JP7833394B2 - Ｒｎａウイルスの検出方法 - Google Patents

Ｒｎａウイルスの検出方法

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Publication number
JP7833394B2
JP7833394B2 JP2022518120A JP2022518120A JP7833394B2 JP 7833394 B2 JP7833394 B2 JP 7833394B2 JP 2022518120 A JP2022518120 A JP 2022518120A JP 2022518120 A JP2022518120 A JP 2022518120A JP 7833394 B2 JP7833394 B2 JP 7833394B2
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nucleic acid
polypeptide
biological sample
activity
swabs
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Japanese (ja)
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JPWO2021221109A1 (https=
Inventor
隆司 上森
武宏 相良
美和 秋友
憲介 齋藤
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Takara Bio Inc
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Takara Bio Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2022518120A 2020-04-30 2021-04-28 Ｒｎａウイルスの検出方法 Active JP7833394B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2020080247 2020-04-30
JP2020080247 2020-04-30
JP2020107940 2020-06-23
JP2020107940 2020-06-23
PCT/JP2021/016998 WO2021221109A1 (ja) 2020-04-30 2021-04-28 Ｒｎａウイルスの検出方法

Publications (2)

Publication Number Publication Date
JPWO2021221109A1 JPWO2021221109A1 (https=) 2021-11-04
JP7833394B2 true JP7833394B2 (ja) 2026-03-19

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US (1) US20230160023A1 (https=)
EP (1) EP4144863A4 (https=)
JP (1) JP7833394B2 (https=)
KR (1) KR20230005897A (https=)
CN (1) CN115667556A (https=)
WO (1) WO2021221109A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023127774A1 (ja) * 2021-12-28 2023-07-06 株式会社ダナフォーム 遺伝子包含体の検出方法及び検出用キット
CN114675017B (zh) * 2022-03-25 2025-08-05 艾康生物技术(杭州)有限公司 一种生物样本提取液及其应用和使用方法
US20240381431A1 (en) * 2023-05-12 2024-11-14 Qualcomm Incorporated Determination of physical random access channel repetition numbers and associated selection criteria

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008135197A1 (en) 2007-05-03 2008-11-13 Roche Diagnostics Gmbh Improved lysis and reverse transcription for mrna quantification
WO2013002354A1 (ja) 2011-06-29 2013-01-03 株式会社ダナフォーム 生体試料の前処理方法、ｒｎａの検出方法及び前処理キット
CN104928402A (zh) 2015-06-15 2015-09-23 菲鹏生物股份有限公司 荧光定量pcr检测核酸分子的方法
JP2017209036A (ja) 2016-05-24 2017-11-30 東洋紡株式会社 改良されたウイルス検出方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0646900A (ja) 1992-07-29 1994-02-22 Tonen Corp 単一容器内での逆転写酵素介在二段階遺伝子増幅反応によるｒｎａ遺伝子の増幅検出方法
CA2161337A1 (en) * 1993-05-06 1994-11-24 Denis R. Henrard Direct lysis buffer and the detection of hiv-1 plasma viremia
EP1476573B1 (en) * 2002-01-28 2010-01-06 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
CN101386893B (zh) * 2008-10-28 2011-07-27 武汉大学 一种检测口蹄疫病毒基因组rna的单细胞实时荧光定量rt-pcr的方法
JP2018000124A (ja) 2016-07-05 2018-01-11 東ソー株式会社 ウイルスからの核酸抽出増幅キット及びそれを用いた抽出増幅方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008135197A1 (en) 2007-05-03 2008-11-13 Roche Diagnostics Gmbh Improved lysis and reverse transcription for mrna quantification
WO2013002354A1 (ja) 2011-06-29 2013-01-03 株式会社ダナフォーム 生体試料の前処理方法、ｒｎａの検出方法及び前処理キット
CN104928402A (zh) 2015-06-15 2015-09-23 菲鹏生物股份有限公司 荧光定量pcr检测核酸分子的方法
JP2017209036A (ja) 2016-05-24 2017-11-30 東洋紡株式会社 改良されたウイルス検出方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HEUNGSUP SUNG、外６名，Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR，Ann Lab Med，2016年，Vol.36,No.5，Page.457-462， doi: 10.3343/alm.2016.36.5.457.，PMID: 27374711

Also Published As

Publication number Publication date
JPWO2021221109A1 (https=) 2021-11-04
KR20230005897A (ko) 2023-01-10
EP4144863A4 (en) 2024-07-31
US20230160023A1 (en) 2023-05-25
CN115667556A (zh) 2023-01-31
WO2021221109A1 (ja) 2021-11-04
EP4144863A1 (en) 2023-03-08

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