JP2025081508A5 - - Google Patents
Info
- Publication number
- JP2025081508A5 JP2025081508A5 JP2025024821A JP2025024821A JP2025081508A5 JP 2025081508 A5 JP2025081508 A5 JP 2025081508A5 JP 2025024821 A JP2025024821 A JP 2025024821A JP 2025024821 A JP2025024821 A JP 2025024821A JP 2025081508 A5 JP2025081508 A5 JP 2025081508A5
- Authority
- JP
- Japan
- Prior art keywords
- discrimination
- increased
- dna polymerase
- taq dna
- rhpcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361904335P | 2013-11-14 | 2013-11-14 | |
| US61/904,335 | 2013-11-14 | ||
| JP2019138063A JP2019216723A (ja) | 2013-11-14 | 2019-07-26 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
| JP2021125170A JP7266645B2 (ja) | 2013-11-14 | 2021-07-30 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
| JP2023067018A JP7640606B2 (ja) | 2013-11-14 | 2023-04-17 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023067018A Division JP7640606B2 (ja) | 2013-11-14 | 2023-04-17 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2025081508A JP2025081508A (ja) | 2025-05-27 |
| JP2025081508A5 true JP2025081508A5 (https=) | 2025-10-21 |
Family
ID=52016863
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016530836A Pending JP2016536995A (ja) | 2013-11-14 | 2014-11-14 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
| JP2019138063A Pending JP2019216723A (ja) | 2013-11-14 | 2019-07-26 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
| JP2021125170A Active JP7266645B2 (ja) | 2013-11-14 | 2021-07-30 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
| JP2023067018A Active JP7640606B2 (ja) | 2013-11-14 | 2023-04-17 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
| JP2025024821A Pending JP2025081508A (ja) | 2013-11-14 | 2025-02-19 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
Family Applications Before (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016530836A Pending JP2016536995A (ja) | 2013-11-14 | 2014-11-14 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
| JP2019138063A Pending JP2019216723A (ja) | 2013-11-14 | 2019-07-26 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
| JP2021125170A Active JP7266645B2 (ja) | 2013-11-14 | 2021-07-30 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
| JP2023067018A Active JP7640606B2 (ja) | 2013-11-14 | 2023-04-17 | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US20150191707A1 (https=) |
| EP (1) | EP3068876B1 (https=) |
| JP (5) | JP2016536995A (https=) |
| AU (1) | AU2014348323B2 (https=) |
| CA (2) | CA3207128A1 (https=) |
| WO (1) | WO2015073931A1 (https=) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016536995A (ja) * | 2013-11-14 | 2016-12-01 | インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
| WO2017062965A1 (en) * | 2015-10-08 | 2017-04-13 | Arizona Board Of Regents On Behalf Of Arizona State University | Highly sensitive optical sensor for polymerase screening |
| CN116064748A (zh) * | 2015-11-25 | 2023-05-05 | 综合基因技术公司 | 用于变体检测的方法 |
| US20210395799A1 (en) * | 2015-11-25 | 2021-12-23 | Integrated Dna Technologies, Inc. | Methods for variant detection |
| WO2018044831A1 (en) | 2016-08-30 | 2018-03-08 | Integrated Dna Technologies, Inc. | Cleavable hairpin primers |
| EP3610030B1 (en) * | 2017-04-13 | 2024-08-07 | Integrated DNA Technologies, Inc. | Methods for variant detection |
| CN110603326B (zh) * | 2017-09-26 | 2024-03-08 | 宁波尚宁生物技术有限公司 | 扩增靶核酸的方法 |
| AU2019373208A1 (en) * | 2018-10-29 | 2021-05-13 | Codexis, Inc. | Engineered DNA polymerase variants |
| US11613738B2 (en) * | 2019-05-14 | 2023-03-28 | Abclonal Science, Inc. | Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye |
| WO2021242041A1 (ko) * | 2020-05-29 | 2021-12-02 | (주)제노텍 | 유전자 변이에 대한 구분성이 향상된 dna 중합효소 변이체 |
| KR20230124946A (ko) * | 2020-12-24 | 2023-08-28 | 인티그레이티드 디엔에이 테크놀로지스 아이엔씨. | 고-충실도 DNA 폴리머라아제를 이용한 rhPCR 기반 앰플리콘시퀀싱에서 프라이머 이량체 및 오프-타겟 증폭을 감소시키는 RNAse H2 돌연변이체 |
| CN114685671B (zh) * | 2020-12-30 | 2023-09-26 | 深圳华大生命科学研究院 | 特异性结合Taq DNA聚合酶的单克隆抗体及其应用 |
| US20240287481A1 (en) * | 2021-03-19 | 2024-08-29 | Mgi Tech Co., Ltd. | Polymerases for efficient incorporation of nucleotides with 3'-phosphate and other 3'-terminators |
| WO2022271682A1 (en) * | 2021-06-21 | 2022-12-29 | Amazon Technologies, Inc. | Polymerases for isothermal nucleic acid amplification |
| WO2023034814A1 (en) * | 2021-09-03 | 2023-03-09 | Singular Genomics Systems, Inc. | Methods for differentiating modified nucleobases |
| US12129495B2 (en) | 2021-10-15 | 2024-10-29 | Codexis, Inc. | Engineered DNA polymerase variants |
| CN116200362B (zh) * | 2021-11-30 | 2025-12-05 | 广州达安基因股份有限公司 | Taq酶突变体及其制备方法和用途 |
| WO2024040060A1 (en) * | 2022-08-16 | 2024-02-22 | 10X Genomics, Inc. | Ap50 polymerases and uses thereof |
| CA3267928A1 (en) * | 2022-09-23 | 2024-03-28 | Bio-Rad Laboratories, Inc. | RNASEH2-ACTIVATED UNIVERSAL FLUORESCENT PROBES |
| CN118109436B (zh) * | 2022-11-30 | 2025-04-01 | 深圳市新产业生物医学工程股份有限公司 | 一种突变型Taq DNA聚合酶及其试剂盒与应用 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| ATE138106T1 (de) | 1988-07-20 | 1996-06-15 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| US7150982B2 (en) * | 1991-09-09 | 2006-12-19 | Third Wave Technologies, Inc. | RNA detection assays |
| EP0803058B1 (en) | 1994-12-30 | 2008-11-05 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| DE69841023D1 (de) * | 1997-03-12 | 2009-09-10 | Applied Biosystems Llc | DNA Polymerasen mit verbesserten Fähigkeiten zum Einbau von markierten Nukleotiden |
| DE60027040T2 (de) | 1999-10-29 | 2006-11-23 | Stratagene California, La Jolla | Zusammensetzungen und methoden zur verwendung von dna polymerasen |
| US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
| EP1367136A4 (en) | 2001-02-15 | 2005-01-12 | Takara Bio Inc | METHOD FOR DETECTING NUCLEOTIDE POLYMORPHISM |
| US7629152B2 (en) * | 2002-03-01 | 2009-12-08 | Integrated Dna Technologies, Inc. | Methods for amplifying polymeric nucleic acids |
| DE60323611D1 (de) | 2002-03-01 | 2008-10-30 | Integrated Dna Tech Inc | Polynomiale amplifikation von nukleinsäuren |
| DE102004005885A1 (de) * | 2004-02-05 | 2005-10-13 | Rheinische Friedrich-Wilhelms-Universität Bonn | Mutierte DNA-Polymerasen mit erhöhter Fehlpaarungs-Diskriminierung |
| US20090068643A1 (en) | 2005-11-23 | 2009-03-12 | Integrated Dna Technologies, Inc. | Dual Function Primers for Amplifying DNA and Methods of Use |
| EP2644708B1 (en) * | 2008-04-30 | 2014-12-17 | Integrated Dna Technologies, Inc. | RNASE-H-based assays utilizing modified RNA monomers |
| US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| WO2010051434A1 (en) * | 2008-10-31 | 2010-05-06 | Helicos Biosciences Corporation | Polymerases and methods of use thereof |
| WO2011157436A1 (en) * | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| US8759062B2 (en) * | 2010-06-18 | 2014-06-24 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′- mismatch discrimination |
| JP5847200B2 (ja) * | 2011-02-15 | 2016-01-20 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| WO2012135053A2 (en) * | 2011-03-25 | 2012-10-04 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
| EP3046360A1 (en) | 2013-09-13 | 2016-07-20 | Fujitsu Limited | Method, base station, and user equipment for changing cell clusters |
| JP2016536995A (ja) * | 2013-11-14 | 2016-12-01 | インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド | 増大した鋳型識別活性を有するｄｎａポリメラーゼ突然変異体 |
-
2014
- 2014-11-14 JP JP2016530836A patent/JP2016536995A/ja active Pending
- 2014-11-14 CA CA3207128A patent/CA3207128A1/en active Pending
- 2014-11-14 EP EP14809730.6A patent/EP3068876B1/en active Active
- 2014-11-14 US US14/542,539 patent/US20150191707A1/en not_active Abandoned
- 2014-11-14 CA CA2930595A patent/CA2930595C/en active Active
- 2014-11-14 WO PCT/US2014/065845 patent/WO2015073931A1/en not_active Ceased
- 2014-11-14 AU AU2014348323A patent/AU2014348323B2/en active Active
-
2016
- 2016-12-21 US US15/386,631 patent/US10982247B2/en active Active
-
2019
- 2019-07-26 JP JP2019138063A patent/JP2019216723A/ja active Pending
-
2021
- 2021-03-22 US US17/208,286 patent/US20220010346A1/en active Pending
- 2021-07-30 JP JP2021125170A patent/JP7266645B2/ja active Active
-
2023
- 2023-04-17 JP JP2023067018A patent/JP7640606B2/ja active Active
-
2025
- 2025-02-19 JP JP2025024821A patent/JP2025081508A/ja active Pending
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