JPS58994A - 新規Ｎ−アセチル−β−Ｄ−グルコサミン誘導体およびこれを基質として用いるＮ−アセチル−β−Ｄ−グルコサミニダ−ゼ活性測定法 - Google Patents

新規Ｎ−アセチル−β−Ｄ−グルコサミン誘導体およびこれを基質として用いるＮ−アセチル−β−Ｄ−グルコサミニダ−ゼ活性測定法

Info

Publication number
JPS58994A
JPS58994A JP56039104A JP3910481A JPS58994A JP S58994 A JPS58994 A JP S58994A JP 56039104 A JP56039104 A JP 56039104A JP 3910481 A JP3910481 A JP 3910481A JP S58994 A JPS58994 A JP S58994A
Authority
JP
Japan
Prior art keywords
acetyl
solution
reagent
substrate
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP56039104A
Other languages
English (en)
Japanese (ja)
Other versions
JPS637196B2 (https=
Inventor
Yasunao Ogawa
小川　保直
Akira Noto
野戸　章
Yukio Mori
幸雄 森
Yoshitsuru Yoshioka
吉岡　美鶴
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shionogi and Co Ltd
Original Assignee
Shionogi and Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shionogi and Co Ltd filed Critical Shionogi and Co Ltd
Priority to JP56039104A priority Critical patent/JPS58994A/ja
Priority to US06/357,317 priority patent/US4433139A/en
Priority to DE8282400485T priority patent/DE3260690D1/de
Priority to DK115282A priority patent/DK163516C/da
Priority to EP82400485A priority patent/EP0060793B1/en
Priority to KR8201133A priority patent/KR870001924B1/ko
Priority to GB8207797A priority patent/GB2095666B/en
Publication of JPS58994A publication Critical patent/JPS58994A/ja
Priority to US06/487,703 priority patent/US4552841A/en
Publication of JPS637196B2 publication Critical patent/JPS637196B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/203—Monocyclic carbocyclic rings other than cyclohexane rings; Bicyclic carbocyclic ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/40—Triphenylmethane dye chromogens, e.g. fluorescein derivatives
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP56039104A 1981-03-17 1981-03-17 新規Ｎ−アセチル−β−Ｄ−グルコサミン誘導体およびこれを基質として用いるＮ−アセチル−β−Ｄ−グルコサミニダ−ゼ活性測定法 Granted JPS58994A (ja)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP56039104A JPS58994A (ja) 1981-03-17 1981-03-17 新規Ｎ−アセチル−β−Ｄ−グルコサミン誘導体およびこれを基質として用いるＮ−アセチル−β−Ｄ−グルコサミニダ−ゼ活性測定法
US06/357,317 US4433139A (en) 1981-03-17 1982-03-11 N-Acetyl-β-D-glucosamnides for determining N-acetyl-β-D-glucosaminidase activity
DE8282400485T DE3260690D1 (de) 1981-03-17 1982-03-16 N-acetyl-beta-d-glucosaminides for determining n-acetyl-beta-d-glucosaminidase activity
DK115282A DK163516C (da) 1981-03-17 1982-03-16 N-acetyl-beta-d-glucosaminider, deres anvendelse til bestemmelse af n-acetyl-beta-d-glucosaminidaseaktivitet og et diagnostisk udstyr, som kan benyttes hertil
EP82400485A EP0060793B1 (en) 1981-03-17 1982-03-16 N-acetyl-beta-d-glucosaminides for determining n-acetyl-beta-d-glucosaminidase activity
KR8201133A KR870001924B1 (ko) 1981-03-17 1982-03-17 N-아세틸- β-D-글루코사미니다아제 활성 측정을 위한 N-아세틸-β -D-글루코사미니드류의 제조방법
GB8207797A GB2095666B (en) 1981-03-17 1982-03-17 N-acetyl- -d-glucosaminides
US06/487,703 US4552841A (en) 1981-03-17 1983-04-21 N-Acetyl-β-D-glucosaminides for determining N-acetyl-β-D-glucosaminidase activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56039104A JPS58994A (ja) 1981-03-17 1981-03-17 新規Ｎ−アセチル−β−Ｄ−グルコサミン誘導体およびこれを基質として用いるＮ−アセチル−β−Ｄ−グルコサミニダ−ゼ活性測定法

Publications (2)

Publication Number Publication Date
JPS58994A true JPS58994A (ja) 1983-01-06
JPS637196B2 JPS637196B2 (https=) 1988-02-15

Family

ID=12543756

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56039104A Granted JPS58994A (ja) 1981-03-17 1981-03-17 新規Ｎ−アセチル−β−Ｄ−グルコサミン誘導体およびこれを基質として用いるＮ−アセチル−β−Ｄ−グルコサミニダ−ゼ活性測定法

Country Status (7)

Country Link
US (1) US4433139A (https=)
EP (1) EP0060793B1 (https=)
JP (1) JPS58994A (https=)
KR (1) KR870001924B1 (https=)
DE (1) DE3260690D1 (https=)
DK (1) DK163516C (https=)
GB (1) GB2095666B (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4552841A (en) * 1981-03-17 1985-11-12 Shionogi & Co., Ltd. N-Acetyl-β-D-glucosaminides for determining N-acetyl-β-D-glucosaminidase activity
DE3345748A1 (de) * 1983-12-17 1985-08-29 Boehringer Mannheim Gmbh, 6800 Mannheim Phenolsulfonphthaleinyl-ss-d-galactoside, verfahren zu deren herstellung sowie deren verwendung zur bestimmung der ss-d-galactosidase
US5034317A (en) * 1987-01-30 1991-07-23 Polaroid Corporation Enzyme controlled release system and organic conjugate reactant
IL85581A (en) * 1987-05-21 1992-05-25 Technicon Instr Substrate for beta-galactosidase comprising derivatives of 4-nitrophenyl-beta-d-galactopyranoside and beta-galactosidase immunoassay containing said substrate
JPH0650991B2 (ja) * 1987-06-11 1994-07-06 塩野義製薬株式会社 ＮＡＧａｓｅ活性測定用試薬および測定方法
JP2722874B2 (ja) * 1991-08-01 1998-03-09 日東紡績株式会社 新規Ｎ−アセチル−β−Ｄ−グルコサミン誘導体及びこれを基質に用いたＮ−アセチル−β−Ｄ−グルコサミニダーゼ活性測定法
US5208326A (en) * 1991-11-08 1993-05-04 Miles Inc. 8-hydroxy-2h-dibenz(b,f)azepin-2-one dyes
CN111850092A (zh) * 2020-07-01 2020-10-30 甘肃省地质矿产勘查开发局第三地质矿产勘查院(甘肃遥感地质中心、甘肃地质灾害防治工程勘查设计院、甘肃省地质遗迹评估中心、甘肃省中心实验室) 基于土壤酶活性测定的土壤生物学活性和生产力评价方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2453069A1 (de) * 1974-11-06 1976-05-13 Schering Ag Neue glykoside
US4152513A (en) * 1977-06-01 1979-05-01 University Of Delaware Preparation of alkyl glycosides of amino sugars

Also Published As

Publication number Publication date
DK115282A (da) 1982-09-18
DK163516C (da) 1992-07-27
EP0060793B1 (en) 1984-09-12
DE3260690D1 (de) 1984-10-18
KR830009136A (ko) 1983-12-17
DK163516B (da) 1992-03-09
GB2095666A (en) 1982-10-06
KR870001924B1 (ko) 1987-10-22
EP0060793A1 (en) 1982-09-22
JPS637196B2 (https=) 1988-02-15
US4433139A (en) 1984-02-21
GB2095666B (en) 1985-02-27

Similar Documents

Publication Publication Date Title
EP0231125B1 (en) Hydrolyzable fluorescent substrates and analytical determinations using same
US4716222A (en) Substrates for hydrolases
FI61916B (fi) Foerfarande och reagens foer bestaemning av alfa-amylas
FI81359B (fi) Glykosider av resorufin-derivat, foerfarande foer framstaellning daerav samt deras anvaendning foer bestaemning av aktiviteten av glykosidaser.
US4668622A (en) Phenolsulphonphthaleinyl-β-D-galactosides and diagnostic agents containing them
JPH03151397A (ja) 加水分解酵素基質、加水分解活性を有する物質の検出剤及び検出法並びに分析物を検出するための試験条片
JPS58994A (ja) 新規Ｎ−アセチル−β−Ｄ−グルコサミン誘導体およびこれを基質として用いるＮ−アセチル−β−Ｄ−グルコサミニダ−ゼ活性測定法
US5077200A (en) Azo dye chromogenic substrates
JPH04305593A (ja) Ｎ−およびｏ−置換アミノフェノール誘導体、その製造用中間体およびその使用
EP0153872A2 (en) Method for the determination of the reduced form of nicotinamide adenine dinucleotide
US5399488A (en) Method for assaying enzyme activity using N-acetyl-β-D-glucosamine derivatives
US4552841A (en) N-Acetyl-β-D-glucosaminides for determining N-acetyl-β-D-glucosaminidase activity
US5272260A (en) Reagents and methods for the determination of glycohydrolytic enzymes
US5202233A (en) Process for the detection of substances with hydrolase activity
JP3994461B2 (ja) ヒドロキシアルキルピリジン誘導体
US5126329A (en) Glucosamine derivatives and compositions reagents and containing the same
US5260428A (en) Agent for the detection of substances with hydrolase activity
JPH0662569B2 (ja) 色原体アクリジノン酵素基質及びその製造方法
EP0292169B1 (en) Substrates for B-galactosidase
JPH0730107B2 (ja) 加水分解酵素活性を有する物質の検出方法及び二環式化合物
JPH01160990A (ja) １−ナフトールフタレインモノホスフエート、その製法、アルカリホスフアターゼの測定法及びアルカリホスフアターゼ検出用診断剤
JPH0358996A (ja) インドフェニル―β―マルトオリゴシド誘導体、このものを有効成分とするα―アミラーゼ活性測定用試薬及びこれを用いたα―アミラーゼ活性の測定方法
CS273184B2 (en) Method of resorufin's derivative glycoside production
JPWO2000055167A1 (ja) ２−ピリジンチオール誘導体及びそれを含有するｎａｇ活性測定用試薬