CN111868238B - 用于甲基化-dNTPs的效率高的和有效参入的DNA聚合酶 - Google Patents

用于甲基化-dNTPs的效率高的和有效参入的DNA聚合酶 Download PDF

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CN111868238B
CN111868238B CN201980020293.0A CN201980020293A CN111868238B CN 111868238 B CN111868238 B CN 111868238B CN 201980020293 A CN201980020293 A CN 201980020293A CN 111868238 B CN111868238 B CN 111868238B
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dna polymerase
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CN111868238A (zh
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K·A·鲍尔
B·埃克特
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201980020293.0A 2018-03-21 2019-03-21 用于甲基化-dNTPs的效率高的和有效参入的DNA聚合酶 Active CN111868238B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862645935P 2018-03-21 2018-03-21
US62/645935 2018-03-21
PCT/EP2019/057080 WO2019180137A1 (en) 2018-03-21 2019-03-21 Dna polymerases for efficient and effective incorporation of methylated-dntps

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CN111868238A CN111868238A (zh) 2020-10-30
CN111868238B true CN111868238B (zh) 2024-06-11

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US (1) US12043851B2 (https=)
EP (1) EP3768832B1 (https=)
JP (1) JP7319990B2 (https=)
CN (1) CN111868238B (https=)
ES (1) ES2969883T3 (https=)
WO (1) WO2019180137A1 (https=)

Families Citing this family (1)

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Publication number Priority date Publication date Assignee Title
CN114574465B (zh) * 2022-05-09 2022-08-05 上海众启生物科技有限公司 一种聚合酶突变体及其应用

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KR100484124B1 (ko) * 1994-10-17 2005-09-08 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 변형된 뉴클레오티드 결합부위를 갖는 dna 서열화용 dna 폴리머라제
CN101180390A (zh) * 2005-05-10 2008-05-14 索莱克萨有限公司 改进的聚合酶
WO2013083263A1 (en) * 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
CN103998603A (zh) * 2011-12-08 2014-08-20 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶

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WO2001062975A2 (en) * 2000-02-23 2001-08-30 City Of Hope Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination
CN101180390A (zh) * 2005-05-10 2008-05-14 索莱克萨有限公司 改进的聚合酶
WO2013083263A1 (en) * 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
CN103987844A (zh) * 2011-12-08 2014-08-13 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶
CN103998603A (zh) * 2011-12-08 2014-08-20 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶

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Also Published As

Publication number Publication date
EP3768832B1 (en) 2023-11-29
CN111868238A (zh) 2020-10-30
JP2021516544A (ja) 2021-07-08
US12043851B2 (en) 2024-07-23
US20210032609A1 (en) 2021-02-04
JP7319990B2 (ja) 2023-08-02
WO2019180137A1 (en) 2019-09-26
EP3768832A1 (en) 2021-01-27
ES2969883T3 (es) 2024-05-23

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