JPS592700A - 総ポリアミンの測定方法 - Google Patents
総ポリアミンの測定方法Info
- Publication number
- JPS592700A JPS592700A JP11232582A JP11232582A JPS592700A JP S592700 A JPS592700 A JP S592700A JP 11232582 A JP11232582 A JP 11232582A JP 11232582 A JP11232582 A JP 11232582A JP S592700 A JPS592700 A JP S592700A
- Authority
- JP
- Japan
- Prior art keywords
- polyamines
- sample
- free
- ascorbic acid
- polyamine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920000768 polyamine Polymers 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims description 28
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 26
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 19
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 17
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 17
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 17
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 239000000126 substance Substances 0.000 claims abstract description 14
- 230000000694 effects Effects 0.000 claims abstract description 9
- 108090000854 Oxidoreductases Proteins 0.000 claims abstract description 8
- 102000004316 Oxidoreductases Human genes 0.000 claims abstract description 8
- 230000001590 oxidative effect Effects 0.000 claims abstract description 5
- 102100037209 Peroxisomal N(1)-acetyl-spermine/spermidine oxidase Human genes 0.000 abstract description 23
- 108010089000 polyamine oxidase Proteins 0.000 abstract description 23
- 239000000243 solution Substances 0.000 abstract description 13
- QRMZSPFSDQBLIX-UHFFFAOYSA-N homovanillic acid Chemical compound COC1=CC(CC(O)=O)=CC=C1O QRMZSPFSDQBLIX-UHFFFAOYSA-N 0.000 abstract description 9
- 230000021615 conjugation Effects 0.000 abstract 3
- 239000007853 buffer solution Substances 0.000 abstract 2
- 239000000523 sample Substances 0.000 description 21
- 210000002700 urine Anatomy 0.000 description 19
- 238000006243 chemical reaction Methods 0.000 description 13
- 238000012360 testing method Methods 0.000 description 13
- 206010028980 Neoplasm Diseases 0.000 description 12
- 201000011510 cancer Diseases 0.000 description 11
- 210000003743 erythrocyte Anatomy 0.000 description 10
- 238000002835 absorbance Methods 0.000 description 9
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 210000002966 serum Anatomy 0.000 description 9
- 210000001124 body fluid Anatomy 0.000 description 8
- 239000010839 body fluid Substances 0.000 description 8
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 8
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 7
- 102000003992 Peroxidases Human genes 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 6
- 108040007629 peroxidase activity proteins Proteins 0.000 description 6
- 230000035484 reaction time Effects 0.000 description 6
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 description 6
- 238000005259 measurement Methods 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 239000012086 standard solution Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 238000005341 cation exchange Methods 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 4
- 239000008213 purified water Substances 0.000 description 4
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 229940063673 spermidine Drugs 0.000 description 4
- 108010024957 Ascorbate Oxidase Proteins 0.000 description 3
- -1 aliphatic basic compounds Chemical class 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 229940063675 spermine Drugs 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 206010003445 Ascites Diseases 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- JLTDJTHDQAWBAV-UHFFFAOYSA-N N,N-dimethylaniline Chemical compound CN(C)C1=CC=CC=C1 JLTDJTHDQAWBAV-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000005700 Putrescine Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 238000004393 prognosis Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 1
- NUIURNJTPRWVAP-UHFFFAOYSA-N 3,3'-Dimethylbenzidine Chemical compound C1=C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 NUIURNJTPRWVAP-UHFFFAOYSA-N 0.000 description 1
- ZTQGWROHRVYSPW-UHFFFAOYSA-N 3-(n-ethyl-3-methylanilino)-2-hydroxypropane-1-sulfonic acid Chemical compound OS(=O)(=O)CC(O)CN(CC)C1=CC=CC(C)=C1 ZTQGWROHRVYSPW-UHFFFAOYSA-N 0.000 description 1
- MLQLFEBDEPZXFY-UHFFFAOYSA-M 5-chloro-2-methoxybenzenediazonium;chloride Chemical compound [Cl-].COC1=CC=C(Cl)C=C1[N+]#N MLQLFEBDEPZXFY-UHFFFAOYSA-M 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- SBJKKFFYIZUCET-JLAZNSOCSA-N Dehydro-L-ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(=O)C1=O SBJKKFFYIZUCET-JLAZNSOCSA-N 0.000 description 1
- SBJKKFFYIZUCET-UHFFFAOYSA-N Dehydroascorbic acid Natural products OCC(O)C1OC(=O)C(=O)C1=O SBJKKFFYIZUCET-UHFFFAOYSA-N 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000219823 Medicago Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000006957 Michael reaction Methods 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 208000002151 Pleural effusion Diseases 0.000 description 1
- 101710091053 Polyamine oxidase 5 Proteins 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- DZGWFCGJZKJUFP-UHFFFAOYSA-N Tyramine Natural products NCCC1=CC=C(O)C=C1 DZGWFCGJZKJUFP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- PRQROPMIIGLWRP-BZSNNMDCSA-N chemotactic peptide Chemical compound CSCC[C@H](NC=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 PRQROPMIIGLWRP-BZSNNMDCSA-N 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 125000001295 dansyl group Chemical class [H]C1=C([H])C(N(C([H])([H])[H])C([H])([H])[H])=C2C([H])=C([H])C([H])=C(C2=C1[H])S(*)(=O)=O 0.000 description 1
- 235000020960 dehydroascorbic acid Nutrition 0.000 description 1
- 239000011615 dehydroascorbic acid Substances 0.000 description 1
- 230000003544 deproteinization Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- MEFBJEMVZONFCJ-UHFFFAOYSA-N molybdate Chemical compound [O-][Mo]([O-])(=O)=O MEFBJEMVZONFCJ-UHFFFAOYSA-N 0.000 description 1
- LCNBIHVSOPXFMR-UHFFFAOYSA-N n'-(3-aminopropyl)butane-1,4-diamine;hydron;trichloride Chemical compound Cl.Cl.Cl.NCCCCNCCCN LCNBIHVSOPXFMR-UHFFFAOYSA-N 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000006179 pH buffering agent Substances 0.000 description 1
- 229920005575 poly(amic acid) Polymers 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000002683 reaction inhibitor Substances 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000009283 thermal hydrolysis Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 229960003732 tyramine Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11232582A JPS592700A (ja) | 1982-06-28 | 1982-06-28 | 総ポリアミンの測定方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11232582A JPS592700A (ja) | 1982-06-28 | 1982-06-28 | 総ポリアミンの測定方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS592700A true JPS592700A (ja) | 1984-01-09 |
| JPS62679B2 JPS62679B2 (enrdf_load_stackoverflow) | 1987-01-08 |
Family
ID=14583845
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11232582A Granted JPS592700A (ja) | 1982-06-28 | 1982-06-28 | 総ポリアミンの測定方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS592700A (enrdf_load_stackoverflow) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012006954A (ja) * | 2011-08-09 | 2012-01-12 | Toyobo Co Ltd | 植物からのポリアミン組成物の調製方法 |
| JP2013179924A (ja) * | 2012-03-05 | 2013-09-12 | Nipro Corp | 生体試料中の馬尿酸およびメチル馬尿酸の総濃度を測定する方法 |
| JP2013179923A (ja) * | 2012-03-05 | 2013-09-12 | Nipro Corp | 生体試料中の馬尿酸およびメチル馬尿酸のそれぞれの濃度を測定する方法 |
| JP2013179925A (ja) * | 2012-03-05 | 2013-09-12 | Nipro Corp | 生体試料中の馬尿酸の濃度を測定する方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5636918A (en) * | 1979-08-31 | 1981-04-10 | Matsushita Electric Works Ltd | Carpet for foot warmer |
| JPS5639198A (en) * | 1979-09-06 | 1981-04-14 | Kazue Tanaka | Inverse pressure unit in piston filtering unit |
-
1982
- 1982-06-28 JP JP11232582A patent/JPS592700A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5636918A (en) * | 1979-08-31 | 1981-04-10 | Matsushita Electric Works Ltd | Carpet for foot warmer |
| JPS5639198A (en) * | 1979-09-06 | 1981-04-14 | Kazue Tanaka | Inverse pressure unit in piston filtering unit |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012006954A (ja) * | 2011-08-09 | 2012-01-12 | Toyobo Co Ltd | 植物からのポリアミン組成物の調製方法 |
| JP2013179924A (ja) * | 2012-03-05 | 2013-09-12 | Nipro Corp | 生体試料中の馬尿酸およびメチル馬尿酸の総濃度を測定する方法 |
| JP2013179923A (ja) * | 2012-03-05 | 2013-09-12 | Nipro Corp | 生体試料中の馬尿酸およびメチル馬尿酸のそれぞれの濃度を測定する方法 |
| JP2013179925A (ja) * | 2012-03-05 | 2013-09-12 | Nipro Corp | 生体試料中の馬尿酸の濃度を測定する方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62679B2 (enrdf_load_stackoverflow) | 1987-01-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2319937B1 (en) | Blood component measurement method utilizing hemolyzed whole blood, and kit for the method | |
| EP0033462A1 (en) | Aminopyrine improved Trinder's reagent and dosing process for hydrogen peroxide from enzimatic oxidation of metabolic substrata with the same | |
| Mizukami et al. | Electrochemical enzyme-based blood ATP and lactate sensor for a rapid and straightforward evaluation of illness severity | |
| CN112014389A (zh) | 一种抗坏血酸干扰的尿潜血检测试纸及其制备方法 | |
| JPS59125899A (ja) | 酵素法による直接ビリルビン測定用試薬および測定法 | |
| CN113655006B (zh) | 泌尿系结石成石危险因素检测试系统 | |
| JPS592700A (ja) | 総ポリアミンの測定方法 | |
| US7198905B2 (en) | Method of screening methods prediabetic state and screening reagent | |
| CN108007922B (zh) | 一种采用酶化学发光法检测葡萄糖的试剂盒 | |
| Lous et al. | A comparison of three methods, utilizing different principles, for the determination of uric acid in biological fluids | |
| JPH0698031B2 (ja) | Ｌｄ−１アイソザイムの測定法および試薬 | |
| RU2114435C1 (ru) | Способ определения нитратвосстанавливающей способности биологической жидкости | |
| Kusu et al. | Fluorometric determination of pseudocholinesterase activity in postmortem blood samples | |
| JPS63291595A (ja) | 生体試料中のグルコ−スの測定方法 | |
| JPH01174400A (ja) | ポリアミンの測定法 | |
| JPS5876100A (ja) | ラツカ−ゼの使用法 | |
| JP4073527B2 (ja) | アイソザイム活性測定法 | |
| CN119162286A (zh) | 一种抗肝素干扰的尿酸检测试剂盒及其制备方法和应用 | |
| JP3653575B2 (ja) | 血中のｄ−アラビニトールの直接比色定量法 | |
| Tanishima et al. | Automated determination of red cell methaemoglobin reductase activity by a continuous-flow system for screening hereditary methaemoglobinaemia. | |
| JPH01320998A (ja) | グルコースを含有する試料中の１，５−アンヒドログルシトールの測定法 | |
| JPS60251899A (ja) | 総ポリアミンの定量方法 | |
| Deeg et al. | A new approach to photometry of glycated hemoglobin in human blood. | |
| JP3287879B2 (ja) | １，５−アンヒドログルシトールの定量法 | |
| JPS63123400A (ja) | ３−オキソ−５β−ステロイドの定量法及びその定量用試薬 |