KR100489267B1 - Oral care composition comprising nanoparticulated cinnamon extract - Google Patents

Abstract

The present invention relates to a composition for enhancing oral hygiene, comprising a cinnamon extract, a tocopherol derivative, coenzyme Q-10 and vitamin C as nanoparticles as an active ingredient, and a preparation method thereof. More specifically, when the total weight of the nanoparticle composition is 100% by weight, saturated lecithin 1-5.00%, ethanol 1-20.00%, capric / caprylic triglyceride 1-5.00%, cinnamon extract 1-20.00%, Oral hygiene enhancement containing nanoparticles consisting of 1-20.00% tocopherol acetate, 0.01-5.00% coenzyme Q-10, 0.01-1.00% cesylphosphate, 0.01-1.00% vitamin C, 0.1-0.50% BHT, and remaining balance of purified water It provides a composition for. In another aspect, the present invention provides a method for producing a composition for enhancing oral hygiene, comprising the steps of mixing and stirring the above-described nano-particulate components and passing through a high pressure micro homogenizer to obtain nanoparticles by cooling and defoaming. to provide.

Such an oral hygiene-promoting composition of the present invention has an excellent cavities inhibitory effect of cinnamon extract as an active ingredient, synergistic antioxidant effect with vitamin C, and the additional antioxidant effect of commonly known tocopherol derivatives and coenzyme Q-10. It is very effective in the prevention and treatment of tooth decay and periodontal disease by infiltrating into the skin and gums very quickly through the nanoparticles of people to have a continuous effect.

Description

Oral hygiene composition containing nano-particle cinnamon extract {ORAL CARE COMPOSITION COMPRISING NANOPARTICULATED CINNAMON EXTRACT}

The present invention relates to a composition for enhancing oral hygiene containing nanoparticles cinnamon extract, more specifically, containing cinnamon extract, tocopherol derivatives, coenzyme Q-10 and vitamin C in the nanoparticles as an active ingredient. It relates to a composition for promoting oral hygiene.

Oral hygiene-promoting composition is a substance for preventing and treating various oral diseases appearing in the tissues of the oral cavity, and generally contains an antimicrobial drug that suppresses or kills the propagation of many microorganisms present in the oral cavity, which is the main cause of the oral disease. . Known drugs include chlorohexidine, cetylpyridium chloride, and anti-fluoride as chemical agents, and enzymes include dextranase, which is known to have plaque deterrent effect as a fern or a coating agent. dextranase).

In view of human safety aspects, many natural antimicrobials extracted from plants are known. Korean Patent Application No. 97-73668 discloses herbal extract combinations such as bone extracts, white milk, lotus root, lotus root, and lobular leaves, showing the inhibitory effect of plaque bacteria (Japanese Patent Publication No. 55-120509), berberine extracted from yellow white Publications No. 57-5641 and 57-85319), water extracts of three combinations of five kinds of magnolia, licorice, golden and three kinds of windproof, white paper and sessin, showing the antibacterial and antigingival effects A comparative study on the inhibitory effect of plaque formation between the preparation and the herbal medicine, Kyung Hee University, 6, 7-17, 1984) has been published in the past, and the herbal extract reacts with other components of the product or Changes in color due to the change of the components of the, and also pointed out that there is a problem in reducing the efficacy of the herbal extracts, 5 ~ 500 micrometer Claims are made for a composition for promoting oral hygiene, which has been prepared as a capsule and has improved time and time stability and usability. In addition, Korean Patent Application No. 2001-6218 discloses a composition for oral hygiene enhancement containing pomegranate extract as an active ingredient, which has an excellent effect of inhibiting the generation of active oxygen and nitric oxide, thereby enhancing oral hygiene.

In addition, natural extracts known to be effective in the causative agents of caries and periodontal disease include extracts such as chamomile, myrrh, ratany, sage, and echinacea. S. de Rysky; Journal of Clinical Dentistry Vol.I, Supplement A. A22-24, 1988), Sanguinaria Extract (US Pat. No. 4,689,216), Hop Extract (JP-A-62) -138420), extracts such as Sage and Rosemary (Japanese Patent Laid-Open No. 58-134,013), essential oils extracted from mugwort (Korean Patent Application No. 2001-7858), and the like.

As such, various natural extracts have been researched and developed and applied to oral products, but in most cases, they still do not fundamentally prevent the occurrence of tooth decay and periodontal disease through poor antibacterial effects and abstract methods of use and efficacy. In addition, the problem of low bioavailability due to poor solubility of natural extracts of natural extracts remains a problem to be solved.

Accordingly, the present inventors have made a thorough research on natural products that can continuously and directly show effective effects in the treatment of oral diseases. As a result, glycoproteins and flavonoids isolated from cinnamon have a killing effect on caries, as well as UV and bacteria. The present invention has been completed based on the discovery of effectively eliminating the free radicals involved in cellular aging and necrosis that occur.

The present invention is to provide a composition for enhancing oral hygiene that can provide an effective killing effect while still showing an excellent killing effect on the cavities that are the main cause of oral diseases.

In one aspect, the present invention provides a composition for enhancing oral hygiene containing cinnamon extract, tocopherol derivatives, coenzyme Q-10 and vitamin C as nanoparticles.

In the composition for promoting oral hygiene of the present invention, the cinnamon extract is obtained by extracting by extracting cinnamon in an extraction solvent, concentrated, and purified the precipitate obtained by dissolving the concentrated cinnamon extract in water and then precipitated with ammonium sulfate. Ethyl alcohol, methyl alcohol, acetone, nucleic acid, benzene, water-ethanol and the like can be used as the extraction solvent, and ethyl alcohol is particularly preferable. Cinnamon extract may be contained in the composition of the present invention in an amount ranging from 1 to 20% by weight. In particular, the range of 5-20 weight% is preferable. When the cinnamon extract is used in less than 1% by weight, the caries bacteria killing effect is weak, and when it exceeds 20% by weight, the cinnamon extract will reduce the usability of the product.

Cinnamon is the plant name Cassia, herbal name is cinnamon (cassiae cortkex), broiler (cinnamomi cortex), cinnamon, cinnabar, scientific name is Cinnamomum cassia (Cinnamomum loureitii). In Chinese medicine, cinnamon and cinnamon are used as medicines. Cinnamon is the bark of cassia, and broiler used to refer to the bark of cinnamon. Somewhat removed, commonly used as cinnamon. Presence takes only the central part by removing the outer surface of the broiler and removing the inner skin. Gage is the thinnest, thinnest and softest of the branches of cassia. Cinnamon is a warm, nontoxic drug. The main ingredient of cinnamon is essential oil called cinnamon oil, which is cinnamic aldehyde, camphene cineol, linalool, eugenol, sugar, fat, phosphorus, iron, vitamin It contains ingredients such as A, B1, and B2. It stimulates the gastrointestinal mucosa, stimulates secretion, suppresses cramping pain in the stomach, promotes the movement of the gastrointestinal tract, and releases gas and improves absorption. In addition, cinnamon stimulates nerves, promotes blood circulation, warms the body, and has an antiseptic effect that suppresses abnormal fermentation in the intestines. Cinnamic aldehydes in cinnamon have persistent peripheral vasodilation (hypertension) and platelet aggregation inhibition, and other perspiration and clay action. Commonly used for colds, pain and pain. Cinnamon is eaten by people who are weak and lacking heat, so it is advisable not to use it if you have a fever or a lot of heat in your body. The basin of the cassia tree is particularly strong heart and promote blood circulation, so weak people with common sense. In addition, the cinnamon extract has an antimicrobial activity that suppresses the decay of food, and the effect of cinnamon extract on the skin not only prevents hair loss when the skin of the cinnamon is used on the head, but also prevents itching, heals the wound, and localizes it. It is also known to regulate congestion. Cinnamon concentrate is said to have a strong anti-inflammatory effect on the scalp, and when sprayed on the scalp from the outside, it removes wounds and inflammation of the scalp and prevents whitening to prevent white hair.

In addition, cinnamon was found to have a lethal effect on the harmful bacterium E. Coil O157: H7. Researchers at Kansas State University have demonstrated antimicrobial activity using apple juice and cinnamon heavily contaminated with the bacteria.

Cinnamon extract is known to be effective in preventing or retarding skin aging by eliminating free radicals that provide oxidative stress, which is recognized as the main mechanism of aging (Korean Patent Application No. 99-34707). Also, Korean Patent Registration No. 10-294091 discloses that cinnamon extract or green bean extract extract inhibits pCEH activity mediating the decomposition and absorption of dietary cholesterol in the small intestine, thereby preventing the absorption of dietary cholesterol in the body. It is disclosed that it is effective in preventing blood cholesterol concentration and finally preventing and treating arteriosclerosis, and in addition, it is effective in treating hyperlipidemia by reducing blood triglyceride concentration.

As such cinnamon and its extract is known for a variety of physiological activity, the inhibitory effect of the growth of caries bacteria proposed in the present invention is disclosed in the literature or is not known at all.

The composition for promoting oral hygiene of the present invention contains a tocopherol derivative known to be effective in preventing periodontal disease in addition to the cinnamon extract exhibiting the killing action of the above-mentioned caries.

Tocopherol is also called vitamin E. The substance on which vitamin E functions is called tocol, or chromane, which is based on 2-methyl-2- [trimethyltridecyl] -6-hydroxychrome only. Derivatives. The side chain of the tocol is derived from phytol, and trienol having a double bond at positions 3, 7, and 11 of the side chain has a structure similar to the tocol. Tocols and trienols are various isomers due to the position and number of methyl groups in the chroman ring. Tocopherol species include alpha, beta, gamma and delta-tocopherol. Tocopherol has antioxidant activity and prevents the oxidation of vitamin A in vivo. In addition, toothpaste is effective in preventing periodontitis. Antioxidant activity was the best in delta tocopherol with an activity ratio of alpha: beta: gamma: delta at room temperature of 1: 1.3: 1.8: 2.7. Tocopherol itself is unstable to use a lot of its derivatives, in particular there are a number of derivatives such as tocopherol acetate, tocopherol palmitate. Vitamin E is effective against severe periodontal disease. If your teeth are silver plated, the antioxidant effects of tocopherol are essential. Mercury depletes the antioxidant enzymes SOD, glutathione peroxidase and catalase in silver tissues, and animal studies have shown that tocopherols reduce silver toxicity. Therefore, tocopherol may be included in the content of 1 to 20.00% by weight as an auxiliary active ingredient that provides the periodontal disease prevention effect by the antioxidant action to the composition for promoting oral hygiene of the present invention.

Coenzyme Q-10, another active ingredient of the composition for promoting oral hygiene of the present invention, is also called ubiquinone (2,3-dimethoxy-5-methyl-6-multiprenyl-1,4-benzoquinone), It is a fat-soluble compound consisting of a quinoid nucleus having redox activity and a hydrophobic side chain comprising several monounsaturated trans-isoprenoid units. The main function of ubiquinone in vivo is to act as the redox component of the transmembrane electron transport system. Recently, much attention has been paid to the possibility that ubiquinone can act as an antioxidant and have biochemical and clinical significance. It is proposed that the substantially beneficial effect of ubiquinone can be attributed to its redox-reduction circuit and therefore can act as an antioxidant. Ubiquinone has been reported to act as a reducing agent in the organism and to protect many cell membranes from free radicals. As such, coenzyme Q-10 is responsible for energy generation and has a very effective antioxidant function as well as being widely used in periodontal disease, providing an effect of preventing periodontal disease due to antioxidant activity in the composition for promoting oral hygiene of the present invention. It serves as an auxiliary active ingredient, and may be included in an amount suitable to exhibit an effective effect, such as in a range of 0.01 to 5.00 wt%.

Vitamin C contained in the composition for promoting oral hygiene of the present invention as another active ingredient is widely known to be antioxidant, but surprisingly, when used with the cinnamon extract of the present invention, synergistically with the plant flavonoids contained in the cinnamon extract It has been found to exhibit higher synergistic antioxidant effects in the antioxidant activity than when used alone. Therefore, vitamin C can be used in the composition for promoting oral hygiene of the present invention in an amount of 0.01 to 1.00% by weight, which is suitable for showing a synergistic effect of antioxidant activity.

Specifically, in one preferred embodiment of the present invention, the present invention provides 1-5.00% saturated lecithin, 1-20.00% ethanol, capric / caprylic triglyceride 1- when the total weight of the nanoparticle composition is 100% by weight. 5.00%, Cinnamon Extract 1-20.00%, Tocopherol Acetate 1-20.00%, Coenzyme Q-10 0.01-5.00%, Cecil Phosphate 0.01-1.00%, Vitamin C 0.01-1.00%, BHT 0.1-0.50%, Remaining remaining purified water It provides a composition for enhancing oral hygiene, characterized in that containing nanoparticles made of.

As described above, the composition for promoting oral hygiene of the present invention is excellent in its antibacterial, antioxidant and free radical scavenging effects, but is highly discolored, insoluble in oil-soluble oils, and has low absorption in the body. There is still a problem that the effect cannot be sufficiently provided when blending. Therefore, in the present invention, in order to solve the problem of absorption in the body, after mixing and stirring the above-mentioned effective ingredients through a high-pressure micro homogenizer and then cooled, defoaming to make a nanoparticle size of nanometer level, for conventional oral hygiene Provided is a composition for promoting oral hygiene mixed with components contained in the composition.

In a second aspect, the present invention is a method for producing a composition for enhancing oral hygiene, as an active ingredient of the composition 1-5.00% saturated lecithin, 1-20.00% ethanol, 1-5.00% capric / caprylic triglycerides, Cinnamon Extract 1-20.00%, Tocopherol Acetate 1-20.00%, Coenzyme Q-10 0.01-5.00%, Cetylphosphate 0.01-1.00%, Vitamin C 0.01-1.00%, BHT 0.1-0.50% Mixing and stirring the content with purified water to pass through a high pressure micro homogenizer, followed by cooling and defoaming to nanoparticles, and mixing the prepared nanoparticles with components of a conventional oral hygiene composition to obtain a composition for promoting oral hygiene. It provides a method for producing an oral hygiene composition comprising a.

As a component of a conventional oral hygiene composition, for example, a toothpaste composition may contain conventional abrasives, humectants, binders, foaming agents, sweeteners, preservatives, flavors, pH adjusters, brighteners and the like.

Examples of the abrasive include calcium carbonate, calcium dihydrogen phosphate, precipitated silica, hydrous alumina, silica gel, insoluble sodium metaphosphate, zirconium silicate, and the like. These abrasives are 20 to 60% by weight, preferably 20 or more, and preferably 20 It may be used in an amount ranging from 50% by weight.

Moisturizers include glycerin, sorbitol liquid, amorphous sorbitol liquid, and the like, and these wetting agents may be used alone or in admixture of two or more, in an amount ranging from 20 to 60% by weight, preferably 20 to 50% by weight.

As the binder, carrageenan, xanthan gum, carboxymethylcellulose sodium, carboxy vinyl polymer (trade name Carbopol), sodium alginate, and the like may be used alone or in a mixture of two or more thereof, and the content of 0.1 to 3.0% by weight, preferably 0.5 to 2.0% by weight. Can be used as

As the foaming agent, anionic surfactants such as sodium lauryl sulfate and sodium lauryl sarcosinate, sorbitan fatty acid esters, polyoxyethylene hardened castor oil, polyoxyethylene polyoxypropylene-based condensed polymer materials, etc. may be mixed alone or in combination of two or more thereof. It can be used in an amount in the range of 0.5 to 5% by weight, preferably 0.5 to 2.5% by weight.

As a sweetener, sodium saccharin, aspartame, stevioside, licorice acid, etc. may be used alone or in a mixture of two or more, and used in an amount ranging from 0.05 to 0.5% by weight.

As a preservative, paraoxybenzoic acid ester, benzoic acid, sodium benzoate, methyl paraben, etc. can be used individually or in mixture of 2 or more types.

As a flavoring agent, a suitable amount of peppermint oil, spearmint oil, menthol, and carbon may be mixed, and the pH adjuster may include phosphoric acid, sodium phosphate, citric acid, sodium citrate, succinic acid, sodium succinate, tartaric acid, and sodium stannate. The pH range is suitably 5 to 8. Titanium oxide etc. can be used as a brightener.

Specifically, the method for preparing the composition for enhancing oral hygiene of the present invention can be carried out according to the formulation-specific preparation method of a composition for oral hygiene, except that the active ingredient of the present invention is added by nanoparticles. For example, in the case of a representative toothpaste composition, by heating and dissolving a fluoride and a preservative, etc. as a preparatory process, followed by stirring and dissolving a moisturizer, a sweetener and an active ingredient, and adding a powder and a viscous mixture to the mixture It can be prepared according to the manufacturing method comprising the steps of, then mixing the foam and primary defoaming, finally mixing the flavor and secondary defoaming to complete the product.

The composition for promoting oral hygiene according to the present invention prepared according to such a manufacturing method may be commercialized in the form of a variety of products for oral hygiene, for example, toothpaste, mouthwash, denture cleaner, chewing gum and the like.

Nanoparticles of the present invention, as confirmed by the frozen electron microscope, consists of two liposomes and nanoemulsions, the particle size distribution is 200 to 300nm particles. Liposomes include phosphatidyl choline (PC), phosphatidyl ethanolamine (PE), phosphatidyl serine (PS), sphingomyelins, cardiolipids, plasmalogens, Molecular groups in equilibrium with water by forming a bilayer closure membrane composed of phospholipids such as glycerol phosphatidic acid and cerebroside. Lipid components are composed of polar hydrophilic and alkyl chains by phosphoric acid and fatty acids. It is an amphiphilic substance which has in itself the nonpolar lipophilic property. Thus, liposomes, when dispersed in water, form bilayers rather than globules to capture the active ingredients of the aqueous phase. In contrast, nanoemulsions capture the active ingredient in the oil phase. The nanoparticles prepared in the present invention are 200-300 nm size particles in which poorly soluble substances such as tocopherol and coenzyme Q-10 are collected by nanoemulsion and cinnamon extracts having low bioavailability are collected by liposomes. Similarly, bioactive substances can be selectively absorbed into the mucous membranes of the skin and mouth, prevention of tooth decay, and the gums to increase the bioavailability of the active ingredients, thereby increasing the prevention and treatment effect of oral diseases.

Hereinafter, specific embodiments of the present invention will be described in more detail with reference to Examples. The present examples merely illustrate preferred embodiments of the present invention and do not limit the scope of the present invention.

Example

Example 1

Preparation of Cinnamon Extract

5 kg of cinnamon was soaked in 25 liters of 100% ethanol for 3 weeks. The extract was first filtered using filter paper, placed in a condenser and concentrated until ethanol completely evaporated. The concentrated cinnamon extract was dissolved in water, the extract dissolved in water was centrifuged (15,000 rpm, 30 minutes), the upper layer was taken only, and 80% ammonium sulfate was added, and the protein was allowed to stand at 4 ° C. overnight to precipitate. The supernatant was recovered and centrifuged at 15,000 rpm for 30 minutes at 4 ° C. to precipitate the protein. The supernatant was discarded, only pellets were taken, dissolved in water, and placed in a dialysis membrane for dialysis. Dialysis protein was lyophilized. The lyophilized protein was passed through a column (Sepadex G150) and then purified protein fractions were collected and used as cinnamon extract of the present invention.

Example 2

Preparation of Nanoparticles Containing Cinnamon Extract

With the cinnamon extract obtained as shown in Example 1, the ingredients shown in Table 1 were mixed in an amount, sufficiently stirred, passed through a high pressure micro homogenizer, and then cooled and defoamed.

ingredient Content (% by weight) Saturated Lecithin 1 to 5.00 ethanol 1 to 20.00 Capric / Caprylic Triglycerides 1 to 5.00 Cinnamon Extract 1 to 20.00 Tocopherol acetate 1 to 20.00 Coenzyme Q-10 0.01 to 5.00 Sequential phosphate 0.01 to 1.00 Vitamin c 0.01 to 1.00 BHT 0.1 to 0.50 Purified water Content to fill 100% by weight

As a result, the distribution of the prepared particles was measured using a Zeta Potential Analyzer (Malvern, Zetasizer 1000HS), and it was confirmed that the particle size was 200-300 nm (FIG. 1).

In addition, as a result of photographing by using a Photo Graphing Freeze-Fracture Scanning Electron Microscopy to observe the particle morphology, as shown in FIG. Layer liposomes could be identified. In other words, it was found that the nanoparticles are composed of nanoemulsions and liposomes.

Example 3

Inhibition of Caries Bacteria from Cinnamon Extract

In order to investigate the inhibitory effect of the caries bacteria of cinnamon extract prepared in Example 1, the experiment was carried out as follows.

1. Streptococcus Mutans, a representative strain of caries, was selected and spread evenly on solid medium.

2. Meanwhile, ethanol and cinnamon extracts (0.1, 1.0, 5, 10, 20 ul) were dropped onto a circular paper disk and dried on a clean bench.

3. When the disc dries, the disc was placed on a solid medium on which bacteria were smeared and incubated overnight at 37 ° C. incubator, and then the killing effect of the caries was analyzed.

4. At the same time, the control panel was also incubated with a plate containing a disk in which the representative antimicrobial agents Ampicillin (Amp) and Streptomycin (Stm) were dropped to the content (μg) of the numbers shown in FIGS. 3A and 3B, respectively. Incubated.

The killing effect of the cavities that can be seen by the ring formation around the disc is a cinnamon extract (Cin) of Figure 3c where a larger ring is observed compared to the ring on the ampicillin or streptomycin administration medium as shown in FIGS. 3A and 3B. Through the administration medium, the cinnamon extract was found to have a high antibacterial effect against caries.

Example 4

Antioxidant Effect of Cinnamon Extract

In order to observe the antioxidant effect and free radical scavenging effect of cinnamon extract, O ₂ and H ₂ O ₂ inhibitory activities were measured using NBT (Neo Tetrazolium Blue) method.

a) substrate reaction solution

Triton: 1.6ML

EDTA: 2.9 Mg

-NBT: 24Mg

Hypoxanthine: 5.4 Mg

Phosphate buffer (pH 7.8): 100ML

b) enzymes

Xanthine oxidase: 12 uL

Phosphate buffer (pH 7.8): 10ML

Substrate reaction solution 0.5ML, cinnamon extract 100uL, vitamin 0.01mg and enzyme 0.5ML in the UV spectrometer was added to the incubator for 30 minutes at 37 ℃ was measured by a spectrometer at 470nm. Only the buffer solution was measured as a control group (BLANK), and the comparative group was analyzed using vitamin C alone and cinnamon extract alone and BHT (Butyrated hydroxytoluene), a representative antioxidant compound. Antioxidant activity of each component was expressed by log ΔA470 / min.

As a result, the antioxidant effect was observed to be the best when using a mixture of cinnamon extract and vitamin C (Fig. 4), synergistic effect when used together with the vegetable flavonoids and vitamin C of cinnamon extract alone ( Synergy effect). In Figure 4, A is vitamin C alone, B is cinnamon extract alone, C is the antioxidant activity of the cinnamon extract and vitamin C mixture, BLANK is a control to measure the phosphate buffer. BHT shows the antioxidant activity of butyric acid hydroxytoluene, a representative antioxidant compound.

Example 5

Free Radical Scavenging Effect of Cinnamon Extracts

The free radical scavenging effect is the dimethyl extract of cinnamon extract of the present invention in 2 ml of 60 μM 1,1-diphenyl-2-picryl hydrazyl (1,1-diphenyl-2-picryl hydrazyl: DPPH) ethanol solution. After 2 ml of sulfoside (DMSO) solution was added and reacted at room temperature for 30 parts, the absorbance was measured at 520 nm to determine the free radical scavenging effect (%).

The free radical scavenging effect was found to increase as the content of cinnamon extract (CCM) increased through FIG.

As described above, by using the cinnamon extract having an excellent caries bacterium inhibitory effect, antioxidant effect and free radical scavenging effect, various kinds of toothpaste compositions can be prepared as follows.

Preparation Example 1 Nanoparticle-Containing Toothpaste Composition (1)

The nanoparticle-containing toothpaste composition of the present invention was prepared as shown below using the components shown in Table 2 below.

ingredient Content (% by weight) a) purified water Volume filling total 100% by weight    Monofluorosodium phosphate 0.80    Sodium benzoate 0.25    Polysorbitan 80 0.30 b) sorbitol 30.00    Propylene glycol 1.00    Sodium saccharin 0.50    Nanoparticles 1.00 to 5.00 c) calcium carbonate 43.00    Silica 3.00    Carboxymethylcellulose 2.00 d) sodium lauryl sulfate 2.00 e) spices A certain amount

a) is a preparation process, each component was added to the 70-75 ℃ manufacturing unit and stirred for 20 minutes to completely dissolve the powder and then cooled to room temperature. b) is a dissolution step of the moisturizer, the sweetener and the active ingredient, and was added to a 25-35 ° C. manufacturing part, followed by stirring for 15 minutes to dissolve. c) is a step of mixing powdered and viscous agent by mixing carboxy methylcellulose with calcium carbonate powder at 25-35 ° C. d) was first defoaming for 40 minutes at 25-35 ° C. as foam mixing and first defoaming. e) was prepared after degassing at 25-35 ° C. as a perfume mixing process.

Preparation Example 2 Nanoparticle-Containing Toothpaste Composition (2)

To the nanoparticle-containing toothpaste composition of the present invention was prepared according to the method shown in Preparation Example 1 using the components shown in Table 3.

ingredient Content (% by weight) a) purified water Volume filling total 100% by weight    Dicalcium Phosphate Dihydrate 48.00 b) glycerin 10.00    Sorbitol 70% solution 15.00    Methylparaben 0.10    Sodium saccharin 0.25    Nanoparticles 1.00 to 5.00 c) cellulose 1.00 d) sodium lauryl sulfate 1.50 e) peppermint oil 0.50

Preparation Example 3 Nanoparticle-Containing Toothpaste Composition (Clear Gel Toothpaste)

According to the method shown in Preparation Example 1 using the components shown in Table 4 below to prepare a cleargel type nanoparticle-containing toothpaste composition.

ingredient Content (% by weight) a) purified water Volume filling total 100% by weight    Sodium benzoate 0.50    Sodium fluoride 0.24 b) glycerin (99.5%) 10.00    Sorbitol 70% solution 50.81    Sodium saccharin 0.20    Nanoparticles 1.00 to 5.00 c) silica 22.00    PEG32 3.00 d) sodium lauryl sulfate 1.20 e) peppermint oil 1.00

Preparation Example 4 Nanoparticle-Containing Toothpaste Composition (Baked Salt Toothpaste)

Baked salt-type nanoparticle-containing toothpaste composition was prepared according to the method shown in Preparation Example 1 using the ingredients shown in Table 5 below.

ingredient Content (% by weight) a) purified water Volume filling total 100% by weight    Sodium fluoride 0.25    Titanium dioxide 0.50 b) sodium bicarbonate 20.00    Sodium saccharin 0.30    glycerin 38.00    Nanoparticles 1.00 to 5.00 c) PEG32 3.00    Silica 20.00    Cellulose gum 0.70 d) sodium lauryl sulfate 1.50 e) spices 0.75

Preparation Example 5 Nanoparticle-Containing Toothpaste Composition (Whitening Toothpaste)

Toothpaste composition containing a baked salt type nanoparticles was prepared according to the method shown in Preparation Example 1 using the components shown in Table 6 below.

ingredient Content (% by weight) a) purified water Volume filling total 100% by weight    Sodium fluoride 0.25    Titanium dioxide 0.50 b) sorbitol (70%) 24.01    Sodium saccharin 0.20    glycerin 11.60    Nanoparticles 1.00 to 5.00 c) silica 35.00    Cellulose gum 0.60 d) sodium lauryl sulfate 1.50 e) spices 1.00

As described above, the composition for enhancing oral hygiene of the present invention has an excellent inhibitory effect on caries of the cinnamon extract as an active ingredient, a synergistic antioxidant effect with vitamin C, and the additional antioxidant effects of tocopherol derivatives and coenzyme Q-10 which are widely known. In addition, the nanoparticles of these active ingredients are manufactured in a size similar to the intercellular lipid gap, and the bioactive substance is selectively absorbed into the mucous membranes and gums of the skin and mouth to enhance the bioavailability of the active ingredients, such as caries and periodontal disease. It can enhance the preventive and therapeutic effects of oral diseases.

1 is a graph showing the size distribution analyzed by Zeta Potential Analyzer (Malvern, Zetasizer 1000HS) of the nanoparticles prepared by the present invention.

Figure 2 is a micrograph of the nanoparticles taken by using a Free Graphing Freeze-Fracture Scanning Electron Microscopy.

Figure 3a is a culture photograph showing the caries bacteria inhibitory effect of ampicillin (Amp) as a comparative group.

Figure 3b is a culture photograph showing the caries bacteria inhibitory effect of streptomycin (Stm) as a comparison group.

Figure 3c is a culture photograph showing the caries bacteria inhibitory effect of cinnamon extract (Cin) of the present invention.

Figure 4 is a spectroscopic graph measuring the absorbance at 470 nm showing the synergistic effect of vegetable flavonoids and vitamin C in cinnamon extracts on antioxidant activity.

Figure 5 is a spectroscopic graph measuring the absorbance at 520nm showing the free radical scavenging effect (FRS%) for each concentration of cinnamon extract.

Claims (6)

Oral hygiene composition comprising the cinnamon extract, tocopherol derivatives, coenzyme Q-10 and vitamin C in the nanoparticles as an active ingredient. The oral hygiene composition according to claim 1, wherein the cinnamon extract is contained in a range of 1 to 20% by weight. The method according to claim 1, wherein the total weight of the nanoparticle composition is 100% by weight, saturated lecithin 1-5.00%, ethanol 1-20.00%, capric / caprylic triglyceride 1-5.00%, cinnamon extract 1-20.00% , Tocopherol acetate 1-20.00%, coenzyme Q-10 0.01-5.00%, sesyl phosphate 0.01-1.00%, vitamin C 0.01-1.00%, BHT 0.1-0.50%, the remaining amount of nanoparticles consisting of purified water Oral hygiene composition for improving. The composition for oral hygiene according to claim 1, wherein the nanoparticles are composed of liposomes and nanoemulsions and have a particle size in the range of 200 to 300 nm. The oral hygiene composition according to claim 1, wherein the composition is used in the form of a toothpaste composition. In the method for preparing a composition for enhancing oral hygiene, as an active ingredient of the composition, saturated lecithin 1-5.00%, ethanol 1-20.00%, capric / caprylic triglyceride 1-5.00%, cinnamon extract 1-20.00%, tocopherol High pressure by mixing and stirring 1-20.00% of acetate, 0.01-5.00% of coenzyme Q-10, 0.01-1.00% of cesylphosphate, 0.01-1.00% of vitamin C, and 0.1-0.50% of BHT with the content of purified water to make a total of 100% by weight Passing through a micro homogenizer and cooling and defoaming to produce nanoparticles, mixing and dissolving the nanoparticles with abrasives, moisturizers, sweeteners and viscous agents, mixing and then defoaming the mixture and foaming agent, and flavoring Method of producing a composition for enhancing oral hygiene, characterized in that it comprises a second step of defoaming after addition.