JPS57177699A - Determination of alpha-amylase by means of enzyme electrode method - Google Patents

Determination of alpha-amylase by means of enzyme electrode method

Info

Publication number
JPS57177699A
JPS57177699A JP56063456A JP6345681A JPS57177699A JP S57177699 A JPS57177699 A JP S57177699A JP 56063456 A JP56063456 A JP 56063456A JP 6345681 A JP6345681 A JP 6345681A JP S57177699 A JPS57177699 A JP S57177699A
Authority
JP
Japan
Prior art keywords
glucose
electrode
enzyme electrode
membrane
current
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP56063456A
Other languages
Japanese (ja)
Other versions
JPS634661B2 (en
Inventor
Hisao Osawa
Kenji Harada
Izumi Nakagawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
FUJI DENKI SOUGOU KENKYUSHO KK
Fuji Electric Co Ltd
Original Assignee
FUJI DENKI SOUGOU KENKYUSHO KK
Fuji Electric Co Ltd
Fuji Electric Corporate Research and Development Ltd
Fuji Electric Manufacturing Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by FUJI DENKI SOUGOU KENKYUSHO KK, Fuji Electric Co Ltd, Fuji Electric Corporate Research and Development Ltd, Fuji Electric Manufacturing Co Ltd filed Critical FUJI DENKI SOUGOU KENKYUSHO KK
Priority to JP56063456A priority Critical patent/JPS57177699A/en
Publication of JPS57177699A publication Critical patent/JPS57177699A/en
Publication of JPS634661B2 publication Critical patent/JPS634661B2/ja
Granted legal-status Critical Current

Links

Abstract

PURPOSE: Maltopentaose and α-glucositase are made to act on a prescribed amount of sample and the glucose formed is detected as a current change in the enzyme electrode with a glucose oxidase membrane, thus effecting determination rapidly simply with high selectivity.
CONSTITUTION: The enzyme electrode has the platinum electrode 10 as the anode and the silver electrode 12 as the cathode where both electrodes are insulated from each other and supported by the support 14. The surfaces of both electrodes 10, 12 are tightly sealed through a membrane 16 including immobilized glucose-oxidase with the O-ring 20. When maltopentaose and α-glucositase are made to act on a prescribed amount of sample and glucose is formed, the glucose generates hydrogen peroxide at the glucose oxidase membrane 16 and the hydrogen peroxide is oxidized at the platinum electrode 10 and reduced at the silver electrode 12 to cause a reaction current. The current is measure and converted into the glucose concentration.
COPYRIGHT: (C)1982,JPO&Japio
JP56063456A 1981-04-28 1981-04-28 Determination of alpha-amylase by means of enzyme electrode method Granted JPS57177699A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP56063456A JPS57177699A (en) 1981-04-28 1981-04-28 Determination of alpha-amylase by means of enzyme electrode method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56063456A JPS57177699A (en) 1981-04-28 1981-04-28 Determination of alpha-amylase by means of enzyme electrode method

Publications (2)

Publication Number Publication Date
JPS57177699A true JPS57177699A (en) 1982-11-01
JPS634661B2 JPS634661B2 (en) 1988-01-29

Family

ID=13229749

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56063456A Granted JPS57177699A (en) 1981-04-28 1981-04-28 Determination of alpha-amylase by means of enzyme electrode method

Country Status (1)

Country Link
JP (1) JPS57177699A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3423406A1 (en) * 1983-06-24 1985-01-10 Hitachi, Ltd., Tokio/Tokyo Method for the determination of alpha -amylase
US4547280A (en) * 1983-11-28 1985-10-15 Hitachi, Ltd. Maltose sensor
US4725539A (en) * 1983-03-08 1988-02-16 Oriental Yeast Co. Ltd. Method for analyzing plural oxidizable components in a liquid
JPH0223891A (en) * 1983-08-08 1990-01-26 Boehringer Mannheim Gmbh Measurement of alpha-amirase and reagent therefor
US5175088A (en) * 1983-03-08 1992-12-29 Oriental Yeast Co. Ltd. Rapid analysis of plural components
JP2006198684A (en) * 2005-01-17 2006-08-03 Max Co Ltd Punch

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4725539A (en) * 1983-03-08 1988-02-16 Oriental Yeast Co. Ltd. Method for analyzing plural oxidizable components in a liquid
US5175088A (en) * 1983-03-08 1992-12-29 Oriental Yeast Co. Ltd. Rapid analysis of plural components
DE3423406A1 (en) * 1983-06-24 1985-01-10 Hitachi, Ltd., Tokio/Tokyo Method for the determination of alpha -amylase
JPH0223891A (en) * 1983-08-08 1990-01-26 Boehringer Mannheim Gmbh Measurement of alpha-amirase and reagent therefor
JPH0515436B2 (en) * 1983-08-08 1993-03-01 Boehringer Mannheim Gmbh
US4547280A (en) * 1983-11-28 1985-10-15 Hitachi, Ltd. Maltose sensor
JP2006198684A (en) * 2005-01-17 2006-08-03 Max Co Ltd Punch

Also Published As

Publication number Publication date
JPS634661B2 (en) 1988-01-29

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