JPS56142448A - Engyme electrode - Google Patents
Engyme electrodeInfo
- Publication number
- JPS56142448A JPS56142448A JP4707680A JP4707680A JPS56142448A JP S56142448 A JPS56142448 A JP S56142448A JP 4707680 A JP4707680 A JP 4707680A JP 4707680 A JP4707680 A JP 4707680A JP S56142448 A JPS56142448 A JP S56142448A
- Authority
- JP
- Japan
- Prior art keywords
- layer
- catalase
- fixing
- solution
- glucose oxidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To improve rectilinear characteristic in high-density region, by providing on a hydrogen peroxide detecting electrode an oxidoreductase fixed layer, which forms H2O2 by selectively reacting an objective substance in a specimen solution, and a catalase fixed layer on this layer. CONSTITUTION:On a hydrogen peroxide detecting electrode 3 which is prepared by coating platinum on surface of a negative glass by thermal decomposition process and providing electric connection by silver paste, glucose oxidase solution is spread and it is made to react in vapor of gluter-aldehyde for fixing of bridge, and by doing so, a glucose oxidase fixing electrode 2 is obtained. Further, a bridge fixing layer 1 is formed by spreading on this fixing layer catalase solution in which small amount of albumin is added or a solution prepared by mixture of catalase and ascorbic acid oxidase. By doing so, H2O2 formed in the glucose oxidase layer is decomposed in the catalase fixing layer to form oxygen, and therefore, rectilinear characteristic in high-density region can be improved.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4707680A JPS56142448A (en) | 1980-04-09 | 1980-04-09 | Engyme electrode |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4707680A JPS56142448A (en) | 1980-04-09 | 1980-04-09 | Engyme electrode |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS56142448A true JPS56142448A (en) | 1981-11-06 |
JPS6257942B2 JPS6257942B2 (en) | 1987-12-03 |
Family
ID=12765071
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP4707680A Granted JPS56142448A (en) | 1980-04-09 | 1980-04-09 | Engyme electrode |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS56142448A (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS56163447A (en) * | 1980-05-22 | 1981-12-16 | Matsushita Electric Ind Co Ltd | Enzyme electrode |
JPS63111454A (en) * | 1986-10-29 | 1988-05-16 | Nec Corp | Production of immobilized enzyme film |
US20120211372A1 (en) * | 2011-02-22 | 2012-08-23 | The Nitrate Elimination Co., Inc. | Systems and Methods for Enzymatic Oxygen Removal |
CN103163099A (en) * | 2013-02-22 | 2013-06-19 | 南通大学 | Antioxidant capacity measuring method based on triangle silver nanoparticle prism |
CN113106143A (en) * | 2021-04-01 | 2021-07-13 | 广州南雪医疗器械有限公司 | Test paper for detecting uric acid |
-
1980
- 1980-04-09 JP JP4707680A patent/JPS56142448A/en active Granted
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS56163447A (en) * | 1980-05-22 | 1981-12-16 | Matsushita Electric Ind Co Ltd | Enzyme electrode |
JPH0136062B2 (en) * | 1980-05-22 | 1989-07-28 | Matsushita Electric Ind Co Ltd | |
JPS63111454A (en) * | 1986-10-29 | 1988-05-16 | Nec Corp | Production of immobilized enzyme film |
US20120211372A1 (en) * | 2011-02-22 | 2012-08-23 | The Nitrate Elimination Co., Inc. | Systems and Methods for Enzymatic Oxygen Removal |
US9187779B2 (en) * | 2011-02-22 | 2015-11-17 | The Nitrate Elimination Co., Inc. | Systems and methods for enzymatic oxygen removal |
CN103163099A (en) * | 2013-02-22 | 2013-06-19 | 南通大学 | Antioxidant capacity measuring method based on triangle silver nanoparticle prism |
CN103163099B (en) * | 2013-02-22 | 2014-11-26 | 南通大学 | Antioxidant capacity measuring method based on triangle silver nanoparticle prism |
CN113106143A (en) * | 2021-04-01 | 2021-07-13 | 广州南雪医疗器械有限公司 | Test paper for detecting uric acid |
Also Published As
Publication number | Publication date |
---|---|
JPS6257942B2 (en) | 1987-12-03 |
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