CN101386893A - 一种检测口蹄疫病毒基因组rna的单细胞实时荧光定量rt-pcr的方法 - Google Patents
一种检测口蹄疫病毒基因组rna的单细胞实时荧光定量rt-pcr的方法 Download PDFInfo
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- CN101386893A CN101386893A CNA2008101974119A CN200810197411A CN101386893A CN 101386893 A CN101386893 A CN 101386893A CN A2008101974119 A CNA2008101974119 A CN A2008101974119A CN 200810197411 A CN200810197411 A CN 200810197411A CN 101386893 A CN101386893 A CN 101386893A
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Images
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
编号 | 名称 | 样品类型 | Ct值 | 拷贝数 | 设定值(标品) |
1 | 108 | Standard | 13.09 | 108 | 108 |
2 | 106 | Standard | 19.78 | 106 | 106 |
3 | 104 | Standard | 26.39 | 104 | 104 |
4 | 100 | Standard | 33.04 | 100 | 100 |
5 | PC | Positive Control | 9.45 | 1362783901 | |
6 | SC | Sample | 20.14 | 897603 | |
7 | SC | Sample | 22.33 | 193727 | |
8 | SC | Sample | 23.34 | 83908 | |
9 | SC | Sample | 24.71 | 40801 | |
10 | SC | Sample | 24.95 | 35056 | |
11 | SC | Sample | 25.03 | 33260 | |
12 | SC | Sample | 25.81 | 20283 | |
13 | SC | Sample | 26.07 | 17236 | |
14 | SC | Sample | 26.14 | 16535 | |
15 | SC | Sample | 26.54 | 16055 | |
16 | SC | Sample | 27.07 | 11264 | |
17 | SC | Sample | 27.68 | 9251 | |
18 | SC | Sample | 28.19 | 5598 |
19 | SC | Sample | 28.26 | 5359 | |
20 | SC | Sample | 28.85 | 3707 | |
21 | SC | Sample | 29.15 | 3074 | |
22 | SC | Sample | 29.26 | 2870 | |
23 | SC | Sample | 29.28 | 2834 | |
24 | SC | Sample | 29.39 | 2646 | |
25 | SC | Sample | 29.4 | 2630 | |
26 | SC | Sample | 29.56 | 2380 | |
27 | SC | Sample | 29.57 | 2365 | |
28 | SC | Sample | 29.67 | 2222 | |
29 | SC | Sample | 29.7 | 2181 | |
30 | SC | Sample | 29.77 | 2087 | |
31 | SC | Sample | 29.82 | 2023 | |
32 | SC | Sample | 29.95 | 1865 | |
33 | SC | Sample | 30.29 | 1859 | |
34 | SC | Sample | 30.38 | 1759 | |
35 | SC | Sample | 30.4 | 1215 | |
36 | SC | Sample | 30.62 | 1049 | |
37 | SC | Sample | 30.9 | 1031 | |
38 | SC | Sample | 30.99 | 974 | |
39 | SC | Sample | 31.12 | 703 | |
40 | SC | Sample | 31.86 | 439 | |
41 | SC | Sample | 32.08 | 319 | |
42 | SC | Sample | 33.23 | 183 | |
43 | SC | Sample | 33.83 | 126 | |
44 | SC | Sample | 34.06 | 26 | |
45 | SC | Sample | 34.54 | 14 | |
46 | SC | Sample | 35.44 | 7 | |
47 | SC | Sample | 0 | 0 | |
48 | SC | Sample | 0 | 0 | |
49 | SC | Sample | 0 | 0 |
50 | SC | Sample | 0 | 0 | |
51 | SC | Sample | 0 | 0 | |
52 | SC | Sample | 0 | 0 | |
53 | SC | Sample | 0 | 0 | |
54 | NTC | No Template Control | 无 | 无 | |
55 | NTC | No Template Control | 无 | 无 |
比例 | 个数 | 拷贝数 | |
阴性结果 | 73% | 19 | 0 |
阳性结果 | 27% | 7 | 1194,482,139,45,32,30,12 |
总计 | 100% | 26 |
Claims (3)
Priority Applications (1)
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CN2008101974119A CN101386893B (zh) | 2008-10-28 | 2008-10-28 | 一种检测口蹄疫病毒基因组rna的单细胞实时荧光定量rt-pcr的方法 |
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CN2008101974119A CN101386893B (zh) | 2008-10-28 | 2008-10-28 | 一种检测口蹄疫病毒基因组rna的单细胞实时荧光定量rt-pcr的方法 |
Publications (2)
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CN101386893A true CN101386893A (zh) | 2009-03-18 |
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Country Status (1)
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102260750A (zh) * | 2011-07-20 | 2011-11-30 | 东莞出入境检验检疫局检验检疫综合技术中心 | 用于口蹄疫病毒实时荧光rt-pcr检测的引物和方法 |
CN103937783A (zh) * | 2014-04-29 | 2014-07-23 | 海河流域水环境监测中心 | 一种单克隆微囊藻dna的提取方法 |
CN104388597A (zh) * | 2014-12-11 | 2015-03-04 | 河南省动物疫病预防控制中心 | Fmdv通用型和o型二重荧光定量pcr检测方法 |
CN106957926A (zh) * | 2017-04-14 | 2017-07-18 | 北京出入境检验检疫局检验检疫技术中心 | 一种能同时检测和鉴别古典猪瘟、非洲猪瘟和猪水泡病的检测试剂盒及引物和探针 |
CN115144519A (zh) * | 2022-06-30 | 2022-10-04 | 上海交通大学 | 基于无机纳米颗粒的单细胞样品指纹图谱检测方法和应用 |
-
2008
- 2008-10-28 CN CN2008101974119A patent/CN101386893B/zh not_active Expired - Fee Related
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102260750A (zh) * | 2011-07-20 | 2011-11-30 | 东莞出入境检验检疫局检验检疫综合技术中心 | 用于口蹄疫病毒实时荧光rt-pcr检测的引物和方法 |
CN102260750B (zh) * | 2011-07-20 | 2013-06-05 | 东莞出入境检验检疫局检验检疫综合技术中心 | 用于口蹄疫病毒实时荧光rt-pcr检测的引物和方法 |
CN103937783A (zh) * | 2014-04-29 | 2014-07-23 | 海河流域水环境监测中心 | 一种单克隆微囊藻dna的提取方法 |
CN104388597A (zh) * | 2014-12-11 | 2015-03-04 | 河南省动物疫病预防控制中心 | Fmdv通用型和o型二重荧光定量pcr检测方法 |
CN104388597B (zh) * | 2014-12-11 | 2016-09-28 | 河南省动物疫病预防控制中心 | Fmdv通用型和o型二重荧光定量pcr检测方法 |
CN106957926A (zh) * | 2017-04-14 | 2017-07-18 | 北京出入境检验检疫局检验检疫技术中心 | 一种能同时检测和鉴别古典猪瘟、非洲猪瘟和猪水泡病的检测试剂盒及引物和探针 |
CN115144519A (zh) * | 2022-06-30 | 2022-10-04 | 上海交通大学 | 基于无机纳米颗粒的单细胞样品指纹图谱检测方法和应用 |
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