JPH10500847A - 乳頭腫ウィルスワクチン - Google Patents
乳頭腫ウィルスワクチンInfo
- Publication number
- JPH10500847A JPH10500847A JP7529808A JP52980895A JPH10500847A JP H10500847 A JPH10500847 A JP H10500847A JP 7529808 A JP7529808 A JP 7529808A JP 52980895 A JP52980895 A JP 52980895A JP H10500847 A JPH10500847 A JP H10500847A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- papillomavirus
- cell
- recombinant
- virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. L1タンパク質、L2タンパク質、L1+L2タンパク質およびそれらの 機能的誘導体から選択される乳頭腫ウィルスの単離および精製された少なくとも 60%の純度を有するタンパク質。 2. 乳頭腫ウィルスがヒト乳頭腫ウィルスおよびワタオウサギ乳頭腫ウィルス からなる群より選択される請求項1に記載のタンパク質。 3. ヒト乳頭腫ウィルスが、HPV6a、HPV6b、HPV11、HPV1 6、HPV18、HPV31、HPV33、HPV35、HPV41、HPV4 5およびHPV58からなる群より選択される請求項2に記載のタンパク質 4. タンパク質が組換え宿主からなる合成系により製造される請求項1に記載 のタンパク質。 5. 請求項1記載のタンパク質からなるカプシド。 6. 請求項1記載のタンパク質からなるウィルス状粒子。 7. (a)乳頭腫ウィルスL1タンパク質、乳頭腫ウィルスL2タンパク質、 乳頭腫ウィルスL1+L2タンパク質およ びそれらの機能的誘導体から選択されるタンパク質をコードする組換えDNA分 子で宿主を形質転換し、 (b)組換えDNA分子の発現を可能にする条件下に形質転換宿主を培養して 粗組換え乳頭腫ウィルスタンパク質を生成し、および (c)粗組換え乳頭腫ウィルスタンパク質を精製して精製タンパク質を生成す る、 ことを含む、請求項1記載の精製タンパク質の製造方法。 8. 請求項7記載の方法により製造された組換え乳頭腫ウィルスタンパク質。 9. 請求項7記載のタンパク質からなるカプシド。 10. 請求項7記載のタンパク質からなるウィルス状粒子。 11. 請求項1記載のタンパク質を含むワクチン。 12. 請求項1記載のタンパク質を含む医薬組成物。 13. 請求項11記載のワクチンを宿主に投与することを含む、乳頭腫ウィル ス感染を治療または予防する方法。 14. 請求項1記載の乳頭腫ウィルスタンパク質をコードするDNA分子、請 求項1記載のタンパク質、請求項1記載のタンパク質からなるウィルス状粒子、 請求項1記載のタンパクに 対する抗体、および請求項1記載のタンパク質からなるウィルス状粒子に対する 抗体から選択される試薬を含む、乳頭腫ウィルスまたは乳頭腫ウィルスに対する 抗体を検出するためのキット。 15. (a)少なくとも一つの乳頭腫ウィルスカプシドタンパク質をコードす る乳頭腫ウィルス遺伝子をベクター内にクローニングし、 (b)ベクターを宿主細胞内に移入して組換え宿主細胞を生成し、 (c)乳頭腫ウィルスカプシドタンパク質の生成を可能にする条件下に組換え 宿主細胞を培養し、および (d)乳頭腫ウィルスカプシドタンパク質を精製する、 ことを含む、カプシドまたはウィルス状粒子に組み立てられた組換え乳頭腫ウィ ルスカプシドタンパク質を製造する方法。 16. 請求項15記載の方法により製造されるカプシド。 17. 請求項15記載の方法により製造されるウィルス状粒子。 18. 請求項15記載の精製タンパク質、または請求項15記載のカプシド、 または請求項15記載のウィルス状粒子を動 物に投与することを含む、脊椎動物において免疫反応を誘発するための方法。 19. 請求項1記載のタンパク質を動物に投与することを含む、動物において 免疫反応を誘発するための方法。 20. 形質転換された昆虫細胞、形質転換された酵母細胞、形質転換された細 菌細胞、形質転換された真菌細胞、形質転換された植物細胞および形質転換され た動物細胞から選択される請求項4に記載の合成系。 21. 酵母が、Saccharomyces cerevisiae、Sac charomyces carlsbergensis、Hansenula polymorpha 、Pichia pastoris、Kluyvermy ces fragilis、およびSchizosaccharomyces pombe からなる群より選択される請求項20に記載の合成系。 22. Saccharomyces cerevisiaeが、菌株2150 −2−3、U9、1372、1372−ura3、1372−his3、155 8、1524、1537、 1541、1582および1569からなる群より選択される請求項21に記載 の合成系。
Applications Claiming Priority (3)
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US24279494A | 1994-05-16 | 1994-05-16 | |
US242,794 | 1994-05-16 | ||
PCT/US1995/006006 WO1995031532A1 (en) | 1994-05-16 | 1995-05-15 | Papillomavirus vaccines |
Publications (2)
Publication Number | Publication Date |
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JPH10500847A true JPH10500847A (ja) | 1998-01-27 |
JP3863559B2 JP3863559B2 (ja) | 2006-12-27 |
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JP52980895A Expired - Lifetime JP3863559B2 (ja) | 1994-05-16 | 1995-05-15 | 乳頭腫ウィルスワクチン |
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EP (1) | EP0757717B1 (ja) |
JP (1) | JP3863559B2 (ja) |
CN (1) | CN1152935A (ja) |
AT (1) | ATE328068T1 (ja) |
AU (1) | AU693203B2 (ja) |
BG (1) | BG100974A (ja) |
BR (1) | BR9507657A (ja) |
CA (1) | CA2189882C (ja) |
CZ (1) | CZ336696A3 (ja) |
DE (1) | DE69535018T2 (ja) |
DK (1) | DK0757717T3 (ja) |
ES (1) | ES2264126T3 (ja) |
FI (1) | FI119815B (ja) |
HU (1) | HUT76354A (ja) |
MX (1) | MXPA96005662A (ja) |
NO (1) | NO322133B1 (ja) |
NZ (1) | NZ285941A (ja) |
PL (1) | PL183781B1 (ja) |
PT (1) | PT757717E (ja) |
RO (1) | RO117541B1 (ja) |
RU (1) | RU2206608C2 (ja) |
SI (1) | SI0757717T1 (ja) |
SK (1) | SK147696A3 (ja) |
WO (1) | WO1995031532A1 (ja) |
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- 1995-05-15 JP JP52980895A patent/JP3863559B2/ja not_active Expired - Lifetime
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JP2011037902A (ja) * | 1998-12-11 | 2011-02-24 | Univ Of Queensland | パピローマウイルス感染の治療方法 |
JP2002532434A (ja) * | 1998-12-11 | 2002-10-02 | ザ ユニバーシティ オブ クイーンズランド | パピローマウイルス感染の治療方法 |
JP2016136947A (ja) * | 2003-09-29 | 2016-08-04 | メルク・シャープ・アンド・ドーム・コーポレーションMerck Sharp & Dohme Corp. | 酵母におけるhpv45l1の最適化された発現 |
JP2014221052A (ja) * | 2003-09-29 | 2014-11-27 | メルク・シャープ・アンド・ドーム・コーポレーションMerck Sharp & Dohme Corp. | 酵母におけるhpv45l1の最適化された発現 |
JP2007507207A (ja) * | 2003-09-29 | 2007-03-29 | メルク エンド カムパニー インコーポレーテッド | 酵母におけるhpv45l1の最適化された発現 |
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Also Published As
Publication number | Publication date |
---|---|
DK0757717T3 (da) | 2006-09-25 |
DE69535018T2 (de) | 2007-02-15 |
AU693203B2 (en) | 1998-06-25 |
EP0757717B1 (en) | 2006-05-31 |
PL183781B1 (pl) | 2002-07-31 |
NO322133B1 (no) | 2006-08-21 |
CA2189882C (en) | 2005-09-20 |
CN1152935A (zh) | 1997-06-25 |
BR9507657A (pt) | 1997-09-09 |
NO964862D0 (no) | 1996-11-15 |
NZ285941A (en) | 1998-05-27 |
NO964862L (no) | 1997-01-16 |
AU2549595A (en) | 1995-12-05 |
ES2264126T3 (es) | 2006-12-16 |
HUT76354A (en) | 1997-08-28 |
WO1995031532A1 (en) | 1995-11-23 |
ATE328068T1 (de) | 2006-06-15 |
EP0757717A4 (en) | 1998-04-15 |
HU9603155D0 (en) | 1997-01-28 |
EP0757717A1 (en) | 1997-02-12 |
FI119815B (fi) | 2009-03-31 |
PT757717E (pt) | 2006-09-29 |
SI0757717T1 (sl) | 2006-08-31 |
CA2189882A1 (en) | 1995-11-23 |
DE69535018D1 (de) | 2006-07-06 |
CZ336696A3 (en) | 1997-07-16 |
BG100974A (en) | 1998-04-30 |
SK147696A3 (en) | 1997-08-06 |
FI964592A0 (fi) | 1996-11-15 |
JP3863559B2 (ja) | 2006-12-27 |
PL317234A1 (en) | 1997-03-17 |
FI964592A (fi) | 1996-11-15 |
RU2206608C2 (ru) | 2003-06-20 |
MXPA96005662A (es) | 2004-08-19 |
RO117541B1 (ro) | 2002-04-30 |
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