AU2014348323B2 - DNA polymerase mutants having enhanced template discrimination activity - Google Patents

DNA polymerase mutants having enhanced template discrimination activity Download PDF

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Publication number
AU2014348323B2
AU2014348323B2 AU2014348323A AU2014348323A AU2014348323B2 AU 2014348323 B2 AU2014348323 B2 AU 2014348323B2 AU 2014348323 A AU2014348323 A AU 2014348323A AU 2014348323 A AU2014348323 A AU 2014348323A AU 2014348323 B2 AU2014348323 B2 AU 2014348323B2
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Australia
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primer
mutant
seq
dna polymerase
dna
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AU2014348323A
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AU2014348323A1 (en
Inventor
Mark Aaron Behlke
Joseph R. Dobosy
Richard Owczarzy
Scott D. Rose
Susan M. Rupp
Joseph A. Walder
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Integrated DNA Technologies Inc
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Integrated DNA Technologies Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2014348323A 2013-11-14 2014-11-14 DNA polymerase mutants having enhanced template discrimination activity Active AU2014348323B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361904335P 2013-11-14 2013-11-14
US61/904,335 2013-11-14
PCT/US2014/065845 WO2015073931A1 (en) 2013-11-14 2014-11-14 Dna polymerase mutants having enhanced template discrimination activity

Publications (2)

Publication Number Publication Date
AU2014348323A1 AU2014348323A1 (en) 2016-06-02
AU2014348323B2 true AU2014348323B2 (en) 2020-09-24

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AU2014348323A Active AU2014348323B2 (en) 2013-11-14 2014-11-14 DNA polymerase mutants having enhanced template discrimination activity

Country Status (6)

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US (3) US20150191707A1 (https=)
EP (1) EP3068876B1 (https=)
JP (5) JP2016536995A (https=)
AU (1) AU2014348323B2 (https=)
CA (2) CA3207128A1 (https=)
WO (1) WO2015073931A1 (https=)

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WO2017062965A1 (en) * 2015-10-08 2017-04-13 Arizona Board Of Regents On Behalf Of Arizona State University Highly sensitive optical sensor for polymerase screening
CN116064748A (zh) * 2015-11-25 2023-05-05 综合基因技术公司 用于变体检测的方法
US20210395799A1 (en) * 2015-11-25 2021-12-23 Integrated Dna Technologies, Inc. Methods for variant detection
WO2018044831A1 (en) 2016-08-30 2018-03-08 Integrated Dna Technologies, Inc. Cleavable hairpin primers
EP3610030B1 (en) * 2017-04-13 2024-08-07 Integrated DNA Technologies, Inc. Methods for variant detection
CN110603326B (zh) * 2017-09-26 2024-03-08 宁波尚宁生物技术有限公司 扩增靶核酸的方法
AU2019373208A1 (en) * 2018-10-29 2021-05-13 Codexis, Inc. Engineered DNA polymerase variants
US11613738B2 (en) * 2019-05-14 2023-03-28 Abclonal Science, Inc. Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye
WO2021242041A1 (ko) * 2020-05-29 2021-12-02 (주)제노텍 유전자 변이에 대한 구분성이 향상된 dna 중합효소 변이체
KR20230124946A (ko) * 2020-12-24 2023-08-28 인티그레이티드 디엔에이 테크놀로지스 아이엔씨. 고-충실도 DNA 폴리머라아제를 이용한 rhPCR 기반 앰플리콘시퀀싱에서 프라이머 이량체 및 오프-타겟 증폭을 감소시키는 RNAse H2 돌연변이체
CN114685671B (zh) * 2020-12-30 2023-09-26 深圳华大生命科学研究院 特异性结合Taq DNA聚合酶的单克隆抗体及其应用
US20240287481A1 (en) * 2021-03-19 2024-08-29 Mgi Tech Co., Ltd. Polymerases for efficient incorporation of nucleotides with 3'-phosphate and other 3'-terminators
WO2022271682A1 (en) * 2021-06-21 2022-12-29 Amazon Technologies, Inc. Polymerases for isothermal nucleic acid amplification
WO2023034814A1 (en) * 2021-09-03 2023-03-09 Singular Genomics Systems, Inc. Methods for differentiating modified nucleobases
US12129495B2 (en) 2021-10-15 2024-10-29 Codexis, Inc. Engineered DNA polymerase variants
CN116200362B (zh) * 2021-11-30 2025-12-05 广州达安基因股份有限公司 Taq酶突变体及其制备方法和用途
WO2024040060A1 (en) * 2022-08-16 2024-02-22 10X Genomics, Inc. Ap50 polymerases and uses thereof
CA3267928A1 (en) * 2022-09-23 2024-03-28 Bio-Rad Laboratories, Inc. RNASEH2-ACTIVATED UNIVERSAL FLUORESCENT PROBES
CN118109436B (zh) * 2022-11-30 2025-04-01 深圳市新产业生物医学工程股份有限公司 一种突变型Taq DNA聚合酶及其试剂盒与应用

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WO2011157436A1 (en) * 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination

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Also Published As

Publication number Publication date
US20170114379A1 (en) 2017-04-27
AU2014348323A1 (en) 2016-06-02
US10982247B2 (en) 2021-04-20
EP3068876B1 (en) 2019-05-08
US20220010346A1 (en) 2022-01-13
CA2930595C (en) 2023-09-19
JP2025081508A (ja) 2025-05-27
JP7266645B2 (ja) 2023-04-28
CA2930595A1 (en) 2015-05-21
US20150191707A1 (en) 2015-07-09
JP2016536995A (ja) 2016-12-01
JP7640606B2 (ja) 2025-03-05
JP2019216723A (ja) 2019-12-26
JP2021177776A (ja) 2021-11-18
CA3207128A1 (en) 2015-05-21
JP2023089188A (ja) 2023-06-27
EP3068876A1 (en) 2016-09-21
WO2015073931A1 (en) 2015-05-21

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