JPWO2019180137A5 - - Google Patents
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- Publication number
- JPWO2019180137A5 JPWO2019180137A5 JP2020544940A JP2020544940A JPWO2019180137A5 JP WO2019180137 A5 JPWO2019180137 A5 JP WO2019180137A5 JP 2020544940 A JP2020544940 A JP 2020544940A JP 2020544940 A JP2020544940 A JP 2020544940A JP WO2019180137 A5 JPWO2019180137 A5 JP WO2019180137A5
- Authority
- JP
- Japan
- Prior art keywords
- dna polymerase
- amino acid
- primer
- seq
- dntp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 18
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 18
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 5
- 150000001413 amino acids Chemical class 0.000 claims 5
- 102000040430 polynucleotide Human genes 0.000 claims 4
- 108091033319 polynucleotide Proteins 0.000 claims 4
- 239000002157 polynucleotide Substances 0.000 claims 4
- 239000011541 reaction mixture Substances 0.000 claims 4
- 125000003275 alpha amino acid group Chemical group 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 235000011178 triphosphate Nutrition 0.000 claims 2
- 239000001226 triphosphate Substances 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 1
- 230000010354 integration Effects 0.000 claims 1
- 239000002777 nucleoside Substances 0.000 claims 1
- -1 nucleoside triphosphate Chemical class 0.000 claims 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862645935P | 2018-03-21 | 2018-03-21 | |
| US62/645,935 | 2018-03-21 | ||
| PCT/EP2019/057080 WO2019180137A1 (en) | 2018-03-21 | 2019-03-21 | Dna polymerases for efficient and effective incorporation of methylated-dntps |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2021516544A JP2021516544A (ja) | 2021-07-08 |
| JPWO2019180137A5 true JPWO2019180137A5 (https=) | 2022-03-31 |
| JP2021516544A5 JP2021516544A5 (https=) | 2022-03-31 |
| JP7319990B2 JP7319990B2 (ja) | 2023-08-02 |
Family
ID=65995680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020544940A Active JP7319990B2 (ja) | 2018-03-21 | 2019-03-21 | メチル化ｄＮＴＰを効率的かつ効果的に組み込むＤＮＡポリメラーゼ |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US12043851B2 (https=) |
| EP (1) | EP3768832B1 (https=) |
| JP (1) | JP7319990B2 (https=) |
| CN (1) | CN111868238B (https=) |
| ES (1) | ES2969883T3 (https=) |
| WO (1) | WO2019180137A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114574465B (zh) * | 2022-05-09 | 2022-08-05 | 上海众启生物科技有限公司 | 一种聚合酶突变体及其应用 |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4666848A (en) | 1984-08-31 | 1987-05-19 | Cetus Corporation | Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5693502A (en) | 1990-06-11 | 1997-12-02 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| KR100484124B1 (ko) | 1994-10-17 | 2005-09-08 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 변형된 뉴클레오티드 결합부위를 갖는 dna 서열화용 dna 폴리머라제 |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| EP0834569A1 (en) | 1996-10-03 | 1998-04-08 | Roche Diagnostics GmbH | Thermostable DNA polymerase from carboxydothermus hydrogenoformans |
| US5858671A (en) | 1996-11-01 | 1999-01-12 | The University Of Iowa Research Foundation | Iterative and regenerative DNA sequencing method |
| ATE280177T1 (de) | 1997-03-20 | 2004-11-15 | Hoffmann La Roche | Modifizierte primer |
| US6228628B1 (en) | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| EP0921196A1 (en) | 1997-12-02 | 1999-06-09 | Roche Diagnostics GmbH | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction |
| US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
| AU2001241621B2 (en) * | 2000-02-23 | 2005-08-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination |
| US7179590B2 (en) | 2000-04-18 | 2007-02-20 | Roche Molecular Systems, Inc | High temperature reverse transcription using mutant DNA polymerases |
| GB0016258D0 (en) | 2000-07-03 | 2000-08-23 | Amersham Pharm Biotech Uk Ltd | Base analogues |
| US7052839B2 (en) | 2001-08-29 | 2006-05-30 | Amersham Biosciences Corp | Terminal-phosphate-labeled nucleotides and methods of use |
| US7256019B2 (en) | 2001-08-29 | 2007-08-14 | Ge Healthcare Bio-Sciences Corp. | Terminal phosphate blocked nucleoside polyphosphates |
| US7148049B2 (en) | 2002-04-02 | 2006-12-12 | Roche Molecular Systems, Inc. | Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity |
| US7947817B2 (en) | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| US7572581B2 (en) | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| US20060172324A1 (en) | 2005-01-28 | 2006-08-03 | Roche Molecular Systems, Inc. | Methods of genotyping using differences in melting temperature |
| GB0509508D0 (en) | 2005-05-10 | 2005-06-15 | Solexa Ltd | Improved polymerases |
| US20110294168A1 (en) | 2006-10-18 | 2011-12-01 | Roche Molecular Systems, Inc. | Dna polymerases and related methods |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8208381B2 (en) | 2007-07-27 | 2012-06-26 | Eg Innovations Pte. Ltd. | Root-cause approach to problem diagnosis in data networks |
| US9193959B2 (en) | 2010-04-16 | 2015-11-24 | Roche Diagnostics Operations, Inc. | T7 RNA polymerase variants with enhanced thermostability |
| KR101622442B1 (ko) | 2010-06-18 | 2016-05-19 | 에프. 호프만-라 로슈 아게 | ３’―미스매치 구별이 증가된 ｄｎａ 중합효소 |
| WO2013013822A1 (en) | 2011-07-28 | 2013-01-31 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
| JP6140182B2 (ja) | 2011-12-08 | 2017-05-31 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
| JP6144697B2 (ja) | 2011-12-08 | 2017-06-07 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
| WO2013083262A1 (en) | 2011-12-08 | 2013-06-13 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
| WO2014081511A1 (en) | 2012-11-21 | 2014-05-30 | Courtagen Life Sciences Inc. | Method for preventing carry-over contamination in nucleic acid amplification reactions |
| CA2888148C (en) | 2012-12-13 | 2019-01-15 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
-
2019
- 2019-03-21 ES ES19714567T patent/ES2969883T3/es active Active
- 2019-03-21 WO PCT/EP2019/057080 patent/WO2019180137A1/en not_active Ceased
- 2019-03-21 EP EP19714567.5A patent/EP3768832B1/en active Active
- 2019-03-21 CN CN201980020293.0A patent/CN111868238B/zh active Active
- 2019-03-21 JP JP2020544940A patent/JP7319990B2/ja active Active
- 2019-03-21 US US16/981,397 patent/US12043851B2/en active Active
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