CN114729374B - 羰基还原酶、编码该酶的核酸、以及利用它们的光学活性化合物的制造方法 - Google Patents

羰基还原酶、编码该酶的核酸、以及利用它们的光学活性化合物的制造方法 Download PDF

Info

Publication number
CN114729374B
CN114729374B CN202080080505.7A CN202080080505A CN114729374B CN 114729374 B CN114729374 B CN 114729374B CN 202080080505 A CN202080080505 A CN 202080080505A CN 114729374 B CN114729374 B CN 114729374B
Authority
CN
China
Prior art keywords
ala
ile
ser
leu
gly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202080080505.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN114729374A (zh
Inventor
井浦崇敦
出来岛康方
阪本刚
原磨理
平冈宏敏
哈拉尔德·格罗热
崔智恩
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
API Corp
Original Assignee
API Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by API Corp filed Critical API Corp
Publication of CN114729374A publication Critical patent/CN114729374A/zh
Application granted granted Critical
Publication of CN114729374B publication Critical patent/CN114729374B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C12P17/167—Heterorings having sulfur atoms as ring heteroatoms, e.g. vitamin B1, thiamine nucleus and open chain analogs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01184—Carbonyl reductase (NADPH) (1.1.1.184)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202080080505.7A 2019-11-22 2020-11-20 羰基还原酶、编码该酶的核酸、以及利用它们的光学活性化合物的制造方法 Active CN114729374B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-211797 2019-11-22
JP2019211797 2019-11-22
PCT/JP2020/043366 WO2021100848A1 (ja) 2019-11-22 2020-11-20 カルボニル還元酵素、これをコードする核酸、及びこれらを利用した光学活性化合物の製造方法

Publications (2)

Publication Number Publication Date
CN114729374A CN114729374A (zh) 2022-07-08
CN114729374B true CN114729374B (zh) 2024-12-17

Family

ID=75981600

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080080505.7A Active CN114729374B (zh) 2019-11-22 2020-11-20 羰基还原酶、编码该酶的核酸、以及利用它们的光学活性化合物的制造方法

Country Status (5)

Country Link
US (1) US12473578B2 (https=)
EP (1) EP4063512A4 (https=)
JP (1) JP7715045B2 (https=)
CN (1) CN114729374B (https=)
WO (1) WO2021100848A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115466755B (zh) * 2022-10-24 2025-07-29 常州佳德医药科技有限公司 一种羰基还原酶不对称还原制备(-)-5-氮杂螺[2,4]庚烷-7-醇的方法
CN115820583B (zh) * 2022-12-29 2026-01-30 金达威生物技术(江苏)有限公司 羰基还原酶突变体及其制备方法、应用和(r)-6-羟基-8-氯辛酸乙酯的制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111454998A (zh) * 2020-04-21 2020-07-28 黄山科宏生物香料股份有限公司 一种手性羟基酸酯的生物制备方法
CN113430240A (zh) * 2021-06-29 2021-09-24 华东理工大学 一种连续流生物催化合成阿扎那韦中间体氯醇的方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57183799A (en) 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid
JPH0279866A (ja) 1988-09-16 1990-03-20 Konica Corp カラー画像形成装置
JP2648897B2 (ja) 1991-07-01 1997-09-03 塩野義製薬株式会社 ピリミジン誘導体
JP3481325B2 (ja) 1994-09-06 2003-12-22 宇部興産株式会社 光学活性な３−オキシ−５−オキソ−６−ヘプテン酸誘導体の製法
DE60323215D1 (de) 2002-03-19 2008-10-09 Mitsubishi Chem Corp Neue carbonylreduktase, diese codierendes gen und verfahren zur produktion optisch-aktiver alkohole damit
JP4270918B2 (ja) * 2002-03-19 2009-06-03 三菱化学株式会社 新規カルボニル還元酵素及びこれをコードする遺伝子、ならびにこれらを利用した光学活性アルコールの製造方法
JP4426437B2 (ja) * 2002-04-30 2010-03-03 株式会社カネカ 新規カルボニル還元酵素、その遺伝子、およびその利用法
JP2005034025A (ja) 2003-07-18 2005-02-10 Mitsubishi Pharma Corp 大腸菌での異種遺伝子発現を促進させるプラスミドベクター、組換え大腸菌、該組換え大腸菌を用いた化学物質製造法及び組換えタンパク質の製造法
JP2007502124A (ja) * 2003-08-11 2007-02-08 コデクシス， インコーポレイテッド 改良ケトレダクターゼポリペプチドおよび関連ポリヌクレオチド
WO2006013801A1 (ja) * 2004-08-06 2006-02-09 Kaneka Corporation 新規カルボニル還元酵素、その遺伝子、およびその利用法
WO2011132444A1 (ja) * 2010-04-22 2011-10-27 株式会社カネカ 改変型カルボニル還元酵素、その遺伝子、およびそれらを利用した光学活性アルコールの製造方法
EP3103878A4 (en) 2014-02-06 2017-08-16 API Corporation Rosuvastatin calcium and process for producing intermediate thereof
CN103966176B (zh) * 2014-05-09 2017-12-22 凯莱英医药集团（天津）股份有限公司 双羰基还原酶突变体及其应用
US10676441B2 (en) 2015-08-05 2020-06-09 Api Corporation Method for producing pitavastatin calcium
CN106243205B (zh) * 2016-08-17 2019-08-02 中国科学院植物研究所 一种降解棒曲霉素的蛋白及其编码基因与应用
EP3652328A1 (en) * 2017-07-14 2020-05-20 c-LEcta GmbH Ketoreductases
JP6828109B2 (ja) 2019-09-10 2021-02-10 キヤノン株式会社 画像形成装置

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111454998A (zh) * 2020-04-21 2020-07-28 黄山科宏生物香料股份有限公司 一种手性羟基酸酯的生物制备方法
CN113430240A (zh) * 2021-06-29 2021-09-24 华东理工大学 一种连续流生物催化合成阿扎那韦中间体氯醇的方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
分子改造羰基还原酶 ＣｐＣＲ 提高其催化合成 ２⁃苯乙醇能力;陈孟军等;食品与发酵工业;第49卷(第19期);30-37 *
定点突变提高羰基还原酶ChKRED03的热稳定性;刘 艳等;应用与环境生物学报;第27卷(第6期);1434-1440 *

Also Published As

Publication number Publication date
JPWO2021100848A1 (https=) 2021-05-27
CN114729374A (zh) 2022-07-08
WO2021100848A1 (ja) 2021-05-27
EP4063512A4 (en) 2024-01-03
US20230025343A1 (en) 2023-01-26
US12473578B2 (en) 2025-11-18
EP4063512A1 (en) 2022-09-28
JP7715045B2 (ja) 2025-07-30

Similar Documents

Publication Publication Date Title
JP2024042079A (ja) 操作されたガラクトースオキシダーゼ改変体酵素
JP7491588B2 (ja) 操作されたパントテン酸キナーゼ改変体酵素
JP7678580B2 (ja) 操作されたスクロースホスホリラーゼバリアント酵素
JP7726528B2 (ja) 操作された酢酸キナーゼバリアント酵素
US7335757B2 (en) Carbonyl reductase, gene encoding the same, and process for producing optically active alcohols using the same
AU2019302422A1 (en) Engineered phosphopentomutase variant enzymes
CN114729374B (zh) 羰基还原酶、编码该酶的核酸、以及利用它们的光学活性化合物的制造方法
JP2023544408A (ja) 操作されたガラクトースオキシダーゼ改変体酵素
JP4205496B2 (ja) 新規カルボニル還元酵素及びこれをコードするｄｎａ、ならびにこれらを利用した光学活性アルコールの製造方法
CN107109446B (zh) 顺式-5-羟基-l-哌可酸的制造方法
JP6844073B1 (ja) （１ｒ，３ｒ）−３−（トリフルオロメチル）シクロヘキサン−１−オール及びその中間体の製造法
JP2024500102A (ja) 操作されたウリジンホスホリラーゼ改変体酵素
JP2025523627A (ja) １，３－ジオール置換インデンの合成のためのケトレダクターゼ酵素
CN116615534A (zh) 工程化泛酸激酶变体酶
JP2023544783A (ja) 操作されたホスホペントムターゼ改変体酵素

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB02 Change of applicant information
CB02 Change of applicant information

Address after: Fukuoka Prefecture

Applicant after: API Corp.

Address before: Tokyo, Japan

Applicant before: API Corp.

GR01 Patent grant
GR01 Patent grant