol and triglyceride content in the body, increasing the concentration of creatine kinase in the blood and increasing the concentration of lactic acid in the blood. It has a stamina enhancing effect, and also has a blood sugar enhancing effect.

Claims (5)

A composition for enhancing stamina containing a mixture of watermelon peel extract and octacosanol extracted with water, alcohol or a mixed solvent thereof. delete delete The composition of claim 1, wherein the watermelon peel extract is extracted with 80% ethanol. The composition of claim 1, wherein the mixture is mixed with a watermelon peel extract and 1% octacosanol in a weight ratio of 4: 6.

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