JP7509745B2 - ポリヌクレオチド合成方法、キット、およびシステム - Google Patents

ポリヌクレオチド合成方法、キット、およびシステム Download PDF

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JP7509745B2
JP7509745B2 JP2021500840A JP2021500840A JP7509745B2 JP 7509745 B2 JP7509745 B2 JP 7509745B2 JP 2021500840 A JP2021500840 A JP 2021500840A JP 2021500840 A JP2021500840 A JP 2021500840A JP 7509745 B2 JP7509745 B2 JP 7509745B2
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nucleotide
strand
polynucleotide
cycle
supporting
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ミルトン,ジョン
ネイヤール,ソビア
リードル,ヤン
亮祐 大柿
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オックスフォード ナノポール テクノロジーズ ピーエルシー
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2021500840A 2018-07-19 2019-07-19 ポリヌクレオチド合成方法、キット、およびシステム Active JP7509745B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1811811.7A GB201811811D0 (en) 2018-07-19 2018-07-19 Method
GB1811811.7 2018-07-19
PCT/GB2019/052036 WO2020016605A1 (en) 2018-07-19 2019-07-19 Polynucleotide synthesis method, kit and system

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JP2021529548A JP2021529548A (ja) 2021-11-04
JP2021529548A5 JP2021529548A5 (https=) 2022-06-30
JP7509745B2 true JP7509745B2 (ja) 2024-07-02

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US (2) US12037636B2 (https=)
EP (1) EP3823755A1 (https=)
JP (1) JP7509745B2 (https=)
CN (1) CN112423885B (https=)
AU (1) AU2019307836B2 (https=)
CA (1) CA3104698A1 (https=)
GB (1) GB201811811D0 (https=)
WO (1) WO2020016605A1 (https=)

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GB201811810D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811813D0 (en) * 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
US20230117083A1 (en) * 2020-01-22 2023-04-20 Nuclera Nucleics Ltd. Methods of nucleic acid synthesis
GB202013102D0 (en) * 2020-08-21 2020-10-07 Nuclera Nucleics Ltd Methods of nucleic acid synthesis
GB202309649D0 (en) * 2023-06-27 2023-08-09 Oxford Nanopore Tech Plc Method

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JP2021529549A (ja) 2018-07-19 2021-11-04 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチドの合成法、キット、およびシステム
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WO2001088173A2 (en) 2000-05-16 2001-11-22 Hercules Incorporated Methods for the enzymatic assembly of polynucleotides and identification of polynucleotides having desired characteristics
JP2007532120A (ja) 2004-04-14 2007-11-15 オリソン、ヘノミクス、ソシエダッド、アノニマ 核酸分子のサブセットを選択的に検出するための方法
JP2014504153A (ja) 2010-11-12 2014-02-20 ジェン9・インコーポレイテッド 核酸合成のための方法およびデバイス
US20130085073A1 (en) 2011-09-29 2013-04-04 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nuclleic acids synthesis and sequencing
JP2016515391A (ja) 2013-10-15 2016-05-30 モレキュラー アセンブリー, エルエルシー 核酸を合成するための方法および装置
WO2017223517A1 (en) 2016-06-24 2017-12-28 The Regents Of The University Of California Nucleic acid synthesis and sequencing using tethered nucleoside triphosphates
JP2020506687A (ja) 2017-01-19 2020-03-05 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチド分子を合成するための方法および試薬
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JP2021529549A (ja) 2018-07-19 2021-11-04 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチドの合成法、キット、およびシステム
JP2021529551A (ja) 2018-07-19 2021-11-04 オックスフォード ナノポール テクノロジーズ リミテッド ポリヌクレオチドの合成法、キット、およびシステム

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US20250084468A1 (en) 2025-03-13
WO2020016605A1 (en) 2020-01-23
GB201811811D0 (en) 2018-09-05
US12037636B2 (en) 2024-07-16
EP3823755A1 (en) 2021-05-26
CA3104698A1 (en) 2020-01-23
CN112423885A (zh) 2021-02-26
AU2019307836A1 (en) 2021-01-07
US20220213537A1 (en) 2022-07-07
CN112423885B (zh) 2023-07-04
AU2019307836B2 (en) 2024-04-04
JP2021529548A (ja) 2021-11-04

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