JPH08505231A - 粒子自体の凝集を伴わない光散乱に基づく免疫検定 - Google Patents
粒子自体の凝集を伴わない光散乱に基づく免疫検定Info
- Publication number
- JPH08505231A JPH08505231A JP6515382A JP51538294A JPH08505231A JP H08505231 A JPH08505231 A JP H08505231A JP 6515382 A JP6515382 A JP 6515382A JP 51538294 A JP51538294 A JP 51538294A JP H08505231 A JPH08505231 A JP H08505231A
- Authority
- JP
- Japan
- Prior art keywords
- analyte
- microspheres
- monodisperse
- histogram
- coated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 10
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- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 6
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- 241000283690 Bos taurus Species 0.000 description 2
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 2
- 239000005062 Polybutadiene Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000009471 action Effects 0.000 description 2
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- 210000001124 body fluid Anatomy 0.000 description 2
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- 241000283707 Capra Species 0.000 description 1
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- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- 206010029719 Nonspecific reaction Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
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- 235000015115 caffè latte Nutrition 0.000 description 1
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- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 1
- 229960005156 digoxin Drugs 0.000 description 1
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
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- 102000047688 human TG Human genes 0.000 description 1
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- 150000002736 metal compounds Chemical class 0.000 description 1
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- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
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- 229910052700 potassium Inorganic materials 0.000 description 1
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- 238000013374 right angle light scattering Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
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- 239000011780 sodium chloride Substances 0.000 description 1
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- 229940034208 thyroxine Drugs 0.000 description 1
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/553—Metal or metal coated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Inorganic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US99490392A | 1992-12-22 | 1992-12-22 | |
| US07/994,903 | 1992-12-22 | ||
| PCT/US1993/012428 WO1994015193A1 (en) | 1992-12-22 | 1993-12-22 | Light scatter-based immunoassay without particle self aggregation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH08505231A true JPH08505231A (ja) | 1996-06-04 |
Family
ID=25541198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6515382A Pending JPH08505231A (ja) | 1992-12-22 | 1993-12-22 | 粒子自体の凝集を伴わない光散乱に基づく免疫検定 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5589401A (en, 2012) |
| EP (1) | EP0676045A4 (en, 2012) |
| JP (1) | JPH08505231A (en, 2012) |
| KR (1) | KR0171452B1 (en, 2012) |
| CN (1) | CN1061142C (en, 2012) |
| AU (1) | AU670489B2 (en, 2012) |
| CA (1) | CA2151060A1 (en, 2012) |
| TW (1) | TW239881B (en, 2012) |
| WO (1) | WO1994015193A1 (en, 2012) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002540383A (ja) * | 1999-01-25 | 2002-11-26 | ミナーヴァ・バイオテクノロジーズ・コーポレーション | 神経変性疾患における異常型タンパク質凝集の迅速かつ高感度の検出 |
| JP2011095265A (ja) * | 1999-01-23 | 2011-05-12 | Minerva Biotechnologies Corp | コロイド固定化種と非コロイド構造上の種との相互作用 |
Families Citing this family (64)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6200820B1 (en) * | 1992-12-22 | 2001-03-13 | Sienna Biotech, Inc. | Light scatter-based immunoassay |
| WO1998010267A1 (en) * | 1996-09-04 | 1998-03-12 | Technical University Of Denmark | A micro flow system for particle separation and analysis |
| US6165798A (en) * | 1996-10-10 | 2000-12-26 | University Of British Columbia | Optical quantification of analytes in membranes |
| US5858648A (en) * | 1996-11-04 | 1999-01-12 | Sienna Biotech, Inc. | Assays using reference microparticles |
| GB9707096D0 (en) | 1997-04-08 | 1997-05-28 | Smithkline Beecham Plc | Novel device |
| EP0919568A1 (en) | 1997-12-01 | 1999-06-02 | Sorin Diagnostics S.r.l. | Escape mutant of the surface antigen of hepatitis B virus |
| US6127130A (en) | 1998-03-13 | 2000-10-03 | The United States Of America As Represented By The Secretary Of The Navy | Multiassay method for determining the concentrations of antigens and interferants |
| CA2254223A1 (en) * | 1998-11-16 | 2000-05-16 | Biophys, Inc. | Device and method for analyzing a biologic sample |
| US6221579B1 (en) | 1998-12-11 | 2001-04-24 | Kimberly-Clark Worldwide, Inc. | Patterned binding of functionalized microspheres for optical diffraction-based biosensors |
| US20050148101A1 (en) * | 1999-01-23 | 2005-07-07 | Bamdad Cynthia C. | Interaction of colloid-immobilized species with species on non-colloidal structures |
| FR2797690B1 (fr) * | 1999-08-20 | 2001-10-12 | Bio Merieux | Procede de detection d'un analyte et necessaire pour la mise en oeuvre de ce procede |
| US6392327B1 (en) | 2000-03-29 | 2002-05-21 | James L. Sackrison | Sonic transducer and feedback control method thereof |
| WO2001096604A2 (en) * | 2000-06-12 | 2001-12-20 | Genicon Sciences Corporation | Assay for genetic polymorphisms using scattered light detectable labels |
| DE60125388T2 (de) * | 2000-10-03 | 2007-09-27 | Minerva Biotechnologies Corp., Newton | Magnetisches in situ dilutionsverfahren |
| WO2002057743A2 (en) * | 2001-01-17 | 2002-07-25 | Streamline Proteomics, Llc. | Methods of analyzing and sorting one or more analytes |
| US7939283B2 (en) * | 2001-11-01 | 2011-05-10 | Fisher Scientific Company L.L.C. | Analyte binding turbidity assay |
| US7102752B2 (en) | 2001-12-11 | 2006-09-05 | Kimberly-Clark Worldwide, Inc. | Systems to view and analyze the results from diffraction-based diagnostics |
| US7098041B2 (en) | 2001-12-11 | 2006-08-29 | Kimberly-Clark Worldwide, Inc. | Methods to view and analyze the results from diffraction-based diagnostics |
| US8367013B2 (en) | 2001-12-24 | 2013-02-05 | Kimberly-Clark Worldwide, Inc. | Reading device, method, and system for conducting lateral flow assays |
| US6837171B1 (en) | 2002-04-29 | 2005-01-04 | Palmer/Snyder Furniture Company | Lightweight table with unitized table top |
| US20030119203A1 (en) | 2001-12-24 | 2003-06-26 | Kimberly-Clark Worldwide, Inc. | Lateral flow assay devices and methods for conducting assays |
| US7223534B2 (en) | 2002-05-03 | 2007-05-29 | Kimberly-Clark Worldwide, Inc. | Diffraction-based diagnostic devices |
| US7485453B2 (en) | 2002-05-03 | 2009-02-03 | Kimberly-Clark Worldwide, Inc. | Diffraction-based diagnostic devices |
| US7118855B2 (en) | 2002-05-03 | 2006-10-10 | Kimberly-Clark Worldwide, Inc. | Diffraction-based diagnostic devices |
| US7771922B2 (en) | 2002-05-03 | 2010-08-10 | Kimberly-Clark Worldwide, Inc. | Biomolecule diagnostic device |
| US7223368B2 (en) | 2002-05-03 | 2007-05-29 | Kimberly-Clark Worldwide, Inc. | Diffraction-based diagnostic devices |
| US7214530B2 (en) | 2002-05-03 | 2007-05-08 | Kimberly-Clark Worldwide, Inc. | Biomolecule diagnostic devices and method for producing biomolecule diagnostic devices |
| US7091049B2 (en) | 2002-06-26 | 2006-08-15 | Kimberly-Clark Worldwide, Inc. | Enhanced diffraction-based biosensor devices |
| US7285424B2 (en) | 2002-08-27 | 2007-10-23 | Kimberly-Clark Worldwide, Inc. | Membrane-based assay devices |
| US7314763B2 (en) | 2002-08-27 | 2008-01-01 | Kimberly-Clark Worldwide, Inc. | Fluidics-based assay devices |
| US7432105B2 (en) | 2002-08-27 | 2008-10-07 | Kimberly-Clark Worldwide, Inc. | Self-calibration system for a magnetic binding assay |
| JP4087200B2 (ja) * | 2002-09-17 | 2008-05-21 | ユニバーサル・バイオ・リサーチ株式会社 | 粒子複合体及び該粒子複合体の作製方法 |
| US7169550B2 (en) | 2002-09-26 | 2007-01-30 | Kimberly-Clark Worldwide, Inc. | Diffraction-based diagnostic devices |
| US7122384B2 (en) * | 2002-11-06 | 2006-10-17 | E. I. Du Pont De Nemours And Company | Resonant light scattering microparticle methods |
| KR20040044336A (ko) * | 2002-11-12 | 2004-05-28 | 마쯔시다덴기산교 가부시키가이샤 | 특이결합반응 측정방법과 이에 이용하는 시약키트 및특이결합반응 측정장치 |
| US7781172B2 (en) | 2003-11-21 | 2010-08-24 | Kimberly-Clark Worldwide, Inc. | Method for extending the dynamic detection range of assay devices |
| US7247500B2 (en) | 2002-12-19 | 2007-07-24 | Kimberly-Clark Worldwide, Inc. | Reduction of the hook effect in membrane-based assay devices |
| US7851209B2 (en) | 2003-04-03 | 2010-12-14 | Kimberly-Clark Worldwide, Inc. | Reduction of the hook effect in assay devices |
| US20040197819A1 (en) | 2003-04-03 | 2004-10-07 | Kimberly-Clark Worldwide, Inc. | Assay devices that utilize hollow particles |
| US20050112703A1 (en) | 2003-11-21 | 2005-05-26 | Kimberly-Clark Worldwide, Inc. | Membrane-based lateral flow assay devices that utilize phosphorescent detection |
| US7943395B2 (en) | 2003-11-21 | 2011-05-17 | Kimberly-Clark Worldwide, Inc. | Extension of the dynamic detection range of assay devices |
| US7713748B2 (en) | 2003-11-21 | 2010-05-11 | Kimberly-Clark Worldwide, Inc. | Method of reducing the sensitivity of assay devices |
| US20050124008A1 (en) * | 2003-12-05 | 2005-06-09 | Kauvar Lawrence M. | Homogeneous competition assays |
| US7943089B2 (en) | 2003-12-19 | 2011-05-17 | Kimberly-Clark Worldwide, Inc. | Laminated assay devices |
| US7815854B2 (en) | 2004-04-30 | 2010-10-19 | Kimberly-Clark Worldwide, Inc. | Electroluminescent illumination source for optical detection systems |
| US7796266B2 (en) | 2004-04-30 | 2010-09-14 | Kimberly-Clark Worldwide, Inc. | Optical detection system using electromagnetic radiation to detect presence or quantity of analyte |
| JP4730304B2 (ja) * | 2004-05-20 | 2011-07-20 | 和光純薬工業株式会社 | ヒアルロン酸バインディングプロテインを用いたヒアルロン酸の測定方法 |
| US7521226B2 (en) | 2004-06-30 | 2009-04-21 | Kimberly-Clark Worldwide, Inc. | One-step enzymatic and amine detection technique |
| US7170035B2 (en) * | 2004-08-04 | 2007-01-30 | Hottboxx Llc | Heated construction box |
| ITMI20041800A1 (it) * | 2004-09-21 | 2004-12-21 | Univ Degli Studi Milano | Netodo di misurazione di interazioni molecolari con lls |
| EP1952158A2 (en) * | 2005-11-12 | 2008-08-06 | Platform Diagnostics Limited | Agglutination assay |
| PL2244086T3 (pl) * | 2006-04-08 | 2021-11-22 | F. Hoffmann-La Roche Ag | Analiza danych optycznych za pomocą histogramów |
| US7776618B2 (en) * | 2007-03-01 | 2010-08-17 | Church & Dwight Co., Inc. | Diagnostic detection device |
| ITPO20070023A1 (it) | 2007-09-26 | 2009-03-27 | Hospitex Diagnostics S R L | Metodo e dispositivo per l'analisi multipla ad alta sensibilita di campioni biologici |
| EP2513650A4 (en) * | 2009-12-17 | 2013-12-18 | Abay Sa | NEW ANALYTE DETECTION ASSAYS IN SAMPLES AND KITS AND COMPOSITIONS RELATING THERETO |
| JP2013531787A (ja) | 2010-05-25 | 2013-08-08 | アリックス インコーポレイテッド | 粒子の運動度および／または細胞の分散を求めるためのホログラフィック変動顕微鏡装置および方法 |
| US9618523B2 (en) * | 2013-02-28 | 2017-04-11 | Siemens Healthcare Diagnostics Inc. | Methods and reagents for determining isomeric analytes |
| US20150046862A1 (en) * | 2013-08-11 | 2015-02-12 | Silicon Graphics International Corp. | Modifying binning operations |
| US11144184B2 (en) | 2014-01-23 | 2021-10-12 | Mineset, Inc. | Selection thresholds in a visualization interface |
| EP3279635B1 (en) * | 2016-08-04 | 2022-06-01 | Malvern Panalytical Limited | Method, processor and machine-readable, non-transient storage medium for characterising particles suspended in a fluid dispersant |
| JP6760508B2 (ja) * | 2017-08-10 | 2020-09-23 | 株式会社Ｊｖｃケンウッド | 分析方法及び分析装置 |
| CN113358882A (zh) * | 2020-03-04 | 2021-09-07 | 上海鑫谱生物科技有限公司 | 一种光谱分析方法和装置 |
| CN111398577A (zh) * | 2020-06-08 | 2020-07-10 | 南京颐兰贝生物科技有限责任公司 | 一种定量免疫检测方法 |
| EP4409288A1 (en) * | 2021-10-01 | 2024-08-07 | PNP Research LLC | Optical background suppression in binding assays that employ polymeric microspheres |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3873204A (en) * | 1970-01-14 | 1975-03-25 | Bio Physics Systems Inc | Optical extinction photoanalysis apparatus for small particles |
| NL7807532A (nl) * | 1978-07-13 | 1980-01-15 | Akzo Nv | Metaal-immunotest. |
| DE2918342A1 (de) * | 1979-05-07 | 1980-11-20 | Behringwerke Ag | Latex-reagenz |
| JPS57175957A (en) * | 1981-04-24 | 1982-10-29 | Chugai Pharmaceut Co Ltd | Measuring method and device for antigen- antibody reaction |
| US4480042A (en) * | 1981-10-28 | 1984-10-30 | E. I. Du Pont De Nemours And Company | Covalently bonded high refractive index particle reagents and their use in light scattering immunoassays |
| US4581334A (en) * | 1983-04-25 | 1986-04-08 | Ortho Diagnostics Systems, Inc. | Simultaneous detection of leukocyte phagocytic and killing ability |
| AU7385387A (en) * | 1986-06-09 | 1987-12-10 | Ortho Diagnostic Systems Inc. | Immunoassay using detection of colloidal gold |
| US5017009A (en) * | 1986-06-26 | 1991-05-21 | Ortho Diagnostic Systems, Inc. | Scattered total internal reflectance immunoassay system |
| US5252493A (en) * | 1986-09-22 | 1993-10-12 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus therefor |
| US4853335A (en) * | 1987-09-28 | 1989-08-01 | Olsen Duane A | Colloidal gold particle concentration immunoassay |
| GB8800702D0 (en) * | 1988-01-13 | 1988-02-10 | Nycomed As | Test method & reagent kit therefor |
| US5162863A (en) * | 1988-02-15 | 1992-11-10 | Canon Kabushiki Kaisha | Method and apparatus for inspecting a specimen by optical detection of antibody/antigen sensitized carriers |
| NO164622C (no) * | 1988-05-11 | 1990-10-24 | Tore Lindmo | Binaer immunometrisk partikkelbasert metode for maaling av spesifikke serum-antigener ved hjelp av vaeskestroemsmikrofotometri og et ferdigpreparert maaloppsett derav. |
| US5079172A (en) * | 1988-11-04 | 1992-01-07 | Board Of Trustees Operating Michigan State University | Method for detecting the presence of antibodies using gold-labeled antibodies and test kit |
| CA2026409C (en) * | 1989-09-29 | 2004-11-09 | Ernest G. Schutt | Method of producing a reagent containing a narrow distribution of colloidal particles of a selected size and the use thereof |
| US5286452A (en) * | 1991-05-20 | 1994-02-15 | Sienna Biotech, Inc. | Simultaneous multiple assays |
-
1993
- 1993-06-15 TW TW082104758A patent/TW239881B/zh active
- 1993-12-21 CN CN93119931A patent/CN1061142C/zh not_active Expired - Fee Related
- 1993-12-22 EP EP94904875A patent/EP0676045A4/en not_active Withdrawn
- 1993-12-22 CA CA002151060A patent/CA2151060A1/en not_active Abandoned
- 1993-12-22 JP JP6515382A patent/JPH08505231A/ja active Pending
- 1993-12-22 WO PCT/US1993/012428 patent/WO1994015193A1/en not_active Application Discontinuation
- 1993-12-22 KR KR1019950702661A patent/KR0171452B1/ko not_active Expired - Fee Related
- 1993-12-22 AU AU58734/94A patent/AU670489B2/en not_active Ceased
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1994
- 1994-08-05 US US08/286,778 patent/US5589401A/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011095265A (ja) * | 1999-01-23 | 2011-05-12 | Minerva Biotechnologies Corp | コロイド固定化種と非コロイド構造上の種との相互作用 |
| JP2002540383A (ja) * | 1999-01-25 | 2002-11-26 | ミナーヴァ・バイオテクノロジーズ・コーポレーション | 神経変性疾患における異常型タンパク質凝集の迅速かつ高感度の検出 |
| JP2012230124A (ja) * | 1999-01-25 | 2012-11-22 | Minerva Biotechnologies Corp | 神経変性疾患における異常型タンパク質凝集の迅速かつ高感度の検出 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU670489B2 (en) | 1996-07-18 |
| CA2151060A1 (en) | 1994-07-07 |
| WO1994015193A1 (en) | 1994-07-07 |
| EP0676045A1 (en) | 1995-10-11 |
| EP0676045A4 (en) | 1998-02-04 |
| CN1092866A (zh) | 1994-09-28 |
| TW239881B (en, 2012) | 1995-02-01 |
| AU5873494A (en) | 1994-07-19 |
| US5589401A (en) | 1996-12-31 |
| KR0171452B1 (ko) | 1999-05-01 |
| CN1061142C (zh) | 2001-01-24 |
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