CN112703248B - 具有改良链置换能力的突变型dna聚合酶 - Google Patents

具有改良链置换能力的突变型dna聚合酶 Download PDF

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Publication number
CN112703248B
CN112703248B CN201980059768.7A CN201980059768A CN112703248B CN 112703248 B CN112703248 B CN 112703248B CN 201980059768 A CN201980059768 A CN 201980059768A CN 112703248 B CN112703248 B CN 112703248B
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seq
dna polymerase
amino acid
polymerase
dna
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CN112703248A (zh
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F·库尔巴诺夫
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201980059768.7A 2018-09-13 2019-09-12 具有改良链置换能力的突变型dna聚合酶 Active CN112703248B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862730908P 2018-09-13 2018-09-13
US62/730908 2018-09-13
PCT/EP2019/074320 WO2020053327A1 (en) 2018-09-13 2019-09-12 Mutant dna polymerase(s) with improved strand displacement ability

Publications (2)

Publication Number Publication Date
CN112703248A CN112703248A (zh) 2021-04-23
CN112703248B true CN112703248B (zh) 2025-03-25

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US (3) US11739306B2 (https=)
EP (1) EP3850086A1 (https=)
JP (1) JP7504872B2 (https=)
CN (1) CN112703248B (https=)
WO (1) WO2020053327A1 (https=)

Families Citing this family (3)

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Publication number Priority date Publication date Assignee Title
CN113817708B (zh) * 2021-11-23 2022-02-18 中国医学科学院北京协和医院 一种重组dna聚合酶及其制备方法和应用
CN114574465B (zh) * 2022-05-09 2022-08-05 上海众启生物科技有限公司 一种聚合酶突变体及其应用
CN116334026B (zh) * 2023-04-03 2026-01-02 安序源生物科技(深圳)有限公司 一种突变型dna聚合酶及其制备方法与应用

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN102099464A (zh) * 2007-07-13 2011-06-15 霍夫曼-拉罗奇有限公司 突变型dna聚合酶及相关方法

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EP0834569A1 (en) 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostable DNA polymerase from carboxydothermus hydrogenoformans
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
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EP0921196A1 (en) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
ATE286981T1 (de) 1999-09-28 2005-01-15 Roche Diagnostics Gmbh Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation
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JP2001269188A (ja) 2000-03-11 2001-10-02 Roche Diagnostics Gmbh Ｐｃｒにおける性能が向上したｂ型ｄｎａポリメラーゼ変異体
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US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
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JP5926246B2 (ja) 2010-06-18 2016-05-25 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
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US11739306B2 (en) 2023-08-29
JP2022500046A (ja) 2022-01-04
US20240110162A1 (en) 2024-04-04
US12286651B2 (en) 2025-04-29
US20250283061A1 (en) 2025-09-11
US20210269779A1 (en) 2021-09-02
WO2020053327A1 (en) 2020-03-19
EP3850086A1 (en) 2021-07-21
JP7504872B2 (ja) 2024-06-24
CN112703248A (zh) 2021-04-23

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