JPS5592697A - Method for quantitative analysis using immobilized enzyme - Google Patents
Method for quantitative analysis using immobilized enzymeInfo
- Publication number
- JPS5592697A JPS5592697A JP16516978A JP16516978A JPS5592697A JP S5592697 A JPS5592697 A JP S5592697A JP 16516978 A JP16516978 A JP 16516978A JP 16516978 A JP16516978 A JP 16516978A JP S5592697 A JPS5592697 A JP S5592697A
- Authority
- JP
- Japan
- Prior art keywords
- solution
- oxidizing
- hydrogen peroxide
- agent
- immobilized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: To determine the amount to hydrogen peroxide in a solution, in high accuracy, by oxidizing a detecting agent added to the solution with the hydrogen peroxide in the presence of an immobilized peroxidase as a catalyst, and determining the oxidized detecting agent.
CONSTITUTION: Hydrogen peroxide in a solution is determined by (1) oxidizing an intermediate electron transfer material such as metal ions having two or more ionic valences, with the hydrogen peroxide in the presence of an immobilized peroxidase as a catalyst, (2) separating the immobilized enzyme from the solution containing the oxidized intermediate electron transfer material, (3) adding a detecting agnet to the solution thereby oxidizing the agent, and (4) determining the amount of the oxidized detecting agent in the solution. A compound such as saccharides, purine derivatives, etc. can also be determined by (1) oxidizing the compound in the presence of an immobilized oxidase, and (2) carrying out the above procedures using the produced hydrogen peroxide as an oxidizing agent.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16516978A JPS5592697A (en) | 1978-12-29 | 1978-12-29 | Method for quantitative analysis using immobilized enzyme |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP16516978A JPS5592697A (en) | 1978-12-29 | 1978-12-29 | Method for quantitative analysis using immobilized enzyme |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5592697A true JPS5592697A (en) | 1980-07-14 |
Family
ID=15807163
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP16516978A Pending JPS5592697A (en) | 1978-12-29 | 1978-12-29 | Method for quantitative analysis using immobilized enzyme |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5592697A (en) |
-
1978
- 1978-12-29 JP JP16516978A patent/JPS5592697A/en active Pending
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