JPH08509694A - 遺伝物質送達のための組成物および方法 - Google Patents
遺伝物質送達のための組成物および方法Info
- Publication number
- JPH08509694A JPH08509694A JP6517285A JP51728594A JPH08509694A JP H08509694 A JPH08509694 A JP H08509694A JP 6517285 A JP6517285 A JP 6517285A JP 51728594 A JP51728594 A JP 51728594A JP H08509694 A JPH08509694 A JP H08509694A
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- Prior art keywords
- hiv
- protein
- nucleic acid
- dna
- individual
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. 個体の細胞内に遺伝物質を導入する方法であって、 a)該個体の細胞とポリヌクレオチド機能促進剤とを接触させ; b)該個体の細胞に核酸分子を投与する; の各工程を含み、該核酸分子はレトロウイルス粒子を含まないことを特徴とする 方法。 2. 該ポリヌクレオチド機能促進剤がブピバカインである請求項第1項に記載 の方法。 3. 該核酸分子が、蛋白質をコードしかつ作動可能なように制御配列に連結さ れているヌクレオチド配列を含む、請求項第1項に記載の方法。 4. 該核酸分子が、それに対する免疫応答が望まれる抗原のエピトープと同一 または実質的に同じである少なくとも一つのエピトープを含む蛋白質をコードす るヌクレオチド配列を含み、該ヌクレオチド配列は作動可能なように制御配列に 連結されている、請求項第1項に記載の方法。 5. 病原体に対して個体を免疫処置する方法であって、 a)該個体の細胞とポリヌクレオチド機能促進剤とを接触させ; b)該個体の細胞に、病原体抗原のエピトープと同一または実質的に同じ である少なくとも一つのエピトープを含む蛋白質をコードするヌクレオチド配列 を含む核酸分子を投与し、該ヌクレオチドは作動可能なように制御配列に連結さ れている; の各工程を含み、該核酸分子はレトロウイルス粒子を含まず、かつ該ヌクレオチ ド配列は該細胞中で発現されることができることを特徴とする方法。 6. 該ポリヌクレオチド機能促進剤がブピバカインである請求項第5項に記載 の方法。 7. 該核酸分子がDNA分子である請求項第5項に記載の方法。 8. 該蛋白質が病原体抗原またはその断片である請求項第5項に記載の方法。 9. 該核酸分子が筋肉内に投与される請求項第5項に記載の方法。 10.該病原体が、ヒト免疫不全ウイルス、HIV;ヒトT細胞白血病ウイルス 、 HTLV;インフルエンザウイルス;A型肝炎ウイルス、HAV;B型肝炎ウイ ルス、HBV;C型肝炎ウイルス、HCV;ヒト乳頭腫ウイルス、HPV;単純 ヘルペス1ウイルス、HSV1;単純ヘルペス2ウイルス、HSV2;サイトメ ガロウイルス、CMV;エプスタイン−バールウイルス、EBV;ライノウイル ス;およびコロナウイルスからなる群より選択されるウイルスである、請求項第 5項に記載の方法。 11.該病原体がHIVであり、該核酸分子がHIV蛋白質をコードするヌクレ オチド配列を含む、請求項第5項に記載の方法。 12.該病原体がHIVであり、該核酸分子が2つ以上のHIV構造蛋白質をコ ードするヌクレオチド配列を含む、請求項第5項に記載の方法。 13.該病原体がHIVであり、該核酸分子が2つ以上のHIV制御蛋白質をコ ードするヌクレオチド配列を含む、請求項第5項に記載の方法。 14.少なくとも二つまたはそれ以上の異なる核酸分子が個体の異なる細胞に投 与され、該異なる核酸分子は各々同じ病原体の一つまたはそれ以上の病原体抗原 をコードするヌクレオチド配列を含む、請求項第5項に記載の方法 15.該ポリヌクレオチド機能促進剤および該核酸分子が同時に投与される請求 項第5項に記載の方法。 16.該個体がヒトであり; 該ポリヌクレオチド機能促進剤がブピバカインであり; 該病原体がヒト免疫不全ウイルスであり; 該核酸分子がDNAであり、psiを欠失しているHIV構造蛋白質gagお よびpolをコードするDNA配列を含み、該gagおよびpolをコードする DNA配列がラウス肉腫ウイルスエンハンサー、サイトメガロウイルス前初期プ ロモーターおよびSV40マイナーポリアデニル化シグナルおよび随意にSV4 0複製開始点に作動可能なように連結されている、請求項第5項に記載の方法。 17.該DNA配列がさらにHIVrev応答要素を含みおよびHIVインテグ ラーゼが欠損している請求項第16項に記載の方法。 18.該DNA配列がさらにHIVスプライスアクセプターを含む請求項第17 項に記載の方法。 19.該DNA分子が、さらにSV40プロモーター、SV40マイナーポリア デニル化シグナルおよび随意にSV40複製開始点に作動可能なように連結され ているHIVrevをさらに含む配列をコードするDNA配列をさらに含む、請 求項第16項に記載の方法。 20.該revをコードするDNA配列がさらにHIVvpuおよびHIVen v をコードする、請求項第19項に記載の方法。 21.該gagおよびpolをコードするDNA配列がさらにHIVrev応答 要素を含み、かつHIVインテグラーゼを欠失している、請求項第19項に記載 の方法。 22.該gagおよびpolをコードするDNA配列がさらにHIVスプライス アクセプターを含む、請求項第21項に記載の方法。 23.該個体がヒトであり; 該ポリヌクレオチド機能促進剤がブピバカインであり; 該病原体がヒト免疫不全ウイルスであり; 該核酸分子がDNAであり、かつラウス肉腫ウイルスエンハンサー、サイトメ ガロウイルス前初期プロモーターおよびSV40マイナーポリアデニル化シグナ ルおよび随意にSV40複製開始点に作動可能なように連結されているHIV構 造蛋白質rev、vpuおよびenvをコードするDNA配列を含む、請求項第 5項に記載の方法。 24.該個体がヒトであり; 該ポリヌクレオチド機能促進剤がブピバカインであり; 該病原体がヒト免疫不全ウイルスであり; 二つの異なるDNA分子が個体の異なる細胞に投与され; 該核酸分子の一方がDNAであり、psiを欠失したHIV構造蛋白質gag およびpolをコードするDNA配列を含み、該gagおよびpolをコードす るDNA配列がラウス肉腫ウイルスエンハンサー、サイトメガロウイルス前初期 プロモーターおよびSV40マイナーポリアデニル化シグナルおよび随意にSV 40複製開始点に作動可能なように連結されており; 該核酸分子の他方がDNAであり、かつラウス肉腫ウイルスエンハンサー、サ イトメガロウイルス前初期プロモーターおよびSV40マイナーポリアデニル化 シグナルおよび随意にSV40複製開始点に作動可能なように連結されているH IV構造蛋白質rev、vpuおよびenvをコードするDNA配列を含む、請 求項第5項に記載の方法。 25.HIVに対してヒトを免疫処置する方法であって、二つの異なる核酸分子 を該ヒトの細胞に投与する工程を含み;該核酸分子の各々は、制御配列に作動可 能なように連結され、少なくとも一つのHIV抗原のエピトープと同一であるか または実質的に同じである少なくとも一つのエピトープを含む蛋白質をコードす るヌクレオチド配列を含み;該ヌクレオチド配列は該細胞中で発現されることが 可能であり;該異なる核酸分子の各々のヌクレオチド配列は異なる蛋白質をコー ドしており;該蛋白質は、gag、polおよびenvからなる群から選択され るHIV遺伝子によりコードされた少なくとも一つのHIV蛋白質のエピトープ と同一または実質的に同等の少なくとも一つのエピトープを含むことを特徴とす る方法。 26.疾患に対して個体を免疫処置する方法であって、 a)該個体の細胞とポリヌクレオチド機能促進剤とを接触させ; b)該個体の細胞に、作動可能なように制御配列に連結され、該疾患を特 徴付ける細胞と関連する蛋白質のエピトープと同一または実質的に同じであるエ ピトープを含む標的蛋白質をコードするヌクレオチド配列を含む核酸分子を投与 する; の各工程を含み、該核酸分子はレトロウイルス粒子を含まず、かつ該細胞で発現 されることが可能であることを特徴とする方法。 27.該ポリヌクレオチド機能促進剤がブピバカインである請求項第26項に記 載の方法。 28.該疾患が過増殖性細胞により特徴付けられる請求項第26項に記載の方法 。 29.該疾患が自己免疫疾患である請求項第26項に記載の方法。 30.該核酸分子がDNA分子である請求項第26項に記載の方法。 31.該核酸分子が筋肉内に投与される請求項第26項に記載の方法。 32.該核酸分子が、癌遺伝子myb、myc、fyn、ras、sarc、n eu およびtrkの蛋白質生成物;転座遺伝子bcl/ablの蛋白質生成物; P53;EGRF;B細胞リンパ腫により作られる抗体の可変領域;およびT細 胞リンパ腫のT細胞受容体の可変領域からなる群より選択される標的蛋白質をコ ードするヌクレオチド配列を含む、請求項第26項に記載の方法。 33.該蛋白質がB細胞介在自己免疫疾患に関与する抗体の可変領域;およびT 細胞介在自己免疫疾患に関与するT細胞受容体の可変領域からなる群より選択さ れる、請求項第26項に記載の方法。 34.医薬免疫処置キットであって、 a) i)医薬として受容可能な担体または希釈剤;および ii)作動可能なように制御遺伝子に連結され、少なくとも一つのHI V蛋白質をコードするヌクレオチド配列を含む第一の核酸分子;ここで該ヌクレ オチド配列はヒト細胞中で発現されることが可能である; を含む第一の接種物: b) i)医薬として受容可能な担体または希釈剤;および ii)作動可能なように制御遺伝子に連結され、少なくとも一つのHI V蛋白質をコードするヌクレオチド配列を含む第二の核酸分子;ここで該ヌクレ オチド配列はヒト細胞中で発現されることが可能である; を含む第二の接種物: を含み、該第一の核酸分子は該第二の核酸分子と同一ではないことを特徴とする キット。 35.さらに c)ブピバカインを含む第三の接種物 を含む、請求項第34項に記載の医薬キット。 36.該第一の核酸分子および該第二の核酸分子が、合わせてHIV蛋白質ga g 、polおよびenvをコードする、請求項第34項に記載の医薬組成物。 37.a)psiを欠失したHIV構造蛋白質gagおよびpolをコードする DNA配列を含むDNA分子、ここで該gagおよびpolをコードするDNA 配列は、ラウス肉腫ウイルスエンハンサー、サイトメガロウイルス前初期プロモ ーターおよびSV40マイナーポリアデニル化シグナルおよび随意にSV40複 製開始点に作動可能なように連結されている;および b)ポリヌクレオチド機能促進剤 を含む医薬組成物。 38.該細胞刺激化合物がブピバカインである請求項第37項に記載の医薬組成 物。 39.該DNA配列がさらにHIVrev応答要素を含み、HIVインテグラー ゼを欠失している、請求項第37項に記載の医薬組成物。 40 該DNA配列がさらにHIVスプライスアクセプターを含む請求項第37 項に記載の医薬組成物。 41.該DNA分子がSV40プロモーターおよびSV40マイナーポリアデニ ル化シグナルおよび随意にSV40複製開始点に作動可能なように連結されてい るHIVrevをさらに含む配列をコードするDNA配列をさらに含む、請求項 第37項に記載の医薬組成物。 42.該revをコードするDNA配列がさらにHIVvpuおよびHIVen v をコードする、請求項第41項に記載の医薬組成物。 43.該gagおよびpolをコードするDNA配列がさらにHIVrev応答 要素を含み、HIVインテグラーゼを欠失している、請求項第42項に記載の医 薬組成物。 44.該gagおよびpolをコードするDNA配列がさらにHIVスプライス アクセプターを含む、請求項第42項に記載の医薬組成物。 45.a)ラウス肉腫ウイルスエンハンサー、サイトメガロウイルス前初期プロ モーターおよびSV40マイナーポリアデニル化シグナルおよび随意にSV40 複製開始点に作動可能なように連結されているHIV構造蛋白質rev、vpu およびenvをコードするDNA配列を含むDNA分子;および b)ポリヌクレオチド機能促進剤。 を含む医薬組成物。 46.該ポリペプチド機能促進剤がブピバカインである請求項第45項に記載の 医薬組成物。 47.a) i)psiを欠失しているHIV構造蛋白質gagおよびpolを コードするDNA配列を含むDNA分子を含み、該gagおよびpolをコード する該DNA配列はラウス肉腫ウイルスエンハンサー、サイトメガロウイルス前 初期プロモーターおよびSV40マイナーポリアデニル化シグナルおよび随意に SV40複製開始点に作動可能なように連結されている第一の医薬組成物、およ び ii)ポリヌクレオチド機能促進剤; を含む第一の接種物:および b) i)ラウス肉腫ウイルスエンハンサー、サイトメガロウイルス前初 期プロモーターおよびSV40マイナーポリアデニル化シグナルおよび随意にS V40複製開始点に作動可能なように連結されているHIV蛋白質rev、vp u およびenvをコードするDNA配列を含むDNA分子を含む第二の医薬組成 物;および ii)ポリヌクレオチド機能促進剤。 を含む第二の接種物: を含む医薬免疫処置キット。 48.該ポリペプチド機能促進剤がブピバカインである請求項第47項に記載の 医薬免疫処置キット。 49.疾患を有すると疑われる個体を処置する方法であって、 a)該個体の細胞をポリヌクレオチド機能促進剤と接触させ; b)該個体の細胞に、失われた、機能を果たさないまたは部分的にしか機能し ない蛋白質をその存在が補償し、または個体に治療的効果を生じさせるであろう 蛋白質をコードするヌクレオチド配列を含む核酸分子を投与し、ここで該ヌクレ オチド配列は作動可能なように制御配列に連結されている; の各工程を含み、該核酸分子はレトロウイルス粒子を含まず、かつ該細胞中で発 現されることが可能であることを特徴とする方法。 50.該ポリペプチド機能促進剤がブピバカインである請求項第49項に記載の 方法。 51.該核酸分子がDNA分子である請求項第49項に記載の方法。 52.該核酸分子が筋肉内に投与される請求項第49項に記載の方法。 53.該核酸分子が、酵素、構造蛋白質、サイトカイン、リンホカインおよび増 殖因子からなる群より選択される蛋白質をコードするヌクレオチド配列を含む請 求項第49項に記載の方法。 54.i)病原体抗原のエピトープと同一であるかまたは実質的に同じである少 なくとも一つのエピトープを含む蛋白質;過増殖性細胞に関連する蛋白質のエピ トープと同一であるかまたは実質的に同じであるエピトープを含む蛋白質;自己 免疫疾患を特徴付ける細胞に関連する蛋白質のエピトープと同一であるかまたは 実質的に同じであるエピトープを含む蛋白質;個体において、失われた、機能を 果たさないまたは部分的にしか機能しない蛋白質をその存在が補償するであろう 蛋白質;および個体に治療的効果を生み出す蛋白質からなる群より選択される蛋 白質をコードするヌクレオチド配列を含む核酸分子;および ii)ポリヌクレオチド機能促進剤; を含み、レトロウイルス粒子を含まないことを特徴とする医薬組成物。 55.該ポリペプチド機能促進剤がブピバカインである請求項第54項に記載の 方法。 56.i)病原体抗原のエピトープと同一であるかまたは実質的に同じである少 なくとも一つのエピトープを含む蛋白質;過増殖性細胞に関連する蛋白質のエピ トープと同一であるかまたは実質的に同じであるエピトープを含む蛋白質;自己 免疫疾患を特徴付ける細胞に関連する蛋白質のエピトープと同一であるかまたは 実質的に同じであるエピトープを含む蛋白質;個体において、失われた、機能を 果たさないまたは部分的にしか機能しない蛋白質をその存在が補償するであろう 蛋白質;および個体に治療的効果を生み出す蛋白質からなる群より選択される蛋 白質をコードするヌクレオチド配列を含む核酸分子を含む容器;および ii)ポリヌクレオチド機能促進剤を含む容器; を含み、レトロウイルス粒子を含まないことを特徴とする医薬キット。 57.該ポリペプチド機能促進剤がブピバカインである請求項第56項に記載の 方法。
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1994
- 1994-01-26 ES ES94909492T patent/ES2249760T3/es not_active Expired - Lifetime
- 1994-01-26 JP JP51728594A patent/JP3701966B2/ja not_active Expired - Lifetime
- 1994-01-26 PT PT94909492T patent/PT681483E/pt unknown
- 1994-01-26 AU AU62320/94A patent/AU675702B2/en not_active Expired
- 1994-01-26 CA CA2153593A patent/CA2153593C/en not_active Expired - Lifetime
- 1994-01-26 WO PCT/US1994/000899 patent/WO1994016737A1/en active IP Right Grant
- 1994-01-26 HU HU9502229A patent/HU219767B/hu not_active IP Right Cessation
- 1994-01-26 AT AT94909492T patent/ATE302854T1/de active
- 1994-01-26 DK DK94909492T patent/DK0681483T3/da active
- 1994-01-26 EP EP94909492A patent/EP0681483B1/en not_active Expired - Lifetime
- 1994-01-26 IL IL108449A patent/IL108449A/en not_active IP Right Cessation
- 1994-01-26 NZ NZ262582A patent/NZ262582A/en not_active IP Right Cessation
- 1994-01-26 DE DE69434469T patent/DE69434469T2/de not_active Expired - Lifetime
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003518076A (ja) * | 1999-12-22 | 2003-06-03 | ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア | コスミドdna構築物およびその作製および使用方法 |
JP2008517918A (ja) * | 2004-10-21 | 2008-05-29 | ワイス | Staphylococcusepidermidisポリペプチド抗原の免疫原性組成物 |
JP2013121981A (ja) * | 2004-10-21 | 2013-06-20 | Wyeth Llc | Staphylococcusepidermidisポリペプチド抗原の免疫原性組成物 |
Also Published As
Publication number | Publication date |
---|---|
EP0681483B1 (en) | 2005-08-24 |
HUT73099A (en) | 1996-06-28 |
CA2153593C (en) | 2010-04-13 |
ES2249760T3 (es) | 2006-04-01 |
CA2153593A1 (en) | 1994-08-04 |
HU219767B (hu) | 2001-07-30 |
NZ262582A (en) | 1997-10-24 |
AU675702B2 (en) | 1997-02-13 |
EP0681483A1 (en) | 1995-11-15 |
DE69434469D1 (de) | 2005-09-29 |
EP0681483A4 (en) | 1997-06-25 |
PT681483E (pt) | 2005-11-30 |
DE69434469T2 (de) | 2006-06-14 |
US20060222629A1 (en) | 2006-10-05 |
DK0681483T3 (da) | 2005-11-21 |
AU6232094A (en) | 1994-08-15 |
IL108449A0 (en) | 1994-04-12 |
HU9502229D0 (en) | 1995-09-28 |
ATE302854T1 (de) | 2005-09-15 |
JP3701966B2 (ja) | 2005-10-05 |
IL108449A (en) | 2011-09-27 |
WO1994016737A1 (en) | 1994-08-04 |
US8304234B2 (en) | 2012-11-06 |
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