MX2021009482A - Metodos para el analisis de acidos nucleicos. - Google Patents

Metodos para el analisis de acidos nucleicos.

Info

Publication number
MX2021009482A
MX2021009482A MX2021009482A MX2021009482A MX2021009482A MX 2021009482 A MX2021009482 A MX 2021009482A MX 2021009482 A MX2021009482 A MX 2021009482A MX 2021009482 A MX2021009482 A MX 2021009482A MX 2021009482 A MX2021009482 A MX 2021009482A
Authority
MX
Mexico
Prior art keywords
nucleic acid
methods
beads
acid analysis
clonal amplification
Prior art date
Application number
MX2021009482A
Other languages
English (en)
Inventor
Gilad Almogy
Chandan Shee
Florian Oberstrass
Omer Barad
Original Assignee
Ultima Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ultima Genomics Inc filed Critical Ultima Genomics Inc
Publication of MX2021009482A publication Critical patent/MX2021009482A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente descripción proporciona métodos y procesos para incrementar la eficiencia y la exactitud del secuenciamiento de ácidos nucleicos, utilizando técnicas tales como la reacción en cadena de la polimerasa (PCR). Los métodos descritos en el presente documento pueden utilizarse para lograr la amplificación clonal, incluso con una distribución mayor que la distribución de Poisson de las perlas y/o plantillas de los ácidos nucleicos en una emulsión. Un método de PCR puede comprender generar una división (por ejemplo, una gota), que comprende al menos dos perlas y/o al menos dos moléculas de ácido nucleico y generar los productos de la amplificación clonal que corresponden a la molécula de ácido nucleico, al menos un subconjunto de los cuales puede unirse a una perla.
MX2021009482A 2019-02-11 2020-02-10 Metodos para el analisis de acidos nucleicos. MX2021009482A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962804082P 2019-02-11 2019-02-11
US201962890240P 2019-08-22 2019-08-22
US201962916683P 2019-10-17 2019-10-17
PCT/US2020/017491 WO2020167656A1 (en) 2019-02-11 2020-02-10 Methods for nucleic acid analysis

Publications (1)

Publication Number Publication Date
MX2021009482A true MX2021009482A (es) 2021-11-12

Family

ID=72045127

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021009482A MX2021009482A (es) 2019-02-11 2020-02-10 Metodos para el analisis de acidos nucleicos.

Country Status (10)

Country Link
US (1) US20220042072A1 (es)
EP (1) EP3924515A4 (es)
JP (1) JP2022519324A (es)
KR (1) KR20210138017A (es)
CN (1) CN113728113A (es)
AU (1) AU2020222888A1 (es)
CA (1) CA3129315A1 (es)
MX (1) MX2021009482A (es)
SG (1) SG11202108563QA (es)
WO (1) WO2020167656A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023081883A2 (en) * 2021-11-08 2023-05-11 Ultima Genomics, Inc. Methylation sequencing methods and compositions

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004069849A2 (en) * 2003-01-29 2004-08-19 454 Corporation Bead emulsion nucleic acid amplification
JP5183063B2 (ja) * 2003-07-05 2013-04-17 ザ ジョンズ ホプキンス ユニバーシティ 遺伝的変異の検出および列挙のための方法ならびに組成物
BR112015032512A8 (pt) * 2013-06-27 2020-01-07 10X Genomics Inc método de implementação de código de barras de materiais de amostra
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
EP3155127B1 (en) * 2014-06-13 2020-07-22 Life Technologies Corporation Multiplex nucleic acid amplification
WO2016187466A1 (en) * 2015-05-20 2016-11-24 Invenra, Inc. Methods, apparatus and compositions for expression and high throughput screening
US10730030B2 (en) * 2016-01-08 2020-08-04 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
US10011872B1 (en) * 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP3752832A1 (en) * 2018-02-12 2020-12-23 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations

Also Published As

Publication number Publication date
EP3924515A4 (en) 2022-08-24
EP3924515A1 (en) 2021-12-22
SG11202108563QA (en) 2021-09-29
WO2020167656A1 (en) 2020-08-20
CA3129315A1 (en) 2020-08-20
CN113728113A (zh) 2021-11-30
AU2020222888A1 (en) 2021-09-30
JP2022519324A (ja) 2022-03-22
US20220042072A1 (en) 2022-02-10
KR20210138017A (ko) 2021-11-18

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