JPS6332481A - ベンゾイルギ酸還元酵素ａの増収方法 - Google Patents
ベンゾイルギ酸還元酵素ａの増収方法Info
- Publication number
- JPS6332481A JPS6332481A JP61177347A JP17734786A JPS6332481A JP S6332481 A JPS6332481 A JP S6332481A JP 61177347 A JP61177347 A JP 61177347A JP 17734786 A JP17734786 A JP 17734786A JP S6332481 A JPS6332481 A JP S6332481A
- Authority
- JP
- Japan
- Prior art keywords
- reducing
- enzyme
- medium
- benzoylformic acid
- enzyme capable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 30
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims description 11
- VTESCYNPUGSWKG-UHFFFAOYSA-N (4-tert-butylphenyl)hydrazine;hydrochloride Chemical compound [Cl-].CC(C)(C)C1=CC=C(N[NH3+])C=C1 VTESCYNPUGSWKG-UHFFFAOYSA-N 0.000 title abstract description 6
- 241000194017 Streptococcus Species 0.000 claims abstract description 9
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims abstract description 7
- 238000012258 culturing Methods 0.000 claims abstract description 7
- 230000000813 microbial effect Effects 0.000 claims abstract description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 5
- 239000001888 Peptone Substances 0.000 claims abstract description 4
- 108010080698 Peptones Proteins 0.000 claims abstract description 4
- 235000019319 peptone Nutrition 0.000 claims abstract description 4
- FAQJJMHZNSSFSM-UHFFFAOYSA-N phenylglyoxylic acid Chemical compound OC(=O)C(=O)C1=CC=CC=C1 FAQJJMHZNSSFSM-UHFFFAOYSA-N 0.000 claims description 11
- 244000005700 microbiome Species 0.000 claims description 7
- 240000003768 Solanum lycopersicum Species 0.000 claims description 6
- 229910001429 cobalt ion Inorganic materials 0.000 claims description 2
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 claims description 2
- 235000015193 tomato juice Nutrition 0.000 abstract description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 5
- 239000008103 glucose Substances 0.000 abstract description 5
- 241000194032 Enterococcus faecalis Species 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 3
- 239000002184 metal Substances 0.000 abstract description 2
- 229910052751 metal Inorganic materials 0.000 abstract description 2
- 241000227653 Lycopersicon Species 0.000 abstract 1
- 238000009210 therapy by ultrasound Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 21
- 230000000694 effects Effects 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- IWYDHOAUDWTVEP-UHFFFAOYSA-N R-2-phenyl-2-hydroxyacetic acid Natural products OC(=O)C(O)C1=CC=CC=C1 IWYDHOAUDWTVEP-UHFFFAOYSA-N 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 4
- QBYIENPQHBMVBV-HFEGYEGKSA-N (2R)-2-hydroxy-2-phenylacetic acid Chemical compound O[C@@H](C(O)=O)c1ccccc1.O[C@@H](C(O)=O)c1ccccc1 QBYIENPQHBMVBV-HFEGYEGKSA-N 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 229940044175 cobalt sulfate Drugs 0.000 description 3
- 229910000361 cobalt sulfate Inorganic materials 0.000 description 3
- KTVIXTQDYHMGHF-UHFFFAOYSA-L cobalt(2+) sulfate Chemical compound [Co+2].[O-]S([O-])(=O)=O KTVIXTQDYHMGHF-UHFFFAOYSA-L 0.000 description 3
- 229960002510 mandelic acid Drugs 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000012137 tryptone Substances 0.000 description 3
- KWGRBVOPPLSCSI-WPRPVWTQSA-N (-)-ephedrine Chemical compound CN[C@@H](C)[C@H](O)C1=CC=CC=C1 KWGRBVOPPLSCSI-WPRPVWTQSA-N 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- IWYDHOAUDWTVEP-SSDOTTSWSA-N (R)-mandelic acid Chemical compound OC(=O)[C@H](O)C1=CC=CC=C1 IWYDHOAUDWTVEP-SSDOTTSWSA-N 0.000 description 1
- 208000035404 Autolysis Diseases 0.000 description 1
- 108010023063 Bacto-peptone Proteins 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- 229930186147 Cephalosporin Natural products 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 208000034809 Product contamination Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229940124587 cephalosporin Drugs 0.000 description 1
- 150000001780 cephalosporins Chemical class 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- MEYVLGVRTYSQHI-UHFFFAOYSA-L cobalt(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Co+2].[O-]S([O-])(=O)=O MEYVLGVRTYSQHI-UHFFFAOYSA-L 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- KWGRBVOPPLSCSI-UHFFFAOYSA-N d-ephedrine Natural products CNC(C)C(O)C1=CC=CC=C1 KWGRBVOPPLSCSI-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 229960002179 ephedrine Drugs 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001640 fractional crystallisation Methods 0.000 description 1
- 150000004688 heptahydrates Chemical class 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000028043 self proteolysis Effects 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61177347A JPS6332481A (ja) | 1986-07-28 | 1986-07-28 | ベンゾイルギ酸還元酵素ａの増収方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61177347A JPS6332481A (ja) | 1986-07-28 | 1986-07-28 | ベンゾイルギ酸還元酵素ａの増収方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6332481A true JPS6332481A (ja) | 1988-02-12 |
| JPH032516B2 JPH032516B2 (enrdf_load_stackoverflow) | 1991-01-16 |
Family
ID=16029379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61177347A Granted JPS6332481A (ja) | 1986-07-28 | 1986-07-28 | ベンゾイルギ酸還元酵素ａの増収方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6332481A (enrdf_load_stackoverflow) |
-
1986
- 1986-07-28 JP JP61177347A patent/JPS6332481A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH032516B2 (enrdf_load_stackoverflow) | 1991-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPH06505146A (ja) | ヒアルロン酸の製造 | |
| JPH04135487A (ja) | グルコースデヒドロゲナーゼおよびその製造法 | |
| EP0188712A1 (de) | Verfahren zur Gewinnung von Phenylalanin-Dehydrogenase enthaltenden Mikroorganismen und deren Verwendung zur Herstellung von L-alpha-Aminosäuren | |
| JPS6332481A (ja) | ベンゾイルギ酸還元酵素ａの増収方法 | |
| JPH04504665A (ja) | 酵母からの硝酸レダクターゼ、その調製および使用 | |
| US3669843A (en) | Process for the production of uricase | |
| JP3957132B2 (ja) | 低濁性醤油乳酸菌の分離用培地、同培地を用いる低濁性醤油乳酸菌の分離法及び同乳酸菌を用いる清澄度の高い醤油の製造法 | |
| JPS6279777A (ja) | ス−パ−オキシドデイスムタ−ゼの製造方法 | |
| JPH02268679A (ja) | １，５―アンヒドログルシトールデヒドロゲナーゼの製造法 | |
| JPH0889230A (ja) | 清酒の製造法 | |
| JPH03297358A (ja) | ビタミンｋ含量の低い納豆の製造方法 | |
| JPH01291793A (ja) | キチナーゼの製造方法 | |
| JP2933842B2 (ja) | デキストランの製造方法 | |
| JP7199459B2 (ja) | 老化、フリーラジカルによるダメージを防止する卵殻膜発酵液及びその製造方法 | |
| CN111073830B (zh) | 一株高产γ-谷氨酰转肽酶的干酪乳杆菌及其在L-茶氨酸生产中的应用 | |
| JPS63196298A (ja) | 新規微生物 | |
| JPS62208277A (ja) | イヌリナ−ゼの製造法 | |
| CN117821336A (zh) | 一种培养物、酶制剂及其制备方法和应用 | |
| JPH0339092A (ja) | 微生物が生産する物質の製造法 | |
| JPH0761256B2 (ja) | 新規麹菌及びその使用方法 | |
| JP2713720B2 (ja) | 酸性ウレアーゼの製造法 | |
| JPS6359881A (ja) | 生澱粉分解酵素生産菌 | |
| JPH06225755A (ja) | デキストランスクラーゼ生産性新規微生物及びこの新規微生物を用いるデキストランスクラーゼの生産方法 | |
| JPH02286083A (ja) | β―グルコシダーゼ及びその製造法 | |
| JPS58198291A (ja) | β−ガラクトシダ−ゼおよびその製造法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |