JPS57177699A - Determination of alpha-amylase by means of enzyme electrode method - Google Patents
Determination of alpha-amylase by means of enzyme electrode methodInfo
- Publication number
- JPS57177699A JPS57177699A JP56063456A JP6345681A JPS57177699A JP S57177699 A JPS57177699 A JP S57177699A JP 56063456 A JP56063456 A JP 56063456A JP 6345681 A JP6345681 A JP 6345681A JP S57177699 A JPS57177699 A JP S57177699A
- Authority
- JP
- Japan
- Prior art keywords
- glucose
- electrode
- enzyme electrode
- membrane
- current
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: Maltopentaose and α-glucositase are made to act on a prescribed amount of sample and the glucose formed is detected as a current change in the enzyme electrode with a glucose oxidase membrane, thus effecting determination rapidly simply with high selectivity.
CONSTITUTION: The enzyme electrode has the platinum electrode 10 as the anode and the silver electrode 12 as the cathode where both electrodes are insulated from each other and supported by the support 14. The surfaces of both electrodes 10, 12 are tightly sealed through a membrane 16 including immobilized glucose-oxidase with the O-ring 20. When maltopentaose and α-glucositase are made to act on a prescribed amount of sample and glucose is formed, the glucose generates hydrogen peroxide at the glucose oxidase membrane 16 and the hydrogen peroxide is oxidized at the platinum electrode 10 and reduced at the silver electrode 12 to cause a reaction current. The current is measure and converted into the glucose concentration.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56063456A JPS57177699A (en) | 1981-04-28 | 1981-04-28 | Determination of alpha-amylase by means of enzyme electrode method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56063456A JPS57177699A (en) | 1981-04-28 | 1981-04-28 | Determination of alpha-amylase by means of enzyme electrode method |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS57177699A true JPS57177699A (en) | 1982-11-01 |
JPS634661B2 JPS634661B2 (en) | 1988-01-29 |
Family
ID=13229749
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP56063456A Granted JPS57177699A (en) | 1981-04-28 | 1981-04-28 | Determination of alpha-amylase by means of enzyme electrode method |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57177699A (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3423406A1 (en) * | 1983-06-24 | 1985-01-10 | Hitachi, Ltd., Tokio/Tokyo | Method for the determination of alpha -amylase |
US4547280A (en) * | 1983-11-28 | 1985-10-15 | Hitachi, Ltd. | Maltose sensor |
US4725539A (en) * | 1983-03-08 | 1988-02-16 | Oriental Yeast Co. Ltd. | Method for analyzing plural oxidizable components in a liquid |
JPH0223891A (en) * | 1983-08-08 | 1990-01-26 | Boehringer Mannheim Gmbh | Measurement of alpha-amirase and reagent therefor |
US5175088A (en) * | 1983-03-08 | 1992-12-29 | Oriental Yeast Co. Ltd. | Rapid analysis of plural components |
JP2006198684A (en) * | 2005-01-17 | 2006-08-03 | Max Co Ltd | Punch |
-
1981
- 1981-04-28 JP JP56063456A patent/JPS57177699A/en active Granted
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4725539A (en) * | 1983-03-08 | 1988-02-16 | Oriental Yeast Co. Ltd. | Method for analyzing plural oxidizable components in a liquid |
US5175088A (en) * | 1983-03-08 | 1992-12-29 | Oriental Yeast Co. Ltd. | Rapid analysis of plural components |
DE3423406A1 (en) * | 1983-06-24 | 1985-01-10 | Hitachi, Ltd., Tokio/Tokyo | Method for the determination of alpha -amylase |
JPH0223891A (en) * | 1983-08-08 | 1990-01-26 | Boehringer Mannheim Gmbh | Measurement of alpha-amirase and reagent therefor |
JPH0515436B2 (en) * | 1983-08-08 | 1993-03-01 | Boehringer Mannheim Gmbh | |
US4547280A (en) * | 1983-11-28 | 1985-10-15 | Hitachi, Ltd. | Maltose sensor |
JP2006198684A (en) * | 2005-01-17 | 2006-08-03 | Max Co Ltd | Punch |
Also Published As
Publication number | Publication date |
---|---|
JPS634661B2 (en) | 1988-01-29 |
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