JPH026023B2 - - Google Patents
Info
- Publication number
- JPH026023B2 JPH026023B2 JP55083630A JP8363080A JPH026023B2 JP H026023 B2 JPH026023 B2 JP H026023B2 JP 55083630 A JP55083630 A JP 55083630A JP 8363080 A JP8363080 A JP 8363080A JP H026023 B2 JPH026023 B2 JP H026023B2
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- antigen
- reaction
- fraction
- measured
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000006243 chemical reaction Methods 0.000 claims description 50
- 239000000427 antigen Substances 0.000 claims description 47
- 102000036639 antigens Human genes 0.000 claims description 47
- 108091007433 antigens Proteins 0.000 claims description 47
- 238000000034 method Methods 0.000 claims description 29
- 239000000470 constituent Substances 0.000 claims description 20
- 108060003951 Immunoglobulin Proteins 0.000 claims description 19
- 102000018358 immunoglobulin Human genes 0.000 claims description 19
- 239000003381 stabilizer Substances 0.000 claims description 18
- 230000008105 immune reaction Effects 0.000 claims description 14
- 239000000376 reactant Substances 0.000 claims description 11
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 239000012429 reaction media Substances 0.000 claims description 6
- 102000008186 Collagen Human genes 0.000 claims description 4
- 108010035532 Collagen Proteins 0.000 claims description 4
- 108010058846 Ovalbumin Proteins 0.000 claims description 4
- 108010071390 Serum Albumin Proteins 0.000 claims description 4
- 102000007562 Serum Albumin Human genes 0.000 claims description 4
- 229920001436 collagen Polymers 0.000 claims description 4
- 238000000691 measurement method Methods 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 2
- 229940072221 immunoglobulins Drugs 0.000 claims 1
- 239000004816 latex Substances 0.000 description 15
- 229920000126 latex Polymers 0.000 description 15
- 239000000872 buffer Substances 0.000 description 14
- 239000003153 chemical reaction reagent Substances 0.000 description 13
- 239000002245 particle Substances 0.000 description 9
- 229940027941 immunoglobulin g Drugs 0.000 description 8
- 239000012736 aqueous medium Substances 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- 230000004520 agglutination Effects 0.000 description 6
- 239000012491 analyte Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 206010029719 Nonspecific reaction Diseases 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 239000002504 physiological saline solution Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 239000000969 carrier Substances 0.000 description 4
- 238000003127 radioimmunoassay Methods 0.000 description 4
- 230000009257 reactivity Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000700199 Cavia porcellus Species 0.000 description 3
- 102000036675 Myoglobin Human genes 0.000 description 3
- 108010062374 Myoglobin Proteins 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000011859 microparticle Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 238000004879 turbidimetry Methods 0.000 description 3
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 108090000526 Papain Proteins 0.000 description 2
- 102000057297 Pepsin A Human genes 0.000 description 2
- 108090000284 Pepsin A Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 206010070834 Sensitisation Diseases 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 238000000149 argon plasma sintering Methods 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 2
- 239000010419 fine particle Substances 0.000 description 2
- 229940106780 human fibrinogen Drugs 0.000 description 2
- 230000028993 immune response Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 229940099472 immunoglobulin a Drugs 0.000 description 2
- 229940049920 malate Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229940055729 papain Drugs 0.000 description 2
- 235000019834 papain Nutrition 0.000 description 2
- 229940111202 pepsin Drugs 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000008313 sensitization Effects 0.000 description 2
- 230000001235 sensitizing effect Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 241000606701 Rickettsia Species 0.000 description 1
- 241000607715 Serratia marcescens Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- XECAHXYUAAWDEL-UHFFFAOYSA-N acrylonitrile butadiene styrene Chemical compound C=CC=C.C=CC#N.C=CC1=CC=CC=C1 XECAHXYUAAWDEL-UHFFFAOYSA-N 0.000 description 1
- 229920000122 acrylonitrile butadiene styrene Polymers 0.000 description 1
- 239000004676 acrylonitrile butadiene styrene Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052809 inorganic oxide Inorganic materials 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 206010039073 rheumatoid arthritis Diseases 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 229920003048 styrene butadiene rubber Polymers 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8363080A JPS579723A (en) | 1980-06-20 | 1980-06-20 | Stabilizing agent for immunological reaction and measuring method of antigen-antibody reaction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8363080A JPS579723A (en) | 1980-06-20 | 1980-06-20 | Stabilizing agent for immunological reaction and measuring method of antigen-antibody reaction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS579723A JPS579723A (en) | 1982-01-19 |
| JPH026023B2 true JPH026023B2 (fr) | 1990-02-07 |
Family
ID=13807780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8363080A Granted JPS579723A (en) | 1980-06-20 | 1980-06-20 | Stabilizing agent for immunological reaction and measuring method of antigen-antibody reaction |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS579723A (fr) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3266967D1 (en) * | 1982-01-05 | 1985-11-21 | Int Inst Cellular Molecul Path | Method of immunoassay |
| DE3206729A1 (de) * | 1982-02-25 | 1983-09-01 | Behringwerke Ag, 3550 Marburg | Immunologisches agglutinationsverfahren |
| JPS5992353A (ja) * | 1982-11-19 | 1984-05-28 | Shinotesuto Kenkyusho:Kk | 不溶性担体粒子を用いる凝集反応測定方法 |
| JPH0634013B2 (ja) * | 1985-03-25 | 1994-05-02 | 栄治 石川 | 高感度免疫測定法 |
| JPH0778025B2 (ja) * | 1990-03-20 | 1995-08-23 | 日本赤十字社 | 免疫グロブリンｇの製造方法 |
| DE4439452A1 (de) * | 1994-11-04 | 1996-05-09 | Boehringer Mannheim Gmbh | Antikörperklassenspezifisches Entstörungsreagenz |
| JPH0968531A (ja) * | 1995-08-31 | 1997-03-11 | Sanyo Chem Ind Ltd | 免疫測定法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5512419A (en) * | 1978-07-13 | 1980-01-29 | Teikoku Hormone Mfg Co Ltd | Immunological measurement and reagent therefor |
-
1980
- 1980-06-20 JP JP8363080A patent/JPS579723A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5512419A (en) * | 1978-07-13 | 1980-01-29 | Teikoku Hormone Mfg Co Ltd | Immunological measurement and reagent therefor |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS579723A (en) | 1982-01-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU614109B2 (en) | Test method and reagent kit therefor | |
| US7759074B2 (en) | Immunological latex turbidimetry method and reagent therefor | |
| EP0163312A2 (fr) | Immunoessai | |
| CA1195925A (fr) | Methode de dosage immunologique et reactifs utilises a cet effet | |
| WO2002039114A2 (fr) | Dosage, reatifs et necessaires destines a detecter ou determiner la concentration des analytes | |
| US4239743A (en) | Carrier-bound immunoglobulin fission product and its use in immunologic analyses | |
| NL8003294A (nl) | Werkwijze voor het bepalen van een antigeen, antilichaam of antigeenantilichaamcomplex alsmede daarvoor bestemde meetkit. | |
| JP3623657B2 (ja) | 非特異反応抑制剤、免疫測定試薬及び免疫測定方法 | |
| US5043289A (en) | Method and device for assaying immunologically reactive substances of clinical interest | |
| JP3899029B2 (ja) | 免疫学的分析方法 | |
| JPH026023B2 (fr) | ||
| EP0064275B1 (fr) | Réactif immunochimique | |
| AU657595B2 (en) | A method for the determination of antigens or antibodies in the presence of an immune complex | |
| JPH1123573A (ja) | 免疫学的測定方法 | |
| JPH0712818A (ja) | 免疫学的検出方法 | |
| JP3048306B2 (ja) | フィブリン分解産物及び／又はフィブリノーゲン分解産物測定試薬及びその定量方法 | |
| JP3328053B2 (ja) | 免疫凝集反応による抗体又は抗原の濃度定量法 | |
| JPH09304386A (ja) | 免疫診断薬の製造方法および得られた免疫診断薬 | |
| JP3618797B2 (ja) | 免疫測定法 | |
| JP2000258419A (ja) | 免疫測定試薬及び免疫測定法 | |
| JPS63200064A (ja) | 抗原抗体反応の測定法 | |
| JPH0560757A (ja) | 抗原の定量法及び抗原定量用免疫試薬 | |
| JP3692055B2 (ja) | 抗原抗体反応の判定方法 | |
| JPH10253629A (ja) | 免疫測定試薬製造法 | |
| JPH0833396B2 (ja) | 免疫測定用試薬およびそれを用いた免疫測定法 |