JPH0159873B2 - - Google Patents

Info

Publication number
JPH0159873B2
JPH0159873B2 JP59202199A JP20219984A JPH0159873B2 JP H0159873 B2 JPH0159873 B2 JP H0159873B2 JP 59202199 A JP59202199 A JP 59202199A JP 20219984 A JP20219984 A JP 20219984A JP H0159873 B2 JPH0159873 B2 JP H0159873B2
Authority
JP
Japan
Prior art keywords
enzyme
buffer
dna
culture
restriction enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
JP59202199A
Other languages
English (en)
Japanese (ja)
Other versions
JPS6178382A (ja
Inventor
Akira Oohayashi
Shinji Hiraoka
Keiko Kita
Hiroshi Nakajima
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takara Shuzo Co Ltd
Original Assignee
Takara Shuzo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Shuzo Co Ltd filed Critical Takara Shuzo Co Ltd
Priority to JP59202199A priority Critical patent/JPS6178382A/ja
Priority to DE19853530218 priority patent/DE3530218A1/de
Priority to GB08521891A priority patent/GB2164946B/en
Priority to US06/777,674 priority patent/US4668631A/en
Publication of JPS6178382A publication Critical patent/JPS6178382A/ja
Publication of JPH0159873B2 publication Critical patent/JPH0159873B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
JP59202199A 1984-09-27 1984-09-27 制限酵素の製造法 Granted JPS6178382A (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP59202199A JPS6178382A (ja) 1984-09-27 1984-09-27 制限酵素の製造法
DE19853530218 DE3530218A1 (de) 1984-09-27 1985-08-23 Verfahren zur herstellung einer restriktionsendonuklease
GB08521891A GB2164946B (en) 1984-09-27 1985-09-03 New restriction enzyme and process for producing the same
US06/777,674 US4668631A (en) 1984-09-27 1985-09-19 Process for producing a restriction enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP59202199A JPS6178382A (ja) 1984-09-27 1984-09-27 制限酵素の製造法

Publications (2)

Publication Number Publication Date
JPS6178382A JPS6178382A (ja) 1986-04-21
JPH0159873B2 true JPH0159873B2 (enFirst) 1989-12-20

Family

ID=16453603

Family Applications (1)

Application Number Title Priority Date Filing Date
JP59202199A Granted JPS6178382A (ja) 1984-09-27 1984-09-27 制限酵素の製造法

Country Status (4)

Country Link
US (1) US4668631A (enFirst)
JP (1) JPS6178382A (enFirst)
DE (1) DE3530218A1 (enFirst)
GB (1) GB2164946B (enFirst)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4975376A (en) * 1988-04-02 1990-12-04 Boehringer Mannheim Gmbh Class II restriction endonuclease KspI and a process for obtaining it
JPH02255086A (ja) * 1989-03-29 1990-10-15 Nisshin Seito Kk 新規制限酵素及びその製造法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56140888A (en) * 1980-04-02 1981-11-04 Yakult Honsha Co Ltd Preparation of limiting enzyme
JPS5860986A (ja) * 1981-10-05 1983-04-11 Yakult Honsha Co Ltd 新規制限酵素及びその製造法

Also Published As

Publication number Publication date
DE3530218A1 (de) 1986-04-03
GB2164946A (en) 1986-04-03
DE3530218C2 (enFirst) 1988-03-31
GB2164946B (en) 1988-11-09
JPS6178382A (ja) 1986-04-21
US4668631A (en) 1987-05-26
GB8521891D0 (en) 1985-10-09

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