JP7319990B2 - メチル化ｄＮＴＰを効率的かつ効果的に組み込むＤＮＡポリメラーゼ - Google Patents

メチル化ｄＮＴＰを効率的かつ効果的に組み込むＤＮＡポリメラーゼ Download PDF

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JP7319990B2
JP7319990B2 JP2020544940A JP2020544940A JP7319990B2 JP 7319990 B2 JP7319990 B2 JP 7319990B2 JP 2020544940 A JP2020544940 A JP 2020544940A JP 2020544940 A JP2020544940 A JP 2020544940A JP 7319990 B2 JP7319990 B2 JP 7319990B2
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dna polymerase
amino acid
dna
seq
polymerase
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JP2021516544A (ja
JPWO2019180137A5 (https=
JP2021516544A5 (https=
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エー．バウアー キース
エッカート バーバラ
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2020544940A 2018-03-21 2019-03-21 メチル化ｄＮＴＰを効率的かつ効果的に組み込むＤＮＡポリメラーゼ Active JP7319990B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862645935P 2018-03-21 2018-03-21
US62/645,935 2018-03-21
PCT/EP2019/057080 WO2019180137A1 (en) 2018-03-21 2019-03-21 Dna polymerases for efficient and effective incorporation of methylated-dntps

Publications (4)

Publication Number Publication Date
JP2021516544A JP2021516544A (ja) 2021-07-08
JPWO2019180137A5 JPWO2019180137A5 (https=) 2022-03-31
JP2021516544A5 JP2021516544A5 (https=) 2022-03-31
JP7319990B2 true JP7319990B2 (ja) 2023-08-02

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JP2020544940A Active JP7319990B2 (ja) 2018-03-21 2019-03-21 メチル化ｄＮＴＰを効率的かつ効果的に組み込むＤＮＡポリメラーゼ

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US (1) US12043851B2 (https=)
EP (1) EP3768832B1 (https=)
JP (1) JP7319990B2 (https=)
CN (1) CN111868238B (https=)
ES (1) ES2969883T3 (https=)
WO (1) WO2019180137A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114574465B (zh) * 2022-05-09 2022-08-05 上海众启生物科技有限公司 一种聚合酶突变体及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015505674A (ja) 2011-12-08 2015-02-26 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ

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US4666848A (en) 1984-08-31 1987-05-19 Cetus Corporation Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
KR100484124B1 (ko) 1994-10-17 2005-09-08 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 변형된 뉴클레오티드 결합부위를 갖는 dna 서열화용 dna 폴리머라제
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EP0834569A1 (en) 1996-10-03 1998-04-08 Roche Diagnostics GmbH Thermostable DNA polymerase from carboxydothermus hydrogenoformans
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ATE280177T1 (de) 1997-03-20 2004-11-15 Hoffmann La Roche Modifizierte primer
US6228628B1 (en) 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
EP0921196A1 (en) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
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AU2001241621B2 (en) * 2000-02-23 2005-08-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination
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Patent Citations (1)

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JP2015505674A (ja) 2011-12-08 2015-02-26 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ

Also Published As

Publication number Publication date
EP3768832B1 (en) 2023-11-29
CN111868238A (zh) 2020-10-30
JP2021516544A (ja) 2021-07-08
US12043851B2 (en) 2024-07-23
US20210032609A1 (en) 2021-02-04
WO2019180137A1 (en) 2019-09-26
EP3768832A1 (en) 2021-01-27
ES2969883T3 (es) 2024-05-23
CN111868238B (zh) 2024-06-11

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