JPWO2019180137A5 - - Google Patents
Download PDFInfo
- Publication number
- JPWO2019180137A5 JPWO2019180137A5 JP2020544940A JP2020544940A JPWO2019180137A5 JP WO2019180137 A5 JPWO2019180137 A5 JP WO2019180137A5 JP 2020544940 A JP2020544940 A JP 2020544940A JP 2020544940 A JP2020544940 A JP 2020544940A JP WO2019180137 A5 JPWO2019180137 A5 JP WO2019180137A5
- Authority
- JP
- Japan
- Prior art keywords
- dna polymerase
- amino acid
- primer
- seq
- dntp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Claims (15)
配列番号２の６４０位に対応する前記ＤＮＡポリメラーゼのアミノ酸はＭであり、
前記対照のＤＮＡポリメラーゼは、前記ＤＮＡポリメラーゼと同一のアミノ酸配列を有するが、配列番号２の６４０位に対応する前記対照のＤＮＡポリメラーゼのアミノ酸はＩである、ＤＮＡポリメラーゼ。 A DNA polymerase having at least 90% sequence identity with SEQ ID NO: 2 and having improved integration efficiency of methylated deoxynucleotide triphosphate (dNTP) as compared to a control DNA polymerase.
The amino acid of the DNA polymerase corresponding to position 640 of SEQ ID NO: 2 is M.
The control DNA polymerase has the same amino acid sequence as the DNA polymerase, but the amino acid of the control DNA polymerase corresponding to position 640 of SEQ ID NO: 2 is I, a DNA polymerase.
（ｂ）ｄＮＴＰを提供する容器、および
（ｃ）プライマー伸長に適する緩衝液を提供する容器
からなる群から選択される１個以上の追加の容器をさらに含む、請求項８に記載のキット。 (A) A container that provides a primer that can hybridize to a predetermined polynucleotide template under primer extension conditions.
The kit of claim 8 , further comprising one or more additional containers selected from the group consisting of (b) a container providing dNTPs and (c) a container providing a buffer suitable for primer extension.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862645935P | 2018-03-21 | 2018-03-21 | |
| US62/645,935 | 2018-03-21 | ||
| PCT/EP2019/057080 WO2019180137A1 (en) | 2018-03-21 | 2019-03-21 | Dna polymerases for efficient and effective incorporation of methylated-dntps |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021516544A JP2021516544A (en) | 2021-07-08 |
| JPWO2019180137A5 true JPWO2019180137A5 (en) | 2022-03-31 |
| JP7319990B2 JP7319990B2 (en) | 2023-08-02 |
Family
ID=65995680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020544940A Active JP7319990B2 (en) | 2018-03-21 | 2019-03-21 | DNA polymerases that efficiently and effectively incorporate methylated dNTPs |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20210032609A1 (en) |
| EP (1) | EP3768832B1 (en) |
| JP (1) | JP7319990B2 (en) |
| CN (1) | CN111868238A (en) |
| ES (1) | ES2969883T3 (en) |
| WO (1) | WO2019180137A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114574465B (en) * | 2022-05-09 | 2022-08-05 | 上海众启生物科技有限公司 | Polymerase mutant and application thereof |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4666848A (en) | 1984-08-31 | 1987-05-19 | Cetus Corporation | Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5693502A (en) | 1990-06-11 | 1997-12-02 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| KR100484124B1 (en) * | 1994-10-17 | 2005-09-08 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | DNA Polymerases Having Modified Nucleotide Binding Site for DNA Sequencing |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| EP0834569A1 (en) | 1996-10-03 | 1998-04-08 | Roche Diagnostics GmbH | Thermostable DNA polymerase from carboxydothermus hydrogenoformans |
| EP0866071B1 (en) | 1997-03-20 | 2004-10-20 | F. Hoffmann-La Roche Ag | Modified primers |
| US6228628B1 (en) | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| EP0921196A1 (en) | 1997-12-02 | 1999-06-09 | Roche Diagnostics GmbH | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction |
| US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
| AU2001241621B2 (en) * | 2000-02-23 | 2005-08-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination |
| US7179590B2 (en) | 2000-04-18 | 2007-02-20 | Roche Molecular Systems, Inc | High temperature reverse transcription using mutant DNA polymerases |
| US7256019B2 (en) | 2001-08-29 | 2007-08-14 | Ge Healthcare Bio-Sciences Corp. | Terminal phosphate blocked nucleoside polyphosphates |
| US7148049B2 (en) | 2002-04-02 | 2006-12-12 | Roche Molecular Systems, Inc. | Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity |
| US7947817B2 (en) | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| US7572581B2 (en) | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| US20060172324A1 (en) | 2005-01-28 | 2006-08-03 | Roche Molecular Systems, Inc. | Methods of genotyping using differences in melting temperature |
| GB0509508D0 (en) * | 2005-05-10 | 2005-06-15 | Solexa Ltd | Improved polymerases |
| US20110294168A1 (en) | 2006-10-18 | 2011-12-01 | Roche Molecular Systems, Inc. | Dna polymerases and related methods |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8208381B2 (en) | 2007-07-27 | 2012-06-26 | Eg Innovations Pte. Ltd. | Root-cause approach to problem diagnosis in data networks |
| US9193959B2 (en) * | 2010-04-16 | 2015-11-24 | Roche Diagnostics Operations, Inc. | T7 RNA polymerase variants with enhanced thermostability |
| JP5926248B2 (en) * | 2010-06-18 | 2016-05-25 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | DNA polymerase with increased 3 'terminal mismatch discrimination |
| ES2569723T3 (en) * | 2011-12-08 | 2016-05-12 | F. Hoffmann-La Roche Ag | DNA polymerases with enhanced activity |
| ES2716277T3 (en) * | 2011-12-08 | 2019-06-11 | Hoffmann La Roche | DNA polymerases with enhanced activity |
| JP6144697B2 (en) | 2011-12-08 | 2017-06-07 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | DNA polymerase with improved activity |
| JP6356694B2 (en) * | 2012-12-13 | 2018-07-11 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | DNA polymerase with improved activity |
-
2019
- 2019-03-21 CN CN201980020293.0A patent/CN111868238A/en active Pending
- 2019-03-21 JP JP2020544940A patent/JP7319990B2/en active Active
- 2019-03-21 EP EP19714567.5A patent/EP3768832B1/en active Active
- 2019-03-21 ES ES19714567T patent/ES2969883T3/en active Active
- 2019-03-21 US US16/981,397 patent/US20210032609A1/en active Pending
- 2019-03-21 WO PCT/EP2019/057080 patent/WO2019180137A1/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10619190B2 (en) | Compositions, methods, systems and kits for target nucleic acid enrichment | |
| Craw et al. | Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review | |
| NO340891B1 (en) | Double specificity oligonucleotide | |
| JP3867926B2 (en) | Nucleic acid amplification method | |
| US20030157549A1 (en) | Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules | |
| JP6455864B2 (en) | Polynucleotide and use thereof | |
| EP2099936B1 (en) | Improved multiplex nucleic acid amplification using blocked primers | |
| JP2010532979A5 (en) | ||
| JP6021230B2 (en) | Method for quantifying human DNA using internal controls | |
| JP2005521422A5 (en) | ||
| WO2006074334A2 (en) | Identification of rna targets using helicases | |
| JP2018516594A5 (en) | ||
| US20200224260A1 (en) | Method for suppressing non-specific amplification products in nucleic acid amplification technologies | |
| US20240084363A1 (en) | Method for quantifying and/or detecting human male dna | |
| CN1313893A (en) | FY7 polymerase | |
| JPWO2019180137A5 (en) | ||
| US7501254B2 (en) | Methods and compositions for amplification and capture of nucleic acid sequences | |
| US9657337B2 (en) | Reaction buffer for microarray | |
| WO2005083120A1 (en) | Method for amplifying members of a gene family | |
| US9988659B1 (en) | In vivo conversion of nucleosides in plasmid DNA | |
| JP4942160B2 (en) | Method for isothermal amplification of nucleic acid using RecA protein | |
| JPWO2020053327A5 (en) | ||
| JPWO2020234200A5 (en) | ||
| KR101096570B1 (en) | Oligonucleotides for Detection of Dandruff-associated the Yeasts of the Genus Malassezia | |
| WO1999037820A1 (en) | An exponential nucleic acid amplification method using a single primer of opposing polarity |