CA3207128A1 - Dna polymerase mutants having enhanced template discrimination activity - Google Patents

Dna polymerase mutants having enhanced template discrimination activity

Info

Publication number
CA3207128A1
CA3207128A1 CA3207128A CA3207128A CA3207128A1 CA 3207128 A1 CA3207128 A1 CA 3207128A1 CA 3207128 A CA3207128 A CA 3207128A CA 3207128 A CA3207128 A CA 3207128A CA 3207128 A1 CA3207128 A1 CA 3207128A1
Authority
CA
Canada
Prior art keywords
dna polymerase
discrimination
seq
taq dna
enhanced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3207128A
Other languages
English (en)
French (fr)
Inventor
Mark Aaron Behlke
Joseph A. Walder
Richard Owczarzy
Scott D. Rose
Joseph R. Dobosy
Susan M. Rupp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Integrated DNA Technologies Inc
Original Assignee
Integrated DNA Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrated DNA Technologies Inc filed Critical Integrated DNA Technologies Inc
Publication of CA3207128A1 publication Critical patent/CA3207128A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA3207128A 2013-11-14 2014-11-14 Dna polymerase mutants having enhanced template discrimination activity Pending CA3207128A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361904335P 2013-11-14 2013-11-14
US61/904,335 2013-11-14
CA2930595A CA2930595C (en) 2013-11-14 2014-11-14 Dna polymerase mutants having enhanced template discrimination activity

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2930595A Division CA2930595C (en) 2013-11-14 2014-11-14 Dna polymerase mutants having enhanced template discrimination activity

Publications (1)

Publication Number Publication Date
CA3207128A1 true CA3207128A1 (en) 2015-05-21

Family

ID=52016863

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3207128A Pending CA3207128A1 (en) 2013-11-14 2014-11-14 Dna polymerase mutants having enhanced template discrimination activity
CA2930595A Active CA2930595C (en) 2013-11-14 2014-11-14 Dna polymerase mutants having enhanced template discrimination activity

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2930595A Active CA2930595C (en) 2013-11-14 2014-11-14 Dna polymerase mutants having enhanced template discrimination activity

Country Status (6)

Country Link
US (3) US20150191707A1 (https=)
EP (1) EP3068876B1 (https=)
JP (5) JP2016536995A (https=)
AU (1) AU2014348323B2 (https=)
CA (2) CA3207128A1 (https=)
WO (1) WO2015073931A1 (https=)

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WO2017062965A1 (en) * 2015-10-08 2017-04-13 Arizona Board Of Regents On Behalf Of Arizona State University Highly sensitive optical sensor for polymerase screening
CN116064748A (zh) * 2015-11-25 2023-05-05 综合基因技术公司 用于变体检测的方法
US20210395799A1 (en) * 2015-11-25 2021-12-23 Integrated Dna Technologies, Inc. Methods for variant detection
WO2018044831A1 (en) 2016-08-30 2018-03-08 Integrated Dna Technologies, Inc. Cleavable hairpin primers
EP3610030B1 (en) * 2017-04-13 2024-08-07 Integrated DNA Technologies, Inc. Methods for variant detection
CN110603326B (zh) * 2017-09-26 2024-03-08 宁波尚宁生物技术有限公司 扩增靶核酸的方法
AU2019373208A1 (en) * 2018-10-29 2021-05-13 Codexis, Inc. Engineered DNA polymerase variants
US11613738B2 (en) * 2019-05-14 2023-03-28 Abclonal Science, Inc. Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye
WO2021242041A1 (ko) * 2020-05-29 2021-12-02 (주)제노텍 유전자 변이에 대한 구분성이 향상된 dna 중합효소 변이체
KR20230124946A (ko) * 2020-12-24 2023-08-28 인티그레이티드 디엔에이 테크놀로지스 아이엔씨. 고-충실도 DNA 폴리머라아제를 이용한 rhPCR 기반 앰플리콘시퀀싱에서 프라이머 이량체 및 오프-타겟 증폭을 감소시키는 RNAse H2 돌연변이체
CN114685671B (zh) * 2020-12-30 2023-09-26 深圳华大生命科学研究院 特异性结合Taq DNA聚合酶的单克隆抗体及其应用
US20240287481A1 (en) * 2021-03-19 2024-08-29 Mgi Tech Co., Ltd. Polymerases for efficient incorporation of nucleotides with 3'-phosphate and other 3'-terminators
WO2022271682A1 (en) * 2021-06-21 2022-12-29 Amazon Technologies, Inc. Polymerases for isothermal nucleic acid amplification
WO2023034814A1 (en) * 2021-09-03 2023-03-09 Singular Genomics Systems, Inc. Methods for differentiating modified nucleobases
US12129495B2 (en) 2021-10-15 2024-10-29 Codexis, Inc. Engineered DNA polymerase variants
CN116200362B (zh) * 2021-11-30 2025-12-05 广州达安基因股份有限公司 Taq酶突变体及其制备方法和用途
WO2024040060A1 (en) * 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
CA3267928A1 (en) * 2022-09-23 2024-03-28 Bio-Rad Laboratories, Inc. RNASEH2-ACTIVATED UNIVERSAL FLUORESCENT PROBES
CN118109436B (zh) * 2022-11-30 2025-04-01 深圳市新产业生物医学工程股份有限公司 一种突变型Taq DNA聚合酶及其试剂盒与应用

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Also Published As

Publication number Publication date
US20170114379A1 (en) 2017-04-27
AU2014348323B2 (en) 2020-09-24
AU2014348323A1 (en) 2016-06-02
US10982247B2 (en) 2021-04-20
EP3068876B1 (en) 2019-05-08
US20220010346A1 (en) 2022-01-13
CA2930595C (en) 2023-09-19
JP2025081508A (ja) 2025-05-27
JP7266645B2 (ja) 2023-04-28
CA2930595A1 (en) 2015-05-21
US20150191707A1 (en) 2015-07-09
JP2016536995A (ja) 2016-12-01
JP7640606B2 (ja) 2025-03-05
JP2019216723A (ja) 2019-12-26
JP2021177776A (ja) 2021-11-18
JP2023089188A (ja) 2023-06-27
EP3068876A1 (en) 2016-09-21
WO2015073931A1 (en) 2015-05-21

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