JPWO2020053327A5 - - Google Patents
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- Publication number
- JPWO2020053327A5 JPWO2020053327A5 JP2021514033A JP2021514033A JPWO2020053327A5 JP WO2020053327 A5 JPWO2020053327 A5 JP WO2020053327A5 JP 2021514033 A JP2021514033 A JP 2021514033A JP 2021514033 A JP2021514033 A JP 2021514033A JP WO2020053327 A5 JPWO2020053327 A5 JP WO2020053327A5
- Authority
- JP
- Japan
- Prior art keywords
- dna polymerase
- seq
- amino acid
- primer
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 14
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 14
- 238000000034 method Methods 0.000 claims 6
- 230000035772 mutation Effects 0.000 claims 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims 4
- 150000001413 amino acids Chemical class 0.000 claims 4
- 238000006073 displacement reaction Methods 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 239000002777 nucleoside Substances 0.000 claims 3
- -1 nucleoside triphosphates Chemical class 0.000 claims 3
- 108091033319 polynucleotide Proteins 0.000 claims 3
- 102000040430 polynucleotide Human genes 0.000 claims 3
- 239000002157 polynucleotide Substances 0.000 claims 3
- 239000001226 triphosphate Substances 0.000 claims 3
- 235000011178 triphosphate Nutrition 0.000 claims 3
- 102000004533 Endonucleases Human genes 0.000 claims 2
- 108010042407 Endonucleases Proteins 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 102000004214 DNA polymerase A Human genes 0.000 claims 1
- 108090000725 DNA polymerase A Proteins 0.000 claims 1
- 108060002716 Exonuclease Proteins 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 230000014102 antigen processing and presentation of exogenous peptide antigen via MHC class I Effects 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 102000013165 exonuclease Human genes 0.000 claims 1
- 238000011901 isothermal amplification Methods 0.000 claims 1
- 230000001404 mediated effect Effects 0.000 claims 1
- 239000011541 reaction mixture Substances 0.000 claims 1
- 108010068698 spleen exonuclease Proteins 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862730908P | 2018-09-13 | 2018-09-13 | |
| US62/730,908 | 2018-09-13 | ||
| PCT/EP2019/074320 WO2020053327A1 (en) | 2018-09-13 | 2019-09-12 | Mutant dna polymerase(s) with improved strand displacement ability |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2022500046A JP2022500046A (ja) | 2022-01-04 |
| JP2022500046A5 JP2022500046A5 (https=) | 2022-09-14 |
| JPWO2020053327A5 true JPWO2020053327A5 (https=) | 2022-09-14 |
| JP7504872B2 JP7504872B2 (ja) | 2024-06-24 |
Family
ID=67988974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021514033A Active JP7504872B2 (ja) | 2018-09-13 | 2019-09-12 | 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US11739306B2 (https=) |
| EP (1) | EP3850086A1 (https=) |
| JP (1) | JP7504872B2 (https=) |
| CN (1) | CN112703248B (https=) |
| WO (1) | WO2020053327A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113817708B (zh) * | 2021-11-23 | 2022-02-18 | 中国医学科学院北京协和医院 | 一种重组dna聚合酶及其制备方法和应用 |
| CN114574465B (zh) * | 2022-05-09 | 2022-08-05 | 上海众启生物科技有限公司 | 一种聚合酶突变体及其应用 |
| CN116334026B (zh) * | 2023-04-03 | 2026-01-02 | 安序源生物科技(深圳)有限公司 | 一种突变型dna聚合酶及其制备方法与应用 |
Family Cites Families (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4666848A (en) | 1984-08-31 | 1987-05-19 | Cetus Corporation | Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| WO1992006200A1 (en) | 1990-09-28 | 1992-04-16 | F. Hoffmann-La-Roche Ag | 5' to 3' exonuclease mutations of thermostable dna polymerases |
| US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5693502A (en) | 1990-06-11 | 1997-12-02 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| EP0871775A4 (en) | 1995-09-08 | 2002-07-31 | Life Technologies Inc | CLONED DNA POLYMERASES FROM THERMOTOGA AND MUTANTS THEREOF |
| EP0834569A1 (en) | 1996-10-03 | 1998-04-08 | Roche Diagnostics GmbH | Thermostable DNA polymerase from carboxydothermus hydrogenoformans |
| US6228628B1 (en) | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| CA2243985C (en) | 1997-09-11 | 2004-08-10 | F. Hoffmann-La Roche Ag | Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes |
| EP0921196A1 (en) | 1997-12-02 | 1999-06-09 | Roche Diagnostics GmbH | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction |
| ATE286981T1 (de) | 1999-09-28 | 2005-01-15 | Roche Diagnostics Gmbh | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
| US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
| JP2001269188A (ja) | 2000-03-11 | 2001-10-02 | Roche Diagnostics Gmbh | Ｐｃｒにおける性能が向上したｂ型ｄｎａポリメラーゼ変異体 |
| US7179590B2 (en) | 2000-04-18 | 2007-02-20 | Roche Molecular Systems, Inc | High temperature reverse transcription using mutant DNA polymerases |
| CA2409775C (en) | 2001-12-03 | 2010-07-13 | F. Hoffmann-La Roche Ag | Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro |
| US7148049B2 (en) | 2002-04-02 | 2006-12-12 | Roche Molecular Systems, Inc. | Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity |
| US7947817B2 (en) | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| US7572581B2 (en) | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| US20060172324A1 (en) | 2005-01-28 | 2006-08-03 | Roche Molecular Systems, Inc. | Methods of genotyping using differences in melting temperature |
| WO2007076461A1 (en) * | 2005-12-22 | 2007-07-05 | Ge Healthcare Bio-Sciences Corp. | Thermostable dna polymerase from thermus scotoductus |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8026091B2 (en) * | 2007-07-13 | 2011-09-27 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| JP5926246B2 (ja) | 2010-06-18 | 2016-05-25 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| WO2012110060A1 (en) * | 2011-02-15 | 2012-08-23 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| EP2697372B1 (en) * | 2011-04-11 | 2015-12-16 | Roche Diagnostics GmbH | Dna polymerases with improved activity |
| JP6144697B2 (ja) | 2011-12-08 | 2017-06-07 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
| EP2777843B1 (de) | 2013-03-15 | 2015-09-30 | ZF Friedrichshafen AG | Verfahren und Vorrichtung für das Verfahren zur Herstellung eines Gusswerkstücks |
| WO2014161712A1 (en) | 2013-04-05 | 2014-10-09 | Bioron Gmbh | Novel dna-ploymerases |
-
2019
- 2019-09-12 JP JP2021514033A patent/JP7504872B2/ja active Active
- 2019-09-12 US US17/274,667 patent/US11739306B2/en active Active
- 2019-09-12 WO PCT/EP2019/074320 patent/WO2020053327A1/en not_active Ceased
- 2019-09-12 CN CN201980059768.7A patent/CN112703248B/zh active Active
- 2019-09-12 EP EP19769752.7A patent/EP3850086A1/en active Pending
-
2023
- 2023-07-06 US US18/219,045 patent/US12286651B2/en active Active
-
2025
- 2025-03-24 US US19/088,724 patent/US20250283061A1/en active Pending
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