JPH10507349A - パッチド遺伝子及びそれらの使用 - Google Patents
パッチド遺伝子及びそれらの使用Info
- Publication number
- JPH10507349A JPH10507349A JP8512723A JP51272396A JPH10507349A JP H10507349 A JPH10507349 A JP H10507349A JP 8512723 A JP8512723 A JP 8512723A JP 51272396 A JP51272396 A JP 51272396A JP H10507349 A JPH10507349 A JP H10507349A
- Authority
- JP
- Japan
- Prior art keywords
- patched
- protein
- gene
- cell
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.ショウジョウバエのパッチド遺伝子以外のパッチド遺伝子をコードする染 色体に存在する以外のDNA 配列、又はショウジョウバエのパッチド遺伝子の配列 とは異なる少なくとも約12bpのそのフラグメント。 2.前記パッチド遺伝子が哺乳類遺伝子である請求の範囲第1項記載のDNA 配 列。 3.ヒト、マウス、蚊、チョウ又は甲虫のパッチド遺伝子のための請求の範囲 第1項記載のDNA 配列。 4.前記DNA 配列がヒト配列である請求の範囲第3項記載のDNA 配列。 5.前記DNA 配列がマウス配列である請求の範囲第4項記載のDNA 配列。 6.前記DNA 配列が少なくとも約18bpのフラグメントである請求の範囲第1項 記載のDNA 配列。 7.制限酵素認識配列を含んで成るDNA 配列に連結される請求の範囲第1項記 載のDNA 配列。 8.発現宿主において機能的な転写開始領域、前記転写開始領域の転写調節下 での請求の範囲第1項記載のDNA 配列、及び前記発現宿主において機能的な転写 終結領域を含んで成る発現カセット。 9.前記転写開始領域が請求の範囲第1項記載のDNA 配列に対して異種である 請求の範囲第8項記載の発現カセット。 10.前記転写開始領域が請求の範囲第1項記載のDNA 配列に対して同種であり 、そしてエンハンサー領域を含む請求の範囲第8項記載の発現カセット。 11.染色体外要素の一部としての請求の範囲第1項記載の発現カ セット又は宿主細胞及び前記宿主細胞の細胞性子孫中への前記発現カセットの導 入の結果として前記宿主細胞のゲノム中に組込まれる前記発現カセットを含んで 成る細胞。 12.前記宿主細胞の細胞膜にパッチドタンパク質をさらに含んで成る請求の範 囲第11項記載の細胞。 13.前記パッチドタンパク質がマウスのパッチドタンパク質である請求の範囲 第11項記載の細胞。 14.前記パッチド遺伝子がヒトのパッチド遺伝子である請求の範囲第11項記載 の細胞。 15.前記転写開始領域が、異種遺伝子に連結されるプロモーター及びエンハン サーを含んで成るショウジョウバエのパッチド遺伝子転写開始領域である請求の 範囲第11項記載の細胞。 16.プロモーター及びエンハンサーを含んで成るパッチドタンパク質の転写を 調節する5′非コード領域から成る、発現宿主において機能的な転写開始領域、 マーカー遺伝子、及び転写終結領域を、染色体外要素の一部として、又は前記宿 主及びその細胞性子孫中への前記発現カセットの導入の結果として宿主細胞のゲ ノム中に組込まれるものとして含んで成る発現カセットを含んで成る細胞。 17.前記転写開始領域がショウジョウバエの領域である請求の範囲第16項記載 の細胞。 18.胚にパッチドタンパク質を用いて胚の成長を追跡するための方法であって 、 パッチドタンパク質の転写を調節する5′非−コード領域から成る、成長する ことができる胚縮主細胞において機能的な転写開始領域、マーカー遺伝子、及び 転写終結領域を含んで成る発現カセットを胎児中に組込み; 前記胚の宿主細胞を増殖し、それにより増殖及び分化が生じ;そ して 前記マーカー遺伝子の発現によりパッチドタンパク質の発現を含んで成る細胞 の位置を決定することを含んで成る方法。 19.請求の範囲第11項記載の細胞を増殖し、それによりパッチドタンパク質を 発現し;そして 他のタンパク質を含まない前記パッチドタンパク質を単離することを含んで成 る、パッチドタンパク質を生成するための方法。 20.パッチドタンパク質に対する結合親和性のための候補体化合物をスクリー ンするための方法であって、 前記候補体タンパク質と、脊椎動物又は無脊椎動物の細胞の膜における前記パ ッチドタンパク質、及び前記細胞において機能的な転写開始領域、前記転写開始 領域の転写調節下で完全なコード配列を含んで成る請求の範囲第1項記載のDNA 配列、及び前記細胞において機能的な転写終結領域を含んで成る発現カセットを 含んで成る前記脊椎動物又は無脊椎動物細胞とを組合し、前記パッチドタンパク 質を前記細胞において発現し;そして 前記パッチドタンパク質への前記候補体化合物の結合性についてアッセイする ことを含んで成る方法。 21.パッチドタンパク質によりアゴニスト活性のための候補体化合物をスクリ ーンするための方法であって、 前記候補体タンパク質と、脊椎動物又は無脊椎動物の細胞の膜における前記パ ッチドタンパク質、及びパッチドタンパク質の転写を調節する5′非−コード領 域から成る、発現宿主において機能的な転写開始領域、マーカー遺伝子、及び転 写終結領域を、染色体外要素の一部として又は宿主細胞のゲノム中に組込まれる ものとして含んで成る発現カセットを含んで成る前記脊椎動物又は無脊椎動物細 胞とを組合し;そして 前記マーカー遺伝子の発現についてアッセイすることを含んで成る方法。 22.ショウジョウバエのパッチドタンパク質以外のパッチドタンパク質に特異 的に結合するモノクローナル抗体。
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DE (1) | DE69534310T2 (ja) |
DK (1) | DK0804548T3 (ja) |
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Cited By (1)
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JP2014504730A (ja) * | 2011-01-18 | 2014-02-24 | アメリカ合衆国 | プリオンの増幅および検出のための方法 |
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US6027882A (en) * | 1994-10-07 | 2000-02-22 | The Regents Of The University Of California | Patched genes and their use for diagnostics |
US6429354B1 (en) * | 1994-10-07 | 2002-08-06 | The Board Of Trustees Of The Leland Stanford Junior University | Patched genes and uses related thereto |
JP3776454B2 (ja) | 1994-10-07 | 2006-05-17 | ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ | パッチド遺伝子及びそれらの使用 |
AU752969B2 (en) * | 1994-10-07 | 2002-10-03 | Board Of Trustees Of The Leland Stanford Junior University | Patched genes and their use |
WO1997043414A2 (en) * | 1996-05-17 | 1997-11-20 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | A basal cell carcinoma tumor suppressor gene |
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US5789543A (en) * | 1993-12-30 | 1998-08-04 | President And Fellows Of Harvard College | Vertebrate embryonic pattern-inducing proteins and uses related thereto |
US6610656B1 (en) * | 1993-12-30 | 2003-08-26 | President And Fellows Of Harvard College | Method of promoting chondrocyte differentiation with hedgehog related polypeptides |
US5750367A (en) * | 1993-11-08 | 1998-05-12 | Baylor College Of Medicine | Human and mouse very low density lipoprotein receptors and methods for use of such receptors |
US6384192B1 (en) * | 1993-12-30 | 2002-05-07 | President & Fellows Of Harvard College | Vertebrate embryonic pattern-inducing proteins |
US6165747A (en) * | 1993-12-30 | 2000-12-26 | President & Fellows Of Harvard College | Nucleic acids encoding hedgehog proteins |
US6884775B1 (en) * | 1993-12-30 | 2005-04-26 | President And Fellows Of Harvard College | Methods and compositions for regulating skeletogenic formation |
US6261786B1 (en) * | 1993-12-30 | 2001-07-17 | Imperial Cancer Res. Technology | Screening assays for hedgehog agonists and antagonists |
US7060450B1 (en) * | 1993-12-30 | 2006-06-13 | President And Fellows Of Harvard College | Screening assays for agonists and antagonists of the hedgehog signaling pathway |
US20030186357A1 (en) * | 1993-12-30 | 2003-10-02 | Philip W. Ingham | Vertebrate embryonic pattern-inducing proteins, and uses related thereto |
US6001968A (en) | 1994-08-17 | 1999-12-14 | The Rockefeller University | OB polypeptides, modified forms and compositions |
US6946257B1 (en) * | 1994-10-07 | 2005-09-20 | Regents Of The University Of California | Patched genes and uses related thereto |
US6027882A (en) | 1994-10-07 | 2000-02-22 | The Regents Of The University Of California | Patched genes and their use for diagnostics |
JP3776454B2 (ja) * | 1994-10-07 | 2006-05-17 | ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ | パッチド遺伝子及びそれらの使用 |
US6429354B1 (en) * | 1994-10-07 | 2002-08-06 | The Board Of Trustees Of The Leland Stanford Junior University | Patched genes and uses related thereto |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014504730A (ja) * | 2011-01-18 | 2014-02-24 | アメリカ合衆国 | プリオンの増幅および検出のための方法 |
JP2017134080A (ja) * | 2011-01-18 | 2017-08-03 | アメリカ合衆国 | プリオンの増幅および検出のための方法 |
Also Published As
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US6172200B1 (en) | 2001-01-09 |
DK0804548T3 (da) | 2005-11-07 |
AU4001195A (en) | 1996-05-02 |
ATE299529T1 (de) | 2005-07-15 |
EP0804548A4 (en) | 1999-07-21 |
US7659371B2 (en) | 2010-02-09 |
ES2245454T3 (es) | 2006-01-01 |
US20030032085A1 (en) | 2003-02-13 |
CA2201887C (en) | 2009-04-14 |
CA2201887A1 (en) | 1996-04-18 |
EP1616942A3 (en) | 2006-11-22 |
EP0804548A1 (en) | 1997-11-05 |
US20030148388A1 (en) | 2003-08-07 |
US5837538A (en) | 1998-11-17 |
DE69534310D1 (de) | 2005-08-18 |
EP1616942A2 (en) | 2006-01-18 |
US6610507B2 (en) | 2003-08-26 |
EP0804548B1 (en) | 2005-07-13 |
US6921646B2 (en) | 2005-07-26 |
WO1996011260A1 (en) | 1996-04-18 |
JP3776454B2 (ja) | 2006-05-17 |
US20100221724A1 (en) | 2010-09-02 |
US8178298B2 (en) | 2012-05-15 |
US20080300167A1 (en) | 2008-12-04 |
DE69534310T2 (de) | 2006-04-27 |
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