JPWO2005124321A1 - 測定装置 - Google Patents
測定装置 Download PDFInfo
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- JPWO2005124321A1 JPWO2005124321A1 JP2006514824A JP2006514824A JPWO2005124321A1 JP WO2005124321 A1 JPWO2005124321 A1 JP WO2005124321A1 JP 2006514824 A JP2006514824 A JP 2006514824A JP 2006514824 A JP2006514824 A JP 2006514824A JP WO2005124321 A1 JPWO2005124321 A1 JP WO2005124321A1
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- 230000003287 optical effect Effects 0.000 claims abstract description 80
- 238000001514 detection method Methods 0.000 claims abstract description 58
- 238000005259 measurement Methods 0.000 claims abstract description 39
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- 238000000034 method Methods 0.000 description 16
- 238000010586 diagram Methods 0.000 description 11
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- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 5
- 238000002060 fluorescence correlation spectroscopy Methods 0.000 description 5
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- 230000005653 Brownian motion process Effects 0.000 description 1
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- CPBQJMYROZQQJC-UHFFFAOYSA-N helium neon Chemical compound [He].[Ne] CPBQJMYROZQQJC-UHFFFAOYSA-N 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- 239000004065 semiconductor Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0064—Optical details of the image generation multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Optics & Photonics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Microscoopes, Condenser (AREA)
- Length Measuring Devices By Optical Means (AREA)
Abstract
Description
図1は、本発明に係る第1の実施の形態の測定装置の構成を示す図である。この測定装置では、照準検出用光学系と測定用光学系とが別光路として構成されている。
図8は、本発明に係る第2の実施の形態の測定装置の構成を示す図である。第2の実施の形態に係る測定装置は、照準検出用光学系の構成が第1の実施の形態と異なっている。従って、第1の実施の形態と同一の部分には同一の符号を付して、詳細の説明は省略する。
第3の実施の形態に係る測定装置は、照準用光学系の構成が集光光学系でない点が第1の実施形態と異なっている。従って、第1の実施の形態と同一の部分には同一の符号を付して、詳細の説明は省略する。
Claims (7)
- 容器に収容された試料に光源から発せられた光を照射し、前記試料から発せられる光を検出し、試料の物理的、あるいは化学的な特性を測定する測定装置において、
前記試料を測定するための測定用光学系と、
前記容器の底面の位置を検出するための位置検出用光学系と
を備えたことを特徴とする測定装置。 - 前記測定用光学系の測定対象と前記位置検出用光学系の検出対象が一致しないことを特徴とする請求項1に記載の測定装置。
- 前記容器の底面の位置と前記測定用光学系の測定位置との相対位置が変化したときに、位置検出用光学系の出力に応じて、前記容器の底面の位置と前記測定用光学系の測定位置との相対距離を所定値に制御する制御手段を備えたことを特徴とする請求項1または2に記載の測定装置。
- 前記所定値を可変とすることを特徴とする請求項3に記載の測定装置。
- 前記位置検出用光学系の光源はレーザ、あるいはLEDであることを特徴とする請求項1乃至4の内いずれか1項に記載の測定装置。
- 前記測定用光学系の光源から発する光の波長と前記位置検出用光学系の光源から発する光の波長とは異なることを特徴とする請求項1乃至4の内いずれか1項に記載の測定装置。
- 前記容器はマイクロプレートであることを特徴とする請求項1乃至4の内いずれか1項に記載の測定装置。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006514824A JP4914715B2 (ja) | 2004-06-21 | 2005-06-20 | 倒立顕微鏡システム |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004182510 | 2004-06-21 | ||
JP2004182510 | 2004-06-21 | ||
PCT/JP2005/011293 WO2005124321A1 (ja) | 2004-06-21 | 2005-06-20 | 測定装置 |
JP2006514824A JP4914715B2 (ja) | 2004-06-21 | 2005-06-20 | 倒立顕微鏡システム |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011161269A Division JP5469133B2 (ja) | 2004-06-21 | 2011-07-22 | 顕微鏡システム |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2005124321A1 true JPWO2005124321A1 (ja) | 2008-04-10 |
JP4914715B2 JP4914715B2 (ja) | 2012-04-11 |
Family
ID=35509807
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006514824A Active JP4914715B2 (ja) | 2004-06-21 | 2005-06-20 | 倒立顕微鏡システム |
JP2011161269A Expired - Fee Related JP5469133B2 (ja) | 2004-06-21 | 2011-07-22 | 顕微鏡システム |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2011161269A Expired - Fee Related JP5469133B2 (ja) | 2004-06-21 | 2011-07-22 | 顕微鏡システム |
Country Status (4)
Country | Link |
---|---|
US (1) | US7369220B2 (ja) |
EP (1) | EP1760455A4 (ja) |
JP (2) | JP4914715B2 (ja) |
WO (1) | WO2005124321A1 (ja) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4782593B2 (ja) * | 2006-03-13 | 2011-09-28 | 株式会社日立製作所 | 光検出装置 |
US8330087B2 (en) * | 2007-10-16 | 2012-12-11 | Cambridge Research & Instrumentation, Inc. | Spectral imaging system with dynamic optical correction |
JP5553374B2 (ja) * | 2008-06-24 | 2014-07-16 | 独立行政法人産業技術総合研究所 | 溶融材料の光学測定装置および光学測定方法 |
JP2011038922A (ja) * | 2009-08-12 | 2011-02-24 | Sony Corp | 光検出用チップおよび該光検出用チップを用いた光検出装置 |
KR101801355B1 (ko) * | 2011-03-25 | 2017-11-24 | 엘지전자 주식회사 | 회절 소자와 광원을 이용한 대상물의 거리 인식 장치 |
CN104297218B (zh) * | 2013-07-15 | 2016-09-14 | 中国科学院沈阳自动化研究所 | 远距离冶金液态金属成分的原位、在线检测装置及方法 |
JP6446432B2 (ja) * | 2014-03-05 | 2018-12-26 | 株式会社日立ハイテクノロジーズ | 顕微分光装置 |
US10473906B2 (en) | 2014-05-30 | 2019-11-12 | Nikon Corporation | Microscope |
JP6212466B2 (ja) * | 2014-11-05 | 2017-10-11 | 株式会社小野測器 | レーザ測定装置及び照準光合成装置 |
GB2534402A (en) * | 2015-01-22 | 2016-07-27 | Idea Biomedical Ltd | Auto-focussing method and device |
DE102015112628A1 (de) | 2015-07-31 | 2017-02-02 | Carl Zeiss Microscopy Gmbh | Verfahren zur Erzeugung eines digitalen Fluoreszenzbildes |
DE102016013236B4 (de) * | 2016-11-07 | 2020-07-16 | Particle Metrix Gmbh | Vorrichtung und Verfahren zum Messen der Konzentration, der Größe und des Zetapotentials von Nanopartikeln in Flüssigkeiten im Streulichtmodus und im Fluoreszenzmodus |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07109452B2 (ja) * | 1986-05-17 | 1995-11-22 | 住友電気工業株式会社 | 焦点位置検出機能付画像処理装置 |
JPH0259963A (ja) | 1988-08-26 | 1990-02-28 | Nec Corp | 文章データベース処理方式 |
JPH0580246A (ja) * | 1991-09-21 | 1993-04-02 | Canon Inc | 自動合焦装置及びそれを備えた観察装置 |
JP3517241B2 (ja) | 1993-01-18 | 2004-04-12 | エボテック バイオシステムズ アクチェン ゲゼルシャフト | 生体高分子の適応度を評価するための方法および装置 |
JPH1047918A (ja) * | 1996-08-07 | 1998-02-20 | Nikon Corp | 変位計測装置及び自動合焦機能付き顕微鏡 |
JP3061758B2 (ja) * | 1996-11-11 | 2000-07-10 | 株式会社ミツトヨ | 顕微鏡用の自動合焦装置 |
US6071748A (en) * | 1997-07-16 | 2000-06-06 | Ljl Biosystems, Inc. | Light detection device |
ATE290229T1 (de) * | 1998-12-21 | 2005-03-15 | Evotec Ag | Positionierung des messvolumens in einem scanning-mikroskopischen verfahren |
US6130745A (en) * | 1999-01-07 | 2000-10-10 | Biometric Imaging, Inc. | Optical autofocus for use with microtiter plates |
DE19916749B4 (de) * | 1999-04-14 | 2004-02-12 | Carl Zeiss Jena Gmbh | Verfahren zur Untersuchung von Proben |
US6974938B1 (en) * | 2000-03-08 | 2005-12-13 | Tibotec Bvba | Microscope having a stable autofocusing apparatus |
JP4622052B2 (ja) * | 2000-06-29 | 2011-02-02 | 株式会社ニコン | 光測定方法及びマイクロプレート |
DE10061336A1 (de) * | 2000-12-08 | 2002-06-13 | Roche Diagnostics Gmbh | System zur Analyse von Probeflüssigkeiten beinhaltend eine Lagekontrolleinheit |
JP2003315666A (ja) * | 2002-04-25 | 2003-11-06 | Seiko Instruments Inc | 投影光学装置における合焦機能 |
JP4149319B2 (ja) * | 2003-06-27 | 2008-09-10 | 富士フイルム株式会社 | 測定装置 |
JP2005098747A (ja) * | 2003-09-22 | 2005-04-14 | Fuji Photo Film Co Ltd | 測定装置 |
-
2005
- 2005-06-20 JP JP2006514824A patent/JP4914715B2/ja active Active
- 2005-06-20 EP EP05750918.4A patent/EP1760455A4/en not_active Ceased
- 2005-06-20 WO PCT/JP2005/011293 patent/WO2005124321A1/ja not_active Application Discontinuation
-
2006
- 2006-12-21 US US11/643,616 patent/US7369220B2/en active Active
-
2011
- 2011-07-22 JP JP2011161269A patent/JP5469133B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JP5469133B2 (ja) | 2014-04-09 |
US7369220B2 (en) | 2008-05-06 |
EP1760455A1 (en) | 2007-03-07 |
EP1760455A4 (en) | 2016-12-21 |
WO2005124321A1 (ja) | 2005-12-29 |
JP2011203281A (ja) | 2011-10-13 |
JP4914715B2 (ja) | 2012-04-11 |
US20070103687A1 (en) | 2007-05-10 |
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