AU2020222888B2 - Methods for nucleic acid analysis - Google Patents
Methods for nucleic acid analysis Download PDFInfo
- Publication number
- AU2020222888B2 AU2020222888B2 AU2020222888A AU2020222888A AU2020222888B2 AU 2020222888 B2 AU2020222888 B2 AU 2020222888B2 AU 2020222888 A AU2020222888 A AU 2020222888A AU 2020222888 A AU2020222888 A AU 2020222888A AU 2020222888 B2 AU2020222888 B2 AU 2020222888B2
- Authority
- AU
- Australia
- Prior art keywords
- nucleic acid
- beads
- bead
- primer
- partitions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/149—Particles, e.g. beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962804082P | 2019-02-11 | 2019-02-11 | |
| US62/804,082 | 2019-02-11 | ||
| US201962890240P | 2019-08-22 | 2019-08-22 | |
| US62/890,240 | 2019-08-22 | ||
| US201962916683P | 2019-10-17 | 2019-10-17 | |
| US62/916,683 | 2019-10-17 | ||
| PCT/US2020/017491 WO2020167656A1 (en) | 2019-02-11 | 2020-02-10 | Methods for nucleic acid analysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2020222888A1 AU2020222888A1 (en) | 2021-09-30 |
| AU2020222888B2 true AU2020222888B2 (en) | 2024-08-01 |
Family
ID=72045127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020222888A Active AU2020222888B2 (en) | 2019-02-11 | 2020-02-10 | Methods for nucleic acid analysis |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US12319959B2 (enExample) |
| EP (1) | EP3924515A4 (enExample) |
| JP (1) | JP7528103B2 (enExample) |
| KR (1) | KR20210138017A (enExample) |
| CN (1) | CN113728113A (enExample) |
| AU (1) | AU2020222888B2 (enExample) |
| CA (1) | CA3129315A1 (enExample) |
| MX (1) | MX2021009482A (enExample) |
| SG (1) | SG11202108563QA (enExample) |
| WO (1) | WO2020167656A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3129315A1 (en) | 2019-02-11 | 2020-08-20 | Ultima Genomics, Inc. | Methods for nucleic acid analysis |
| KR20230052952A (ko) * | 2020-08-21 | 2023-04-20 | 울티마 제노믹스, 인크. | 표면 증폭을 위한 조성물 및 이의 용도 |
| AU2022328558A1 (en) * | 2021-08-20 | 2024-04-04 | Ultima Genomics, Inc. | Systems and methods for sample preparation for sequencing |
| WO2023081883A2 (en) * | 2021-11-08 | 2023-05-11 | Ultima Genomics, Inc. | Methylation sequencing methods and compositions |
| WO2025072163A1 (en) * | 2023-09-25 | 2025-04-03 | Ultima Genomics, Inc. | Systems and methods for processing analytes |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9388459B2 (en) | 2002-06-17 | 2016-07-12 | Affymetrix, Inc. | Methods for genotyping |
| CA2513899C (en) | 2003-01-29 | 2013-03-26 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| US8048627B2 (en) * | 2003-07-05 | 2011-11-01 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| AU2005216549A1 (en) | 2004-02-27 | 2005-09-09 | President And Fellows Of Harvard College | Polony fluorescent in situ sequencing beads |
| US8034568B2 (en) | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
| WO2010003132A1 (en) | 2008-07-02 | 2010-01-07 | Illumina Cambridge Ltd. | Using populations of beads for the fabrication of arrays on surfaces |
| JP5457222B2 (ja) | 2009-02-25 | 2014-04-02 | エフ．ホフマン−ラ ロシュ アーゲー | 小型化ハイスループット核酸分析 |
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US20120156728A1 (en) | 2010-12-17 | 2012-06-21 | Life Technologies Corporation | Clonal amplification of nucleic acid on solid surface with template walking |
| US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| NZ610129A (en) | 2010-10-04 | 2014-08-29 | Genapsys Inc | Systems and methods for automated reusable parallel biological reactions |
| US20130040365A1 (en) | 2011-08-10 | 2013-02-14 | Life Technologies Corporation | Polymerase compositions, methods of making and using same |
| AU2013249123A1 (en) | 2012-04-19 | 2014-12-11 | Life Technologies Corporation | Method of performing digital PCR |
| EP3461910B1 (en) | 2012-04-19 | 2020-08-26 | Life Technologies Corporation | Nucleic acid amplification |
| SG10201802883UA (en) | 2012-04-19 | 2018-05-30 | Life Technologies Corp | Nucleic acid amplification |
| US8895249B2 (en) * | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| WO2014151961A1 (en) | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | Matrix arrays and methods for making same |
| EP3611262B1 (en) * | 2013-03-15 | 2020-11-11 | Lineage Biosciences, Inc. | Methods of sequencing the immune repertoire |
| US10160995B2 (en) * | 2013-05-13 | 2018-12-25 | Qiagen Waltham, Inc. | Analyte enrichment methods and compositions |
| AU2014302277A1 (en) * | 2013-06-27 | 2015-12-24 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| BR112016023625A2 (pt) | 2014-04-10 | 2018-06-26 | 10X Genomics, Inc. | dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos |
| WO2015191815A1 (en) | 2014-06-13 | 2015-12-17 | Life Technologies Corporation | Multiplex nucleic acid amplification |
| GB201414745D0 (en) | 2014-08-19 | 2014-10-01 | Articzymes As | Exonucleases |
| WO2016187466A1 (en) * | 2015-05-20 | 2016-11-24 | Invenra, Inc. | Methods, apparatus and compositions for expression and high throughput screening |
| CA3002740A1 (en) | 2015-10-19 | 2017-04-27 | Dovetail Genomics, Llc | Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection |
| JP7064439B2 (ja) * | 2015-11-04 | 2022-05-10 | アトレカ インコーポレイテッド | 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット |
| CN108431223A (zh) * | 2016-01-08 | 2018-08-21 | 生物辐射实验室股份有限公司 | 每液滴分辨率下的多个珠 |
| EP3440220B1 (en) | 2016-04-07 | 2020-03-25 | Illumina, Inc. | Methods and systems for construction of normalized nucleic acid libraries |
| US10544456B2 (en) | 2016-07-20 | 2020-01-28 | Genapsys, Inc. | Systems and methods for nucleic acid sequencing |
| WO2018064297A1 (en) | 2016-09-29 | 2018-04-05 | Ultima Genomics, Inc. | Systems and methods for nucleic acid sequencing |
| US10011872B1 (en) * | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| JP2018183097A (ja) | 2017-04-26 | 2018-11-22 | 株式会社エンプラス | 細胞由来の解析用液滴の単離方法、および細胞の解析方法 |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| WO2019079653A1 (en) | 2017-10-19 | 2019-04-25 | Ultima Genomics, Inc. | METHODS OF TREATING APPARATUSED END SEQUENCES |
| EP4310196A3 (en) | 2017-11-07 | 2024-04-24 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| US12226745B2 (en) | 2017-11-07 | 2025-02-18 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| SG11202007686VA (en) * | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| CA3129315A1 (en) | 2019-02-11 | 2020-08-20 | Ultima Genomics, Inc. | Methods for nucleic acid analysis |
| KR20260033107A (ko) | 2019-02-19 | 2026-03-10 | 울티마 제노믹스, 인크. | 광학 검출 및 시퀀싱을 위한 링커 및 방법 |
| KR20230052952A (ko) | 2020-08-21 | 2023-04-20 | 울티마 제노믹스, 인크. | 표면 증폭을 위한 조성물 및 이의 용도 |
| WO2022217112A1 (en) | 2021-04-09 | 2022-10-13 | Ultima Genomics, Inc. | Systems and methods for spatial screening of analytes |
-
2020
- 2020-02-10 CA CA3129315A patent/CA3129315A1/en active Pending
- 2020-02-10 CN CN202080027939.0A patent/CN113728113A/zh active Pending
- 2020-02-10 AU AU2020222888A patent/AU2020222888B2/en active Active
- 2020-02-10 KR KR1020217028674A patent/KR20210138017A/ko not_active Ceased
- 2020-02-10 MX MX2021009482A patent/MX2021009482A/es unknown
- 2020-02-10 SG SG11202108563QA patent/SG11202108563QA/en unknown
- 2020-02-10 WO PCT/US2020/017491 patent/WO2020167656A1/en not_active Ceased
- 2020-02-10 EP EP20755711.7A patent/EP3924515A4/en active Pending
- 2020-02-10 JP JP2021546249A patent/JP7528103B2/ja active Active
-
2021
- 2021-08-05 US US17/394,692 patent/US12319959B2/en active Active
-
2025
- 2025-07-28 US US19/282,712 patent/US20260078438A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| MX2021009482A (es) | 2021-11-12 |
| SG11202108563QA (en) | 2021-09-29 |
| JP7528103B2 (ja) | 2024-08-05 |
| AU2020222888A1 (en) | 2021-09-30 |
| EP3924515A1 (en) | 2021-12-22 |
| US12319959B2 (en) | 2025-06-03 |
| WO2020167656A1 (en) | 2020-08-20 |
| US20220042072A1 (en) | 2022-02-10 |
| JP2022519324A (ja) | 2022-03-22 |
| KR20210138017A (ko) | 2021-11-18 |
| CA3129315A1 (en) | 2020-08-20 |
| CN113728113A (zh) | 2021-11-30 |
| US20260078438A1 (en) | 2026-03-19 |
| EP3924515A4 (en) | 2022-08-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |