JPS56127100A - Determination of component in living body fluid - Google Patents

Determination of component in living body fluid

Info

Publication number
JPS56127100A
JPS56127100A JP2921280A JP2921280A JPS56127100A JP S56127100 A JPS56127100 A JP S56127100A JP 2921280 A JP2921280 A JP 2921280A JP 2921280 A JP2921280 A JP 2921280A JP S56127100 A JPS56127100 A JP S56127100A
Authority
JP
Japan
Prior art keywords
determination
component
aminoantipyrine
peroxidase
coupler
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2921280A
Other languages
Japanese (ja)
Other versions
JPS5729159B2 (en
Inventor
Norimasa Takizawa
Masayuki Mitsukide
Shigeru Miwa
Fumio Nouchi
Yukari Nakamura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
YATORON KK
Mitsubishi Kagaku Iatron Inc
Original Assignee
YATORON KK
Mitsubishi Kagaku Iatron Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by YATORON KK, Mitsubishi Kagaku Iatron Inc filed Critical YATORON KK
Priority to JP2921280A priority Critical patent/JPS56127100A/en
Publication of JPS56127100A publication Critical patent/JPS56127100A/en
Publication of JPS5729159B2 publication Critical patent/JPS5729159B2/ja
Granted legal-status Critical Current

Links

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  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To eliminate the interfering substance in a specimen, by separating the substrate or enzyme which reacts with the objective component and the coupler of oxidative condensation reaction from 4-aminoantipyrine and peroxidase, and carrying out the determination by redox reaction.
CONSTITUTION: In the colorimetric determination of the objective component by the oxidative condensation 4-aminoantipyrine, a specific coupler, and hydrogen peroxide produce by redox reaction, in the presence of peroxidase, the reagent is divided into a component containing the coupler and a substrate or enzyme which reacts directly with the objective component and the second component containing 4-aminoantipyrine and peroxidase. The interfering substance in the specimen is removed by the latter reagent before determination. The interfering substance is, e.g. glycerol in the triglyceride determination, maltose and glucose in the α-amylase activity determination, etc.
COPYRIGHT: (C)1981,JPO&Japio
JP2921280A 1980-03-10 1980-03-10 Determination of component in living body fluid Granted JPS56127100A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2921280A JPS56127100A (en) 1980-03-10 1980-03-10 Determination of component in living body fluid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2921280A JPS56127100A (en) 1980-03-10 1980-03-10 Determination of component in living body fluid

Publications (2)

Publication Number Publication Date
JPS56127100A true JPS56127100A (en) 1981-10-05
JPS5729159B2 JPS5729159B2 (en) 1982-06-21

Family

ID=12269878

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2921280A Granted JPS56127100A (en) 1980-03-10 1980-03-10 Determination of component in living body fluid

Country Status (1)

Country Link
JP (1) JPS56127100A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112074609A (en) * 2018-05-10 2020-12-11 东洋纺株式会社 Method for suppressing sensitivity reduction of biological component measurement kit

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112074609A (en) * 2018-05-10 2020-12-11 东洋纺株式会社 Method for suppressing sensitivity reduction of biological component measurement kit
CN112074609B (en) * 2018-05-10 2024-02-09 东洋纺株式会社 Method for suppressing sensitivity decrease of reagent kit for measuring biological component

Also Published As

Publication number Publication date
JPS5729159B2 (en) 1982-06-21

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