JPH07108232B2 - ペプチド又は蛋白質の製造方法 - Google Patents

ペプチド又は蛋白質の製造方法

Info

Publication number
JPH07108232B2
JPH07108232B2 JP2271880A JP27188090A JPH07108232B2 JP H07108232 B2 JPH07108232 B2 JP H07108232B2 JP 2271880 A JP2271880 A JP 2271880A JP 27188090 A JP27188090 A JP 27188090A JP H07108232 B2 JPH07108232 B2 JP H07108232B2
Authority
JP
Japan
Prior art keywords
protein
peptide
protease
arg
dipeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2271880A
Other languages
English (en)
Japanese (ja)
Other versions
JPH04148694A (ja
Inventor
浩明 山本
邦彦 山下
Original Assignee
エム・ディ・リサーチ株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by エム・ディ・リサーチ株式会社 filed Critical エム・ディ・リサーチ株式会社
Priority to JP2271880A priority Critical patent/JPH07108232B2/ja
Priority to EP91904654A priority patent/EP0591524A1/en
Priority to CA002070781A priority patent/CA2070781A1/en
Priority to PCT/JP1991/000239 priority patent/WO1992006211A1/ja
Publication of JPH04148694A publication Critical patent/JPH04148694A/ja
Priority to US08/243,082 priority patent/US5506120A/en
Publication of JPH07108232B2 publication Critical patent/JPH07108232B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/57563—Vasoactive intestinal peptide [VIP]; Related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Vascular Medicine (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2271880A 1990-10-09 1990-10-09 ペプチド又は蛋白質の製造方法 Expired - Lifetime JPH07108232B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2271880A JPH07108232B2 (ja) 1990-10-09 1990-10-09 ペプチド又は蛋白質の製造方法
EP91904654A EP0591524A1 (en) 1990-10-09 1991-02-25 Process for producing peptide or protein
CA002070781A CA2070781A1 (en) 1990-10-09 1991-02-25 Method of producing peptides or proteins
PCT/JP1991/000239 WO1992006211A1 (en) 1990-10-09 1991-02-25 Process for producing peptide or protein
US08/243,082 US5506120A (en) 1990-10-09 1994-05-16 Method of producing peptides or proteins as fusion proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2271880A JPH07108232B2 (ja) 1990-10-09 1990-10-09 ペプチド又は蛋白質の製造方法

Publications (2)

Publication Number Publication Date
JPH04148694A JPH04148694A (ja) 1992-05-21
JPH07108232B2 true JPH07108232B2 (ja) 1995-11-22

Family

ID=17506194

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2271880A Expired - Lifetime JPH07108232B2 (ja) 1990-10-09 1990-10-09 ペプチド又は蛋白質の製造方法

Country Status (5)

Country Link
US (1) US5506120A (enExample)
EP (1) EP0591524A1 (enExample)
JP (1) JPH07108232B2 (enExample)
CA (1) CA2070781A1 (enExample)
WO (1) WO1992006211A1 (enExample)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
ATE220105T1 (de) * 1991-04-01 2002-07-15 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
FR2701953B1 (fr) * 1993-02-22 1995-05-24 Centre Nat Rech Scient Protéine de fusion multi-VIP et procédé de préparation de VIP recombinant.
US6316004B1 (en) * 1993-06-22 2001-11-13 T. Tikhonenko Chimeric somatostatin containing protein and encoding DNA, plasmids of expression, method for preparing chimeric protein, strain-producers, immunogenic composition, method for increasing the productivity of farm animals
US5888775A (en) * 1994-04-29 1999-03-30 Dade International Inc Peptide synthesis and purification by fusion to penI protein or precipitation effective portion thereof
US6037145A (en) * 1994-09-07 2000-03-14 Suntory Limited Process for production of protein
ATE247129T1 (de) * 1995-04-11 2003-08-15 Merck & Co Inc Biologisches verfahren zur herstellung von verbindungen auf der basis der dipeptiden
WO1996034958A1 (en) * 1995-05-03 1996-11-07 Biostar Inc. Vasoactive intestinal peptide
US6037321A (en) * 1995-05-03 2000-03-14 Biostar Inc. Fusion proteins comprising vasoactive intestinal peptide or PACAP
AU6290496A (en) * 1995-06-27 1997-01-30 Igen International, Inc. High-level expression and efficient recovery of ubiquitin fusion proteins from escherichia coli
GB9513967D0 (en) * 1995-07-08 1995-09-06 Univ Leicester Insulin
US20080286228A1 (en) * 1995-10-20 2008-11-20 Tarantolo Stefano R Compositions and methods for enhancing immune responses mediated by antigen-presenting cells
DE69637571D1 (de) * 1995-10-20 2008-08-07 Univ Nebraska Zusammensetzung und verfahren zur verstärkung der immunantwort antigen-präsentierender zellen
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
US5935824A (en) * 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
CA2198966C (en) * 1996-03-04 2011-06-21 Yuji Suzuki Method for cleaving chimeric protein using processing enzyme
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
GB9716790D0 (en) 1997-08-07 1997-10-15 Creative Peptides Sweden Ab Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
KR100368073B1 (ko) * 1999-03-09 2003-01-24 한국생명공학연구원 융합파트너를 이용한 재조합 단백질의 제조방법
EP2213743A1 (en) 2000-04-12 2010-08-04 Human Genome Sciences, Inc. Albumin fusion proteins
US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20080194481A1 (en) 2001-12-21 2008-08-14 Human Genome Sciences, Inc. Albumin Fusion Proteins
AU2002364586A1 (en) 2001-12-21 2003-07-30 Delta Biotechnology Limited Albumin fusion proteins
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins
WO2003059934A2 (en) 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
CA2482995C (en) * 2002-04-22 2013-01-29 Dow Global Technologies Inc. Low-cost production of peptides
EP1534835B1 (en) * 2002-06-29 2010-09-08 Korea Research Institute of Bioscience and Biotechnology Hansenula polymorpha yapsin deficient mutant strain and process for the preparation of recombinant proteins using the same
WO2004060403A2 (en) * 2003-01-06 2004-07-22 Angiochem Inc. Aprotinin and anglos as carriers across the blood-brain barrier
US9657323B2 (en) * 2003-09-30 2017-05-23 Daiichi Sankyo Company, Limited Polypeptide cleavage method using OmpT protease variant
HUE027902T2 (en) 2004-02-09 2016-11-28 Human Genome Sciences Inc Corp Service Company Albumin fusion proteins
RU2369404C2 (ru) * 2004-02-09 2009-10-10 Хьюман Дженом Сайенсиз, Инк. Слитые белки альбумина
US20130172274A1 (en) 2005-12-20 2013-07-04 Duke University Methods and compositions for delivering active agents with enhanced pharmacological properties
WO2007146038A2 (en) 2006-06-07 2007-12-21 Human Genome Sciences, Inc. Albumin fusion proteins
US9365634B2 (en) * 2007-05-29 2016-06-14 Angiochem Inc. Aprotinin-like polypeptides for delivering agents conjugated thereto to tissues
CN102131516B (zh) 2008-06-27 2016-03-16 杜克大学 包含弹性蛋白样肽的治疗剂
CA2740316A1 (en) 2008-10-15 2010-04-22 Angiochem Inc. Conjugates of glp-1 agonists and uses thereof
US9029505B2 (en) * 2009-08-14 2015-05-12 Phasebio Pharmaceuticals, Inc. Modified vasoactive intestinal peptides
CA2873553C (en) 2011-06-06 2020-01-28 Phasebio Pharmaceuticals, Inc. Use of modified vasoactive intestinal peptides in the treatment of hypertension
US11052132B2 (en) 2014-05-08 2021-07-06 Phasebio Pharmaceuticals, Inc. Methods and compositions for treating cystic fibrosis
CN114652817B (zh) 2015-02-09 2026-04-17 费斯生物制药公司 用于治疗肌肉疾病和病症的方法和组合物
EP3307326B9 (en) 2015-06-15 2021-02-24 Angiochem Inc. Methods for the treatment of leptomeningeal carcinomatosis
CA3057252A1 (en) 2017-03-20 2018-09-27 Lupin Limited Expression and large-scale production of peptides

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO158880C (no) * 1977-11-08 1988-11-09 Genentech Inc Fremgangsmaate for fremstilling av et spesifikt pattedyrhormon.
CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
US4703004A (en) * 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
CA1213537A (en) * 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Polypeptide expression method
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
CA1260858A (en) * 1985-03-28 1989-09-26 Lawrence S. Cousens Expression using fused genes providing for protein product
JP2862870B2 (ja) * 1986-09-12 1999-03-03 協和醗酵工業株式会社 新規ペプチド
DE3851117T2 (de) * 1987-10-16 1995-03-30 Genencor Int Sequenzspezifische saure E.coli-Protease.
JPH01191683A (ja) * 1988-01-27 1989-08-01 M D Res Kk 新規なプロテアーゼ
JPH0249585A (ja) * 1988-08-12 1990-02-19 M D Res Kk 新規なプロテアーゼ
EP0336056B1 (en) * 1988-01-27 1995-04-05 M & D RESEARCH CO., LTD. Protease
JP2643968B2 (ja) * 1988-02-03 1997-08-25 サントリー株式会社 Ｋｅｘ２エンドプロテアーゼ及びその製造方法
JPH02271880A (ja) * 1989-04-13 1990-11-06 Suzuki Gakki Seisakusho:Kk 呼吸器官鍛練器

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Ｊ．Ｂａｃｔｅｒｉｏｌ．，Ｖｏｌ．１７０，Ｐ．５６２５−５６３２（１９８８）
Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．，Ｖｏｌ．２６２，Ｎｏ．２０，Ｐ．９６１５−９６２０（１９８７）

Also Published As

Publication number Publication date
WO1992006211A1 (en) 1992-04-16
JPH04148694A (ja) 1992-05-21
EP0591524A4 (enExample) 1994-08-03
EP0591524A1 (en) 1994-04-13
CA2070781A1 (en) 1992-04-10
US5506120A (en) 1996-04-09

Similar Documents

Publication Publication Date Title
JPH07108232B2 (ja) ペプチド又は蛋白質の製造方法
KR960011919B1 (ko) IgA 단백질 분해효소를 이용한 재조합 단백질의 효소적 절단방법
US5707826A (en) Enzymatic method for modification of recombinant polypeptides
US4543329A (en) Process for the specific cleavage of protein sequences from proteins
CA1200773A (en) Expression linkers
CA2237296C (en) Process for the preparation of peptides by way of streptavidin fusion proteins
NZ338004A (en) Process for producing peptide with glucagon like peptide-1 (GLP-1) activity by incorporating a protective and helper component to facilitate purification
JP3169131B2 (ja) 外在的なペプチド配列を用いた処理による生物学的に活性なポリペプチドの製造方法およびそのポリペプチド
WO1986007380A1 (fr) Procede de preparation de polypeptides
AU777408B2 (en) Production of pancreatic procarboxy-peptidase B, isoforms and muteins thereof, and their use
RU2120475C1 (ru) Способ получения представляющего интерес полипептида, гибридная днк (варианты), слитый белок (варианты)
RU2108386C1 (ru) Рекомбинантный гранулоцит-колониестимулирующий фактор (g - csf) без дополнительного остатка метионина на n-конце
JPH04148686A (ja) 形質転換体およびペプチド前駆体タンパク質の製造方法
JPH04179495A (ja) 融合蛋白質、およびペプチド又は蛋白質の製造方法
Koller et al. The tendamistat expression-secretion system: synthesis of proinsulin fusion proteins with Streptomyces lividans
Itoh et al. Maturation of an NH2-terminally extended thermostable α-amylase in Bacillus subtilis: a possible mechanism examined by in vitro experiments
JPH01181796A (ja) 組換えｄｎａ法を用いたポリペプチドの製造法
JPH0740924B2 (ja) 形質転換体および融合蛋白質の製造方法
AG THE TENDAMISTAT EXPRESSION-SECRETION SYSTEM: SYNTHESIS OF PROINSULIN FUSION PROTEINS YITH STREPTOMYCES LIVIDANS
JPS6244920B2 (enExample)