KR100696575B1 - Foodstuff for Relieving Stress Comprising Tortoiseshell Powder and Vitamin E Powder - Google Patents

Abstract

The present invention relates to a food comprising a composition of Zara persimmon powder and powdered vitamin E in an amount of 20: 1 to 30: 1 (w / w) and exhibiting a stress relieving effect. Since the food of the present invention may promote the recovery of the physical condition lowered by stress, it may be widely used for health promotion.

Tortoiseshell, Powdered Vitamin E, Stress Relief

Description

Foodstuff for Relieving Stress Comprising Tortoiseshell Powder and Vitamin E Powder

The present invention relates to a food having a stress relieving effect comprising Jara starch powder and powdered vitamin E. More specifically, the present invention relates to a food comprising a composition of Zabarbap powder and powdered vitamin E in a range of 20: 1 to 30: 1 (w / w) and exhibiting a stress relieving effect.

Generally, stress refers to a nonspecific biological reaction occurring in the body for various causes applied to the living body, and in the early stage, hormones in the body are abnormally secreted into the bloodstream by hormones such as adrenaline. The balance is impaired and the endocrine system is disturbed. The metabolic activity is abnormally performed by the disturbed endocrine system, and the tissues and organs in the body are gradually damaged and finally expressed in the form of a disease. The causes of such stress are also called stressors or triggers, which can be broadly classified into external and internal causes. External causes are external factors that can affect each individual's thinking and behavior, such as noise, powerful light and heat, physical environments such as confined spaces, disrespect and command, and social conflicts such as conflict with others. Examples include organizational relationships such as relationships, rules, regulations, and formalities, deaths of relatives, loss of jobs, big events in life such as promotions, and daily complications such as commuting. In addition, internal causes are the characteristics of each individual who accepts and responds to the external causes, for example, negative sleep, such as insufficient sleep, lifestyle choices such as heavy schedules, pessimistic thinking, self-abuse, or excessive analysis. , Unrealistic expectations, self-righteous possessions, exaggerated and rigid thinking.

Moderate stress is known to energize the body and mind, but excessive stress can cause emotional anxiety and conflict, and can lead to persistent dysfunction of the autonomic nervous system, causing physical dysfunction or illness. Efforts are being made to treat physical dysfunction. In particular, research is being conducted to treat physical dysfunction, which is expressed as a symptom rather than a mental damage that varies greatly from individual to individual.

For example, Korean Patent Registration No. 52907 discloses a therapeutic agent for stress disorders including new pyridyl and pyrimidyl derivatives, and Korean Patent Registration No. 132701 discloses a therapeutic agent for stress disorders comprising rhizome root extracts Korean Patent Registration No. 155976 discloses one type of choline acid selected from urosodeoxycholine acid, taurorusode oxycholine acid, chenodeoxycholine acid, dihydrocholine acid, or animal bile. An anti-stress pharmaceutical composition is disclosed, and Korean Patent Registration No. 356747 discloses a stress-relieving beverage containing fucoidan derived from seaweed extract and wakame cotyledon and its manufacturing method, and Korean Patent Registration No. 379901 describes Rose yogurt capable of treating stress gastrointestinal diseases and preparation method thereof Korean Patent Registration No. 379904 discloses chrysanthemum yogurt and a method of manufacturing the same, which can treat stress gastrointestinal diseases. However, since the above-described conventional technique is only a method of treating only some of the physical diseases shown after the accumulated stress appears to be a physical disease that can be perceived by the person, it can be various There was a disadvantage in that it could not effectively prevent or effectively cope with various diseases.

If stress is able to suppress abnormal metabolic activity before the onset of specific disease, it is predicted that the fundamental resolution of stressful disease is expected to be possible, and studies related to this have been actively conducted. No results have been reported.

Therefore, the necessity of developing a method that can fundamentally solve the stressful disease is constantly emerging.

Accordingly, the present inventors have diligently researched to develop a method for fundamentally resolving stressful diseases. As a result, when the food containing the powder of the dried spine (tortoiseshell) and the powdered vitamin E are common, By inhibiting abnormal metabolic activity, it was confirmed that the stress disease can be fundamentally solved, and completed the present invention.

After all, the main object of the present invention is to provide a food that exhibits a stress relief effect comprising the dried Jara powder and the composition of powdered vitamin E.

Foods exhibiting the stress relief effect of the present invention comprises a composition of Zara starch powder and powdered vitamin E from 20: 1 to 30: 1 (w / w): wherein the dried Zara starch powder is not particularly limited thereto. However, the dried powder powdered and dried in the shade after steaming Zarabyeol; Alternatively, it is preferable to use a hot water treatment of the jar, and to dry the hot water treated jar, and to use it in the form of small powder fired and powdered at a high temperature, and most preferably, the small powder of jar.

In order to detect abnormal metabolic activity due to stress before the onset of a specific disease caused by stress, the present inventors artificially induce stress in a range where no disease or physical damage occurs, and then variously exist in the body. As a result of detecting the activity of enzymes, unlike serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGTG), in which activity does not change only after hepatocytes are damaged by stress, lactate dehydrogenase (LDH) is used even if hepatocytes are not damaged. Since the enzyme activity was increased in proportion to the intensity of the stress, it was predicted that the level of LDH could be used as an indicator to confirm the degree of stress.

Accordingly, the present inventors target various components including goji berry extract, taurine, powdered vitamin E, nicotinic acid amide, etc., which are known to be effective in the recovery of stress fatigue, in order to develop a substance that can suppress abnormal metabolic activity caused by stress. Using the LDH activity in the body, they searched for substances that could have a stress-relieving effect.

The turtle is a reptile belonging to the turtle neck, Jaraaceae, and has been used as a tonic and aphrodisiac since ancient times, and in particular, oriental medicine has used tortoise, the spine of the turtle, as a valuable herbal medicine. For example, in the synonym of the guardian, "Seulpagi controls the abnormalities of organs, protects the body and blood, and reinforces physiological function and strength." However, tortoiseshell contains abundant protein and fat and is easily deteriorated at room temperature. However, in one case, the tortoiseshell is treated with steam, and then dried in the shade to be preserved. In order to improve the preservation by processing a short shell for a short time. For example, Korean Patent Registration No. 302054 discloses a processing method of a tortoiseshell, comprising the steps of hot water treatment and drying of the tortoiseshell, followed by baking at a high temperature in a kiln, and powdering.

The present inventors have found out that the powdered Jagabap powder can exhibit an effective stress relieving effect than goji berry extract, taurine, powdered vitamin E, and nicotinic acid amide, which are known to have excellent stress fatigue recovery effects.

On the other hand, in order to maximize the stress relief effect of the above-mentioned Jara persimmon powder, by mixing the Zara persimmon powder and the components exhibiting various stress releasing effects, it was confirmed whether or not to increase the stress relief effect, goji berry extract, taurine, Although nicotinic acid amide did not have a significant effect on the stress-relieving effect of Zara-starchy powder, it was confirmed that powdered vitamin E could increase the stress-relieving effect of Zara-starchy powder. And the mixing ratio of the powdered vitamin E was found to be 20: 1 to 30: 1 (w / w).

The composition of Jara starch powder and powdered vitamin E can be prepared in the form of food for stress relief by mixing with various food additives. In other words, Jara starch powder and powdered vitamin E include sugars (e.g., monosaccharides, disaccharides, polysaccharides, sugar alcohols, etc.), flavors (e.g., taumartin, stevia extract, saccharin, aspartame, etc.), nutrients, powdered vitamins, edible electrolytes. Mixed with food additives such as flavors, colorants, neutralizers (e.g. cheese, chocolate, etc.), pectic acid, alginic acid, organic acid, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonating agents It may be prepared in the form of food for stress relief. At this time, the form of the food is not particularly limited thereto, but may be prepared by adding in the manufacture of a variety of foods, including wire, noodles, confectionery, beverages, meat, fish, na logistics, steamed rice or rice, etc., to solve the electrical stress The addition amount of the jara powder and the powdered vitamin E composition added to the food is not particularly limited.

Since the food of the present invention may promote the recovery of the physical condition lowered by stress, it may be widely used for health promotion.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Example 1 Search for Ingredients Showing Stress Relief Effect

Example 1-1 : Search for a method capable of detecting abnormal metabolic activity due to stress

In order to detect abnormal metabolic activity caused by stress, stress can be artificially induced in a range where no disease or physical damage occurs before stress causes a specific disease, and then the activity of various enzymes present in the body is detected. The enzyme was detected and searched for an activity proportional to the intensity of the stress.

That is, Sprague Dawley rats with a body weight of 300-350 g were subjected to forced swimming once a day (five minutes per time) for two weeks to apply physical stress and restrained for six hours a day. Using a cage to stress mental, 1 day, 3 days. After 7 and 14 days, the rats of each experimental group were collected and the enzyme activity of serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), and lactate dehydrogenase (LDH) in the blood was collected. SGOT enzyme activity measurement kit (C-101, MBL, Korea), SGPT enzyme activity measurement kit (C-102, MBB) L, Korea), and LDH enzyme activity measurement kit (C-104, MBL, Korea). At this time, a control rat and a rat without a restraint cage were used.

Changes in Enzyme Activity over Time in Stressed Rats (IU / L) Elapsed time (days) Enzyme Activity of Control Group Enzyme Activity of Experimental Group SGPT SGOT LDH SGPT SGOT LDH 0 47.4 ± 4.3 71.5 ± 7.5 1589 ± 140 46.0 ± 3,5 69.1 ± 4.4 1558 ± 130 One 46.8 ± 4.7 71.7 ± 6.8 1579 ± 112 46.0 ± 3,5 70.4 ± 6.8 1675 ± 152 3 46.2 ± 3.8 72.8 ± 7.1 1603 ± 128 47.2 ± 2,3 71.5 ± 5.9 1882 ± 136 7 49.8 ± 3.2 72.3 ± 9.1 1651 ± 122 50.4 ± 2,3 93.2 ± 7.1 2475 ± 171 14 46.9 ± 4.9 70.8 ± 6.8 1635 ± 152 84.2 ± 4,1 112.8 ± 6.5 2852 ± 212

As shown in Table 1, the enzyme activity of SGPT and SGOT did not change before 7 days had elapsed, but only the enzyme activity of LDH had changed, but after 7 days all of the enzyme activities of SGPT, SGOT and LDH had been changed. It was found to increase.

Considering that the enzyme activity of SGPT and SGOT is a standard measure of liver function, the liver was not damaged for 7 days after stress, but it was found that the liver was damaged after 7 days. Enzyme activity was not suitable for detecting abnormal metabolic activity due to stress before the onset of specific disease caused by stress.

On the other hand, since LDH gradually increased the enzyme activity from the day after stress, it was confirmed that it is suitable for detecting abnormal metabolic activity due to stress before the onset of specific disease caused by stress.

Example 1-2 : Search for ingredients showing stress relief effect

Goji berry extract, powdered vitamin E, taurine, nicotinic acid amide, and Zara-stargae powder, which are known to be effective in stress fatigue recovery, were searched for components that showed stress relief effect using blood LDH levels.

Sprague Dawley rats with a body weight of 300-350 g were purchased and subjected to an acclimatization period in an animal laboratory, followed by 12 animals per treatment group by randomized complete block design in the same manner as in Example 1-1 (forced swimming, Restraint cage) caused stress fatigue. The experimental groups were divided into five groups and then forced swimming was performed once a day (5 minutes per day) for 7 days after the test was started, and at the same time, they were subjected to mental stress using a restraint cage for 6 hours a day. It was. Each experimental group of groups 1, 2, 3, 4, and 5 was administered orally once daily with goji berry extract, powdered vitamin E, taurine, nicotinic acid amide, and Jaracala powder in an amount of 10 mg / kg body weight. At this time, the Gojija extract extracts the Gojija with 70% (v / v) ethanol solution, and then using a dry dry powder, the Jarajeap powder is treated with steam and then dried in the shade and dried powdered powder Was used. On the other hand, the negative control group did not apply the electrical stress and did not administer the sample, and the positive control group applied the stress but did not administer the sample. Seven days after the initiation of the experiment, the rats were fasted for 12 hours, anesthetized with ethyl ether, and the animals were sacrificed. Blood samples for analysis were taken from the abdominal main artery. , Enzyme activity of the LDH contained in the blood sample was measured (see Table 2).

Changes in LDH Enzyme Activity According to the Types of Stress-Resisting Components in Stressed Rats (IU / L) Experimental group LDH Enzyme Activity Experimental group 1 1,953 ± 152.2 Experiment group 2 1,343 ± 135.5 Experiment group 3 1,743 ± 142.1 Experimental Group 4 1.542 ± 161.7 Experimental group 5 1.102 ± 141.4 Negative Control 2,580 ± 162.2 Positive control group 1,675 ± 104.1

As shown in Table 2, the LDH enzyme activity of each experimental group is lower than the positive control group, it was confirmed that the Jara starch powder is particularly effective in reducing the LDH enzyme activity.

The results show that Jalabap powder has better stress relief effect than other components.

Example 1-3 Search for Ingredients That Can Assist in Stress Relieving Effect

In Example 1-2, it was confirmed that Zarabap powder most effectively lowered the LDH value, and thus the stress relieving effect was the best, and to search for a component that can improve the stress relief effect of the Zarabap powder.

That is, for 7 days, forced swimming was performed once a day (5 minutes per time) to the rats of 300-350 g body weight, and physical stress was applied at the same time. (Experimental group 8-12 group). During the experimental period, group 8 had 10 mg + powdered Goji extract per kg body weight, group 9 had 10 mg + powdered vitamin E 10 mg per kg body weight, and group 10 had 10 mg + taurine 10 mg per kg body weight. The group was orally administered 10 mg / g nicotinic acid amide 10 mg per kg body weight, and the group 12 orally once a day. At this time, the Goji berry extract and Jara star powder was used in the same manner as in Example 1-2. On the other hand, the control group was stressed, but did not administer the stress fatigue recovery component. Then, in the same manner as in Example 1-2, blood LDH levels were measured and compared (see Table 3).

Changes in LDH Enzyme Activity According to the Kinds of Stress-Resisting Components in Growing Star Powder and Stress-Resisting Components in Stressed Rats (IU / L) Experimental group LDH Enzyme Activity Experimental Group 8 1,122 ± 121.7 Experimental Group 9 934 ± 131.8 Experimental group 10 1,164 ± 151.2 Experimental Group 11 1,111 ± 141.4 Experimental Group 12 1,262 ± 172.1 Control 2,180 ± 198.6

As shown in Table 3 above, all the experimental groups in which the mixed Jara powder and Goji berry extract (Experimental group 8), taurine (Experimental group 10), nicotinic acid amide (Experimental group 11) were mixed compared to the experimental group 12 administered only Jarabap powder. Although the LDH enzyme activity was shown to be lowered, the experimental group administered with a mixture of Jara powder and powdered vitamin E (Experimental Group 9) was found to exhibit an excellent effect of lowering LDH enzyme activity.

Therefore, it was confirmed that more effective stress relief is possible when the combination of Jaracara powder and powdered vitamin E is administered.

Example 2 Determination of Optimum Composition Ratio of Jara Starpump Powder and Powdered Vitamin E

As shown in Example 1-3, since the composition containing Zara starch powder and powdered vitamin E may exhibit a stress releasing effect, the composition ratio of Zara starch powder and powdered vitamin E showing an optimal stress mitigation effect was determined.

That is, for 7 days, forced swimming was performed once a day (5 minutes per time) to the rats of 300-350 g body weight, and physical stress was applied at the same time. (Experimental groups 13-22). During the experimental period, Experimental group 13 had 10 mg of a composition mixed with powdered vitamin E and powdered vitamin E per kg body weight of 0: 1 (w / w), and experimental group 14 had 1: 1 (growth of powdered vitamin E and powdered vitamin E per kg body weight). 10 mg of the composition mixed with w / w), experimental group 15, 10 mg of the composition mixed with powdered vitamin E and 5: 1 (w / w) per kg body weight, and experimental group 16, the powdered vitamin and the powdered vitamin per kg body weight, experimental group 16 10 mg of composition E mixed at 10: 1 (w / w), experimental group 17 per kg body weight, 10 mg of composition mixed with E. starch powder and powdered vitamin E at 15: 1 (w / w), experimental group 18 per kg body weight 10 mg of a composition containing 20: 1 (w / w) of jara powder and powdered vitamin E, and experimental group 19, 10 mg of a composition of 25: 1 (w / w) of jara powder and powdered vitamin E per kg body weight, Experimental group 20 had 10 mg of a composition containing 30: 1 (w / w) of Zarabap powder and powdered vitamin E per kg of body weight, and experimental group 21 contained 35: 1 (w / w) of Jarabap powder and powdered vitamin E per kg of body weight. Mixed with Composition 10 mg and Experimental group 22 was orally administered once a day 10 mg of a composition mixed with powdered vitamin E and 40: 1 (w / w) per kg body weight. At this time, Zarabap powder and powdered vitamin E were used in the same manner as in Example 1-2. On the other hand, the control group was applied to the stress, but the stress-relieving component was not used in the rat.

Then, LDH enzyme activity was measured using the method of Example 1-2 (see Table 4).

Changes in LDH Enzyme Activity According to the Compositional Ratios of Jara persimmon Powder and Powdered Vitamin E in Stressed Rats (IU / L) Experimental group Star Powder: Powdered Vitamin E (w / w) LDH Enzyme Activity Experimental Group 13 0: 1 1,323 ± 116.8 Experimental group 14 1: 1 1,213 ± 111.5 Experimental group 15 5: 1 1,173 ± 94.1 Experimental Group 16 10: 1 1,187 ± 114.9 Experimental Group 17 15: 1 1,113 ± 101.1 Experimental Group 18 20: 1 922 ± 121.4 Experimental Group 19 25: 1 933 ± 104.4 Experimental Group 20 30: 1 912 ± 111.4 Experimental group 21 35: 1 1,002 ± 96.4 Experimental Group 22 40: 1 1,032 ± 121.4 Control - 2,253 ± 193.1

As shown in Table 4, although the composition ratio of Zarabap powder and powdered vitamin E was 1: 1 to 30: 1 (w / w), the ratio of LDH enzyme activity decreased as the ratio of Zarabap powder increased. LDH enzyme activity tended to increase after 30: 1 composition ratio of torrent powder and powdered vitamin E. In particular, it was found that LDH enzymatic activity was significantly decreased when the composition ratio of Zara-starp powder and powdered vitamin E was 20: 1 to 30: 1 (w / w).

Example 3 Preparation of Food for Stress Relief Comprising a Composition of Jara Starch Powder and Powdered Vitamin E

The composition of Zarastargap powder and powdered vitamin E, determined in Example 2, at 20: 1 to 30: 1 (w / w) was mixed with a conventional food additive to prepare a food for stress relief as follows.

Example 3-1 Preparation of Noodles for Stress Relief

Jara starch powder and powdered vitamin E are mixed in a composition of 25: 1 (w / w) 20g, 700g wheat flour, 80g eggs and 200g water to prepare a dough, to prepare a cotton noodle by the usual noodle manufacturing method, Noodles having a stress relief effect were prepared.

Example 3-2 Preparation of Biscuits for Stress Relief

Jara starch powder and powdered vitamin E are mixed in a composition of 25: 1 (w / w) 20g, 700g wheat flour, 200g milk and 80g of eggs to prepare a dough, by the conventional biscuit manufacturing method, the stress relief effect Indicative biscuits were prepared.

Example 3-3 Preparation of Drinks for Stress Relief

Jara starch powder and powdered vitamin E were mixed in a composition of 25: 1 (w / w) 20g, fruit 10mg, fruit juice 100g, sodium benzoate 10mg and 900ml of water to prepare a beverage showing a stress relief effect.

Example 3-4 Preparation of Soybean Sprouts for Stress Relief

Jara starch powder and powdered vitamin E are mixed in a composition of 25: 1 (w / w) 20g, red pepper powder 150g, a small amount of salt, garlic, pepper, onion and ginger to prepare a seasoning for bean sprouts, electric 200 g of the prepared seasoning and 500 g of bean sprouts were mixed, and the bean sprouts to which the stress relieving effect was exhibited were prepared in a conventional manner.

Example 3-5 Preparation of Miso Stew for Stress Relief

Zarabap powder and powdered vitamin E are mixed in a composition of 25: 1 (w / w), 20g, miso 600g, a small amount of salt, garlic, pepper, onion and ginger, mixed in 500ml of water and cooked in the usual manner. To prepare a doenjang crab exhibiting a stress relief effect.

Example 3-6 Preparation of Multigrain Rice for Stress Relief

Jara starch powder and powdered vitamin E are mixed in a composition of 25: 1 (w / w) 20g, black soybean 50g, sorghum 50g, millet rice 50g and white rice 300g, and cooked in a conventional manner, a grain-grain rice showing the effect of stress Was prepared.

As described and demonstrated in detail above, Zarabyeolpo and powdered vitamin E comprises a composition of 20: 1 to 30: 1 (w / w), and provides a food that exhibits a stress relief effect. Since the food of the present invention may promote the recovery of the physical condition lowered by stress, it may be widely used for health promotion.

Claims (2)

Zara Stargap powder and powdered vitamin E comprising a composition of 20: 1 to 30: 1 (w / w), food for stress relief. The method of claim 1, The Jara persimmon powder is steamed Jara persimmon and dried in the shade, and dried powder or powdered dry powder or Jara persimmon is hydrothermally treated, and dried the hot water-treated Jara persimmon, which is calcined and powdered at high temperature. Characterized Food for stress relief.