JPS56142449A - Engyme electrode - Google Patents

Engyme electrode

Info

Publication number
JPS56142449A
JPS56142449A JP4708080A JP4708080A JPS56142449A JP S56142449 A JPS56142449 A JP S56142449A JP 4708080 A JP4708080 A JP 4708080A JP 4708080 A JP4708080 A JP 4708080A JP S56142449 A JPS56142449 A JP S56142449A
Authority
JP
Japan
Prior art keywords
fixing
membrane
catalase
enzyme
hydrogen peroxide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP4708080A
Other languages
Japanese (ja)
Other versions
JPS6319818B2 (en
Inventor
Shiro Nankai
Kenichi Nakamura
Takashi Iijima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Holdings Corp
Original Assignee
Matsushita Electric Industrial Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Matsushita Electric Industrial Co Ltd filed Critical Matsushita Electric Industrial Co Ltd
Priority to JP4708080A priority Critical patent/JPS56142449A/en
Publication of JPS56142449A publication Critical patent/JPS56142449A/en
Publication of JPS6319818B2 publication Critical patent/JPS6319818B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To improve response rectilinear characteristics, by fixing enzyme, which reacts an objective substrate to form H2O2, on hydrogen peroxide detecting electrode side of porous body membrane and also fixing catalase on the other side. CONSTITUTION:A hydrogen peroxide detecting electrode 4 is prepared by coating platinum on surface of a negative glass by thermal decomposition process and providing electric connection by silver paste. Polycarbonate porous membrane is used as an enzyme fixing carrier membrane 2, a mixture solution of albumin-added catalase and ascorbic acid oxidase is applied onto one side of this membrane, it is made to react in vapor of glutaraldehyde for fixing of bridge and formation of a catalase fixing layer 1, and a glucose oxidase enzyme fixing layer 3 is formed on the surface on the other side. Glucose oxidase side of thus obtained enzyme fixing membrane is tightly fixed to the hydrogen peroxide detecting electrode 4. By doing so, H2O2 is decomposed to oxygen by the catalase layer, and therefore, rectilinear characteristic in high-density region can be improved.
JP4708080A 1980-04-09 1980-04-09 Engyme electrode Granted JPS56142449A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4708080A JPS56142449A (en) 1980-04-09 1980-04-09 Engyme electrode

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4708080A JPS56142449A (en) 1980-04-09 1980-04-09 Engyme electrode

Publications (2)

Publication Number Publication Date
JPS56142449A true JPS56142449A (en) 1981-11-06
JPS6319818B2 JPS6319818B2 (en) 1988-04-25

Family

ID=12765189

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4708080A Granted JPS56142449A (en) 1980-04-09 1980-04-09 Engyme electrode

Country Status (1)

Country Link
JP (1) JPS56142449A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61128152A (en) * 1984-11-27 1986-06-16 Hitachi Ltd Biochemical sensor

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5481177A (en) * 1977-12-13 1979-06-28 Omron Tateisi Electronics Co Electrode having double fixed enzyme layers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5481177A (en) * 1977-12-13 1979-06-28 Omron Tateisi Electronics Co Electrode having double fixed enzyme layers

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61128152A (en) * 1984-11-27 1986-06-16 Hitachi Ltd Biochemical sensor

Also Published As

Publication number Publication date
JPS6319818B2 (en) 1988-04-25

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