JP7715045B2 - カルボニル還元酵素、これをコードする核酸、及びこれらを利用した光学活性化合物の製造方法 - Google Patents

カルボニル還元酵素、これをコードする核酸、及びこれらを利用した光学活性化合物の製造方法

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Publication number
JP7715045B2
JP7715045B2 JP2021558467A JP2021558467A JP7715045B2 JP 7715045 B2 JP7715045 B2 JP 7715045B2 JP 2021558467 A JP2021558467 A JP 2021558467A JP 2021558467 A JP2021558467 A JP 2021558467A JP 7715045 B2 JP7715045 B2 JP 7715045B2
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amino acid
acid sequence
seq
carbonyl reductase
microorganism
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JPWO2021100848A1 (https=
JPWO2021100848A5 (https=
Inventor
崇敦 井浦
康方 出来島
剛 阪本
磨理 原
宏敏 平岡
ハラルド グローガー
ジウン チェ
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Ube Corp
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Ube Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C12P17/167—Heterorings having sulfur atoms as ring heteroatoms, e.g. vitamin B1, thiamine nucleus and open chain analogs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01184—Carbonyl reductase (NADPH) (1.1.1.184)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2021558467A 2019-11-22 2020-11-20 カルボニル還元酵素、これをコードする核酸、及びこれらを利用した光学活性化合物の製造方法 Active JP7715045B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019211797 2019-11-22
JP2019211797 2019-11-22
PCT/JP2020/043366 WO2021100848A1 (ja) 2019-11-22 2020-11-20 カルボニル還元酵素、これをコードする核酸、及びこれらを利用した光学活性化合物の製造方法

Publications (3)

Publication Number Publication Date
JPWO2021100848A1 JPWO2021100848A1 (https=) 2021-05-27
JPWO2021100848A5 JPWO2021100848A5 (https=) 2024-05-08
JP7715045B2 true JP7715045B2 (ja) 2025-07-30

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JP2021558467A Active JP7715045B2 (ja) 2019-11-22 2020-11-20 カルボニル還元酵素、これをコードする核酸、及びこれらを利用した光学活性化合物の製造方法

Country Status (5)

Country Link
US (1) US12473578B2 (https=)
EP (1) EP4063512A4 (https=)
JP (1) JP7715045B2 (https=)
CN (1) CN114729374B (https=)
WO (1) WO2021100848A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
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CN115466755B (zh) * 2022-10-24 2025-07-29 常州佳德医药科技有限公司 一种羰基还原酶不对称还原制备(-)-5-氮杂螺[2,4]庚烷-7-醇的方法
CN115820583B (zh) * 2022-12-29 2026-01-30 金达威生物技术(江苏)有限公司 羰基还原酶突变体及其制备方法、应用和(r)-6-羟基-8-氯辛酸乙酯的制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003339387A (ja) 2002-03-19 2003-12-02 Mitsubishi Chemicals Corp 新規カルボニル還元酵素及びこれをコードする遺伝子、ならびにこれらを利用した光学活性アルコールの製造方法
WO2006013801A1 (ja) 2004-08-06 2006-02-09 Kaneka Corporation 新規カルボニル還元酵素、その遺伝子、およびその利用法
WO2011132444A1 (ja) 2010-04-22 2011-10-27 株式会社カネカ 改変型カルボニル還元酵素、その遺伝子、およびそれらを利用した光学活性アルコールの製造方法
WO2015119261A1 (ja) 2014-02-06 2015-08-13 株式会社エーピーアイ コーポレーション ロスバスタチンカルシウム及びその中間体の製造方法
JP2017518744A (ja) 2014-05-09 2017-07-13 凱菜英医藥集團（天津）股▲分▼有限公司Ａｓｙｍｃｈｅｍ Ｌａｂｏｒａｔｏｒｉｅｓ （Ｔｉａｎｊｉｎ） Ｃｏ．， Ｌｔｄ． ジカルボニル還元酵素突然変異体及びその応用

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JPS57183799A (en) 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid
JPH0279866A (ja) 1988-09-16 1990-03-20 Konica Corp カラー画像形成装置
JP2648897B2 (ja) 1991-07-01 1997-09-03 塩野義製薬株式会社 ピリミジン誘導体
JP3481325B2 (ja) 1994-09-06 2003-12-22 宇部興産株式会社 光学活性な３−オキシ−５−オキソ−６−ヘプテン酸誘導体の製法
DE60323215D1 (de) 2002-03-19 2008-10-09 Mitsubishi Chem Corp Neue carbonylreduktase, diese codierendes gen und verfahren zur produktion optisch-aktiver alkohole damit
JP4426437B2 (ja) * 2002-04-30 2010-03-03 株式会社カネカ 新規カルボニル還元酵素、その遺伝子、およびその利用法
JP2005034025A (ja) 2003-07-18 2005-02-10 Mitsubishi Pharma Corp 大腸菌での異種遺伝子発現を促進させるプラスミドベクター、組換え大腸菌、該組換え大腸菌を用いた化学物質製造法及び組換えタンパク質の製造法
JP2007502124A (ja) * 2003-08-11 2007-02-08 コデクシス， インコーポレイテッド 改良ケトレダクターゼポリペプチドおよび関連ポリヌクレオチド
US10676441B2 (en) 2015-08-05 2020-06-09 Api Corporation Method for producing pitavastatin calcium
CN106243205B (zh) * 2016-08-17 2019-08-02 中国科学院植物研究所 一种降解棒曲霉素的蛋白及其编码基因与应用
EP3652328A1 (en) * 2017-07-14 2020-05-20 c-LEcta GmbH Ketoreductases
JP6828109B2 (ja) 2019-09-10 2021-02-10 キヤノン株式会社 画像形成装置
CN111454998B (zh) * 2020-04-21 2023-05-02 黄山科宏生物香料股份有限公司 一种手性羟基酸酯的生物制备方法
CN113430240B (zh) * 2021-06-29 2023-07-04 华东理工大学 一种连续流生物催化合成阿扎那韦中间体氯醇的方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003339387A (ja) 2002-03-19 2003-12-02 Mitsubishi Chemicals Corp 新規カルボニル還元酵素及びこれをコードする遺伝子、ならびにこれらを利用した光学活性アルコールの製造方法
WO2006013801A1 (ja) 2004-08-06 2006-02-09 Kaneka Corporation 新規カルボニル還元酵素、その遺伝子、およびその利用法
WO2011132444A1 (ja) 2010-04-22 2011-10-27 株式会社カネカ 改変型カルボニル還元酵素、その遺伝子、およびそれらを利用した光学活性アルコールの製造方法
WO2015119261A1 (ja) 2014-02-06 2015-08-13 株式会社エーピーアイ コーポレーション ロスバスタチンカルシウム及びその中間体の製造方法
JP2017518744A (ja) 2014-05-09 2017-07-13 凱菜英医藥集團（天津）股▲分▼有限公司Ａｓｙｍｃｈｅｍ Ｌａｂｏｒａｔｏｒｉｅｓ （Ｔｉａｎｊｉｎ） Ｃｏ．， Ｌｔｄ． ジカルボニル還元酵素突然変異体及びその応用

Also Published As

Publication number Publication date
JPWO2021100848A1 (https=) 2021-05-27
CN114729374B (zh) 2024-12-17
CN114729374A (zh) 2022-07-08
WO2021100848A1 (ja) 2021-05-27
EP4063512A4 (en) 2024-01-03
US20230025343A1 (en) 2023-01-26
US12473578B2 (en) 2025-11-18
EP4063512A1 (en) 2022-09-28

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