JPWO2011149032A1 - 生体試料固定装置 - Google Patents

生体試料固定装置 Download PDF

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Publication number
JPWO2011149032A1
JPWO2011149032A1 JP2012517320A JP2012517320A JPWO2011149032A1 JP WO2011149032 A1 JPWO2011149032 A1 JP WO2011149032A1 JP 2012517320 A JP2012517320 A JP 2012517320A JP 2012517320 A JP2012517320 A JP 2012517320A JP WO2011149032 A1 JPWO2011149032 A1 JP WO2011149032A1
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Prior art keywords
biological sample
electrode
holding
holding portion
electrodes
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Japanese (ja)
Inventor
篤史 森本
篤史 森本
二見 達
達 二見
聡文 最上
聡文 最上
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Tosoh Corp
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Tosoh Corp
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44756Apparatus specially adapted therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00Separating dispersed particles from liquids by electrostatic effect
    • B03C5/005Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02Separators
    • B03C5/022Non-uniform field separators
    • B03C5/026Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277Apparatus
    • B01J2219/00279Features relating to reactor vessels
    • B01J2219/00306Reactor vessels in a multiple arrangement
    • B01J2219/00313Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315Microtiter plates
    • B01J2219/00317Microwell devices, i.e. having large numbers of wells
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00603Making arrays on substantially continuous surfaces
    • B01J2219/00646Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/00648Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00603Making arrays on substantially continuous surfaces
    • B01J2219/00653Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668Trapping microscopic beads
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0816Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0819Microarrays; Biochips
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424Dielectrophoretic forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00Details of magnetic or electrostatic separation
    • B03C2201/26Details of magnetic or electrostatic separation for use in medical applications

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Electrochemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Fluid Mechanics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2012517320A 2010-05-26 2011-05-26 生体試料固定装置 Pending JPWO2011149032A1 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
JP2010119984 2010-05-26
JP2010119995 2010-05-26
JP2010119995 2010-05-26
JP2010119984 2010-05-26
JP2010150523 2010-06-30
JP2010150523 2010-06-30
PCT/JP2011/062141 WO2011149032A1 (ja) 2010-05-26 2011-05-26 生体試料固定装置

Publications (1)

Publication Number Publication Date
JPWO2011149032A1 true JPWO2011149032A1 (ja) 2013-07-25

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JP2012517320A Pending JPWO2011149032A1 (ja) 2010-05-26 2011-05-26 生体試料固定装置

Country Status (4)

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US (1) US20130118905A1 (de)
EP (1) EP2578674A4 (de)
JP (1) JPWO2011149032A1 (de)
WO (1) WO2011149032A1 (de)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130099143A1 (en) * 2010-06-30 2013-04-25 Tosoh Corporation Structure for particle immobilization and apparatus for particle analysis
JP2012013551A (ja) * 2010-06-30 2012-01-19 Tosoh Corp 粒子固定用構造体、粒子解析装置、及び解析方法
JP2012013550A (ja) * 2010-06-30 2012-01-19 Tosoh Corp 粒子固定用構造体、粒子解析装置、及び解析方法
JP5720129B2 (ja) * 2010-06-30 2015-05-20 東ソー株式会社 粒子固定用構造体、粒子解析装置、及び解析方法
US9448157B2 (en) * 2012-07-18 2016-09-20 Sony Corporation Microparticle sorting apparatus, microchip for sorting microparticles and microparticle sorting method
US9857333B2 (en) * 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
AU2014340089B2 (en) 2013-10-22 2019-09-26 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
EP3060912B1 (de) 2013-10-22 2020-07-15 Berkeley Lights, Inc. Verfahren und mikrofluidische vorrichtung zum testen einer biologischen aktivität
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
US11318479B2 (en) 2013-12-18 2022-05-03 Berkeley Lights, Inc. Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
JP6583602B2 (ja) * 2014-09-19 2019-10-02 コニカミノルタ株式会社 細胞内の核酸の解析方法ならびにそのためのシステムおよびキット
JP6686361B2 (ja) * 2014-11-26 2020-04-22 東ソー株式会社 細胞回収のための標本作製方法
WO2016090295A1 (en) 2014-12-05 2016-06-09 The Regents Of The University Of California Single-sided light-actuated microfluidic device with integrated mesh ground
WO2016094333A1 (en) 2014-12-08 2016-06-16 Berkeley Lights, Inc. Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof
SG10201900567QA (en) 2014-12-08 2019-02-27 Berkeley Lights Inc Microfluidic device comprising lateral/vertical transistor structures and process of making and using same
CN113075113A (zh) 2014-12-09 2021-07-06 伯克利之光生命科技公司 微流体装置中微物体的自动检测和重新定位
CN107206377B (zh) 2014-12-09 2020-02-11 伯克利之光生命科技公司 微流体装置中测定阳性的区域的自动检测
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
CN209501708U (zh) 2014-12-10 2019-10-18 伯克利之光生命科技公司 用于操作电动装置的系统、显微镜、多输入光管及光传输系统
JP6421589B2 (ja) * 2014-12-22 2018-11-14 東ソー株式会社 微小粒子捕捉装置
JP6679873B2 (ja) * 2015-03-26 2020-04-15 東ソー株式会社 微小粒子保持装置
WO2016172623A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
SG11201708429WA (en) 2015-04-22 2017-11-29 Berkeley Lights Inc Microfluidic cell culture
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
AU2016252995B2 (en) 2015-04-22 2020-12-03 Berkeley Lights, Inc. Freezing and archiving cells on a microfluidic device
US10974246B2 (en) * 2015-06-08 2021-04-13 Japan Science And Technology Agency High-density micro-chamber array and measurement method using same
JP7337760B2 (ja) * 2015-10-07 2023-09-04 凸版印刷株式会社 生体分子の分析方法
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
IL299366A (en) 2015-11-23 2023-02-01 Berkeley Lights Inc Structures for microfluidic isolation are produced at the place of assembly, kits and their uses
JP7083752B2 (ja) 2015-12-08 2022-06-13 バークレー ライツ,インコーポレイテッド マイクロ流体デバイス、そのキット、及びその使用方法
JP6964590B2 (ja) 2015-12-30 2021-11-10 バークレー ライツ,インコーポレイテッド 光学的に駆動される対流及び変位のマイクロ流体デバイス、そのキット及び方法
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
EP3889176A1 (de) 2016-01-15 2021-10-06 Berkeley Lights, Inc. Verfahren zur herstellung patientenspezifischer antikrebstherapeutika und verfahren zur behandlung davon
CN109922885B (zh) 2016-03-16 2022-05-10 伯克利之光生命科技公司 用于基因组编辑克隆的选择和传代的方法、系统和装置
KR102614839B1 (ko) 2016-04-15 2023-12-15 버클리 라잇츠, 인크. 펜 내 분석들을 위한 방법들, 시스템들 및 키트들
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
SG10202008265XA (en) 2016-05-26 2020-09-29 Berkeley Lights Inc Covalently modified surfaces, kits, and methods of preparation and use
EP3487510B1 (de) 2016-07-21 2023-07-19 Berkeley Lights, Inc. Sortieren von t-lymphozyten in einer mikrofluidischen vorrichtung
EP3562594A4 (de) 2016-12-30 2020-09-09 The Regents of University of California Verfahren zur auswahl und erzeugung genomeditierten t-zellen
JP2018134026A (ja) * 2017-02-21 2018-08-30 東ソー株式会社 細胞保持方法
JPWO2018207454A1 (ja) * 2017-05-12 2020-03-19 株式会社ニコン 対象物操作装置、及び対象物操作方法
EP3721209B1 (de) 2017-10-15 2024-02-07 Berkeley Lights, Inc. Verfahren für in-pen-tests
JP7262220B2 (ja) * 2018-01-29 2023-04-21 東ソー株式会社 質量分析計を用いた粒子測定法
US11318469B2 (en) * 2018-04-25 2022-05-03 Hewlett-Packard Development Company, L.P. Selective release of material in thermally degradable capsule
JP7191370B2 (ja) * 2018-11-08 2022-12-19 国立大学法人 東京大学 細胞捕捉装置
CN114126762B (zh) 2019-04-30 2023-01-03 伯克利之光生命科技公司 用于包封和测定细胞的方法
JP7443762B2 (ja) 2019-12-25 2024-03-06 東ソー株式会社 試料中に含まれる細胞の保持方法
US20230191400A1 (en) * 2021-12-17 2023-06-22 Hidaca Ltd. Well array device, system and methods of use thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03502043A (ja) * 1987-10-09 1991-05-16 ベイラー カレッジ オブ メディシン 高周波電気パルスによる細胞ポレーションと細胞融合用の方法及び装置
US20050009004A1 (en) * 2002-05-04 2005-01-13 Jia Xu Apparatus including ion transport detecting structures and methods of use
JP2007296510A (ja) * 2006-04-03 2007-11-15 Tosoh Corp 微粒子操作装置及び微粒子操作方法
JP2008307454A (ja) * 2007-06-13 2008-12-25 Tosoh Corp 基板表面の親水化方法、親水性部材、それを用いた微粒子操作容器及び微粒子操作装置
JP2009273458A (ja) * 2008-04-17 2009-11-26 Tosoh Corp 細胞融合装置及び細胞融合方法
JP2010011824A (ja) * 2008-07-07 2010-01-21 Tosoh Corp 細胞融合容器、細胞融合装置及びこれらを用いた細胞融合方法
JP2010022360A (ja) * 2008-06-16 2010-02-04 Tosoh Corp 微粒子懸濁液導入容器とそれを用いた細胞融合容器、及び細胞融合装置

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US6149789A (en) * 1990-10-31 2000-11-21 Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Process for manipulating microscopic, dielectric particles and a device therefor
JP3097932B2 (ja) 1991-11-05 2000-10-10 株式会社アドバンス 静電クロマトグラフィー装置
CA2343873A1 (en) * 2000-04-12 2001-10-12 Wako Pure Chemical Industries Ltd. Electrode for dielectrophoretic apparatus, dielectrophoretic apparatus, methdo for manufacturing the same, and method for separating substances using the elctrode or dielectrophoretic apparatus
US20040011650A1 (en) * 2002-07-22 2004-01-22 Frederic Zenhausern Method and apparatus for manipulating polarizable analytes via dielectrophoresis
JP3723882B2 (ja) 2002-11-14 2005-12-07 篤 村口 抗原特異的リンパ球検出用マイクロウェルアレイチップ、抗原特異的リンパ球の検出法及び製造方法
JP2006284262A (ja) * 2005-03-31 2006-10-19 Sony Corp 正弦波電圧を重畳印加する物質間相互作用検出装置及び方法
JP4918811B2 (ja) 2005-06-13 2012-04-18 東ソー株式会社 細胞融合用チャンバーおよび細胞融合装置、並びにそれらを用いた細胞融合方法
US7686934B2 (en) * 2005-12-13 2010-03-30 Gamida For Life B.V. Three dimensional dielectrophoretic separator and methods of use
JP2009273459A (ja) * 2008-04-15 2009-11-26 Tosoh Corp 細胞選別装置、およびそれを用いた細胞選別方法
JP2011104487A (ja) * 2009-11-13 2011-06-02 Tosoh Corp 微粒子操作装置

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03502043A (ja) * 1987-10-09 1991-05-16 ベイラー カレッジ オブ メディシン 高周波電気パルスによる細胞ポレーションと細胞融合用の方法及び装置
US20050009004A1 (en) * 2002-05-04 2005-01-13 Jia Xu Apparatus including ion transport detecting structures and methods of use
JP2007296510A (ja) * 2006-04-03 2007-11-15 Tosoh Corp 微粒子操作装置及び微粒子操作方法
JP2008307454A (ja) * 2007-06-13 2008-12-25 Tosoh Corp 基板表面の親水化方法、親水性部材、それを用いた微粒子操作容器及び微粒子操作装置
JP2009273458A (ja) * 2008-04-17 2009-11-26 Tosoh Corp 細胞融合装置及び細胞融合方法
JP2010022360A (ja) * 2008-06-16 2010-02-04 Tosoh Corp 微粒子懸濁液導入容器とそれを用いた細胞融合容器、及び細胞融合装置
JP2010011824A (ja) * 2008-07-07 2010-01-21 Tosoh Corp 細胞融合容器、細胞融合装置及びこれらを用いた細胞融合方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JPN6011041015; 二見達 他: '高効率細胞融合技術の開発' 東ソー研究・技術報告 Vol.53, 2009, P.11-17 *

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