FR2455279A1 - Enzyme electrodes for NAD or NADP determn. - with enzyme layer contg. glucose oxidase and glucose dehydrogenase - Google Patents

Enzyme electrodes for NAD or NADP determn. - with enzyme layer contg. glucose oxidase and glucose dehydrogenase

Info

Publication number
FR2455279A1
FR2455279A1 FR8007590A FR8007590A FR2455279A1 FR 2455279 A1 FR2455279 A1 FR 2455279A1 FR 8007590 A FR8007590 A FR 8007590A FR 8007590 A FR8007590 A FR 8007590A FR 2455279 A1 FR2455279 A1 FR 2455279A1
Authority
FR
France
Prior art keywords
nad
enzyme
glucose
determn
electrodes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR8007590A
Other languages
French (fr)
Other versions
FR2455279B3 (en
Inventor
Frieder Scheller
Dorothea Pfeiffer
Ingrid Seyer
Martina Siepe
Klaus Riedel
Oda Scheller
Michael Janchen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Akademie der Wissenschaften der DDR
Original Assignee
Akademie der Wissenschaften der DDR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DD21246579A external-priority patent/DD142973B1/en
Priority claimed from DD21292179A external-priority patent/DD143180A1/en
Application filed by Akademie der Wissenschaften der DDR filed Critical Akademie der Wissenschaften der DDR
Publication of FR2455279A1 publication Critical patent/FR2455279A1/en
Application granted granted Critical
Publication of FR2455279B3 publication Critical patent/FR2455279B3/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood

Abstract

Enzyme electrodes comprise an enzyme layer deposited on an oxygen or hydrogen peroxide-indicator electrode and covered by a dialysis membrane. The enzyme layer contains glucose oxidase and glucose dehydrogenase in the form of a homogeneous mixt. or as separate sub-layers. A predetermined amt. of glucose must be added to the test soln. The electrodes can be used to determine NAD(P) concns in physiological and microbiological solns. or for continuously monitoring changes in NAD(P) concn. They can also be modified for determn. of substrates of NAD(P)H-dependent dehydrogenases.
FR8007590A 1979-04-25 1980-04-03 Enzyme electrodes for NAD or NADP determn. - with enzyme layer contg. glucose oxidase and glucose dehydrogenase Granted FR2455279A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DD21246579A DD142973B1 (en) 1979-04-25 1979-04-25 ENZYME ELECTRODE FOR ATP DETERMINATION
DD21292179A DD143180A1 (en) 1979-05-16 1979-05-16 ENZYME ELECTRODE FOR NAD (P) DETERMINATION

Publications (2)

Publication Number Publication Date
FR2455279A1 true FR2455279A1 (en) 1980-11-21
FR2455279B3 FR2455279B3 (en) 1982-01-29

Family

ID=25747652

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8007590A Granted FR2455279A1 (en) 1979-04-25 1980-04-03 Enzyme electrodes for NAD or NADP determn. - with enzyme layer contg. glucose oxidase and glucose dehydrogenase

Country Status (2)

Country Link
DE (1) DE3010119A1 (en)
FR (1) FR2455279A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0079502A1 (en) * 1981-11-05 1983-05-25 Miles Laboratories, Inc. Multilayer enzyme electrode membrane, method of making same and polarographic cell structure
EP0125136A2 (en) * 1983-05-05 1984-11-14 MediSense, Inc. Assay systems utilising more than one enzyme
EP0250068A1 (en) * 1986-04-02 1987-12-23 Unitika Ltd. Enzyme sensor

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0079502A1 (en) * 1981-11-05 1983-05-25 Miles Laboratories, Inc. Multilayer enzyme electrode membrane, method of making same and polarographic cell structure
EP0125136A2 (en) * 1983-05-05 1984-11-14 MediSense, Inc. Assay systems utilising more than one enzyme
EP0125136A3 (en) * 1983-05-05 1987-01-14 Genetics International, Inc. Assay systems utilising more than one enzyme
EP0250068A1 (en) * 1986-04-02 1987-12-23 Unitika Ltd. Enzyme sensor
US4900423A (en) * 1986-04-02 1990-02-13 Unitika Ltd. Enzyme sensor using immobilized glucokinase

Also Published As

Publication number Publication date
DE3010119A1 (en) 1980-11-06
FR2455279B3 (en) 1982-01-29

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