JPWO2020028631A5 - - Google Patents
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- Publication number
- JPWO2020028631A5 JPWO2020028631A5 JP2021505400A JP2021505400A JPWO2020028631A5 JP WO2020028631 A5 JPWO2020028631 A5 JP WO2020028631A5 JP 2021505400 A JP2021505400 A JP 2021505400A JP 2021505400 A JP2021505400 A JP 2021505400A JP WO2020028631 A5 JPWO2020028631 A5 JP WO2020028631A5
- Authority
- JP
- Japan
- Prior art keywords
- detection probe
- seq
- oligomer
- amplification
- probe oligomer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims description 187
- 230000003321 amplification Effects 0.000 claims description 158
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 158
- 238000001514 detection method Methods 0.000 claims description 127
- 125000003729 nucleotide group Chemical group 0.000 claims description 110
- 150000007523 nucleic acids Chemical class 0.000 claims description 87
- 239000002773 nucleotide Substances 0.000 claims description 83
- 241000701044 Human gammaherpesvirus 4 Species 0.000 claims description 72
- 108020004707 nucleic acids Proteins 0.000 claims description 72
- 102000039446 nucleic acids Human genes 0.000 claims description 72
- 230000000295 complement effect Effects 0.000 claims description 47
- 238000006467 substitution reaction Methods 0.000 claims description 33
- 238000000034 method Methods 0.000 claims description 31
- 238000006243 chemical reaction Methods 0.000 claims description 27
- 239000011541 reaction mixture Substances 0.000 claims description 27
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 claims description 21
- 108091034117 Oligonucleotide Proteins 0.000 claims description 20
- 238000000338 in vitro Methods 0.000 claims description 19
- 230000002452 interceptive effect Effects 0.000 claims description 9
- 241000701022 Cytomegalovirus Species 0.000 claims description 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 6
- 108060002716 Exonuclease Proteins 0.000 claims description 6
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 102000013165 exonuclease Human genes 0.000 claims description 6
- 238000012408 PCR amplification Methods 0.000 claims description 3
- 108010006785 Taq Polymerase Proteins 0.000 claims description 3
- 210000004369 blood Anatomy 0.000 claims description 3
- 239000008280 blood Substances 0.000 claims description 3
- 229940104302 cytosine Drugs 0.000 claims description 3
- 230000009977 dual effect Effects 0.000 claims description 3
- 230000007062 hydrolysis Effects 0.000 claims description 3
- 238000006460 hydrolysis reaction Methods 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 46
- 108020004414 DNA Proteins 0.000 description 38
- 108091028043 Nucleic acid sequence Proteins 0.000 description 25
- 101150059079 EBNA1 gene Proteins 0.000 description 10
- 239000000203 mixture Substances 0.000 description 10
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
- 238000009396 hybridization Methods 0.000 description 6
- 230000000692 anti-sense effect Effects 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
- 238000009472 formulation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- RXNXLAHQOVLMIE-UHFFFAOYSA-N phenyl 10-methylacridin-10-ium-9-carboxylate Chemical compound C12=CC=CC=C2[N+](C)=C2C=CC=CC2=C1C(=O)OC1=CC=CC=C1 RXNXLAHQOVLMIE-UHFFFAOYSA-N 0.000 description 2
- -1 U.S. Pat. No. 5 Chemical class 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862713330P | 2018-08-01 | 2018-08-01 | |
| US62/713,330 | 2018-08-01 | ||
| PCT/US2019/044614 WO2020028631A1 (en) | 2018-08-01 | 2019-08-01 | Compositions and methods for detecting nucleic acids of epstein-barr virus |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021532787A JP2021532787A (ja) | 2021-12-02 |
| JPWO2020028631A5 true JPWO2020028631A5 (fi) | 2024-01-16 |
| JP7469290B2 JP7469290B2 (ja) | 2024-04-16 |
Family
ID=67614706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021505400A Active JP7469290B2 (ja) | 2018-08-01 | 2019-08-01 | エプスタイン－バールウイルスの核酸を検出するための組成物および方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20210332449A1 (fi) |
| EP (1) | EP3830302B1 (fi) |
| JP (1) | JP7469290B2 (fi) |
| AU (1) | AU2019314449A1 (fi) |
| CA (1) | CA3106975A1 (fi) |
| WO (1) | WO2020028631A1 (fi) |
Family Cites Families (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2422956A1 (fr) | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
| US4786600A (en) | 1984-05-25 | 1988-11-22 | The Trustees Of Columbia University In The City Of New York | Autocatalytic replication of recombinant RNA |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4868105A (en) | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| CA1308372C (en) | 1986-08-11 | 1992-10-06 | Geneva Ruth Davis | Nucleic acid probe sandwich assay methods and compositions |
| US5541308A (en) | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| JP2774121B2 (ja) | 1987-07-31 | 1998-07-09 | ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ | 標的ポリヌクレオチド配列の選択的増幅 |
| US5283174A (en) | 1987-09-21 | 1994-02-01 | Gen-Probe, Incorporated | Homogenous protection assay |
| US5639604A (en) | 1987-09-21 | 1997-06-17 | Gen-Probe Incorporated | Homogeneous protection assay |
| ATE124143T1 (de) | 1987-09-21 | 1995-07-15 | Gen Probe Inc | Homogener abschirmungstest. |
| US5585481A (en) | 1987-09-21 | 1996-12-17 | Gen-Probe Incorporated | Linking reagents for nucleotide probes |
| US5185439A (en) | 1987-10-05 | 1993-02-09 | Gen-Probe Incorporated | Acridinium ester labelling and purification of nucleotide probes |
| US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| US5118801A (en) | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| US5656207A (en) | 1989-06-24 | 1997-08-12 | Gen Probe Incorporated | Detecting or quantifying multiple analytes using labelling techniques |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| WO1993013121A1 (en) | 1991-12-24 | 1993-07-08 | Isis Pharmaceuticals, Inc. | Gapped 2' modified oligonucleotides |
| US5378825A (en) | 1990-07-27 | 1995-01-03 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogs |
| US5849481A (en) | 1990-07-27 | 1998-12-15 | Chiron Corporation | Nucleic acid hybridization assays employing large comb-type branched polynucleotides |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5578444A (en) * | 1991-06-27 | 1996-11-26 | Genelabs Technologies, Inc. | Sequence-directed DNA-binding molecules compositions and methods |
| US5424413A (en) | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
| CA2135073C (en) | 1992-05-06 | 2002-11-19 | Daniel L. Kacian | Nucleic acid sequence amplification method, composition and kit |
| JP3360737B2 (ja) * | 1992-05-13 | 2002-12-24 | 株式会社ヤトロン | エプスタイン−バールウイルスの検出方法 |
| JP4372837B2 (ja) | 1992-08-04 | 2009-11-25 | ジェン−プローブ・インコーポレイテッド | 核酸配列増幅 |
| US5422252A (en) | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
| KR100189229B1 (ko) | 1993-07-23 | 1999-06-01 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 | 핵산 증폭을 강화하는 방법 |
| JPH0779776A (ja) * | 1993-09-16 | 1995-03-28 | Toyobo Co Ltd | エプスタイン・バールウイルス（ｅｂｖ）検出用オリゴヌクレオチドおよびその用途 |
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| US7888458B1 (en) * | 1993-11-30 | 2011-02-15 | John B. Harley | Diagnostics and therapy of epstein-barr virus in autoimmune disorders |
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| EP0709466B1 (en) | 1994-10-28 | 2006-09-27 | Gen-Probe Incorporated | Compositions and methods for the simultaneous detection and quantification of multiple specific nucleic acid sequences |
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| ES2271994T3 (es) | 1997-05-02 | 2007-04-16 | Gen-Probe Incorporated | Hibridacion en dos pasos y captura de un polinucleotido. |
| US6180340B1 (en) | 1997-10-31 | 2001-01-30 | Gen-Probe Incorporated | Extended dynamic range assays |
| JPH11137300A (ja) * | 1997-11-07 | 1999-05-25 | Srl:Kk | リアルタイム検出ｐｃｒ法によるｅｂウイルス遺伝子の測定方法並びにそれに用いられるプライマー及びプローブ |
| DE69940770D1 (de) * | 1998-03-04 | 2009-06-04 | Biomerieux Bv | Vervielfältigung und nachweis von ebv barf1 zur diagnose von nasopharyngealen karzinom |
| ATE440963T1 (de) | 1998-07-02 | 2009-09-15 | Gen Probe Inc | Molekulare fackeln |
| DE60140317D1 (de) * | 2000-12-14 | 2009-12-10 | Gen Probe Inc | Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden |
| US6753137B2 (en) * | 2001-01-31 | 2004-06-22 | The Chinese University Of Hong Kong | Circulating epstein-barr virus DNA in the serum of patients with gastric carcinoma |
| DE60324810D1 (de) | 2002-09-20 | 2009-01-02 | New England Biolabs Inc | HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN |
| US8034554B2 (en) | 2004-07-01 | 2011-10-11 | Gen-Probe Incorporated | Methods and compositions to detect nucleic acids in a biological sample |
| CA2957197C (en) | 2004-08-27 | 2017-12-12 | Gen-Probe Incorporated | Single-primer nucleic acid amplification methods |
| JP2008531052A (ja) | 2005-02-28 | 2008-08-14 | ジェン−プローブ・インコーポレーテッド | 核酸ハイブリダイゼーションスイッチプローブを用いて被分析体を検出するための組成物及び方法 |
| JP5205609B2 (ja) * | 2006-06-06 | 2013-06-05 | 国立大学法人名古屋大学 | ウイルス検出用オリゴヌクレオチドセット、ｅｂｖ、ｃｍｖ及びｈｈｖ−６の分析方法及び検出キット |
| US9051601B2 (en) | 2006-08-01 | 2015-06-09 | Gen-Probe Incorporated | Methods of nonspecific target capture of nucleic acids |
| ES2399502T3 (es) * | 2010-07-13 | 2013-04-01 | Cnrs | Procedimiento para el aislamiento de las proteínas unidas a cualquier tipo de secuencia de ácido nucleico de interés |
| CA2925792C (en) * | 2013-10-04 | 2023-01-24 | Advance Sentry Corporation | Methods and devices for nasopharyngeal carcinoma screening |
-
2019
- 2019-08-01 AU AU2019314449A patent/AU2019314449A1/en active Pending
- 2019-08-01 EP EP19752862.3A patent/EP3830302B1/en active Active
- 2019-08-01 US US17/264,574 patent/US20210332449A1/en active Pending
- 2019-08-01 CA CA3106975A patent/CA3106975A1/en active Pending
- 2019-08-01 JP JP2021505400A patent/JP7469290B2/ja active Active
- 2019-08-01 WO PCT/US2019/044614 patent/WO2020028631A1/en unknown
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