CN115667556A - Rna病毒检测方法 - Google Patents

Rna病毒检测方法 Download PDF

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Publication number
CN115667556A
CN115667556A CN202180031798.4A CN202180031798A CN115667556A CN 115667556 A CN115667556 A CN 115667556A CN 202180031798 A CN202180031798 A CN 202180031798A CN 115667556 A CN115667556 A CN 115667556A
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nucleic acid
polypeptide
biological sample
activity
acid amplification
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Pending
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CN202180031798.4A
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Chinese (zh)
Inventor
上森隆司
相良武宏
秋友美和
斋藤宪介
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Takara Bio Inc
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Takara Bio Inc
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
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    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
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    • C12N2320/10—Applications; Uses in screening processes
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202180031798.4A 2020-04-30 2021-04-28 Rna病毒检测方法 Pending CN115667556A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2020-080247 2020-04-30
JP2020080247 2020-04-30
JP2020-107940 2020-06-23
JP2020107940 2020-06-23
PCT/JP2021/016998 WO2021221109A1 (ja) 2020-04-30 2021-04-28 Ｒｎａウイルスの検出方法

Publications (1)

Publication Number Publication Date
CN115667556A true CN115667556A (zh) 2023-01-31

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ID=78331998

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CN202180031798.4A Pending CN115667556A (zh) 2020-04-30 2021-04-28 Rna病毒检测方法

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US (1) US20230160023A1 (https=)
EP (1) EP4144863A4 (https=)
JP (1) JP7833394B2 (https=)
KR (1) KR20230005897A (https=)
CN (1) CN115667556A (https=)
WO (1) WO2021221109A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023127774A1 (ja) * 2021-12-28 2023-07-06 株式会社ダナフォーム 遺伝子包含体の検出方法及び検出用キット
CN114675017B (zh) * 2022-03-25 2025-08-05 艾康生物技术(杭州)有限公司 一种生物样本提取液及其应用和使用方法
US20240381431A1 (en) * 2023-05-12 2024-11-14 Qualcomm Incorporated Determination of physical random access channel repetition numbers and associated selection criteria

Citations (6)

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WO2003064605A2 (en) * 2002-01-28 2003-08-07 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
WO2008135196A1 (en) * 2007-05-03 2008-11-13 Roche Diagnostics Gmbh SINGLE-CELL mRNA QUANTIFICATION WITH REAL-TIME RT-PCR
CN101386893A (zh) * 2008-10-28 2009-03-18 武汉大学 一种检测口蹄疫病毒基因组rna的单细胞实时荧光定量rt-pcr的方法
CN103097531A (zh) * 2011-06-29 2013-05-08 达纳福股份有限公司 生物样本的预处理方法、rna的检测方法及预处理试剂盒
CN104928402A (zh) * 2015-06-15 2015-09-23 菲鹏生物股份有限公司 荧光定量pcr检测核酸分子的方法
JP2017209036A (ja) * 2016-05-24 2017-11-30 東洋紡株式会社 改良されたウイルス検出方法

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JPH0646900A (ja) 1992-07-29 1994-02-22 Tonen Corp 単一容器内での逆転写酵素介在二段階遺伝子増幅反応によるｒｎａ遺伝子の増幅検出方法
CA2161337A1 (en) * 1993-05-06 1994-11-24 Denis R. Henrard Direct lysis buffer and the detection of hiv-1 plasma viremia
JP2018000124A (ja) 2016-07-05 2018-01-11 東ソー株式会社 ウイルスからの核酸抽出増幅キット及びそれを用いた抽出増幅方法

Patent Citations (6)

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WO2003064605A2 (en) * 2002-01-28 2003-08-07 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
WO2008135196A1 (en) * 2007-05-03 2008-11-13 Roche Diagnostics Gmbh SINGLE-CELL mRNA QUANTIFICATION WITH REAL-TIME RT-PCR
CN101386893A (zh) * 2008-10-28 2009-03-18 武汉大学 一种检测口蹄疫病毒基因组rna的单细胞实时荧光定量rt-pcr的方法
CN103097531A (zh) * 2011-06-29 2013-05-08 达纳福股份有限公司 生物样本的预处理方法、rna的检测方法及预处理试剂盒
CN104928402A (zh) * 2015-06-15 2015-09-23 菲鹏生物股份有限公司 荧光定量pcr检测核酸分子的方法
JP2017209036A (ja) * 2016-05-24 2017-11-30 東洋紡株式会社 改良されたウイルス検出方法

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FAN YANG等: "A rapid silica spin column-based method of RNA extraction from fruit trees for RT-PCR detection of viruses", J VIROL METHODS, vol. 24, no. 61, 30 September 2017 (2017-09-30), pages 62 *
HELENA V. CHETVERINA 等: "Simultaneous assay of DNA and RNA targets in the whole blood using novel isolation procedure and molecular colony amplification", ANALYTICAL BIOCHEMISTRY, vol. 334, no. 2, 15 November 2004 (2004-11-15), pages 2 - 3 *
JIANG F. ZHONG等: "Viral RNA extraction for in-the-field analysis", JOURNAL OF VIROLOGICAL METHODS, vol. 144, no. 1, 30 September 2007 (2007-09-30), pages 2 *
N JOTHIKUMAR等: "Development of an RNA extraction protocol for detection of waterborne viruses by reverse transcriptase quantitative PCR (RT-qPCR)", J VIROL METHODS, vol. 169, no. 1, 31 October 2010 (2010-10-31), pages 2 *
李松播等: "聚合酶链式反应技术在药品质量控制领域中的应用", 中国药师, vol. 23, no. 1, 5 February 2020 (2020-02-05), pages 6 *
董关木等: "用PCR方法证实HIV－1感染性病毒颗粒携带有前病毒DNA", 中国病毒学, vol. 9, no. 3, 30 September 1994 (1994-09-30), pages 3 *

Also Published As

Publication number Publication date
JP7833394B2 (ja) 2026-03-19
JPWO2021221109A1 (https=) 2021-11-04
KR20230005897A (ko) 2023-01-10
EP4144863A4 (en) 2024-07-31
US20230160023A1 (en) 2023-05-25
WO2021221109A1 (ja) 2021-11-04
EP4144863A1 (en) 2023-03-08

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