JP2019537439A5 - - Google Patents
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- JP2019537439A5 JP2019537439A5 JP2019522907A JP2019522907A JP2019537439A5 JP 2019537439 A5 JP2019537439 A5 JP 2019537439A5 JP 2019522907 A JP2019522907 A JP 2019522907A JP 2019522907 A JP2019522907 A JP 2019522907A JP 2019537439 A5 JP2019537439 A5 JP 2019537439A5
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- Prior art keywords
- nucleic acid
- primer
- adapter
- double
- item
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108020004707 nucleic acids Proteins 0.000 claims description 128
- 150000007523 nucleic acids Chemical class 0.000 claims description 128
- 102000039446 nucleic acids Human genes 0.000 claims description 128
- 238000000034 method Methods 0.000 claims description 103
- 125000003729 nucleotide group Chemical group 0.000 claims description 55
- 239000002773 nucleotide Substances 0.000 claims description 52
- 238000003786 synthesis reaction Methods 0.000 claims description 31
- 230000000295 complement effect Effects 0.000 claims description 27
- 238000003752 polymerase chain reaction Methods 0.000 claims description 13
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical group N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 12
- 230000003321 amplification Effects 0.000 claims description 11
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 11
- 239000000758 substrate Substances 0.000 claims description 11
- 201000011510 cancer Diseases 0.000 claims description 10
- 230000005298 paramagnetic effect Effects 0.000 claims description 9
- 102000003960 Ligases Human genes 0.000 claims description 8
- 108090000364 Ligases Proteins 0.000 claims description 8
- 206010028980 Neoplasm Diseases 0.000 claims description 8
- 229960002685 biotin Drugs 0.000 claims description 8
- 239000011616 biotin Substances 0.000 claims description 8
- 230000036210 malignancy Effects 0.000 claims description 8
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 7
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 7
- 238000009396 hybridization Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- 238000004458 analytical method Methods 0.000 claims description 6
- 235000020958 biotin Nutrition 0.000 claims description 6
- 230000004048 modification Effects 0.000 claims description 6
- 238000012986 modification Methods 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 108091008875 B cell receptors Proteins 0.000 claims description 5
- 108091008874 T cell receptors Proteins 0.000 claims description 5
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 claims description 5
- 239000012634 fragment Substances 0.000 claims description 5
- 108010090804 Streptavidin Proteins 0.000 claims description 4
- 210000000265 leukocyte Anatomy 0.000 claims description 4
- 238000012163 sequencing technique Methods 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- AUTOLBMXDDTRRT-JGVFFNPUSA-N (4R,5S)-dethiobiotin Chemical compound C[C@@H]1NC(=O)N[C@@H]1CCCCCC(O)=O AUTOLBMXDDTRRT-JGVFFNPUSA-N 0.000 claims description 2
- HRFIATJPGFXBNN-UFLZEWODSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid;2-[2-(2-hydroxyethoxy)ethoxy]ethanol Chemical compound OCCOCCOCCO.N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 HRFIATJPGFXBNN-UFLZEWODSA-N 0.000 claims description 2
- BUVSBIKCBLHNCG-UFLZEWODSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoic acid;azide Chemical compound [N-]=[N+]=[N-].N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 BUVSBIKCBLHNCG-UFLZEWODSA-N 0.000 claims description 2
- 208000010839 B-cell chronic lymphocytic leukemia Diseases 0.000 claims description 2
- 241000251556 Chordata Species 0.000 claims description 2
- 108010047041 Complementarity Determining Regions Proteins 0.000 claims description 2
- 108700005091 Immunoglobulin Genes Proteins 0.000 claims description 2
- 208000031422 Lymphocytic Chronic B-Cell Leukemia Diseases 0.000 claims description 2
- 206010025323 Lymphomas Diseases 0.000 claims description 2
- 208000034578 Multiple myelomas Diseases 0.000 claims description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 2
- 239000011324 bead Substances 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 208000032852 chronic lymphocytic leukemia Diseases 0.000 claims description 2
- 230000028993 immune response Effects 0.000 claims description 2
- 108020004999 messenger RNA Proteins 0.000 claims description 2
- 208000024893 Acute lymphoblastic leukemia Diseases 0.000 claims 1
- 208000014697 Acute lymphocytic leukaemia Diseases 0.000 claims 1
- 208000006664 Precursor Cell Lymphoblastic Leukemia-Lymphoma Diseases 0.000 claims 1
- 238000000137 annealing Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 230000003100 immobilizing effect Effects 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 230000008439 repair process Effects 0.000 description 3
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 238000012412 chemical coupling Methods 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical class O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical class C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 108091008915 immune receptors Proteins 0.000 description 1
- 102000027596 immune receptors Human genes 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000000865 phosphorylative effect Effects 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022166273A JP7483824B2 (ja) | 2016-11-02 | 2022-10-17 | 免疫レパートリーシーケンシングのための核酸サンプル調製の方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662416677P | 2016-11-02 | 2016-11-02 | |
| US62/416,677 | 2016-11-02 | ||
| PCT/US2017/059804 WO2018085599A2 (en) | 2016-11-02 | 2017-11-02 | Methods of nucleic acid sample preparation for immune repertoire sequencing |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022166273A Division JP7483824B2 (ja) | 2016-11-02 | 2022-10-17 | 免疫レパートリーシーケンシングのための核酸サンプル調製の方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019537439A JP2019537439A (ja) | 2019-12-26 |
| JP2019537439A5 true JP2019537439A5 (enExample) | 2020-12-17 |
| JP7161991B2 JP7161991B2 (ja) | 2022-10-27 |
Family
ID=62077010
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019522907A Active JP7161991B2 (ja) | 2016-11-02 | 2017-11-02 | 免疫レパートリーシーケンシングのための核酸サンプル調製の方法 |
| JP2022166273A Active JP7483824B2 (ja) | 2016-11-02 | 2022-10-17 | 免疫レパートリーシーケンシングのための核酸サンプル調製の方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022166273A Active JP7483824B2 (ja) | 2016-11-02 | 2022-10-17 | 免疫レパートリーシーケンシングのための核酸サンプル調製の方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10947582B2 (enExample) |
| EP (2) | EP4198140B1 (enExample) |
| JP (2) | JP7161991B2 (enExample) |
| CN (1) | CN110191959B (enExample) |
| AU (2) | AU2017355460B2 (enExample) |
| CA (1) | CA3042391A1 (enExample) |
| ES (1) | ES2940620T3 (enExample) |
| WO (1) | WO2018085599A2 (enExample) |
Families Citing this family (61)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP4026912A1 (en) | 2012-05-10 | 2022-07-13 | The General Hospital Corporation | Methods for determining a nucleotide sequence |
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| US12630864B2 (en) * | 2016-09-15 | 2026-05-19 | Laboratory Corporation Of America Holdings | Methods of nucleic acid sample preparation |
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| US12188014B1 (en) * | 2018-07-25 | 2025-01-07 | 10X Genomics, Inc. | Compositions and methods for nucleic acid processing using blocking agents |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| EP3847280A1 (en) | 2018-09-07 | 2021-07-14 | Sequenom, Inc. | Methods, and systems to detect transplant rejection |
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| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| JP7441243B2 (ja) * | 2019-05-14 | 2024-02-29 | エフ． ホフマン－ラ ロシュ アーゲー | 試料分析のための装置および方法 |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| CN111139286B (zh) * | 2019-11-07 | 2023-11-21 | 南方医科大学南方医院 | 一种bcr高通量测序文库的构建方法 |
| EP4025711A2 (en) | 2019-11-08 | 2022-07-13 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
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| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| CN121022991A (zh) | 2019-12-23 | 2025-11-28 | 10X基因组学有限公司 | 使用rna模板化连接进行空间分析的方法 |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| US12129516B2 (en) | 2020-02-07 | 2024-10-29 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| CN116157533A (zh) | 2020-02-21 | 2023-05-23 | 10X基因组学有限公司 | 使用杂交方法捕获遗传靶标 |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| EP4139485B1 (en) | 2020-04-22 | 2023-09-06 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
| EP4153775B1 (en) | 2020-05-22 | 2024-07-24 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| AU2021275906A1 (en) | 2020-05-22 | 2022-12-22 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
| EP4025692A2 (en) | 2020-06-02 | 2022-07-13 | 10X Genomics, Inc. | Nucleic acid library methods |
| EP4600376A3 (en) | 2020-06-02 | 2025-10-22 | 10X Genomics, Inc. | Spatial transcriptomics for antigen-receptors |
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| EP4446430A3 (en) | 2020-06-10 | 2024-12-18 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
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-
2017
- 2017-11-02 CA CA3042391A patent/CA3042391A1/en active Pending
- 2017-11-02 WO PCT/US2017/059804 patent/WO2018085599A2/en not_active Ceased
- 2017-11-02 EP EP22209186.0A patent/EP4198140B1/en active Active
- 2017-11-02 EP EP17868046.8A patent/EP3535405B1/en active Active
- 2017-11-02 ES ES17868046T patent/ES2940620T3/es active Active
- 2017-11-02 AU AU2017355460A patent/AU2017355460B2/en active Active
- 2017-11-02 JP JP2019522907A patent/JP7161991B2/ja active Active
- 2017-11-02 CN CN201780080046.0A patent/CN110191959B/zh active Active
- 2017-11-02 US US15/802,408 patent/US10947582B2/en active Active
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2021
- 2021-02-17 US US17/177,740 patent/US20210180118A1/en not_active Abandoned
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2022
- 2022-10-17 JP JP2022166273A patent/JP7483824B2/ja active Active
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2023
- 2023-03-06 AU AU2023201370A patent/AU2023201370B2/en active Active
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