JPS56151498A - Measuring method of substrate concentration or enzyme activity - Google Patents
Measuring method of substrate concentration or enzyme activityInfo
- Publication number
- JPS56151498A JPS56151498A JP5600280A JP5600280A JPS56151498A JP S56151498 A JPS56151498 A JP S56151498A JP 5600280 A JP5600280 A JP 5600280A JP 5600280 A JP5600280 A JP 5600280A JP S56151498 A JPS56151498 A JP S56151498A
- Authority
- JP
- Japan
- Prior art keywords
- ascorbic acid
- compounds
- act
- measuring method
- enzyme activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: In the titled measuring method utilizing oxidation and reduction reactions, the determination is made after the removal of inhibiting substances by making ascorbic acid oxidase act on ascorbic acid and glutathion-dehydroascorbic acid oxidoreductase on SH compounds.
CONSTITUTION: On the determination, before or when the oxidation and reduction reaction is effected, ascorbic acid oxidase is made to act on ascorbic acid and the glutathion dehydroascorbic acid oxidoreductase is made to act on both the dehydroascorbic acid formed and SH compounds, which is converted into the oxidation type SH compounds. The amounts of enzymes added can be decided regardless of the amount of the reductive substances, but a large amount of the enzymes removes the inhibiting substance in a shorter time. Thus, the inhibiting substances, that is, ascorbic acid and SH compounds, are removed and the concentration of the substrate or enzyme activity can be determined.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5600280A JPS56151498A (en) | 1980-04-26 | 1980-04-26 | Measuring method of substrate concentration or enzyme activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5600280A JPS56151498A (en) | 1980-04-26 | 1980-04-26 | Measuring method of substrate concentration or enzyme activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS56151498A true JPS56151498A (en) | 1981-11-24 |
Family
ID=13014856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5600280A Pending JPS56151498A (en) | 1980-04-26 | 1980-04-26 | Measuring method of substrate concentration or enzyme activity |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56151498A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6315168A (en) * | 1986-06-21 | 1988-01-22 | ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | Method of measuring serum fructosamine content in blood or sample induced from blood |
| JPH02200199A (en) * | 1989-01-30 | 1990-08-08 | Sanwa Kagaku Kenkyusho Co Ltd | Determining composition of bile acid in blood |
-
1980
- 1980-04-26 JP JP5600280A patent/JPS56151498A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6315168A (en) * | 1986-06-21 | 1988-01-22 | ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | Method of measuring serum fructosamine content in blood or sample induced from blood |
| JPH02200199A (en) * | 1989-01-30 | 1990-08-08 | Sanwa Kagaku Kenkyusho Co Ltd | Determining composition of bile acid in blood |
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