JP7661486B2 - 細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット - Google Patents

細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット Download PDF

Info

Publication number
JP7661486B2
JP7661486B2 JP2023517468A JP2023517468A JP7661486B2 JP 7661486 B2 JP7661486 B2 JP 7661486B2 JP 2023517468 A JP2023517468 A JP 2023517468A JP 2023517468 A JP2023517468 A JP 2023517468A JP 7661486 B2 JP7661486 B2 JP 7661486B2
Authority
JP
Japan
Prior art keywords
cell culture
region
cell
hydrophilic polymer
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2023517468A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2022230734A5 (https=
JPWO2022230734A1 (https=
Inventor
裕 今泉
純平 門田
豪士 久野
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tosoh Corp
Original Assignee
Tosoh Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tosoh Corp filed Critical Tosoh Corp
Publication of JPWO2022230734A1 publication Critical patent/JPWO2022230734A1/ja
Publication of JPWO2022230734A5 publication Critical patent/JPWO2022230734A5/ja
Priority to JP2025046268A priority Critical patent/JP2025090847A/ja
Application granted granted Critical
Publication of JP7661486B2 publication Critical patent/JP7661486B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C12N2535/10—Patterned coating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2023517468A 2021-04-27 2022-04-20 細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット Active JP7661486B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025046268A JP2025090847A (ja) 2021-04-27 2025-03-21 細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021075266 2021-04-27
JP2021075266 2021-04-27
PCT/JP2022/018285 WO2022230734A1 (ja) 2021-04-27 2022-04-20 細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025046268A Division JP2025090847A (ja) 2021-04-27 2025-03-21 細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット

Publications (3)

Publication Number Publication Date
JPWO2022230734A1 JPWO2022230734A1 (https=) 2022-11-03
JPWO2022230734A5 JPWO2022230734A5 (https=) 2023-11-30
JP7661486B2 true JP7661486B2 (ja) 2025-04-14

Family

ID=83847075

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2023517468A Active JP7661486B2 (ja) 2021-04-27 2022-04-20 細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット
JP2025046268A Pending JP2025090847A (ja) 2021-04-27 2025-03-21 細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025046268A Pending JP2025090847A (ja) 2021-04-27 2025-03-21 細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット

Country Status (6)

Country Link
US (1) US20240218322A1 (https=)
EP (1) EP4317407A4 (https=)
JP (2) JP7661486B2 (https=)
KR (1) KR20230156412A (https=)
CN (1) CN117203320A (https=)
WO (1) WO2022230734A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020062009A (ja) * 2018-10-16 2020-04-23 東ソー株式会社 細胞培養基材、細胞培養基材の製造方法、及びスフェロイドの製造方法
WO2025105395A1 (ja) * 2023-11-15 2025-05-22 東ソー株式会社 細胞培養基材及びその製造方法、並びに細胞培養キット

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009048833A (ja) 2007-08-17 2009-03-05 Sony Corp 燃料電池の製造方法、燃料電池および電子機器
JP2014219261A (ja) 2013-05-08 2014-11-20 コニカミノルタ株式会社 マイクロチャンバーチップの製造方法
WO2019021748A1 (ja) 2017-07-22 2019-01-31 東洋製罐グループホールディングス株式会社 培養容器、培養容器の製造方法、積層構造体、及び積層構造体の製造方法
JP2019110819A (ja) 2017-12-22 2019-07-11 国立大学法人山口大学 細胞接着基材の作製方法
JP2020062009A (ja) 2018-10-16 2020-04-23 東ソー株式会社 細胞培養基材、細胞培養基材の製造方法、及びスフェロイドの製造方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08140673A (ja) 1994-11-17 1996-06-04 W R Grace & Co スフェロイドの作製方法
WO2005118013A1 (ja) * 2004-06-01 2005-12-15 Dai Nippon Printing Co., Ltd. 人工血管およびその製造方法
KR100785049B1 (ko) * 2006-01-11 2007-12-12 한국과학기술연구원 줄기세포로부터 배아체를 형성 및 성장시키는 방법 및 장치
SG11201408620SA (en) * 2012-06-29 2015-01-29 Polymers Crc Ltd Process for modifying a polymeric surface
JP6185816B2 (ja) 2013-10-11 2017-08-23 Ａｇｃテクノグラス株式会社 細胞培養容器
JP2021158960A (ja) * 2020-03-31 2021-10-11 東ソー株式会社 多能性幹細胞から外胚葉系細胞を分化誘導する方法及び製造方法
WO2022039165A1 (ja) * 2020-08-18 2022-02-24 東ソー株式会社 多能性幹細胞の外胚葉、中胚葉及び内胚葉系細胞への分化誘導方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009048833A (ja) 2007-08-17 2009-03-05 Sony Corp 燃料電池の製造方法、燃料電池および電子機器
JP2014219261A (ja) 2013-05-08 2014-11-20 コニカミノルタ株式会社 マイクロチャンバーチップの製造方法
WO2019021748A1 (ja) 2017-07-22 2019-01-31 東洋製罐グループホールディングス株式会社 培養容器、培養容器の製造方法、積層構造体、及び積層構造体の製造方法
JP2019110819A (ja) 2017-12-22 2019-07-11 国立大学法人山口大学 細胞接着基材の作製方法
JP2020062009A (ja) 2018-10-16 2020-04-23 東ソー株式会社 細胞培養基材、細胞培養基材の製造方法、及びスフェロイドの製造方法

Also Published As

Publication number Publication date
JP2025090847A (ja) 2025-06-17
EP4317407A1 (en) 2024-02-07
US20240218322A1 (en) 2024-07-04
EP4317407A4 (en) 2025-07-30
CN117203320A (zh) 2023-12-08
WO2022230734A1 (ja) 2022-11-03
JPWO2022230734A1 (https=) 2022-11-03
KR20230156412A (ko) 2023-11-14

Similar Documents

Publication Publication Date Title
JP2025090847A (ja) 細胞培養基材及びその製造方法、多能性幹細胞の分化誘導方法、並びに細胞培養キット
CN102137924A (zh) 细胞培养制品和筛选
US20210355424A1 (en) Cell culture substrate, method for producing cell culture substrate, and method for producing spheroids
JP2021158960A (ja) 多能性幹細胞から外胚葉系細胞を分化誘導する方法及び製造方法
EP4180515A1 (en) Method for inducing differentiation of pluripotent stem cells into ectodermal, mesodermal, and endodermal cells
WO2019035436A1 (ja) 多能性幹細胞の培養基材及び多能性幹細胞の製造方法
JP2008173018A (ja) 細胞培養方法及び細胞培養用基板
JP7835162B2 (ja) 細胞塊の製造方法
CN116583595A (zh) 培养构件及其用途
Kuno et al. Application of the water-insoluble, temperature-responsive block polymer poly (butyl methacrylate-block-N-isopropylacrylamide) for pluripotent stem cell culture and cell-selective detachment
JP7271870B2 (ja) 多能性幹細胞の培養基材及び多能性幹細胞の製造方法
US20200332252A1 (en) Hollow microcarrier for shear-free culture of adherent cells in bioreactors
JP7807774B2 (ja) 細胞培養基材および細胞培養方法
EP3935149B1 (en) Method for separating cells using a temperature-responsive polymer
JP2020015892A (ja) ブロック共重合体及び培養基材、幹細胞の製造方法
JP2019024349A (ja) 細胞シートの剥離性の調整方法、細胞シートの製造方法、及び細胞培養容器の製造方法
WO2025105395A1 (ja) 細胞培養基材及びその製造方法、並びに細胞培養キット
JP2025158464A (ja) 細胞培養基材、及び細胞培養キット
JP2025158463A (ja) 細胞培養基材の製造方法
JP2022169458A (ja) 細胞培養基材及びその製造方法、並びに細胞培養キット
JP2023178108A (ja) 細胞培養基材
JP7183612B2 (ja) 多能性幹細胞の培養基材及び多能性幹細胞の製造方法
JP2024067168A (ja) 細胞培養基材及びその製造方法
Babaie et al. Imprinted Binary Colloidal Crystals Support Growth and Stemness of Mouse Embryonic Stem Cells
JP2025158462A (ja) 細胞培養基材の製造方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230904

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230904

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240827

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241024

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250204

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20250213

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250402

R150 Certificate of patent or registration of utility model

Ref document number: 7661486

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150