JP7504872B2 - 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ - Google Patents

改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ Download PDF

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JP7504872B2
JP7504872B2 JP2021514033A JP2021514033A JP7504872B2 JP 7504872 B2 JP7504872 B2 JP 7504872B2 JP 2021514033 A JP2021514033 A JP 2021514033A JP 2021514033 A JP2021514033 A JP 2021514033A JP 7504872 B2 JP7504872 B2 JP 7504872B2
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seq
amino acid
dna polymerase
dna
polymerase
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JP2022500046A (ja
JP2022500046A5 (https=
JPWO2020053327A5 (https=
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クルバノフ フェルツ
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2021514033A 2018-09-13 2019-09-12 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ Active JP7504872B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862730908P 2018-09-13 2018-09-13
US62/730,908 2018-09-13
PCT/EP2019/074320 WO2020053327A1 (en) 2018-09-13 2019-09-12 Mutant dna polymerase(s) with improved strand displacement ability

Publications (4)

Publication Number Publication Date
JP2022500046A JP2022500046A (ja) 2022-01-04
JP2022500046A5 JP2022500046A5 (https=) 2022-09-14
JPWO2020053327A5 JPWO2020053327A5 (https=) 2022-09-14
JP7504872B2 true JP7504872B2 (ja) 2024-06-24

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JP2021514033A Active JP7504872B2 (ja) 2018-09-13 2019-09-12 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ

Country Status (5)

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US (3) US11739306B2 (https=)
EP (1) EP3850086A1 (https=)
JP (1) JP7504872B2 (https=)
CN (1) CN112703248B (https=)
WO (1) WO2020053327A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113817708B (zh) * 2021-11-23 2022-02-18 中国医学科学院北京协和医院 一种重组dna聚合酶及其制备方法和应用
CN114574465B (zh) * 2022-05-09 2022-08-05 上海众启生物科技有限公司 一种聚合酶突变体及其应用
CN116334026B (zh) * 2023-04-03 2026-01-02 安序源生物科技(深圳)有限公司 一种突变型dna聚合酶及其制备方法与应用

Citations (4)

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Publication number Priority date Publication date Assignee Title
WO2007076461A1 (en) 2005-12-22 2007-07-05 Ge Healthcare Bio-Sciences Corp. Thermostable dna polymerase from thermus scotoductus
JP2014509193A (ja) 2011-02-15 2014-04-17 エフ．ホフマン−ラ ロシュ アーゲー 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
JP2014511701A (ja) 2011-04-11 2014-05-19 エフ．ホフマン−ラ ロシュ アーゲー 改良された活性を有するｄｎａポリメラーゼ
WO2014161712A1 (en) 2013-04-05 2014-10-09 Bioron Gmbh Novel dna-ploymerases

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US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4666848A (en) 1984-08-31 1987-05-19 Cetus Corporation Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
WO1992006200A1 (en) 1990-09-28 1992-04-16 F. Hoffmann-La-Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
EP0871775A4 (en) 1995-09-08 2002-07-31 Life Technologies Inc CLONED DNA POLYMERASES FROM THERMOTOGA AND MUTANTS THEREOF
EP0834569A1 (en) 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostable DNA polymerase from carboxydothermus hydrogenoformans
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
CA2243985C (en) 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
EP0921196A1 (en) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
ATE286981T1 (de) 1999-09-28 2005-01-15 Roche Diagnostics Gmbh Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
JP2001269188A (ja) 2000-03-11 2001-10-02 Roche Diagnostics Gmbh Ｐｃｒにおける性能が向上したｂ型ｄｎａポリメラーゼ変異体
US7179590B2 (en) 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7572581B2 (en) 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US20060172324A1 (en) 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8026091B2 (en) * 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods
JP5926246B2 (ja) 2010-06-18 2016-05-25 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
EP2777843B1 (de) 2013-03-15 2015-09-30 ZF Friedrichshafen AG Verfahren und Vorrichtung für das Verfahren zur Herstellung eines Gusswerkstücks

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007076461A1 (en) 2005-12-22 2007-07-05 Ge Healthcare Bio-Sciences Corp. Thermostable dna polymerase from thermus scotoductus
JP2014509193A (ja) 2011-02-15 2014-04-17 エフ．ホフマン−ラ ロシュ アーゲー 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
JP2014511701A (ja) 2011-04-11 2014-05-19 エフ．ホフマン−ラ ロシュ アーゲー 改良された活性を有するｄｎａポリメラーゼ
WO2014161712A1 (en) 2013-04-05 2014-10-09 Bioron Gmbh Novel dna-ploymerases

Also Published As

Publication number Publication date
US11739306B2 (en) 2023-08-29
JP2022500046A (ja) 2022-01-04
CN112703248B (zh) 2025-03-25
US20240110162A1 (en) 2024-04-04
US12286651B2 (en) 2025-04-29
US20250283061A1 (en) 2025-09-11
US20210269779A1 (en) 2021-09-02
WO2020053327A1 (en) 2020-03-19
EP3850086A1 (en) 2021-07-21
CN112703248A (zh) 2021-04-23

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