JP7516388B2 - アッセイ測定を実行するための組成物および方法 - Google Patents
アッセイ測定を実行するための組成物および方法 Download PDFInfo
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- JP7516388B2 JP7516388B2 JP2021538813A JP2021538813A JP7516388B2 JP 7516388 B2 JP7516388 B2 JP 7516388B2 JP 2021538813 A JP2021538813 A JP 2021538813A JP 2021538813 A JP2021538813 A JP 2021538813A JP 7516388 B2 JP7516388 B2 JP 7516388B2
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Citations (5)
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|---|---|---|---|---|
| JP2001021560A (ja) | 1999-07-07 | 2001-01-26 | Nitto Denko Corp | 免疫クロマト測定法 |
| JP2009115828A (ja) | 1995-03-10 | 2009-05-28 | Meso Scale Technologies Llc | 多重アレイの多重特異的な電気化学発光試験 |
| JP2014509735A (ja) | 2011-03-16 | 2014-04-21 | ベイジン ユニジャ テクノロジー インコーポレイティド | 電気的化学発光免疫測定法 |
| US20140367278A1 (en) | 2013-06-12 | 2014-12-18 | PharmOptima, LLC | Methods for Detecting Survival Motor Neuron (SMN) Protein in Whole Blood or Cerebral Spinal Fluid |
| CN106526183A (zh) | 2016-09-21 | 2017-03-22 | 宁波大学 | 一种电位分辨双肿瘤标志物的电化学发光免疫传感器的制备方法及其应用 |
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| US5238808A (en) | 1984-10-31 | 1993-08-24 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US5310687A (en) | 1984-10-31 | 1994-05-10 | Igen, Inc. | Luminescent metal chelate labels and means for detection |
| US6316607B1 (en) | 1986-04-30 | 2001-11-13 | Igen International, Inc. | Electrochemiluminescent assays |
| US6165729A (en) | 1986-04-30 | 2000-12-26 | Hyperion Catalysis International, Inc. | Electrochemiluminescent reaction utilizing amine-derived reductant |
| US6451225B1 (en) | 1986-04-30 | 2002-09-17 | Igen International, Inc. | Electrochemiluminescent reaction utilizing amine-derived reductant |
| US5591581A (en) | 1986-04-30 | 1997-01-07 | Igen, Inc. | Electrochemiluminescent rhenium moieties and methods for their use |
| US5147806A (en) | 1988-04-29 | 1992-09-15 | Igen, Inc. | Method and apparatus for conducting electrochemiluminescence measurements |
| US5935779A (en) | 1988-11-03 | 1999-08-10 | Igen International Inc. | Methods for improved particle electrochemiluminescence assay |
| US5308754A (en) | 1988-03-21 | 1994-05-03 | Kankare Jouko J | Electrogenerated luminescence in solution |
| US5093268A (en) | 1988-04-28 | 1992-03-03 | Igen, Inc. | Apparatus for conducting a plurality of simultaneous measurements of electrochemiluminescent phenomena |
| US5705402A (en) | 1988-11-03 | 1998-01-06 | Igen International, Inc. | Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets |
| JPH076913B2 (ja) | 1988-11-03 | 1995-01-30 | イゲン，インコーポレーテッド | アミン誘導還元剤を利用する電気化学発光反応 |
| US5324457A (en) | 1989-10-02 | 1994-06-28 | Board Of Regents, The University Of Tx System | Devices and methods for generating electrogenerated chemiluminescence |
| US5776672A (en) | 1990-09-28 | 1998-07-07 | Kabushiki Kaisha Toshiba | Gene detection method |
| JPH0534345A (ja) | 1991-02-19 | 1993-02-09 | Tdk Corp | 化学発光を利用する抗原・抗体の測定方法 |
| ZA929351B (en) | 1991-12-11 | 1993-06-04 | Igen Inc | Electrochemiluminescent label for DNA assays. |
| US5656504A (en) * | 1992-10-26 | 1997-08-12 | Pharmacia Biosensor Ab | Method of preventing undesired binding in solid phase assays |
| WO1994025853A1 (de) * | 1993-05-03 | 1994-11-10 | Boehringer Mannheim Gmbh | Elektrochemilumineszenzverfahren |
| US5786141A (en) | 1994-08-26 | 1998-07-28 | Bard; Allen J. | Electrogenerated chemiluminescence labels for analysis and/or referencing |
| US5744367A (en) * | 1994-11-10 | 1998-04-28 | Igen International, Inc. | Magnetic particle based electrochemiluminescent detection apparatus and method |
| US5866434A (en) | 1994-12-08 | 1999-02-02 | Meso Scale Technology | Graphitic nanotubes in luminescence assays |
| US5643713A (en) | 1995-06-07 | 1997-07-01 | Liang; Pam | Electrochemiluminescent monitoring of compounds |
| US5641623A (en) | 1995-01-04 | 1997-06-24 | Martin; Mark T. | Electrochemiluminescence assay |
| AU4756296A (en) | 1995-01-06 | 1996-07-24 | Igen, Inc. | Electrogenerated chemiluminescence through enhanced particle luminescence |
| US5993740A (en) | 1995-01-20 | 1999-11-30 | Hitachi, Ltd. | Immunoassay method and analyzer using magnetic particles |
| US6207369B1 (en) | 1995-03-10 | 2001-03-27 | Meso Scale Technologies, Llc | Multi-array, multi-specific electrochemiluminescence testing |
| US6140045A (en) | 1995-03-10 | 2000-10-31 | Meso Scale Technologies | Multi-array, multi-specific electrochemiluminescence testing |
| US5679519A (en) | 1995-05-09 | 1997-10-21 | Oprandy; John J. | Multi-label complex for enhanced sensitivity in electrochemiluminescence assay |
| US6319670B1 (en) | 1995-05-09 | 2001-11-20 | Meso Scale Technology Llp | Methods and apparatus for improved luminescence assays using microparticles |
| US5589136A (en) | 1995-06-20 | 1996-12-31 | Regents Of The University Of California | Silicon-based sleeve devices for chemical reactions |
| GB9606850D0 (en) | 1996-04-01 | 1996-06-05 | Univ Liverpool | An assay system and novel labelled compounds for use therwith |
| FR2764381A1 (fr) | 1997-06-09 | 1998-12-11 | Univ De Neuchatel | Detecteur electrochimioluminescent |
| US6413783B1 (en) | 1997-09-18 | 2002-07-02 | Meso Scale Technologies, Llc | Assay sonication apparatus and methodology |
| US6200531B1 (en) | 1998-05-11 | 2001-03-13 | Igen International, Inc. | Apparatus for carrying out electrochemiluminescence test measurements |
| EP0962773A1 (en) | 1998-06-03 | 1999-12-08 | Mark Howard Jones | Electrochemical based assay processes instrument and labels |
| GB9815042D0 (en) | 1998-07-10 | 1998-09-09 | Imperial College | Detector |
| US6136268A (en) | 1999-08-17 | 2000-10-24 | Orion Diagnostica | Method for luminescence measurements |
| DK1412725T3 (en) | 2001-06-29 | 2019-03-25 | Meso Scale Technologies Llc | Multi-well plates for LUMINESCENSE TEST MEASUREMENTS |
| US6808939B2 (en) | 2001-06-29 | 2004-10-26 | Igen International, Inc. | ECL labels having improved non-specific binding properties, methods of using and kits containing the same |
| AU2008200361B2 (en) | 2001-09-10 | 2010-06-17 | Meso Scale Technologies, Llc. | Assay buffer, compositions containing the same, and methods of using the same |
| WO2003023380A1 (en) | 2001-09-10 | 2003-03-20 | Meso Scale Technologies, Llc | Assay buffer, compositions containing the same, and methods of using the same |
| US20080261243A1 (en) | 2004-10-06 | 2008-10-23 | Wellstat Biologics Corporation | Detection of Elevated Levels of Her-2/Neu Protein on Circulating Cancer Cells and Treatment |
| US20090291920A1 (en) | 2006-04-18 | 2009-11-26 | Wellstat Biologics Corporation | Detection of steroid receptors on circulating carcinoma cells and treatment |
| CN101532961B (zh) | 2009-04-10 | 2011-07-20 | 中国科学院长春应用化学研究所 | 一种电化学发光检测的方法 |
| JP2011047923A (ja) | 2009-07-27 | 2011-03-10 | Panasonic Corp | リポソーム組成物及びその製造方法並びにそれを用いたアナライトの分析方法 |
| US20120190586A1 (en) | 2009-10-15 | 2012-07-26 | Waters Marcey L | Synthetic Receptors for Identification of Protein Posttranslation Modifications |
| EP4060325B1 (en) | 2009-12-07 | 2024-08-21 | Meso Scale Technologies, LLC. | Assay cartridge reader |
| BR112013007614B1 (pt) * | 2010-10-25 | 2020-12-29 | F. Hoffmann-La Roche Ag | método para medir um analito, composição reagente, mistura reagente, uso de uma composição reagente, uso de uma amida de ácido carbônico e kit |
| CA2824404C (en) | 2011-01-06 | 2023-01-03 | Meso Scale Technologies, Llc | Assay cartridges for pcr analysis and methods of use thereof |
| ES2621507T3 (es) | 2012-08-02 | 2017-07-04 | F. Hoffmann-La Roche Ag | Nuevos complejos a base de iridio para EQL |
| JP2015526427A (ja) | 2012-08-02 | 2015-09-10 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Ｅｃｌ用の新規イリジウムベース錯体 |
| CA2904181A1 (en) | 2013-03-13 | 2014-10-09 | Anahit Aghvanyan | Sandwich immunoassay comprising anchoring reagent |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| EP3143401A4 (en) | 2014-05-15 | 2017-10-11 | Meso Scale Technologies, LLC | Improved assay methods |
| WO2017153574A1 (en) | 2016-03-11 | 2017-09-14 | Roche Diagnostics Gmbh | Branched-chain amines in electrochemiluminescence detection |
| CN106124753B (zh) | 2016-07-08 | 2018-03-06 | 广州东林生物科技有限公司 | 电化学发光缓冲液及清洗液 |
| CN109444116A (zh) | 2018-10-17 | 2019-03-08 | 江西中洪博元生物技术有限公司 | 一种增强型ecl化学发光试剂 |
| EP4176263A1 (en) | 2020-07-01 | 2023-05-10 | Meso Scale Technologies, LLC | Compositions and methods for assay measurements |
-
2019
- 2019-12-23 AU AU2019418498A patent/AU2019418498A1/en active Pending
- 2019-12-23 SG SG11202106924VA patent/SG11202106924VA/en unknown
- 2019-12-23 WO PCT/US2019/068293 patent/WO2020142313A1/en not_active Ceased
- 2019-12-23 EP EP19842531.6A patent/EP3906412A1/en active Pending
- 2019-12-23 US US17/264,489 patent/US12203937B2/en active Active
- 2019-12-23 KR KR1020217024461A patent/KR102897648B1/ko active Active
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- 2021-06-25 ZA ZA2021/04404A patent/ZA202104404B/en unknown
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-
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-
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- 2024-04-25 JP JP2024071628A patent/JP7805391B2/ja active Active
- 2024-12-06 US US18/971,325 patent/US20250102511A1/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009115828A (ja) | 1995-03-10 | 2009-05-28 | Meso Scale Technologies Llc | 多重アレイの多重特異的な電気化学発光試験 |
| JP2001021560A (ja) | 1999-07-07 | 2001-01-26 | Nitto Denko Corp | 免疫クロマト測定法 |
| JP2014509735A (ja) | 2011-03-16 | 2014-04-21 | ベイジン ユニジャ テクノロジー インコーポレイティド | 電気的化学発光免疫測定法 |
| US20140367278A1 (en) | 2013-06-12 | 2014-12-18 | PharmOptima, LLC | Methods for Detecting Survival Motor Neuron (SMN) Protein in Whole Blood or Cerebral Spinal Fluid |
| CN106526183A (zh) | 2016-09-21 | 2017-03-22 | 宁波大学 | 一种电位分辨双肿瘤标志物的电化学发光免疫传感器的制备方法及其应用 |
Non-Patent Citations (5)
| Title |
|---|
| Kirschbaum-Harriman, Stefanie，Improving ruthenium-based ECL through nonionic surfactants and tertiary amines，Analyst，2017年05月25日，Vol.142，pp.2648-2653，https://doi.org/10.1039/C7AN00197E |
| Kirschbaum-Harriman, Stefanie，Signal enhancement and low oxidation potentials for miniaturized ECL biosensors via N-butyldiethanolamine，Analyst，2017年05月15日，142.13，pp.2469-2474，https://doi.org/10.1039/C7AN00261K |
| Kitte, S.A.，Electrogenerated chemiluminescence of tris(2,2'-bipyridine)ruthenium(II) using N-(3-aminopropyl)diethanolamine as coreactant.，Anal Bioanal Chem，2016年03月04日，408，pp.7059-7065，https://doi.org/10.1007/s00216-016-9409-z |
| Shuang Han，Effect of hydroxyl and amino groups on electrochemiluminescence activity of tertiary amines at low tris(2,2′-bipyridyl)ruthenium(II) concentrations,，Talanta,，2010年04月15日，Vol.81, 1-2，pp.44-47，https://doi.org/10.1016/j.talanta.2009.11.037. |
| Yu Chen，A simple and versatile paper-based electrochemiluminescence biosensing platform for hepatitis B virus surface antigen detection，Biochemical Engineering Journal，2018年01月15日，Vol.129，pp.1-6，https://doi.org/10.1016/j.bej.2017.10.012. |
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| TW202438888A (zh) | 2024-10-01 |
| JP7805391B2 (ja) | 2026-01-23 |
| US20220018846A1 (en) | 2022-01-20 |
| US20220373554A1 (en) | 2022-11-24 |
| US11927592B2 (en) | 2024-03-12 |
| TW202043771A (zh) | 2020-12-01 |
| TWI871299B (zh) | 2025-02-01 |
| AU2019418498A1 (en) | 2021-07-15 |
| SG11202106924VA (en) | 2021-07-29 |
| KR20210146884A (ko) | 2021-12-06 |
| KR20250037585A (ko) | 2025-03-17 |
| US12203937B2 (en) | 2025-01-21 |
| CA3125146A1 (en) | 2020-07-09 |
| ZA202104404B (en) | 2025-09-25 |
| JP2022515917A (ja) | 2022-02-22 |
| CN113366313A (zh) | 2021-09-07 |
| KR102897648B1 (ko) | 2025-12-08 |
| WO2020142313A1 (en) | 2020-07-09 |
| IL284558A (en) | 2021-08-31 |
| US20250102511A1 (en) | 2025-03-27 |
| JP2024105355A (ja) | 2024-08-06 |
| EP3906412A1 (en) | 2021-11-10 |
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