JPWO2020167656A5 - - Google Patents
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- Publication number
- JPWO2020167656A5 JPWO2020167656A5 JP2021546249A JP2021546249A JPWO2020167656A5 JP WO2020167656 A5 JPWO2020167656 A5 JP WO2020167656A5 JP 2021546249 A JP2021546249 A JP 2021546249A JP 2021546249 A JP2021546249 A JP 2021546249A JP WO2020167656 A5 JPWO2020167656 A5 JP WO2020167656A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- primer
- acid molecule
- beads
- bead
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical class 0.000 claims description 139
- 108020004707 nucleic acids Proteins 0.000 claims description 136
- 102000039446 nucleic acids Human genes 0.000 claims description 136
- 238000000034 method Methods 0.000 claims description 108
- 239000011324 bead Substances 0.000 claims description 88
- 230000003321 amplification Effects 0.000 claims description 68
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 68
- 238000005192 partition Methods 0.000 claims description 56
- 238000006243 chemical reaction Methods 0.000 claims description 26
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 230000037452 priming Effects 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 4
- 238000012163 sequencing technique Methods 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 230000000379 polymerizing effect Effects 0.000 claims description 2
- 108091093088 Amplicon Proteins 0.000 claims 3
- 239000011541 reaction mixture Substances 0.000 description 26
- 239000000969 carrier Substances 0.000 description 21
- 239000000203 mixture Substances 0.000 description 17
- 230000000295 complement effect Effects 0.000 description 12
- 239000004606 Fillers/Extenders Substances 0.000 description 7
- 239000000839 emulsion Substances 0.000 description 7
- 238000003752 polymerase chain reaction Methods 0.000 description 6
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 108010090804 Streptavidin Proteins 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962804082P | 2019-02-11 | 2019-02-11 | |
| US62/804,082 | 2019-02-11 | ||
| US201962890240P | 2019-08-22 | 2019-08-22 | |
| US62/890,240 | 2019-08-22 | ||
| US201962916683P | 2019-10-17 | 2019-10-17 | |
| US62/916,683 | 2019-10-17 | ||
| PCT/US2020/017491 WO2020167656A1 (en) | 2019-02-11 | 2020-02-10 | Methods for nucleic acid analysis |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2022519324A JP2022519324A (ja) | 2022-03-22 |
| JPWO2020167656A5 true JPWO2020167656A5 (enExample) | 2023-02-15 |
| JP7528103B2 JP7528103B2 (ja) | 2024-08-05 |
Family
ID=72045127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021546249A Active JP7528103B2 (ja) | 2019-02-11 | 2020-02-10 | 核酸分析方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US12319959B2 (enExample) |
| EP (1) | EP3924515A4 (enExample) |
| JP (1) | JP7528103B2 (enExample) |
| KR (1) | KR20210138017A (enExample) |
| CN (1) | CN113728113A (enExample) |
| AU (1) | AU2020222888B2 (enExample) |
| CA (1) | CA3129315A1 (enExample) |
| MX (1) | MX2021009482A (enExample) |
| SG (1) | SG11202108563QA (enExample) |
| WO (1) | WO2020167656A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020167656A1 (en) | 2019-02-11 | 2020-08-20 | Ultima Genomics, Inc. | Methods for nucleic acid analysis |
| CN116261593A (zh) * | 2020-08-21 | 2023-06-13 | 阿尔缇玛基因组学公司 | 用于表面扩增的组合物及其用途 |
| CN118176305A (zh) | 2021-08-20 | 2024-06-11 | 阿尔缇玛基因组学公司 | 用于测序的样品制备的系统和方法 |
| WO2023081883A2 (en) * | 2021-11-08 | 2023-05-11 | Ultima Genomics, Inc. | Methylation sequencing methods and compositions |
| WO2025072163A1 (en) * | 2023-09-25 | 2025-04-03 | Ultima Genomics, Inc. | Systems and methods for processing analytes |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9388459B2 (en) | 2002-06-17 | 2016-07-12 | Affymetrix, Inc. | Methods for genotyping |
| AU2004209001B2 (en) * | 2003-01-29 | 2007-10-11 | 454 Life Sciences Corporation | Bead emulsion nucleic acid amplification |
| JP5183063B2 (ja) * | 2003-07-05 | 2013-04-17 | ザ ジョンズ ホプキンス ユニバーシティ | 遺伝的変異の検出および列挙のための方法ならびに組成物 |
| CN1950519A (zh) | 2004-02-27 | 2007-04-18 | 哈佛大学的校长及成员们 | 聚合酶群落荧光原位测序珠子 |
| US20090203531A1 (en) | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
| EP2291533B2 (en) | 2008-07-02 | 2020-09-30 | Illumina Cambridge Limited | Using populations of beads for the fabrication of arrays on surfaces |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| US20120156728A1 (en) | 2010-12-17 | 2012-06-21 | Life Technologies Corporation | Clonal amplification of nucleic acid on solid surface with template walking |
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| WO2012047889A2 (en) | 2010-10-04 | 2012-04-12 | Genapsys Inc. | Systems and methods for automated reusable parallel biological reactions |
| WO2013023176A2 (en) | 2011-08-10 | 2013-02-14 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
| KR20150011359A (ko) | 2012-04-19 | 2015-01-30 | 라이프 테크놀로지스 코포레이션 | 디지털 pcr을 수행하는 방법 |
| WO2013158313A1 (en) | 2012-04-19 | 2013-10-24 | Life Technologies Corporation | Nucleic acid amplification |
| EP3461910B1 (en) | 2012-04-19 | 2020-08-26 | Life Technologies Corporation | Nucleic acid amplification |
| US8895249B2 (en) * | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| KR102090851B1 (ko) | 2012-08-14 | 2020-03-19 | 10엑스 제노믹스, 인크. | 마이크로캡슐 조성물 및 방법 |
| WO2014151961A1 (en) | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | Matrix arrays and methods for making same |
| WO2014144822A2 (en) * | 2013-03-15 | 2014-09-18 | Immumetrix, Inc. | Methods and compositions for tagging and analyzing samples |
| US10160995B2 (en) | 2013-05-13 | 2018-12-25 | Qiagen Waltham, Inc. | Analyte enrichment methods and compositions |
| CA2915499A1 (en) | 2013-06-27 | 2014-12-31 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| KR102596508B1 (ko) | 2014-04-10 | 2023-10-30 | 10엑스 제노믹스, 인크. | 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용 |
| EP3155127B1 (en) | 2014-06-13 | 2020-07-22 | Life Technologies Corporation | Multiplex nucleic acid amplification |
| GB201414745D0 (en) | 2014-08-19 | 2014-10-01 | Articzymes As | Exonucleases |
| WO2016187466A1 (en) * | 2015-05-20 | 2016-11-24 | Invenra, Inc. | Methods, apparatus and compositions for expression and high throughput screening |
| EP3365445B1 (en) | 2015-10-19 | 2023-05-31 | Dovetail Genomics, LLC | Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection |
| JP7064439B2 (ja) * | 2015-11-04 | 2022-05-10 | アトレカ インコーポレイテッド | 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット |
| US10730030B2 (en) * | 2016-01-08 | 2020-08-04 | Bio-Rad Laboratories, Inc. | Multiple beads per droplet resolution |
| CN109312396A (zh) | 2016-04-07 | 2019-02-05 | 伊鲁米那股份有限公司 | 用于构建标准化核酸文库的方法和系统 |
| WO2018017884A1 (en) | 2016-07-20 | 2018-01-25 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
| WO2018064297A1 (en) | 2016-09-29 | 2018-04-05 | Ultima Genomics, Inc. | Systems and methods for nucleic acid sequencing |
| US10011872B1 (en) * | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| JP2018183097A (ja) | 2017-04-26 | 2018-11-22 | 株式会社エンプラス | 細胞由来の解析用液滴の単離方法、および細胞の解析方法 |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| WO2019079653A1 (en) | 2017-10-19 | 2019-04-25 | Ultima Genomics, Inc. | METHODS OF TREATING APPARATUSED END SEQUENCES |
| CN111566223B (zh) | 2017-11-07 | 2024-04-12 | 生命技术公司 | 用于操纵核酸的方法和组合物 |
| US12226745B2 (en) | 2017-11-07 | 2025-02-18 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| CN112005115A (zh) * | 2018-02-12 | 2020-11-27 | 10X基因组学有限公司 | 表征来自单个细胞或细胞群体的多种分析物的方法 |
| WO2020167656A1 (en) | 2019-02-11 | 2020-08-20 | Ultima Genomics, Inc. | Methods for nucleic acid analysis |
| WO2020172197A1 (en) | 2019-02-19 | 2020-08-27 | Ultima Genomics, Inc. | Linkers and methods for optical detection and sequencing |
| CN116261593A (zh) | 2020-08-21 | 2023-06-13 | 阿尔缇玛基因组学公司 | 用于表面扩增的组合物及其用途 |
| EP4320274A4 (en) | 2021-04-09 | 2025-03-05 | Ultima Genomics, Inc. | SYSTEMS AND METHODS FOR SPATIAL SCREENING OF ANALYTES |
-
2020
- 2020-02-10 WO PCT/US2020/017491 patent/WO2020167656A1/en not_active Ceased
- 2020-02-10 CN CN202080027939.0A patent/CN113728113A/zh active Pending
- 2020-02-10 CA CA3129315A patent/CA3129315A1/en active Pending
- 2020-02-10 MX MX2021009482A patent/MX2021009482A/es unknown
- 2020-02-10 JP JP2021546249A patent/JP7528103B2/ja active Active
- 2020-02-10 KR KR1020217028674A patent/KR20210138017A/ko not_active Ceased
- 2020-02-10 EP EP20755711.7A patent/EP3924515A4/en active Pending
- 2020-02-10 AU AU2020222888A patent/AU2020222888B2/en active Active
- 2020-02-10 SG SG11202108563QA patent/SG11202108563QA/en unknown
-
2021
- 2021-08-05 US US17/394,692 patent/US12319959B2/en active Active
-
2025
- 2025-07-28 US US19/282,712 patent/US20260078438A1/en active Pending
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